Download or read book Microbial Source Tracking of Bacteroides to Identify Human and Bovine Fecal Loading in McDaniel Lake written by Kalie M. Somerville and published by . This book was released on 2015 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fecal waste enters waterways from run-off during rain events, and is especially high in agricultural areas due to manure application and livestock. Increased fecal waste may also be due to direct human influence. This fecal loading may lead to eutrophication and poses health hazards to humans. By determining the source of fecal pollution, practices can be implemented to reduce the amount entering waterways. Bacteroides sp. have previously shown high host specificity allows for species-specific identification of fecal sources. Using quantitative real-time PCR (qPCR), human-associated and bovine-associated Bacteroides DNA, as well as total Bacteroides DNA can be quantified and concentrations of fecal contamination in lakes and streams can be determined. Amplification control plasmids containing 16S rDNA from human and bovine specific Bacteroides were constructed using TOPO cloning. Samples taken from McDaniel Lake in Springfield, MO Dec. 2013-Dec. 2014 were analyzed with end-point PCR and fecal concentrations determined with qPCR. Quantitative PCR showed increased sensitivity and selectivity, compared to end-point PCR. While Bacteroides sp. were present in all samples, less than 1% of total Bacteroides in McDaniel Lake was identified as human- or bovine-associated Bacteroides for the sample period. The use of qPCR is shown to be a valuable tool in identifying and quantifying fecal inputs and monitoring nonpoint source pollution as urban and agricultural areas encroach on natural systems.

Download or read book Microbial Source Tracking written by Michèle Gourmelon and published by Frontiers Media SA. This book was released on 2022-01-18 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Host Specific Microbial Source Tracking and Quantification Using a Partial 16SrRNA Sequence of Bacteroides on the Finley River a Tributary of the James River in Southwest Missouri written by Neil J. Van Asch and published by . This book was released on 2009 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial source tracking (MST) is a general term given to any process which aims to determine the source of microbial contamination found in an environmental (usually water) sample. These methods include both phenotypic and genotypic strategies. In this study, quantitative PCR was used to evaluate water samples for species specific (cow and human) Bacteroides in the Finley River. The Finley River is susceptible to impacts from both bovine and humans as it flows through farmland and urban areas. Water samples were collected from sites identified in a previous study that had high E. coli levels. Two samples were collected at each site (One sample for enumeration of E. coli and the other to assay for Bacteroides). Sampling was done approximately three times per month for one year. For each sampling event, pH, DO, turbidity, water temperature, flow rate, and rainfall were recorded. The results showed lower than expected cow markers throughout the sampling period; while human markers were much more prevalent than cow markers during the drier (and warmer) months. A correlation was found between human specific Bacteroides markers and E. coli at one site, but not at either of the other two sites. This method affords more informative surveillance data to area health departments so more appropriate remediation strategies can be developed.

