Download or read book Modern Methods of Steroid Analysis written by Erich Heftmann and published by Elsevier. This book was released on 2012-12-02 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Methods of Steroid Analysis reviews modern methods of steroid analysis such as liquid column chromatography, mass spectrometry, and gas chromatography. Topics covered include qualitative and quantitative analysis of plant sterols by gas-liquid chromatography; Raman spectroscopy of steroids; nuclear magnetic resonance; and applications of lanthanide shift reagents. A computerized method for rapid comparison and retrieval of infrared spectral data is also described. This volume is comprised of 22 chapters and begins with an analysis of hormonal steroids using liquid column chromatography, followed by a discussion on the separation of insect molting hormones using high-pressure liquid chromatography. The reader is methodically introduced to the application of gradient elution and thin-layer chromatography to the analysis of corticosteroids and 17-ketosteroids, as well as some aspects of mass spectrometry in steroid analysis. Subsequent chapters explore other methods such as optical rotatory dispersion and circular dichroism, along with radioisotope techniques. In particular, the radioimmunoassay of plasma steroid hormones and plasma aldosterone is considered. This book should be of interest to biochemists.
Download or read book The Biology of Cholesterol and Related Steroids written by N. B. Myant and published by Butterworth-Heinemann. This book was released on 2014-04-24 with total page 925 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Biology of Cholesterol and Related Steroids focuses on the study of sterols in relation to living organisms. The publication first takes a look at the analysis of sterols and related steroids and the distribution of sterols and related steroids in nature, as well as the processes of extraction and separation and presence of sterols in plants, fungi, vertebrates, and invertebrates. The text then ponders on biosynthesis of sterols and metabolism of cholesterol. Topics include formation of fatty acid esters of cholesterol, steroid hormones, biosynthetic pathway to sterols, reaction mechanisms, and comparative aspects of sterol synthesis. The manuscript examines the developmental aspects of cholesterol metabolism and sterols in biological membranes. The book also reviews cholesterol synthesis in animal tissues, sterol metabolism in isolated cells, and epidemiology of the plasma cholesterol. Discussions focus on selection of statistical populations, genetic influences, regulation of sterol synthesis, general aspects of sterol metabolism, and removal of cell cholesterol in vivo. The publication is a dependable source of data for biochemists and readers interested in the biology of cholesterol and steroids.
Download or read book LC MS in Drug Bioanalysis written by Q. Alan Xu and published by Springer Science & Business Media. This book was released on 2012-07-15 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical pharmacology plays an important role in today’s medicine. Due to the high sensitivity, selectivity, and affordability of a mass spectrometer (MS), the high performance liquid chromatography – mass spectrometry (LC-MS) analytical technique is widely used in the determination of drugs in human biological matrixes for clinical pharmacology. Specifically, LC-MS is used to analyze: anticancer drugs antidementia drugs antidepressant drugs antiepileptic drugs antifundal drug antimicrobial drugs antipsychotic drugs antiretroviral drugs anxiolytic/hypnotic drugs cardiac drugs drugs for addiction immunosuppressant drugs mood stabilizer drugs This book will primarily cover the various methods of validation for LC-MS techniques and applications used in modern clinical pharmacology.
Download or read book Steroid Analysis written by Hugh L. J. Makin and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 737 pages. Available in PDF, EPUB and Kindle. Book excerpt: reviews of the first edition -- `...this is an excellent, comprehensive book and can be highly recommended to those who want an up-to-date reference on steroid analysis.' Analyst
Download or read book Quantitative Analysis of Steroids written by S. Görög and published by Elsevier. This book was released on 2012-12-02 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies in Analytical Chemistry, Volume 5: Quantitative Analysis of Steroids covers the pharmaceutical aspects of the analysis of steroid hormones. This book is divided into nine chapters that examine the biological-clinical analysis of other important groups of steroids, including sterols, vitamin D, bile acids, cardiac glycosides, and sapogenins. The material in the book is classified according to the main groups of steroids, individual chapters being devoted to sex hormones, corticosteroids, sterols, vitamins D, bile acids, cardiac glycosides, sapogenins, and miscellaneous steroids. The structure of each chapter is the same. In section 1 the fundamentals of the chemistry of the group of steroids in question are outlined. Section 2 provides a detailed description of the use of various spectroscopic, chromatographic, protein-binding and other methods, while Section 3 deals with the main problems encountered in the analysis of the group of steroids in question and with their solution using the methods described in Section 2. This book will prove useful to steroid and analytical chemists.
Download or read book Targeted Biomarker Quantitation by LC MS written by Naidong Weng and published by John Wiley & Sons. This book was released on 2017-07-31 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book to offer a blueprint for overcoming the challenges to successfully quantifying biomarkers in living organisms The demand among scientists and clinicians for targeted quantitation experiments has experienced explosive growth in recent years. While there are a few books dedicated to bioanalysis and biomarkers in general, until now there were none devoted exclusively to addressing critical issues surrounding this area of intense research. Target Biomarker Quantitation by LC-MS provides a detailed blueprint for quantifying biomarkers in biological systems. It uses numerous real-world cases to exemplify key concepts, all of which were carefully selected and presented so as to allow the concepts they embody to be easily expanded to future applications, including new biomarker development. Target Biomarker Quantitation by LC-MS primarily focuses on the assay establishment for biomarker quantitation—a critical issue rarely treated in depth. It offers comprehensive coverage of three core areas of biomarker assay establishment: the relationship between the measured biomarkers and their intended usage; contemporary regulatory requirements for biomarker assays (a thorough understanding of which is essential to producing a successful and defendable submission); and the technical challenges of analyzing biomarkers produced inside a living organism or cell. Covers the theory of and applications for state-of-the-art mass spectrometry and chromatography and their applications in biomarker analysis Features real-life examples illustrating the challenges involved in target biomarker quantitation and the innovative approaches which have been used to overcome those challenges Addresses potential obstacles to obtain effective biomarker level and data interpretation, such as specificity establishment and sample collection Outlines a tiered approach and fit-for-purpose assay protocol for target biomarker quantitation Highlights the current state of the biomarker regulatory environment and protocol standards Target Biomarker Quantitation by LC-MS is a valuable resource for bioanalytical scientists, drug metabolism and pharmacokinetics scientists, clinical scientists, analytical chemists, and others for whom biomarker quantitation is an important tool of the trade. It also functions as an excellent text for graduate courses in pharmaceutical, biochemistry and chemistry.
Download or read book Phytochemical Methods A Guide to Modern Techniques of Plant Analysis written by A.J. Harborne and published by Springer Science & Business Media. This book was released on 1998-04-30 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: This long awaited third edition of Phytochemical Methods is, as its predecessors, a key tool for undergraduates, research workers in plant biochemistry, plant taxonomists and any researchers in related areas where the analysis of organic plant components is key to their investigations. Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This latest edition includes descriptions of the most up-to-date methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils. After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes. It also includes an important bibliographic guide to specialized texts. This comprehensive book constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of experimental techniques so that students and researchers can become familiar with these invaluable methods.
Download or read book Applications of Infrared Raman and Resonance Raman Spectroscopy in Biochemistry written by Frank S. Parker and published by Springer Science & Business Media. This book was released on 1983-10-01 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chromatography of Steroids written by and published by Elsevier. This book was released on 2011-09-22 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography of Steroids
Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although not the only volume in this series in which lipids are discussed, the present volume is devoted entirely to methods for the study of membrane lipids. Even now, when membrane proteins are properly receiving so much attention, this emphasis on membrane lipids is appropriate. Essentially all of the phospholipids and sterols of cells are in membranes. Moreover, although membrane proteins are certainly of utmost importance, the more we learn about the functional properties of membrane proteins, the more we appreciate the unique features of phospholipids, without which biological membranes would be impossible. The hydrophobic-hydrophilic duality of phospholipids allows, indeed requires, their association, in an aqueous environment, into an essentially two-dimensional membrane-only molec ularly thick in one dimension but relatively infinite in the other two; a structure composed of small molecules, not covalently linked, and therefore, infinitely mobile and variable, but yet a structure with great stability and one largely impermeable to most biomolecules. These membrane-forming properties are shared by many amphipathic polar lipids-phospholipids, glycolipids, and sphingolipids-that differ significantly from each other in the nature of their polar head groups and their fatty acids. These variations in structure allow a range of specific interactions among membrane lipids and between lipids and proteins and also provide for membranes of variable, but controlled, fluidity. In this way, phospholipids provide an appropriate milieu for functional membrane proteins and also significantly modulate their catalytic activities.
Download or read book Analysis of Sterols and Other Biologically Significant Steroids written by W. David Nes and published by Elsevier. This book was released on 2012-12-02 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analysis of Sterols and Other Biologically Significant Steroids provides the fundamental training for the analysis of selected sterols and steroids. The book is composed of chapters that review the spectroscopic and chromatographic properties of certain sterols and steroids. The text also teaches how to isolate and characterize sterols and steroid metabolites of plant, fungal, and insect origin. Lipoprotein analysis and the utilization of physical-analytical techniques are likewise provided. Biochemists, microbiologists, and medical physiologists will find the book useful.
Download or read book Steroids and their Medicinal Potential written by Abid Hussain Banday and published by Bentham Science Publishers. This book was released on 2023-07-18 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Steroids are an important class of biomolecules with diverse roles and functions. Besides being important as structural and signaling molecules, these molecules hold promise against numerous disorders, including cardiovascular conditions, cancer, inflammation, and autoimmune disorders. Many investigations have demonstrated that steroidal frameworks may provide lipid solubility, receptor selectivity, or membrane binding properties to non-steroidal pharmacophores. However, the therapeutic use of steroids can be dangerous when they're used incorrectly. Additionally, misconceptions about steroids among athletes or recreational users can lead to steroid abuse and poor health outcomes. Steroids and their MedicinalPotential discusses the classification, distribution, biosynthesis, chemical synthesis, and semi-synthesis of different steroids. The medicinal potential of each class is exhaustively discussed in different chapters. The latest advances and developments in steroid-based drug discovery are also discussed thoroughly. The book aims to address general questions and concerns about steroids, providing readers with a useful resource on the subject. Key Features -Provides comprehensive coverage on all aspects of steroids including steroid chemistry, biochemistry, medicinal potential, drug discovery, and advances in target-binding interactions of steroid-based drugs -Includes chapters dedicated to anabolic steroids and their abuse -Designed as an accessible source of information for understanding steroidal drugs with structured chapters-Includes references for advanced readers.
Download or read book Analysis of Sterols written by J. Goad and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge is of two kinds. We know a subject ourselves, or we know where we can find information on it Samuel Johnson, 18 April, 1775* Sterols are among the most studied groups of natural products with interest commencing in the 19th century and running to the present. Investigations have embraced the refinement of separation procedures, the development of new analytical techniques and instrumentation for structure elucidation, the unravelling of biosynthetic mechanisms, the determination of the physiological functions of sterols, and the role they play in health and disease. In the past 20-30 years interest in the medical implications of sterol biochemistry, studies on the sterols of plants, algae and fungi, and the identification of the many unusual sterols from marine organisms have proceeded in parallel and somewhat independently. Although the motiva tion and goals for the various lines of investigation have differed widely the researchers working in each of these areas have contributed a wealth of knowledge to the literature relating to the analysis of sterols and many diverse new sterols have been discovered. We conceived this book as a modest attempt to bring together some of this literature in the hope that it may be helpful to newcomers to sterol research. We had originally intended to produce a 'handbook' outlining in detail the protocols to be followed for sterol extraction, chromatography, NMR analysis, etc. in order to identify the components of a sterol mixture.
Download or read book Terpenoids and Steroids written by K H Overton and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 90 years The Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.
Download or read book Terpenoids and steroids a review of the literature published between 5 1975 written by [Anonymus AC02048143] and published by Royal Society of Chemistry. This book was released on 1975 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of the Newer Techniques of Analysis written by Ivor L. Simmons and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a group of selected papers given at the November, 1972, meeting of the Eastern Analytical Symposium. As has always been the intent of the series, "Progress in Analytical Chemistry," the papers are written by authorities who are also active workers in their fields. Included are applications of Raman Spectroscopy, X-Ray Diffraction, Emission Spectroscopy, Nu clear Magnetic Resonance, Liquid Chromatography, Thin-Layer Chromatography, Pyrolysis Gas Chromatography, Mass Spectrometry, Gas Chromatography and the powerful marriage of the last two. In modern analysis there is a constant search for applications of existing instrumentation in new ways to produce results hitherto unobtainable. In every case both the versatility of the researcher and the flexibility of the method used are amply illustrated. Thanks are due to the authors of the papers for their efforts in coping with the many problems of producing a manuscript in finished form enabling rapid publication. Ivor L. Simmons Galen W. Ewing v CONTENTS Raman Spectroscopy for the Study of Nematic Liquid Crystals . . . . . . 1 Bernard J. Bulkin Recent Developments in Arc Emission Spectroscopy: Introductory Remarks . . . . . . . . . . 13 Ramon M. Barnes Advances in the Static-Argon, DC-ARC Spectrochemical Source . . • . . 23 W. A. Gordon, K. M. Hambidge, and M. L. Franklin The Use of Calculated X-ray Powder Patterns in the Interpretation of Quantitative Analysis 45 C. Clark, D. K. Smith, and G. G. Johnson, Jr.
Download or read book U S Environmental Protection Agency Library System Book Catalog written by United States. Environmental Protection Agency. Library Systems Branch and published by . This book was released on 1975 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:
