Download or read book Molecular Biology Laboratory Protocols Plant Genotyping written by Ghislain, M. and published by International Potato Center. This book was released on with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Genotyping written by Yuri Shavrukov and published by Humana. This book was released on 2024-02-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy and many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genotyping: Methods and Protocols offers researchers the opportunity to update their knowledge and choose the most suitable method of plant genotyping for their chosen application.
Download or read book Genotyping written by Stefan J. White and published by Humana. This book was released on 2018-06-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.
Download or read book Plant Genomics written by Wolfgang Busch and published by Humana Press. This book was released on 2017-04-25 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genomics: Methods and Protocols aims to serve as an inspiration for further studies in plant genomics.
Download or read book High Throughput Plant Phenotyping written by Argelia Lorence and published by Springer Nature. This book was released on 2022-07-27 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume looks at a collection of the latest techniques used to quantify the genome-by-environment-by-management (GxExM) interactions in a variety of model and plant crops. The chapters in this book are organized into five parts. Part One discusses high-throughput plant phenotyping (HTPP) protocols for plants growing under controlled conditions. Part Two present novel algorithms for extracting data from seed images, color analysis from fruits, and other digital readouts from 2D objects. Part Three covers molecular imaging protocols using PET and X-ray approaches, and Part Four presents a collection of HTPP techniques for crops growing under field conditions. The last part focuses on molecular analysis, metabolomics, network analysis, and statistical methods for the quantitative genetic analysis of HTP data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, High-Throughput Plant Phenotyping: Review and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this important field. Chapter 21 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Plant Cytogenetics and Cytogenomics written by Tony Heitkam and published by Springer Nature. This book was released on 2023-07-21 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome fixation, preparation, and manipulation, banding and staining techniques, in situ hybridization, as well as numerous methods that integrate cytogenetics with bioinformatics and computational genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal resource for plant scientists interested in molecular and evolutionary biology, breeding, systematics, and plant -omics in general.
Download or read book Plant Functional Genomics written by Jose M. Alonso and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.
Download or read book Protocols used in Molecular Biology written by Sandeep Singh and published by Bentham Science Publishers. This book was released on 2020-01-23 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
Download or read book Biolistic DNA Delivery in Plants written by Sachin Rustgi and published by Humana. This book was released on 2020-04-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.
Download or read book Plant Gene Regulatory Networks written by Kerstin Kaufmann and published by . This book was released on 2017 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Cytogenetics written by Shahryar F. Kianian and published by Humana. This book was released on 2018-06-09 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics.
Download or read book Plant Genome Engineering written by Bing Yang and published by Springer Nature. This book was released on 2023-03-30 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Genome Engineering: Methods and Protocols is a valuable tool for researchers interested in learning more about this developing and important field.
Download or read book Plant Functional Genomics written by Fatemeh Maghuly and published by Springer Nature. This book was released on with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Crop Breeding written by Delphine Fleury and published by . This book was released on 2014 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Chemical Genomics written by Glenn R. Hicks and published by . This book was released on 2021 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes. Chapters in this book cover topics such as screening plants for novel bioactive chemicals including lipid signaling and photo receptors; small molecule screens to include peptide ligands to generate new variations; using new chemicals to affect and dissect hormone signaling; and the application of easier microscale methods to simplify target identification and validation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Chemical Genomics: Methods and Protocols, Second Edition is a valuable resource that provides a foundation of techniques for novice and expert researchers in the plant chemical biology community.
Download or read book Low Cost Methods for Molecular Characterization of Mutant Plants written by Bradley J. Till and published by Springer. This book was released on 2015-05-08 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Download or read book Plant Reverse Genetics written by Andy Pereira and published by Humana Press. This book was released on 2010-10-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.
