Download or read book Microfluidic Processes to Create Structured Microparticle Arrangements and Their Applications written by Jae Jung Kim (Ph. D.) and published by . This book was released on 2017 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multifunctional polymeric microparticles have shown the great potentials in a variety of fields. While the advance in particle synthesis allows for fine tuning of their physical properties and chemical functionality, particle manipulation is still appealing, but challenging issue in colloidal science. In order to expand the utility of microparticles, many particle manipulation techniques have been developed to arrange large-scale of particles at precise locations. However, current approaches cannot simultaneously fulfill desired capabilities of arrangement: scalability, precision, specificity, and versatility. This thesis explores the ability to synthesize particles with a controllability of characteristics, and development of a new microfluidic platform, porous microwell arrays, to create structured large-scale microparticle arrays using a scaling theory, which is a function of particles' characteristics. Lastly, we demonstrate the potential of generated particle arrays in various bioengineering application and material sciences. First, we synthesize anisotropic, cell-adhesive microparticles using stop flow lithography (SFL) and carbodimide coupling. Synthesized microparticles are functionalized with collagen or poly-L-lysine using streptavidin-biotin interaction, resulting in cell-adhesiveness. After functionalization, target cells are spread on the particles and spatially patterned only on the functionalized region. Thus, cells are not exposed to potentially harmful components of particle synthesis processes, photoinitiators and ultraviolet light, ensuring no physiological changes. Second, we synthesize multi-striped, upconverting nanocrystal (UCN)-laden microparticles using SFL. Distinct upconversion emission colors are combined with the ability to spatial pattern them, providing superior encoding capacities. We can fine-tune upconversion emission by controlling the dopant composition in nanocrystal, and synthesize microparticles in a highly reproducible manner by SFL, allowing for the development of predictable decoding system. Two types of particles are synthesized with this appealing encoding strategy for two distinct applications: thermally stable particles for anti-counterfeiting application; and porous hydrogels for multiplexed microRNA detection. Third, we develop a microfluidic platform, porous microwell arrays, to manipulate microparticles while fulfilling all four desired capabilities (i.e. scalability, precision, specificity, and versatility). Microwells are fabricated on top of porous membrane by a vacuum-assisted molding method. Particles are guided and assembled into wells by hydrodynamic force associated with fluid flow through pores in microwell. Iteration of assembly and washing steps ensures high-throughput, large-scale particle arrangement with high yields on filling and capturing. Scaling theory allows for the rational design of platform to specifically position microparticles depending on their physical characteristics (i.e. size, shape, and modulus), enabling to generate complex patterns. We utilize this platform in three practical applications: high-throughput, large-scale single-cell arrays; microenvironment fabrication for neutrophil chemotaxis; and UCN-laden covert 2D tags for anti-counterfeiting. Lastly, we modified the porous microwell platform to a closed system, microfluidic channels, to park and isolate particles in monodisperse droplets surrounded by fluorinated oil. Rational modification retains the platform's desired capabilities, resulting in a single particle in a droplet with high yields on both parking and isolation. Particle-in-droplet arrays enables the observation of reaction in confined volume over the time. Such arrays can be utilized to accumulate the desired product from enzymatic reaction, amplifying the signal and improving the sensitivity of bioassays. We demonstrate the highly sensitive, multiplexed miRNA detections with these particle-in-droplet arrays.

Download or read book Microfluidics for Biological Applications written by Wei-Cheng Tian and published by Springer Science & Business Media. This book was released on 2009-03-02 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics for Biological Applications provides researchers and scientists in the biotechnology, pharmaceutical, and life science industries with an introduction to the basics of microfluidics and also discusses how to link these technologies to various biological applications at the industrial and academic level. Readers will gain insight into a wide variety of biological applications for microfluidics. The material presented here is divided into four parts, Part I gives perspective on the history and development of microfluidic technologies, Part II presents overviews on how microfluidic systems have been used to study and manipulate specific classes of components, Part III focuses on specific biological applications of microfluidics: biodefense, diagnostics, high throughput screening, and tissue engineering and finally Part IV concludes with a discussion of emerging trends in the microfluidics field and the current challenges to the growth and continuing success of the field.

Download or read book Microencapsulation in the Food Industry written by Robert Sobel and published by Academic Press. This book was released on 2022-09-27 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microencapsulation in the Food Industry: A Practical Implementation Guide, Second Edition continues to focus on the development of new microencapsulation techniques for researchers and scientists in the field. This practical reference combines the knowledge of new and novel processing techniques, materials and selection, regulatory aspects and testing and evaluation of materials. It provides application specific uses of microencapsulation as it applies to the food and nutraceutical industries. This reference offers unique solutions to some very specific product needs in the field of encapsulation. This second edition highlights changes in the industry as a result of a field that has traversed from the micro scale level to nano-scaled encapsulation and includes two new chapters, one on regulatory, quality, process scale-up, packaging, and economics and the other on testing and quality control. - Includes new characterization methodologies to understand chemical and physical properties for functionality of the final microencapsulated material - Presents the latest research and developments in the area of nano-scale encapsulation and intelligent packaging - Provides new testing tools to assess products containing microencapsulated actives

Download or read book Microfluidic Synthesis and Properties of Bio inspired Colloids written by Harry Zijian An and published by . This book was released on 2014 with total page 119 pages. Available in PDF, EPUB and Kindle. Book excerpt: Today, custom-tailored polymeric hydrogel microparticles are routinely used for drug delivery, medical diagnostics, as well as many fundamental studies in colloidal science. There is growing consensus that physical attributes, such as size, shape, internal structure, and mechanical deformability, of the particles can influence material performance, especially when they are placed into a biological setting. Over the past two decades, a host of particle fabrication techniques have been proposed, which are able to produce microgels with various physical properties, though maintaining independent control over them remains to be difficult. This thesis explores the synthesis of a new class of functional microparticles bearing the likeness of mammalian cells through the use of a new microfluidics-based lithography process, and demonstrates the utility of these biomimetic microparticles in novel biomedical applications. First, we devise a microscope projection technique based on stop-flow lithography (SFL), which allows free-standing microparticles of any arbitrary 2D-extruded, mask-defined shape to be patterned down to the cellular size regime (>/= 10 [mu]m) from UV-crosslinkable oligomer formulations in a semi-continuous fashion. By modulating the degree of oxygen inhibition during synthesis, we achieve previously unattainable particle sizes. Brownian diffusion of colloidal discs in bulk suggests the out-of-plane dimension can be as small as 0.8 [mu]m, which agrees with confocal microscopy measurements. We measure the hindered diffusion of microdiscs near a solid interface and compared our results to theoretical predictions. These biocompatible colloidal particles can also flow through physiological microvascular networks formed by endothelial cells undergoing vasculogensis under minimal hydrostatic pressure. Second, inspired by the hierarchical structure of eukaryotic cells, we synthesize composite microparticles containing a homogenous distribution of hydrophobic compartments from crosslinkable silicone oil-in-water nanoemulsions. The nanoemulsion loadings achieved in our composite microgels have, to our knowledge, among the highest ever demonstrated in a hydrogel material. In addition, we perform proof-of-concept assays to show several orthogonal motifs, such as tunable hydrophobic anchoring at the oil/water interface and matrix degradation at high pH, by which both hydrophobic and hydrophilic compounds, including small molecules, proteins and the nanoemulsion droplets themselves, can be effectively encapsulated in and released from the resulting microparticles over a wide range of timescales. And last, we fabricate red blood cell-mimicking, oxygen-carrying composite microparticles from perfluorocalin-in-water nanoemulsions. Unlike silicone oil nanoemulsions, the PFD-based formulations require osmotic stabilization due to a finite solubility of oil in the aqueous continuous phase. The presence of perfluorocarbon oil droplets increases the solubility and diffusivity of oxygen in the prepolymer solution, thereby enhancing the rate of O2 inhibition during microparticle synthesis. We develop a simple model that successfully predicts the augmented O2 mass transport, which agrees well with experimental data. Encapsulating nanodroplets in the hydrogel network allows us to generate and preserve small droplets under nearly surfactant-free conditions via quick washing steps post-synthesis.

Download or read book Artificial Cilia written by Jaap MJ den Toonder and published by Royal Society of Chemistry. This book was released on 2013-05-31 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cilia are tiny hairs covering biological cells to generate and sense fluid flow. Millions of years of evolution have inspired a novel technology which is barely a decade old. Artificial cilia have been developed to control and sense fluid flow in microscopic systems, presenting new and interesting options for flow control in lab-on-a-chip devices. This appealing link between nature and technology has seen rapid development in the last few years, and this book presents a review of the state-of-the-art in the form of a professional reference book. The editors have pioneered the field, having initiated a major European project on this topic soon after its inception. Active researchers in academia and industry will benefit from the comprehensive nature of this book, while postgraduates and those new to the field will gain a clear understanding of the theory, techniques and applications of artificial cilia.