Download or read book Microbial Source Tracking of Bacteroidales and Human Virus Monitoring in Calleguas Creek Watershed written by Beverly Jo Kildare and published by . This book was released on 2005 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Using Microbial Source Tracking to Identify Fecal Contamination Sources in Lake Montauk on Long Island New York written by Tristen N. Tagliaferri and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Discrimination of Human and Non human Fecal Sources with Rapid Methods in Coastal Waters and Sediments written by Amity Gayle Zimmer-Faust and published by . This book was released on 2015 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial contamination in coastal waters is an important public health and economic problem worldwide. It is common for the sources as well as the environmental fate and transport of fecal contamination in recreational waters to be unknown making it difficult to mitigate the input and to understand the relative health risk associated with a given water body. A comprehensive understanding of sources and bacterial dynamics is needed for effective mitigation and management of microbial contaminants. The main objectives of this study were to identify sources of fecal contamination to a chronically impaired Southern California watershed, to demonstrate applicability of microbial source tracking (MST) tools, including source-specific markers for discrimination of human and non-human sources, to evaluate the fate of fecal contaminates in coastal sediments, and to develop and optimize immunomagnetic separation/adenosine triphosphate (IMS/ATP) assays for rapid enumeration of viable fecal contamination. A three-year MST study was conducted to help explain elevated levels of surfzone fecal indicator bacteria (FIB) at Topanga State Beach, a critically impaired Southern California Beach. This study investigated sources of FIB to the Topanga watershed and the applicability of using MST technology longer time scales. MST markers effectively elucidated temporal and seasonal trends in fecal bacterial levels, and dog and gull marker appeared to be a significant sources to Topanga lagoon and Topanga State Beach. However, a lack of correlation between FIB and marker measurements was noted, and dog marker and FIB levels did not covary when compared at different Southern California beaches. Sediments were found to play an important and variable role in environmental fate of MST markers and FIB. Variable decay was observed for different indicators and in different sediments, with differences noted even within one watershed. The human HF183 marker was useful for providing evidence of recent inputs of human fecal contamination and behaved similarly to the molecular marker for Campylobacter (qCAMP) and FIB in brackish sediments. The general Bacteroides (GB3) and enterococci (ENT1A) markers were more conservative and under certain circumstances had comparable decay to culturable FIB. Application of a suite of markers may be necessary for effective evaluation of sediment fecal bacterial levels. Moreover, differences were observed between relative decay amongst the different sediments tested, illustrating the need for more routine sediment monitoring. IMS/ATP assays provided useful information regarding fecal contamination levels and measurements made had a consistent relationship with measurements made by standard methods. IMS/ATP utilizes paramagnetic beds and target-specific antibodies to isolate target organisms. Following isolation, adenosine tri-phosphate (ATP) is extracted from the target population and quantified. The Cov-IMS/ATP method rapidly measured viable enterococci in complex surface waters, providing a useful eld tool for assessment of coastal water quality and for identi cation of hot spots of fecal contamination. An inversely-coupled (Inv-IMS/ATP) assay for detection of Bacteroides thetaiotaomicron was developed and applied for rapid detection of human-associated fecal contamination. The Inv-IMS/ATP assay yielded measurements of viable B. thetaiotaomicron that were comparable to the HF183 human marker in complex source waters impacted with both wastewater and runoff, and the Inv-IMS/ATP assay was able to effectively differentiate between surface waters impacted with adequately and inadequately treated wastewater. IMS/ATP assays show promise for rapid evaluation of recreational water quality in areas where access to more expensive methods is limited and in areas where water quality is unpredictable. This research highlights the difficulties and complexities associated with effective tracking and management of microbial contaminates in the coastal environment. Additional research evaluating relative aging of molecular markers and relative contributions from different sources is needed to fully interpret field-based source marker data. Sediments were shown to have an important and variable role in fate of fecal contaminants in the environment. Additional studies are needed evaluating how watershed models can most effectively be adapted to include a sediment compartment and how different sources of fecal contamination and source markers decay in sediments with variable characteristics. IMS/ATP assays showed promise and can be successfully applied in complex waters for rapid enumeration of viable fecal contamination; additional verification of assay performance is needed at complex sites impacted with multiple sources.

Download or read book Microbial Source Tracking Methods Applications and Case Studies written by Charles Hagedorn and published by Springer. This book was released on 2011-06-09 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the origin of fecal pollution is essential in assessing potential health risks as well as for determining the actions necessary to remediate the quality of waters contaminated by fecal matter. As a result, microbial source tracking (MST) has emerged as a field that has evolved and diversified rapidly since the first approaches were described only a decade ago. In response to the emergence of MST, there have been three large multi-laboratory method comparison studies (two in the US and one in Europe), plus numerous workshops, book chapters, and review articles dedicated to synthesizing information on the topic. Furthermore, a federal (USEPA) guide document describing the uses and limitations of MST methods was published in 2005, and a book dedicated to MST as an emerging issue in food safety was published in 2007. These documents provide a collective body of literature on MST that is both conflicting and complementary, often repetitious, and difficult to condense and interpret. In addition, it does not reflect the current diversity of MST approaches with different organisms, newer methodologies such as quantitative PCR, and anthropogenic chemicals, nor does it embrace the scope of MST research being conducted around the world. The three editors of the book, all with extensive MST expertise, have developed chapters and invited authors who reflect the rich diversity and truly international scope of MST. The unifying theme throughout the book is the design of more standardized approaches to MST that include performance criteria (regardless of method or organism), plus recommendations for field study design and MST implementation. The editors intend that this book will serve as a valuable reference for all those who are involved with

