Download or read book Microinjection written by Chengyu Liu and published by Humana. This book was released on 2019-12-10 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.
Download or read book Microinjection written by Juan Carlos Lacal and published by Birkhäuser. This book was released on 2013-12-01 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.
Download or read book Microinjection and Transgenesis written by Angel Cid-Arregui and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 619 pages. Available in PDF, EPUB and Kindle. Book excerpt: The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.
Download or read book Biotechnology Applications of Microinjection Microscopic Imaging and Fluorescence written by Peter H. Bach and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Individual cells behave in surpnsmg ways that cannot be deduced from the averaged results of an organ as assessed by the use of conventional biochemical methods. Thus multicellular plant and animals systems are being investigated by an increasing array of histochemical and cytochemical techniques based on general chemical or specific immunological interactions to identify structural materials and to assess biological activities. In recent years there has been an increasing range of fluorescent probes, along with advanced computerised imaging and analysis techniques, which allows the behaviour of individual living cells to be followed in considerable detail. The parallel use of microinjection, microelectrodes and patch-clamping provides additional information about cells and their responses. Recombinant DNA technology has highlighted the desirability and the power of microinjecting defined materials into specific cells and so manipulating their fundamental biochemistry. New hypotheses are being tested which will form the cornerstone of future developments across the whole spectrum of biotechnology. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21st-24th April, 1992 with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological exploration. A novel feature of the meeting was paiticipation by instrument manufacturers in the programme: there were hands-on workshops (where living cells could be examined), combined with the poster sessions.
Download or read book Micro Injection Molding written by Guido Tosello and published by Carl Hanser Verlag GmbH Co KG. This book was released on 2018-08-06 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Micro Injection Molding" meets the need for a dedicated book dealing exclusively with micro injection molding and overcoming the challenges of managing and processing polymer materials at ultra-small scales. Micro injection molding is the primary process for the mass production of polymer components with critical dimensions in the sub-millimeter range; however, it is not just a simple downscaling of conventional injection molding, and specific material-process-product interactions must be understood in order to achieve near zero-defect net-shape micro molded products. Micro molding is typically associated with ultra-high accuracy and superior process capabilities. Micro molded products have dimensional tolerances down to the single-digit micrometer range and surface finish with roughness from the sub-micrometer down to a few nanometers range. Micro and nano-structured tool surfaces are reproduced with very high replication fidelity onto the polymer products. Micro injection molding is highly suitable for the manufacture of multifunctional micro components such as micro implants, microfluidic systems, polymer micro optical elements, and micro mechanical systems. This book provides engineers, project managers, researchers, consultants, and other professionals involved in precision polymer processing and micro manufacturing with a comprehensive, up-to-date, and detailed treatment of the main topics related to micro molding, from material and process technology to tooling, to key-enabling technologies, and multimaterial process variations. Contents: • Part 1 – Polymer Materials and Process Micro Technology: micro injection molding machines technology; micro molding process monitoring and control; polymer materials structure and properties in micro injection molding parts; surface replication in micro injection molding • Part 2 – Tooling Technologies for Micro Mold Making: micro machining technologies for micro injection mold making; ultra-precision machining technologies for micro injection mold making; surface treatment of mold tools in micro injection molding • Part 3 – Micro Molding Key-Enabling Technologies: vacuum-assisted micro injection molding; modeling and simulation of micro injection molding; metrological quality assurance in micro injection molding; additive manufacturing for micro tooling and micro part rapid prototyping • Part 4 – Multimaterial Micro Processing: micro powder injection molding; multimaterial micro injection molding
Download or read book Microinjection and Organelle Transplantation Techniques written by Julio E. Celis and published by . This book was released on 1986 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microinjection of tissue culture cells using glass; Transformation capacity of SV40 DNA and subgenomic DNA fragments; Modulation of cellular activities via microinjection into living cells; Studies of retroviral packagin;. Erythrocyte-mediated transfer: methods; Erythrocyte-mediated transfer: applications; Liposomes for microinjection of molecules into cells; Liposomes in drug targeting; Liposomes as carriers for gene transfer in vivo; Transformation of mammalian cells with DNA using the calcium technique; The somatic cell as a tes tube: comparison between various transfer techniques.
Download or read book Transgenic Animal Technology written by Carl A. Pinkert and published by Newnes. This book was released on 2014-06-17 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Download or read book Microinjection written by David J. Carroll and published by Humana Press. This book was released on 2016-08-23 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
Download or read book Microinjection written by Chengyu Liu and published by . This book was released on 2018 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Intermediate filament dynamics in living cells as analyzed by microinjection of tagged vimentin written by Karen Lynn Vikstrom and published by . This book was released on 1990 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Pronuclear Microinjection of Recombinase A Protein coated Single and Double stranded Rat Stearoyl CoA Desaturase Construct DNA and Gene Integration Efficiency in the Mouse written by Jeffrey-Gevork Najarian and published by . This book was released on 2001 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Medaka written by Masato Kinoshita and published by John Wiley & Sons. This book was released on 2009-09-15 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medaka: Biology, Management, and Experimental Protocols, written by experienced researchers and reviewed by international leaders in the medaka field will provide details on how to set up and maintain medaka colonies in animal facilities, how to troubleshoot systems, how to handle the fish when applied to experimental methods, and most importantly it will introduce the researcher to cutting edge research in basic and applied biology using medaka as a model animal. The book will include well-written descriptions of experimental methods and protocols designed to educate the reader how to understand and handle medaka effectively. Medaka: Biology, Management, and Experimental Protocols will serve as the definitive reference on the species providing essential information on medaka biology, genetics, and genomics, practical guidance to maintenance of fish stocks, and valuable experimental protocols all in a single volume. This book will be a must have addition to the library of fish researchers and those using medaka as a model organism within their laboratories.
Download or read book Electrophysiology and Microinjection written by P. Michael Conn and published by Elsevier. This book was released on 2016-07-02 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrical activity is central to the functioning and nature of neural cells. Every effort has been made in this volume to provide representative examples of useful techniques that have made this area a productive component of the neurosciences. Convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Cell recording: from conscious and freely behaving animals, measurement of calcium currents, and multiple electrodes**Voltage clamping and voltammetry: apparatus and applications**Specific ion channels and receptors: expression in oocytes, single-cell secretion, purification of calcium channel drug receptors**Special electrodes and other equipment: movable electrodes, microelectrodes, multiple electrodes, en passant recording, microincubators for optical studies**Special preparations: multicompartment cell cultures, grease-gap methods, perforated path techniques, giant proteoliposomes for reconstitution studies, microinjection**Data analysis and reduction: digital filtering on a personal computer, filtering on a personal computer, fitting of single-channel dwell time distributions, discrimination of kinetic models, voltage noise analysis, list-oriented analysis of single-channel systems
Download or read book Molecular Methods in Developmental Biology written by Matt Guille and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.
Download or read book Rabbit Biotechnology written by Louis-Marie Houdebine and published by Springer Science & Business Media. This book was released on 2009-04-24 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: Louis-Marie Houdebine and Jianglin Fan The study of biological functions of proteins and their possible roles in the pathogenesis of human diseases requires more and more relevant animal m- els. Although mice including genetically modified mice offer many possibilities, other non-murine species are absolutely required in some circumstances. Rabbit is one of these species, which has been widely used in biomedical studies. This animal is genetically and physiologically closer to humans including cardiov- cular system and metabolism characteristics. Rabbit is thus more appropriate than mice to study some diseases such as atherosclerosis and lipid metabolism. Because of its larger size, surgery manipulation, bleeding, and turn-over studies are much easier performed in rabbits than in mice. Furthermore, transgenic rabbits can be produced using microinjection and other methods such as lentiviral v- tors. Cloning in rabbits has been proved possible, even though still laborious and time-consuming. Hopefully, functional rabbit ES cell lines will be available in the coming years. Gene deletion or knock-out in rabbits will then become possible.
Download or read book Micromanipulation in Assisted Conception written by Steven D. Fleming and published by Cambridge University Press. This book was released on 2003-11-06 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This guide to micromanipulation techniques, for assisted conception in a clinical setting, includes detailed descriptions of all common micromanipulation systems currently in use in IVF laboratories. In explaining how to optimize their successful use, the volume covers state-of-the-art techniques including ICSI, and procedures such as assisted hatching and the blastomere biopsy (for PGD). Valuable information on troubleshooting mechanical and technical difficulties is provided to help professionals ranging from technicians to consultant obstetricians master the techniques.
Download or read book Microinjection written by J. C. Lacal and published by . This book was released on 1996-10-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