Download or read book Microfluidics and Nanofluidics written by Mohsen Sheikholeslami Kandelousi and published by BoD – Books on Demand. This book was released on 2018-08-22 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the present book, various applications of microfluidics and nanofluidics are introduced. Microfluidics and nanofluidics span a broad array of disciplines including mechanical, materials, and electrical engineering, surface science, chemistry, physics and biology. Also, this book deals with transport and interactions of colloidal particles and biomolecules in microchannels, which have great importance to many microfluidic applications, such as drug delivery in life science, microchannel heat exchangers in electronic cooling, and food processing industry. Furthermore, this book focuses on a detailed description of the thermal transport behavior, challenges and implications that involve the development and use of HTFs under the influence of atomistic-scale structures and industrial applications.

Download or read book Functional Materials for Bio Applications written by Laura Maria Vergani and published by Frontiers Media SA. This book was released on 2020-12-15 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Download or read book Introduction to Microfluidics written by Patrick Tabeling and published by . This book was released on 2010-05-06 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics deals with fluids flowing in miniaturized systems, and has practical applications in the pharmaceutical, biomedical and chemical engineering fields. This text provides an introduction to this emerging discipline.

Download or read book Synthetic Biodegradable Polymer Scaffolds written by Anthony Atala and published by Springer Science & Business Media. This book was released on 1997-12-01 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This body of work represents the first volume of a book series covering the field of tissue engineering. Tissue engineering, which refers to a category of therapeutic or diagnostic products and processes which are based upon a combination of living cells and biomaterials, was defined as a field only a few years ago (1988). Tissue engineering is an inherently interdisciplinary field, combining bioengineering, life sciences and clinical sciences. The definition of this area of work as the field of tissue engineering brought together scientists from multiple backgrounds who already were working toward the achievement of similar goals. Why a book series exclusively devoted to tissue engineering? The field of tissue engineering is heterogeneous. The cells involved in tissue engineering can be autologous, allogeneic or xenogeneic. The biomaterials utilized can be either naturally occurring, synthetic or a combination of both. The appli cation of the technology can be either for acute or permanent purposes. An attempt to cover the field of tissue engineering in a single volume, with the degree of detail necessary for individuals with different scientific back grounds and disciplines, would be a difficult task to accomplish, particularly when this field is just emerging and changing rapidly. Therefore, addressing different technologies within the field of tissue engineering, in a comprehen sive manner, is the main mission of this series of volumes. A stellar group of scientists has been brought together to form the editorial board of the series.

Download or read book Cell Microencapsulation written by Emmanuel C. Opara and published by Humana. This book was released on 2016-10-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in cell microencapsulation, the challenges to the technology, and the current status of its application in different clinical situations. This book is divided in five sections: Section I is an introductory part that discusses historical developments of the technology and its current challenges, as well as the various applications of cell microencapsulation; Section II discusses the main approaches and devices currently used in cell microencapsulation; Section III presents an overview of the various polymeric materials currently in use for cell microencapsulation and the enabling technologies to either monitor or enhance encapsulated cell function; Section IV gives specific examples of the methods used to encapsulate various cell types; and Section V provides an overview of the different clinical situations in which cell microencapsulation has been applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Cell Microencapsulation: Methods and Protocols is a valuable reference for researchers, engineers, clinicians, and other healthcare professionals, as well as food technologists who will find detailed descriptions of methods for the microencapsulation of specific cell types and their current of potential clinical and industrial applications. This volume also includes detailed information about the design and manufacture of different devices including large-scale production devices for use in cell microencapsulation.

Download or read book Advances in Microfluidic Technologies for Energy and Environmental Applications written by Yong Ren and published by BoD – Books on Demand. This book was released on 2020-05-20 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics have aroused a new surge of interest in recent years in environmental and energy areas, and inspired novel applications to tackle the worldwide challenges for sustainable development. This book aims to present readers with a valuable compendium of significant advances in applying the multidisciplinary microfluidic technologies to address energy and environmental problems in a plethora of areas such as environmental monitoring and detection, new nanofluid application in traditional mechanical manufacturing processes, development of novel biosensors, and thermal management. This book will provide a new perspective to the understanding of the ever-growing importance of microfluidics.

Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun James Li and published by Woodhead Publishing. This book was released on 2013-10-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries.

Download or read book Microfluidics written by Bastian E. Rapp and published by Elsevier. This book was released on 2022-10-07 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics: Modeling, Mechanics and Mathematics, Second Edition provides a practical, lab-based approach to nano- and microfluidics, including a wealth of practical techniques, protocols and experiments ready to be put into practice in both research and industrial settings. This practical approach is ideally suited to researchers and R&D staff in industry. Additionally, the interdisciplinary approach to the science of nano- and microfluidics enables readers from a range of different academic disciplines to broaden their understanding. Alongside traditional fluid/transport topics, the book contains a wealth of coverage of materials and manufacturing techniques, chemical modification/surface functionalization, biochemical analysis, and the biosensors involved. This fully updated new edition also includes new sections on viscous flows and centrifugal microfluidics, expanding the types of platforms covered to include centrifugal, capillary and electro kinetic platforms. - Provides a practical guide to the successful design and implementation of nano- and microfluidic processes (e.g., biosensing) and equipment (e.g., biosensors, such as diabetes blood glucose sensors) - Provides techniques, experiments and protocols that are ready to be put to use in the lab, or in an academic or industry setting - Presents a collection of 3D-CAD and image files on a companion website

Download or read book Digital Holographic Methods written by Stephan Stuerwald and published by Springer. This book was released on 2018-10-12 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents not only the simultaneous combination of optical methods based on holographic principles for marker-free imaging, real-time trapping, identification and tracking of micro objects, but also the application of substantial low coherent light sources and non-diffractive beams. It first provides an overview of digital holographic microscopy (DHM) and holographic optical tweezers as well as non-diffracting beam types for minimal-invasive, real-time and marker-free imaging as well as manipulation of micro and nano objects. It then investigates the design concepts for the optical layout of holographic optical tweezers (HOTs) and their optimization using optical simulations and experimental methods. In a further part, the book characterizes the corresponding system modules that allow the addition of HOTs to commercial microscopes with regard to stability and diffraction efficiency. Further, based on experiments and microfluidic applications, it demonstrates the functionality of the combined setup, and discusses several types of non-diffracting beams and their application in optical manipulation. The book shows that holographic optical tweezers, including several non-diffracting beam types like Mathieu beams, combined parabolic and Airy beams, not only open up the possibility of generating efficient multiple dynamic traps for micro and nano particles with forces in the pico and nano newton range, but also the opportunity to exert optical torque with special beams like Bessel beams, which can facilitate the movement and rotation of particles by generating microfluidic flows. The last part discusses the potential use of a slightly modified DHM-HOT-system to explore the functionality of direct laser writing based on a two photon absorption process in a negative photoresist with a continuous wave laser

Download or read book 3D Printed Microfluidic Devices written by Savas Tasoglu and published by MDPI. This book was released on 2019-01-10 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "3D Printed Microfluidic Devices" that was published in Micromachines

Download or read book Microfluidics Based Microsystems written by S. Kakaç and published by Springer. This book was released on 2010-06-30 with total page 621 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains an archival record of the NATO Advanced Study Institute on Microfluidics Based Microsystems – Fundamentals and App- cations held in Çe ?me-Izmir, Turkey, August 23–September 4, 2009. ASIs are intended to be high-level teaching activity in scientific and technical areas of current concern. In this volume, the reader may find interesting chapters and various microsystems fundamentals and applications. As the world becomes increasingly concerned with terrorism, early - spot detection of terrorist’s weapons, particularly bio-weapons agents such as bacteria and viruses are extremely important. NATO Public Diplomacy division, Science for Peace and Security section support research, Advanced Study Institutes and workshops related to security. Keeping this policy of NATO in mind, we made such a proposal on Microsystems for security. We are very happy that leading experts agreed to come and lecture in this important NATO ASI. We will see many examples that will show us Microfluidics usefulness for rapid diagnostics following a bioterrorism attack. For the applications in national security and anti-terrorism, microfluidic system technology must meet the challenges. To develop microsystems for security and to provide a comprehensive state-of-the-art assessment of the existing research and applications by treating the subject in considerable depth through lectures from eminent professionals in the field, through discussions and panel sessions are very beneficial for young scientists in the field.

Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1598 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.