Download or read book Animal Waste Water Quality and Human Health written by A. Dufour and published by IWA Publishing. This book was released on 2012-10-14 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: Domestic animals contaminate recreational waters and drinking-water sources with excreta and pathogens; but this threat to public health is inadequately understood and is insufficiently addressed in regulations. More than 85% of the world’s faecal wastes is from domestic animals such as poultry, cattle, sheep and pigs. These animals harbor zoonotic pathogens that are transported in the environment by water, especially runoff. However little information exists on health effects associated with exposure to this potential hazard to human health; and water standards focused on control of human fecal contamination do reflect the contribution of non-human fecal contamination to risk. Does compliance with current monitoring practices using microbial indicators provide protection against animal and bird sources of fecal contamination? Prepared with contributions from a group of international experts, Animal Waste, Water Quality and Human Health considers microbial contamination from domestic animal and bird sources and explores the health hazards associated with this microbial contamination and approaches to protecting public health. Animal Waste, Water Quality and Human Health will be of interest to regulators with responsibility for recreational waters, drinking water quality and water reuse; policymakers working in water quality, public health and agriculture; decision makers responsible for livestock management; and scientists and practitioners concerned with many affected subjects. Topics covered include: Credible waterborne zoonotic pathogens are discussed and ranked according to their potential hazard level. Each pathogen is described with regard to their sources, reservoirs, and infectivity. Faecal production rates of various domestic animals are discussed, alongside pathogen transmission in animal populations, pathogen prevalence in animals and “supershedders”. Transport of fecal indicator organisms and their episodic occurrence in catchments. Interventions for improving food safety and reducing production losses. The impact of interventions, e.g. enhanced attenuation and storage to prevent spills; benchmarking against best management practices to reduce diffuse source contamination. Models to inform design of farm-scale best management practices and the effectiveness of best management practices for attenuating pathogen transport within catchments. The complex nature of human exposure to zoonotic waterborne pathogens; including the relationships among livestock waste contamination, water impairment, zoonotic pathogens, and human infection and illness. Human exposure interventions include case studies that discuss eradicating disease in discharging populations, adding filtration to minimal treated water to reduce Cryptosporidium occurrence and UV disinfection of beach waters to reduce beach postings. Indicators, sanitary surveys and source attribution techniques; risk assessment of exposure to zoonotic pathogens, including an interactive risk comparison approach. A review of epidemiological studies that address the relationship between swimmer illness and exposure to waters contaminated by nonhuman fecal wastes. Economic evaluation of the costs and benefits associated with animal waste management and human health.

Download or read book Impacts of Climate Change on Human Health in the United States written by US Global Change Research Program and published by Simon and Schuster. This book was released on 2018-02-06 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: As global climate change proliferates, so too do the health risks associated with the changing world around us. Called for in the President’s Climate Action Plan and put together by experts from eight different Federal agencies, The Impacts of Climate Change on Human Health: A Scientific Assessment is a comprehensive report on these evolving health risks, including: Temperature-related death and illness Air quality deterioration Impacts of extreme events on human health Vector-borne diseases Climate impacts on water-related Illness Food safety, nutrition, and distribution Mental health and well-being This report summarizes scientific data in a concise and accessible fashion for the general public, providing executive summaries, key takeaways, and full-color diagrams and charts. Learn what health risks face you and your family as a result of global climate change and start preparing now with The Impacts of Climate Change on Human Health.

Download or read book Waterborne Zoonoses written by World Health Organization and published by World Health Organization. This book was released on 2004 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zoonoses are infectious diseases that can be transmitted from animals (both wild and domestic) to humans. A significant number of emerging and re-emerging waterborne zoonotic pathogens have been recognised over recent decades, such as SARS, E. coli, campylobacter and cryptosporidium. This publication assesses current knowledge about waterborne zoonoses and identifies strategies and research needs for anticipating and controlling future emerging water-related diseases, in order to better protect the health of both humans and animals. It is based on the discussions of a workshop held in the United States in September 2003, which included 29 experts from 14 countries and diverse disciplines including microbiology, water epidemiology, medicine, sanitary engineering, food safety and regulatory policy.

