Download or read book Microbial Community Analysis of PH 4 Thermal Springs in Yellowstone National Park written by Xiaoben Jiang and published by . This book was released on 2017 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt: The pH of the majority of thermal springs in Yellowstone National Park (YNP) is from 1 to 3 and 6 to 10; relatively few springs (~5%) have a pH range of 4?5. We used 16S rRNA gene pyrosequencing to investigate microbial communities sampled from four pH 4 thermal springs collected from four regions of YNP that differed in their fluid temperature and geochemistry. Our results revealed that the composition of bacterial communities varied among the sites, despite sharing similar pH values. The taxonomic composition and metabolic functional potential of the site with the lowest temperature (55 °C), a thermal spring from the Seven Mile Hole (SMH) area, were further investigated using shotgun metagenome sequencing. The taxonomic classification, based on 372 Mbp of unassembled metagenomic reads, indicated that this community included a high proportion of Chloroflexi, Bacteroidetes, Proteobacteria, and Firmicutes. Functional comparison with other YNP thermal spring metagenomes indicated that the SMH metagenome was enriched in genes related to energy production and conversion, transcription, and carbohydrate transport. Analysis of genes involved in nitrogen metabolism revealed assimilatory and dissimilatory nitrate reduction pathways, whereas the majority of genes involved in sulfur metabolism were related to the reduction of sulfate to adenylylsulfate, sulfite, and H2S. Given that pH 4 thermal springs are relatively less common in YNP and thermal areas worldwide, they may harbor novel microbiota and the communities that inhabit them deserve further investigation.

Download or read book Thermophilic Microorganisms written by Fu-li Li and published by Caister Academic Press Limited. This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thermophilic microorganisms thrive in a variety of marine and terrestrial habitats. These organisms have evolved several biochemical and molecular strategies to counteract the deleterious effects of the high temperatures in their environments. In this book, leading scientists highlight the current progress in the most topical areas of research providing a timely overview of the field. The authors discuss current technical challenges and future development trends.--

Download or read book Microbes of Yellowstone National Park written by Thermal Biology Institute and published by . This book was released on 20?? with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbiomes of Extreme Environments written by Ajar Nath Yadav and published by CRC Press. This book was released on 2021-03-21 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: The extreme microbiomes are those microorganisms thriving under extreme conditions where no other living being will have any chance to survive. The extreme habitats are those presenting high temperatures (thermophiles), low temperature (psychrophiles), hypersaline environments (halophiles), low and high pH (Acidophiles/alkaliphiles), high pressure (Piezophiles) are distributed worldwide. The extreme habitats have proved to offer a unique reservoir of genetic diversity and biological source of extremophiles. The extremophilic microbial diversity and their biotechnological potential use in agricultural and industrial applications will be a milestone for future needs. Extremophiles and their cell components, therefore, are expected to play an important role in the chemical, food, pharmaceutical, paper and textile industries as well as environmental biotechnology.

Download or read book Systems biology and ecology of microbial mat communities written by Martin G. Klotz and published by Frontiers Media SA. This book was released on 2016-04-11 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial mat communities consist of dense populations of microorganisms embedded in exopolymers and/or biomineralized solid phases, and are often found in mm-cm thick assemblages, which can be stratified due to environmental gradients such as light, oxygen or sulfide. Microbial mat communities are commonly observed under extreme environmental conditions, deriving energy primarily from light and/or reduced chemicals to drive autotrophic fixation of carbon dioxide. Microbial mat ecosystems are regarded as living analogues of primordial systems on Earth, and they often form perennial structures with conspicuous stratifications of microbial populations that can be studied in situ under stable conditions for many years. Consequently, microbial mat communities are ideal natural laboratories and represent excellent model systems for studying microbial community structure and function, microbial dynamics and interactions, and discovery of new microorganisms with novel metabolic pathways potentially useful in future industrial and/or medical applications. Due to their relative simplicity and organization, microbial mat communities are often excellent testing grounds for new technologies in microbiology including micro-sensor analysis, stable isotope methodology and modern genomics. Integrative studies of microbial mat communities that combine modern biogeochemical and molecular biological methods with traditional microbiology, macro-ecological approaches, and community network modeling will provide new and detailed insights regarding the systems biology of microbial mats and the complex interplay among individual populations and their physicochemical environment. These processes ultimately control the biogeochemical cycling of energy and/or nutrients in microbial systems. Similarities in microbial community function across different types of communities from highly disparate environments may provide a deeper basis for understanding microbial community dynamics and the ecological role of specific microbial populations. Approaches and concepts developed in highly-constrained, relatively stable natural communities may also provide insights useful for studying and understanding more complex microbial communities.

