Download or read book Modeling the Kinetics of Bacterial Horizontal Gene Transfer in Low nutrient Aquatic Environments written by Chad Crain and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The study presented in this dissertation focuses on characterizing the rates of horizontal gene transfer (HGT) by conjugation in Gram-negative bacteria under nutrient-limiting conditions. The HGT rates and frequencies of different Gram-negative mating pairs were evaluated and quantified experimentally. Escherichia coli S17-1 was used as a donor strain, while recipient bacteria were Acinetobacter calcoaceticus and E. coli DH5[alpha], Cupriavidus necator JMP134, Pseudomonas aeruginosa PAO1, Burkholderia vietnamiensis G4. A broad-host-range green fluorescence protein (GFP) reporter system, using plasmids pMMB190 and pRK-Km (a pRK415 derivative) was constructed for visual detection and quantification of HGT events under different conditions.The primary data generated in the various mating experiments were used as empirical input data to run a batch HGT kinetics model. The developed mathematical model was derived from a system of mass action rate expressions to predict the increase in transconjugant species under surface and planktonic mating conditions. The model is driven largely by unique intrinsic rate coefficients of gene transfer (k(z)) that were determined for different mating pairs. The nutrient-limiting conditions used in the experiments allowed for gene transfer kinetics to be observed and quantified, largely in the absence of growth, so that gene transfer would not be overestimated. The results of this study indicate that gene transfer varies considerably between donor and recipient mating pairs, and is markedly enhanced in surface-associated incubations ranging from 5.56 x 104 to 3.70 x 102 transconjugants per minute, compared to 2.8 x 102 to 1.56 x 101 per minute under planktonic conditions. The kinetics of gene transfer in most mating pairs appeared to lag at earlier time points ( 3 hours), but increased markedly after 8 hours for surface incubations. Isogenic mating pairs (e.g. E. coli) demonstrated much higher rates of gene transfer under both incubation conditions, despite possessing the slowest growth rates compared to non-isogenic mating pairs (e.g. E. coli with Cupriavidus necator JMP134, Pseudomonas aeruginosa PAO1 or Burkholderia vietnamiensis G4).The results of the various modeling simulations suggest that gene transfer kinetics using the developed mass-action-based model is sufficient for predicting the rate of gene transfer of a bulk mating population for different species when accounting for growth. The accuracy is driven by unique intrinsic rate terms (k(z)) that are expressed for individual mating pairs. Variability of k(z) terms was seen in short mating incubations, but these terms converged to uniform values at later times ( 6 hours). The uniform k(z) values provide accurate predictions of transconjugant increases during simulations, as confirmed by primary experimental data. The combination of k(z) terms along with transport equations can be reduced to a simplified two-equation model for evaluating the distribution and transport of transconjugant species in subsurface aquatic environments. Moreover, transport dynamics of mating pair species can be further characterized using confocal microscopy to generate real-time datasets. Based on the comparison between primary confocal data and simulations from the two-equation model, the Happel sphere-in-cell model is not predictive of species transport and distribution at pore-scale levels, because the model is far too idealized. Based on visual data generated with the HGT reporter system, single-cell gene transfer can be detected and quantified in real time under static and dynamic conditions and evaluated under nutrient-limiting conditions to confirm model predictions.
Download or read book Aquatic Microbial Communities written by John Cairns and published by . This book was released on 1977 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Individual Microbe Single Cell Analysis and Agent Based Modelling written by Johan H. J. Leveau and published by Frontiers Media SA. This book was released on 2019-02-19 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent technological advances in single-cell microbiology, using flow cytometry, microfluidics, x-ray fluorescence microprobes, and single-cell -omics, allow for the observation of individuals within populations. Simultaneously, individual-based models (or more generally agent-based models) allow for individual microbes to be simulated. Bridging these techniques forms the foundation of individual-based ecology of microbes (µIBE). µIBE has elucidated genetic and phenotypic heterogeneity that has important consequences for a number of human interests, including antibiotic or biocide resistance, the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the potential of pathogens to cause disease. Individual-based models can help us to understand how these sets of traits of individual microbes influence the above. This eBook compiles all publications from a recent Research Topic in Frontiers in Microbiology. It features recent research where individual observational and/or modelling techniques are applied to gain unique insights into the ecology of microorganisms. The Research Topic “The Individual Microbe: Single-Cell Analysis and Agent-Based Modelling” arose from the 2016 @ASM conference of the same name hosted by the American Society for Microbiology at its headquarters in Washington, D.C. We are grateful to ASM for funding and hosting this conference.
Download or read book Microbes and Microbial Technology written by Iqbal Ahmad and published by Springer Science & Business Media. This book was released on 2011-02-01 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on successful application of microbial biotechnology in areas such as medicine, agriculture, environment and human health.
Download or read book Horizontal Gene Transfer written by Maria Boekels Gogarten and published by Humana Press. This book was released on 2009-03-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Horizontal gene transfer (HGT) events encompass processes as varied as the exchange of genetic material between microbes coexisting in the same environment, between symbiotic bacteria and their eukaryotic hosts, and the evolution of organelles by symbiosis, in which whole genomes are acquired. In Horizontal Gene Transfer: Genomes in Flux, expert researchers contribute an overview of HGT concepts as well as specific case histories that highlight the most current progress to inspire future work. Divided into three sections, the volume begins with an overview of terminology, concepts and the implications of HGT on current evolutionary thought and philosophy, and continues with methods involving computer and bioinformatics analyses of genomic data as well as molecular biology techniques for identifying, quantifying, and differentiating instances of HGT. A section of case studies follows, which provides detailed accounts of how HGT has shaped evolution across the diversity of organisms and organismal lineages. As a volume of the highly successful Methods in Molecular BiologyTM series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Cutting-edge and thoroughly detailed, Horizontal Gene Transfer: Genomes in Flux examines how HGT has contributed to genome evolution and how understanding HGT impacts our ability to accurately reconstruct and comprehend the web-like evolutionary history in order to aid scientists in furthering their own research.
Download or read book Freshwater Microbiology written by David C. Sigee and published by John Wiley & Sons. This book was released on 2005-09-27 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique textbook takes a broad look at the rapidly expanding field of freshwater microbiology. Concentrating on the interactions between viruses, bacteria, algae, fungi and micro-invertebrates, the book gives a wide biological appeal. Alongside conventional aspects such as phytoplankton characterisation, seasonal changes and nutrient cycles, the title focuses on the dynamic and applied aspects that are not covered within the current textbooks in the field. Complete coverage of all fresh water biota from viruses to invertebrates Unique focus on microbial interactions including coverage of biofilms, important communities on all exposed rivers and lakes. New information on molecular and microscopical techniques including a study of gene exchange between bacteria in the freshwater environment. Unique emphasis on the applied aspects of freshwater microbiology with particular emphasis on biodegradation and the causes and remediation of eutrophication and algal blooms.
Download or read book Rates Constants and Kinetics Formulations in Surface Water Quality Modeling written by Environmental Research Laboratory (Athens, Ga.) and published by . This book was released on 1978 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Hydrocarbon and Lipid Microbiology written by Terry McGenity and published by Springer. This book was released on 2010-03-01 with total page 4699 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook provides a comprehensive overview of microbial interactions with the major forms of hydrocarbons, oils, and lipids in or entering the biosphere. It is the definitive resource on the physiological mechanisms and adaptive strategies characteristic of the microbial lifestyle that plays out at hydrophobic material: aqueous liquid interfaces.
Download or read book The Root Canal Biofilm written by Luis E. Chávez de Paz and published by Springer. This book was released on 2015-10-20 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the current state of research on the basic scientific aspects of root canal biofilm biology within a clinically applicable context. Root canal biofilms are complex polymicrobial structures adhering to the root canal surface that are formed by microorganisms invading the pulpal space of teeth, and are associated with persistent root canal infections. Concerted efforts to study root canal biofilms have been made in the past decade, resulting in the publication of observational and experimental studies that detail the morphology and biology of these structures in infected root canals. In addition to confirming that bacteria in root canals do not exist in free-floating planktonic states as previously assumed, this new information on root canal biofilm infections has provided an opportunity to re-evaluate conventional clinical protocols and improve endodontic therapeutic measures.
Download or read book Bacteriophages written by David R. Harper and published by Springer Nature. This book was released on 2021-01-30 with total page 1376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first major reference work dedicated to the mannifold industrial and medical applications of bacteriophages provides both theoretical and practical insights into the emerging field of bacteriophage biotechnology. The book introduces to bacteriophage biology, ecology and history and reviews the latest technologies and tools in bacteriophage detection, strain optimization and nanotechnology. Usage of bacteriophages in food safety, agriculture, and different therapeutic areas is discussed in detail. This book serves as essential guide for researchers in applied microbiology, biotechnology and medicine coming from both academia and industry.