Download or read book Microbial Threats to Health written by Institute of Medicine and published by National Academies Press. This book was released on 2003-08-25 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: Infectious diseases are a global hazard that puts every nation and every person at risk. The recent SARS outbreak is a prime example. Knowing neither geographic nor political borders, often arriving silently and lethally, microbial pathogens constitute a grave threat to the health of humans. Indeed, a majority of countries recently identified the spread of infectious disease as the greatest global problem they confront. Throughout history, humans have struggled to control both the causes and consequences of infectious diseases and we will continue to do so into the foreseeable future. Following up on a high-profile 1992 report from the Institute of Medicine, Microbial Threats to Health examines the current state of knowledge and policy pertaining to emerging and re-emerging infectious diseases from around the globe. It examines the spectrum of microbial threats, factors in disease emergence, and the ultimate capacity of the United States to meet the challenges posed by microbial threats to human health. From the impact of war or technology on disease emergence to the development of enhanced disease surveillance and vaccine strategies, Microbial Threats to Health contains valuable information for researchers, students, health care providers, policymakers, public health officials. and the interested public.

Download or read book Endospore forming Soil Bacteria written by Niall A. Logan and published by Springer Science & Business Media. This book was released on 2011-07-06 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aerobic endospore-forming bacteria are found in soils of all kinds, ranging from acid to alkaline, hot to cold, and fertile to desert. It is well known that endospores confer special properties upon their owners and play dominant parts in their life cycles and dispersal, and much has been written about the spores, genetics, and economic importance of these organisms. Much has also been written about soil ecology, but there is a relative dearth of literature that brings together different aspects of the behaviour and characters of endospore-formers with their contributions to soil ecosystems. This Soil Biology volume fills that gap. Following chapters that describe the current classification of these organisms, that review methods for their detection and for studying their life cycles in soils, and that examine their dispersal, other chapters show that they are active and dynamic members of soil floras that interact widely with other soil inhabitants, with roles in nitrogen fixation, denitrification, and soil remediation.

Download or read book New Bacterial Vaccines written by Ronald W. Ellis and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Bacterial Vaccines focuses upon unfulfilled needs for bacterial vaccines. The increase in drug resistance among many bacterial species has increased the need for new bacterial vaccines. This book serves as a comprehensive reference on the major aspects of developing new bacterial vaccines. The distinctive feature of this book is that it focuses upon new vaccines now under development by reviewing key issues for each vaccine target and new technologies being applied to developing new vaccines. This book should prove useful for students in the life sciences, scientists, developers of vaccines and biotechnology products, clinicians, regulators, and health-care practitioners.

Download or read book Biogenesis of Fatty Acids Lipids and Membranes written by Otto Geiger and published by Springer. This book was released on 2019-01-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes).All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes.

Download or read book Habitats and Biota of the Gulf of Mexico Before the Deepwater Horizon Oil Spill written by C. Herb Ward and published by Springer. This book was released on 2017-06-26 with total page 948 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY-NC 2.5 license. The Gulf of Mexico is an open and dynamic marine ecosystem rich in natural resources but heavily impacted by human activities, including agricultural, industrial, commercial and coastal development. The Gulf of Mexico has been continuously exposed to petroleum hydrocarbons for millions of years from natural oil and gas seeps on the sea floor, and more recently from oil drilling and production activities located in the water near and far from shore. Major accidental oil spills in the Gulf are infrequent; two of the most significant include the Ixtoc I blowout in the Bay of Campeche in 1979 and the Deepwater Horizon Oil Spill in 2010. Unfortunately, baseline assessments of the status of habitats and biota in the Gulf of Mexico before these spills either were not available, or the data had not been systematically compiled in a way that would help scientists assess the potential short-term and long-term effects of such events. This 2-volume series compiles and summarizes thousands of data sets showing the status of habitats and biota in the Gulf of Mexico before the Deepwater Horizon Oil Spill. Volume 2 covers historical data on commercial and recreational fisheries, with an analysis of marketing trends and drivers; ecology, populations and risks to birds, sea turtles and marine mammals in the Gulf; and diseases and mortalities of fish and other animals that inhabit the Gulf of Mexico.

Download or read book Drug Abuse Handbook written by Jozef Bicerano and published by CRC Press. This book was released on 2006-12-21 with total page 1286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following the well-received first edition, the Drug Abuse Handbook, Second Edition is a thorough compendium of the knowledge of the pharmacological, medical, and legal aspects of drugs. The book examines criminalistics, pathology, pharmacokinetics, neurochemistry, treatment, as well as drugs and drug testing in the workplace and in sports, and the

Download or read book Biohacker s Handbook written by Olli Sovijärvi and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: