Download or read book Modes of Action of Non pathogenic Fusarium Oxysporum Endophytes for Bio enhancement of Banana Toward Radopholus Similis written by Thi-Thanh-Tam Vu and published by Cuvillier Verlag. This book was released on 2005 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Defence Responses Against Radopolus Similis in East African Highland Bananas EAHB AAA Inoculated with Endophytic Non pathogenic Fusarium Oxysporum written by Pamela Paparu and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In the interactions between fungal endophytes and their hosts, the host may benefit through protection against pathogens and pests, growth promotion and tolerance to abiotic stresses. Non-pathogenic Fusarium oxysporum endophytes of banana have been shown to reduce the damage caused by the Cosmopolitus sordidus and the burrowing nematode Radopholus similis. The mode of protection against the burrowing nematode involves induced resistance, but the molecular basis of this resistance yet to be demonstrated. It has further been reported that protection of the host by multiple endophytes can lead to better control of target pests, probably because of the multiple modes of action involved. This phenomenon, however, has not been fully demonstrated for F. oxysporum endophytes of banana. This study aimed to investigate the molecular and biochemical basis of endophyte protection of East African Highland bananas (EAHB) against C. sordidus and R. similis. Expression of banana defence-related genes following endophyte inoculation and R. similis challenge varied greatly between the nematode-susceptible cv Nabusa and the nematode-tolerant cv Kayinja. In cv Nabusa, only the peroxidase (POX) and lectin genes were responsive to endophyte colonization of roots, or R. similis challenge. POX and lectin activities were significantly down-regulated 2 and 33 days after endophyte inoculation (dai), respectively. In cv Kayinja, endophyte colonization resulted in transient up-regulation of POX and a down-regulation of endochitinase (PR-3), lectin, pectin acetylesterase (PAE), phenylalanine ammonia-lyase (PAL) and PIR7A (peroxidase). Similar to systemic acquired resistance, PR-1 and catalase activities were up-regulated in the cv Kayinja 33 dai. Genes involved in signal transduction, cell wall strengthening, jasmonic acid pathway and defence molecule transport were differentially expressed in endophyte-inoculated plants. The expression profiles of four defence-related genes following endophyte inoculation and R. similis challenge were studied using quantitative real-time PCR. ABC transporter, ; -1,3-glucan synthase, coronatine insensitive 1 (COI1) and lipoxygenase (LOX) were up-regulated following endophyte inoculation. ; -1,3-glucan synthase and COI1 were highly up-regulated following R. similis challenge of endophyte-inoculated plants of the susceptible cv Nabusa, while COI1 and LOX were highly up-regulated following nematode challenge of endophyte-inoculated plants of the tolerant cv Kayinja. However ABC transporter gene activity was not up-regulated following nematode challenge of plants of both cultivars. UP-regulation of phenylpropanoid pathway enzymes PAL, POX and PPO has been observed in roots following colonization by both pathogenic and non-pathogenic fungi. In the current study, endophyte inoculation resulted in down-regulation of PAL activity in both a susceptible (cv Nabusa) and tolerant (cv Yangambi) banana. In cv Nabusa, endophyte inoculation primed PAL activity for up-regulation 30 days post nematode challenge (dpnc). However, in cv Yangambi PAL activity was up-regulated 7 dpnc irrespective of endophyte inoculation. Endophyte inoculation transiently up-regulated POX in cv Nabusa, but activity reduced to the levels in the controls 30 dai. Similar to PAL, R. similis challenge of endophyte-inoculated plants of Nabusa caused significant up-regulation of POX 7 dpnc. Nematode challenge of control plants of cv Yangambi resulted in a non-significant up-regulation of POX compared with non-challenged controls, but a significant up-regulation compared to all endophyte-inoculated plants. PPO activity was transiently up-regulated in cv Nabusa and down-regulated in cv Yangambi 7 dai. For all treatments, PPO activity was significantly reduced between 7 dai and 120 dai (60 dpnc). Fusarium oxysporum endophyte isolates Emb2.4o and V5w2 were successfully marked with benomyl- and chlorate resistance and transformed with fluorescent protein genes, while Eny1.31i, Eny7.11o and V4w5 were marked with benomyl resistance only. Most mutants and fluorescent protein transformants maintained resistance to the selective chemical on PDA and after plant colonization. Benomyl- and chlorate-resistant mutants were successfully used to determine actual plant colonization percentages by inoculated endophytes. Similarly, GFP transformants were successfully used to ascertain the pattern of endophytic root colonization in vivo. In plants dually inoculated with isolates Emb2.4o BR 8 and V5w2 CHR 9, both isolates were recovered from roots and rhizomes 4 weeks after inoculation, but isolate V5w2 CHR 9 proved a better colonizer of the two tissue types. Root colonization by isolate V5w2 CHR 9 was boosted when inoculated dually with Emb2.4o BR 8, while that by Emb2.4o BR 8 was reduced in the presence of V5w2 CHR 9. Where growth advantages were observed for dually inoculated plants, it occurred where plants were challenged with R. similis. In the absence of pests, control plants showed better growth than endophyte-inoculated plants. On the other hand, weevil challenge of control plants resulted in significant reductions in plant height, number of live roots and root fresh weight. Dual endophyte inoculation resulted in a significant reduction in R. similis populations in nematode only challenged plants, compared with plants inoculated with Emb2.4o BR 8 singly and control plants challenged with the nematode. In one replicate banana weevil damage to the outer and inner pseudostem base, and the inner rhizome were significantly reduced for dually-inoculated plants. Copyright.
Download or read book Importance of the Mutualistic Endophyte Fusarium Oxysporum 162 for Enhancement of Tomato Transplants and the Biological Control of the Root knot Nematode Meloidogyne Incognita with Particular Reference to Mode of action written by Abd El-Fattah Adnan Saʼd Dababat and published by Cuvillier Verlag. This book was released on 2007 with total page 119 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Novel Biological and Biotechnological Applications in Plant Nematode Management written by Mujeebur Rahman Khan and published by Springer Nature. This book was released on 2023-12-10 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volume focuses on novel and effective biological and biotechnological methods for managing major plant parasitic nematodes in economically important agricultural crops. Nematodes develop a wide variety of interactions with soil microbes and host plants, and cause enormous losses in crop yields equivalent to around USD 173 billion annually. In view of growing concern for pesticide contamination in crops, coupled with ban on several efficacious pesticides, and increasing demand for organic foods, the biological and biotechnological approaches offer a good alternative to chemicals for managing nematode infestations in agricultural crops. The book embodies twenty-two chapters which are arranged in two groups. The first group covers different novel methods of nematode management such as biotechnological, biopesticides, microbial consortia, host resistance, omics technology, transgenics, nano-nematicides, nano-diagnosis, etc. The second groups of chapters describe important nematode problems in major crops such as cereals, vegetables, pulses, spices, wood yielding conifers, etc. Overall, the book collates the latest information on above topics and offers practical solutions to the limitations and challenges in the existing management technologies. This book is of interest and serves up-to-date and elaborated information to agriculture researchers, teachers, scientists, under-graduates, post-graduates, plant nematologists, plant pathologists, plant protectionists, agronomists, horticulturalists, helminthologists, extension workers, and NGOs.
Download or read book Interrelationships Between Microbial Antagonists Having Divergent Modes of action and Their Influence on Biological Control of Plant parasitic Nematodes written by Alexander Rafael Mendoza Luna and published by Cuvillier Verlag. This book was released on 2008 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biology of Microfungi written by De-Wei Li and published by Springer. This book was released on 2016-03-18 with total page 651 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book includes 24 chapters written by a group of experts in the different fields of microfungi and cover a broad range of topics on microfungi. It provides the most updated information on the latest development in systematics and taxonomy of microfungi, new techniques which were developed in the last ten years and their application in microfungal research. After the International Code of Nomenclature for algae, fungi, and plants (Melbourne Code) was adopted by the Eighteenth International Botanical Congress Melbourne, Australia, July 2011, it has had a profound impact on mycology and its research. Fungal nomenclature changes and its significance to fungal taxonomy and naming of microfungi in the future is discussed in detail. Since dual names system for fungi developing both sexual and asexual states, and fungi developing only asexual state is no longer available, the first five chapters will clarify some confusion and provides perspective views on the direction for future research. The next nine chapters cover microfungi and their ecological roles or functions in the different habitats (air, indoor, aquatic, marine, plants, soils, etc). The remaining 13 chapters cover the relationship of microfungi and humans (good and bad) and usage or application microfungi in different industries, such as food, agriculture, forestry, green technology, pharmaceutics, and medicine, as well as in our daily life. The book bridges the gap between basic mycological research and applied mycology and provide readers a unique set of information and knowledge of microfungi generated from multiple angles in different fields of mycology.
Download or read book Presence and Frequency of Occurrence of Plant Parasitic Nematodes on Coffee Coffea Arabica L Rubiaceae in Ethiopia and the Importance of Endophytic Microrganisms microorganisms for Biocontrol written by Tesfamariam Mekete Mengistu and published by Cuvillier Verlag. This book was released on 2008 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mycorrhiza Eco Physiology Secondary Metabolites Nanomaterials written by Ajit Varma and published by Springer. This book was released on 2017-08-02 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the fourth updated and revised edition of a well-received book that emphasises on fungal diversity, plant productivity and sustainability. It contains new chapters written by leading experts in the field. This book is an up-to-date overview of current progress in mycorrhiza and association with plant productivity and environmental sustainability. The result is a must hands-on guide, ideally suited for agri-biotechnology, soil biology, fungal biology including mycorrhiza and stress management, academia and researchers. The topic of this book is particularly relevant to researchers involved in mycorrhiza, especially to food security and environmental protection. Mycorrhizas are symbioses between fungi and the roots of higher plants. As more than 90% of all known species of plants have the potential to form mycorrhizal associations, the productivity and species composition and the diversity of natural ecosystems are frequently dependent upon the pre sence and activity of mycorrhizas. The biotechnological application of mycorrhizas is expected to promote the production of food while maintaining ecologically and economically sustainable production systems.
Download or read book Ecological Studies on Paecilomyces Lilacinus Strain 251 and Their Importance for Biocontrol of Plant parasitic Nematodes and Environmental Risk Assessment written by Christos Roumpos and published by Cuvillier Verlag. This book was released on 2005 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biological Enhancement of Banana Tissue Culture Plantlets with Endophytic Fungi for the Control of the Burrowing Nematode Radopholus Similis and the Panama Disease Fusarium Oxysporum F sp Cubense written by Luis Ernesto Pocasangre Enamorado and published by . This book was released on 2000 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Significance of non pathogenic isolates of Fusarium oxysporum Schlecht Fries for the biological control of the burrowing nematode Radopholus similis Cobb Thorne on tissue cultured banana written by Björn Ilja Niere and published by . This book was released on 2001 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Host endophyte pest Interactions of Endophytic Fusarium Oxysporum Antagonistic to Radopholus Similis i in Banana Musa Spp written by Shahasi Yusuf Athman and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Radopholus similis is one of the key pests of banana in the East African Highlands and in the world. Although control of this pest has mainly relied on the use of clean planting material, re-infestation of plants in the field remains a critical concern. Alternative management options such as the use of fungal endophytes may be used to provide protection and extend plant life. In this study, a total of 35 endophytic Fusarium isolates were screened for the production of secondary metabolites antagonistic to R. similis in culture. Undiluted and diluted culture filtrates were tested against motile stages of R. similis and eggs. Tests were conducted using culture filtrates of the fungal isolates grown in banana corm broth. All isolates tested demonstrated some level of in vitro antagonistic activity on the mobility of R. similis mixed stages (males, females and juveniles). The percentage of immobilized nematodes increased with increase in the length of exposure time to culture filtrates. After 24 hrs exposure, up to 100% of nematodes were immobilized compared to 26.5% in control treatments. Mortality of mixed stages of R. similis exposed to culture filtrates for 24 hrs, followed by rinsing with sterile distilled water, demonstrated that the effects of culture filtrate treatment were irreversible, as nematodes did not recover and were considered dead. Nematode mortality rates after 24-hr exposure periods ranged from 76.4% to 100.0%. The effects of culture filtrates on R. similis motile stages increased with increasing culture filtrate concentration. Culture filtrates also demonstrated inhibitory effects on hatching of R. similis eggs. Radopholus similis males were more sensitive to culture filtrate treatment than females. The results obtained demonstrate the potential for using endophytic Fusarium isolates as biological control agents against R. similis and/or toxic derivatives as potential nematicides against the nematode.