Download or read book Biofilms in Wastewater Treatment written by Stefan Wuertz and published by IWA Publishing. This book was released on 2003-04-30 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: The central theme of the book is the flow of information from experimental approaches in biofilm research to simulation and modeling of complex wastewater systems. Probably the greatest challenge in wastewater research lies in using the methods and the results obtained in one scientific discipline to design intelligent experiments in other disciplines, and eventually to improve the knowledge base the practitioner needs to run wastewater treatment plants. The purpose of Biofilms in Wastewater Treatment is to provide engineers with the knowledge needed to apply the new insights gained by researchers. The authors provide an authoritative insight into the function of biofilms on a technical and on a lab-scale, cover some of the exciting new basic microbiological and wastewater engineering research involving molecular biology techniques and microscopy, and discuss recent attempts to predict the development of biofilms. This book is divided into 3 sections: Modeling and Simulation; Architecture, Population Structure and Function; and From Fundamentals to Practical Application, which all start with a scientific question. Individual chapters attempt to answer the question and present different angles of looking at problems. In addition there is an extensive glossary to familiarize the non-expert with unfamiliar terminology used by microbiologists and computational scientists. The colour plate section of this book can be downloaded by clicking here. (PDF Format 1 MB)
Download or read book Applied and Environmental Microbiology written by and published by . This book was released on 1998 with total page 1034 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Processes in Microbial Ecology written by David L. Kirchman and published by OUP Oxford. This book was released on 2012-02-02 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial ecology is the study of interactions among microbes in natural environments and their roles in biogeochemical cycles, food web dynamics, and the evolution of life. Microbes are the most numerous organisms in the biosphere and mediate many critical reactions in elemental cycles and biogeochemical reactions. Because microbes are essential players in the carbon cycle and related processes, microbial ecology is a vital science for understanding the role of the biosphere in global warming and the response of natural ecosystems to climate change. This novel textbook discusses the major processes carried out by viruses, bacteria, fungi, protozoa and other protists - the microbes - in freshwater, marine, and terrestrial ecosystems. It focuses on biogeochemical processes, starting with primary production and the initial fixation of carbon into cellular biomass, before exploring how that carbon is degraded in both oxygen-rich (oxic) and oxygen-deficient (anoxic) environments. These biogeochemical processes are affected by ecological interactions, including competition for limiting nutrients, viral lysis, and predation by various protists in soils and aquatic habitats. The book neatly connects processes occurring at the micron scale to events happening at the global scale, including the carbon cycle and its connection to climate change issues. A final chapter is devoted to symbiosis and other relationships between microbes and larger organisms. Microbes have huge impacts not only on biogeochemical cycles, but also on the ecology and evolution of more complex forms of life, including Homo sapiens..
Download or read book Molecular Microbial Ecology Manual written by Antoon D. L. Akkermans and published by Springer. This book was released on 2014-01-13 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.
Download or read book Marine Cyanobacteria written by Loïc Charpy and published by Musee Oceanographique. This book was released on 1999 with total page 644 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Desk Encyclopedia of Microbiology written by Moselio Schaechter and published by Academic Press. This book was released on 2010-04-19 with total page 1277 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Desk Encyclopedia of Microbiology, Second Edition is a single-volume comprehensive guide to microbiology for the advanced reader. Derived from the six volume e-only Encyclopedia of Microbiology, Third Edition, it bridges the gap between introductory texts and specialized reviews. Covering topics ranging from the basic science of microbiology to the current "hot" topics in the field, it will be invaluable for obtaining background information on a broad range of microbiological topics, preparing lectures and preparing grant applications and reports. The most comprehensive single-volume source providing an overview of microbiology to non-specialists Bridges the gap between introductory texts and specialized reviews Provides concise and general overviews of important topics within the field making it a helpful resource when preparing for lectures, writing reports, or drafting grant applications
Download or read book Biogeochemistry of Wetlands written by K. Ramesh Reddy and published by CRC Press. This book was released on 2022-09-10 with total page 926 pages. Available in PDF, EPUB and Kindle. Book excerpt: The globally important nature of wetland ecosystems has led to their increased protection and restoration as well as their use in engineered systems. Underpinning the beneficial functions of wetlands are a unique suite of physical, chemical, and biological processes that regulate elemental cycling in soils and the water column. This book provides an in-depth coverage of these wetland biogeochemical processes related to the cycling of macroelements including carbon, nitrogen, phosphorus, and sulfur, secondary and trace elements, and toxic organic compounds. In this synthesis, the authors combine more than 100 years of experience studying wetlands and biogeochemistry to look inside the black box of elemental transformations in wetland ecosystems. This new edition is updated throughout to include more topics and provide an integrated view of the coupled nature of biogeochemical cycles in wetland systems. The influence of the elemental cycles is discussed at a range of scales in the context of environmental change including climate, sea level rise, and water quality. Frequent examples of key methods and major case studies are also included to help the reader extend the basic theories for application in their own system. Some of the major topics discussed are: Flooded soil and sediment characteristics Aerobic-anaerobic interfaces Redox chemistry in flooded soil and sediment systems Anaerobic microbial metabolism Plant adaptations to reducing conditions Regulators of organic matter decomposition and accretion Major nutrient sources and sinks Greenhouse gas production and emission Elemental flux processes Remediation of contaminated soils and sediments Coupled C-N-P-S processes Consequences of environmental change in wetlands# The book provides the foundation for a basic understanding of key biogeochemical processes and its applications to solve real world problems. It is detailed, but also assists the reader with box inserts, artfully designed diagrams, and summary tables all supported by numerous current references. This book is an excellent resource for senior undergraduates and graduate students studying ecosystem biogeochemistry with a focus in wetlands and aquatic systems.