Download or read book Biological Control of Plant Parasitic Nematodes written by Keith Davies and published by Springer Science & Business Media. This book was released on 2011-06-27 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The offered volume intends to review the biological control theme of phytonematodes from several prospects: ecological; applicative as well as commercial state of the art; understanding the mode-of-action of various biocontrol systems; interaction between the plant host, nematodes’ surface and microorganism’s; candidates for biocontrol; extrapolation of the wide knowledge existed in another systems for understanding biocontrol processes: C. elegans as a model and lessons from other natural systems; and exploiting advanced genomic tools to promote understanding biocontrol processes and thereafter improve specific biological control agents.
Download or read book Fusarium Wilt of Banana written by Randy C. Ploetz and published by American Phytopathological Society. This book was released on 1990 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fusarium wilt of banana: some history and current status of the disease; Importante of fusarium wilt in different banana-growing regions; Taxonomy of fungi in the genus fusarium with emphasis on fusarium oxysporum; Genetic exchange within sexual and asexual populations of the genus fusarium; Molecular genetics of plant pathogenic fusarium oxysporum; Using karyotype variability to investigate the origins and relatednes of isolates of fusarium oxysporum f. sp. cubense; Population biology of fusarium oxysporum f. sp. cubense; Biological control of diseases caused by fusarium oxysporum; Influence of mineral nutrition on fusarium wilt: a proposed mechanism involving cell water relations; Host responses to the pathogen; Banana breeding and fusarium wilt; Breeding bananas and plantains for resistance to fusariu m wilt: the track record; Somaclonal resistance in cavendish banana to fusarium wilt; Baseline tissue and cell culture studies for use in banana improvement schemes.
Download or read book Proceedings of the First International Conference on Banana and Plantain for Africa written by Eldad B. Karamura and published by . This book was released on 2000 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biotechnology and Biology of Trichoderma written by Vijai G. Gupta and published by Newnes. This book was released on 2014-02-17 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology and Biology of Trichoderma serves as a comprehensive reference on the chemistry and biochemistry of one of the most important microbial agents, Trichoderma, and its use in an increased number of industrial bioprocesses for the synthesis of many biochemicals such as pharmaceuticals and biofuels. This book provides individuals working in the field of Trichoderma, especially biochemical engineers, biochemists and biotechnologists, important information on how these valuable fungi can contribute to the production of a wide range of products of commercial and ecological interest. Provides a detailed and comprehensive coverage of the chemistry, biochemistry and biotechnology of Trichoderma, fungi present in soil and plants Includes most important current and potential applications of Trichoderma in bioengineering, bioprocess technology including bioenergy & biofuels, biopharmaceuticals, secondary metabolites and protein engineering Includes the most recent research advancements made on Trichoderma applications in plant biotechnology and ecology and environment