Download or read book Microbial Evolution under Extreme Conditions written by Corien Bakermans and published by Walter de Gruyter GmbH & Co KG. This book was released on 2015-03-10 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Today's microorganisms represent the vast majority of biodiversity on Earth and have survived nearly 4 billion years of evolutionary change. However, we still know little about the processes of evolution as applied to microorganisms and microbial populations. Microbial evolution occurred and continues to take place in a vast variety of environmental conditions that range from anoxic to oxic, from hot to cold, from free-living to symbiotic, etc. Some of these physicochemical conditions are considered "extreme", particularly when inhabitants are limited to microorganisms. It is easy to imagine that microbial life in extreme environments is somehow more constrained and perhaps subjected to different evolutionary pressures. But what do we actually know about microbial evolution under extreme conditions and how can we apply that knowledge to other conditions? Appealingly, extreme environments with their relatively limited numbers of inhabitants can serve as good model systems for the study of evolutionary processes. A look at the microbial inhabitants of today's extreme environments provides a snapshot in time of evolution and adaptation to extreme conditions. These adaptations manifest at different levels from established communities and species to genome content and changes in specific genes that result in altered function or gene expression. But as a recent (2011) report from the American Academy of Microbiology observes: "A complex issue in the study of microbial evolution is unraveling the process of evolution from that of adaptation. In many cases, microbes have the capacity to adapt to various environmental changes by changing gene expression or community composition as opposed to having to evolve entirely new capabilities." We have learned much about how microbes are adapted to extreme conditions but relatively little is known about these adaptations evolved. How did the different processes of evolution such as mutation, immigration, horizontal (lateral) gene transfer, recombination, hybridization, genetic drift, fixation, positive and negative selection, and selective screens contribute to the evolution of these genes, genomes, microbial species, communities, and functions? What are typical rates of these processes? How prevalent are each of these processes under different conditions? This book explores the current state of knowledge about microbial evolution under extreme conditions and addresses the following questions: What is known about the processes of microbial evolution (mechanisms, rates, etc.) under extreme conditions? Can this knowledge be applied to other systems and what is the broader relevance? What remains unknown and requires future research? These questions will be addressed from several perspectives including different extreme environments, specific organisms, and specific evolutionary processes.

Download or read book Bar coded Pyrosequencing Reveals Shared Bacterial Community Properties Along the Temperature Gradients of Two Alkaline Hot Springs in Yellowstone National Park written by Scott R. Miller and published by . This book was released on 2009 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: An understanding of how communities are organized is a fundamental goal of ecology but one which has historically been elusive for microbial systems. We used a bar-coded pyrosequencing approach targeting the V3 region of the bacterial small-subunit rRNA gene to address the factors that structure communities along the thermal gradients of two alkaline hot springs in the Lower Geyser Basin of Yellowstone National Park. The filtered data set included a total of nearly 34,000 sequences from 39 environmental samples. Each was assigned to one of 391 operational taxonomic units (OTUs) identified by their unique V3 sequence signatures. Although the two hot springs differed in their OTU compositions, community resemblance and diversity changed with strikingly similar dynamics along the two outflow channels. Two lines of evidence suggest that these community properties are controlled primarily by environmental temperature. First, community resemblance decayed exponentially with increasing differences in temperature between samples but was only weakly correlated with physical distance. Second, diversity decreased with increasing temperature at the same rate along both gradients but was uncorrelated with other measured environmental variables. This study also provides novel insights into the nature of the ecological interactions among important taxa in these communities. A strong negative association was observed between cyanobacteria and the Chloroflexi, which together accounted for 70% of the sequences sampled. This pattern contradicts the longstanding hypothesis that coadapted lineages of these bacteria maintain tightly cooccurring distributions along these gradients as a result of a producer-consumer relationship. We propose that they instead compete for some limiting resource(s). --Abstract.

Download or read book Recreational Water Illnesses written by Erica Leoni and published by MDPI. This book was released on 2019-02-05 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Recreational Water Illnesses" that was published in IJERPH

Download or read book Thermophilic Microorganisms and Life at High Temperatures written by T.D. Brock and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: From 1965 through 1975, I conducted an extensive field and laboratory research project on thermophilic microorganisms. The field work was based primarily in Yellowstone National Park, using a field laboratory we set up in the city of W. Yellowstone, Montana. The laboratory work was carried out from 1965 through 1971 at Indiana University, Bloomington, and subsequently at the University of Wisconsin, Madison. Although this research project began small, it quickly ramified in a wide variety of directions. The major thrust was an attempt to understand the ecology and evolutionary relationships of thermophilic microorganisms, but research also was done on biochemical, physiologic, and taxonomic aspects of thermophiles. Four new genera of thermophilic microorganisms have been discovered during the course of this 10-year period, three in my laboratory. In addition, a large amount of new information has been obtained on some thermophilic microorganisms that previously had been known. In later years, a considerable amount of work was done on Yellowstone algal bacterial mats as models for Precambrian stromatolites. In the broadest sense, the work could be considered geomicrobiological, or biogeochemi cal, and despite the extensive laboratory research carried out, the work was always firmly rooted in an attempt to understand thermophilic microorga nisms in their natural environments. Indeed, one of the prime motivations for initiating this work was a view that extreme environments would provide useful models for studying the ecology of microorganisms. As a result of this 10-year research project, I published over 100 papers.

Download or read book Extremophiles in Eurasian Ecosystems Ecology Diversity and Applications written by Dilfuza Egamberdieva and published by Springer. This book was released on 2018-07-26 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores various aspects of thermophilic and halophilic microbes from Eurasian ecosystems, which have proved to offer a unique reservoir of genetic diversity and biological source of extremophiles. It also covers the biotechnological uses of extremophiles, and their potential use in agricultural and industrial applications. The topics addressed include but are not limited to: diversity and microbial ecology, microbe-environment interactions, adaptation and evolution, element cycling and biotechnological applications of thermophiles and halophiles in Eurasian ecosystems. In order to review the progress made in biology and biotechnological applications of thermophiles and halophiles, the book combines review papers and results of original research from various specialists and authorities in the field. It includes several chapters describing the microbial diversity and ecology of geothermal springs distributed among the territory of various Eurasian countries, such as Armenia, Bulgaria, China, Georgia, India, Italy, Pakistan and Turkey. A dedicated chapter discusses selected aspects of thermophilic chemolithotrophic bacteria isolated from mining sites (sulfide ores); detailed descriptions of various thermophile microbes isolated from high-temperature environments and their biotechnological potential are also provided. Subsequent chapters describe the diversity and ecology of halophilic microbes harbored in saline and hypersaline lakes in Iran, Turkey and China; soil and plant microbiomes in saline arid lands of Uzbekistan; microbial diversity in Asian deserts; and the potential applications of thermophilic and halophilic microbes as exopolysaccharide (EPS) producers, focusing on the chemistry and applications of the EPS they produce. We hope that this book will prove valuable as an up-to-date overview of the current state of research on Eurasian extremophiles in general and thermophiles and halophiles in particular. Many questions remain unanswered, and we hope that it will stimulate further studies in this intriguing and promising field.

Download or read book Characterization of a Yellowstone Hot Spring Microbial Community by 5S RRNA Sequences written by David Allan Stahl and published by . This book was released on 1985 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt: The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to better characterized ones were established. Quantitation of the total 5S-sized rRNAs revealed a complex microbial community of three dominant members, a predominant archaebacterium affiliated with the sulfur-metabolizing (dependent) branch of the archaebacteria, and two eubacteria distantly related to Thermus spp. The archaebacterial and the eubacterial 5S rRNAs each constituted about half the examined population.

Download or read book The microbial ferrous wheel iron cycling in terrestrial freshwater and marine environments written by David Emerson and published by Frontiers E-books. This book was released on with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past 15 years, there has been steady growth in work relating to the microbial iron cycle. It is now well established that in anaerobic environments coupling of organic matter utilization to Fe reduction is a major pathway for anaerobic respiration. In iron-rich circumneutral environments that exist at oxic-anoxic boundaries, significant progress has been made in demonstrating that unique groups of microbes can grow either aerobically or anaerobically using Fe as a primary energy source. Likewise, in high iron acidic environments, progress has been made in the study of communities of microbes that oxidize iron, and in understanding the details of how certain of these organisms gain energy from Fe-oxidation. On the iron scarcity side, it is now appreciated that in large areas of the open ocean Fe is a key limiting nutrient; thus, a great deal of research is going into understanding the strategies microbial cells, principally phytoplankton, use to acquire iron, and how the iron cycle may impact other nutrient cycles. Finally, due to its abundance, iron has played an important role in the evolution of Earth’s primary biogeochemical cycles through time. The aim of this Research Topic is to gather contributions from scientists working in diverse disciplines who have common interests in iron cycling at the process level, and at the organismal level, both from the perspective of Fe as an energy source, or as a limiting nutrient for primary productivity in the ocean. The range of disciplines may include: geomicrobiologists, microbial ecologists, microbial physiologists, biological oceanographers, and biogeochemists. Articles can be original research, techniques, reviews, or synthesis papers. An overarching goal is to demonstrate the environmental breadth of the iron cycle, and foster understanding between different scientific communities who may not always be aware of one another’s work.

Download or read book Microbial Communities and their Interactions in the Extreme Environment written by Dilfuza Egamberdieva and published by Springer Nature. This book was released on 2021-10-19 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of the book entitled “Microbial Communities and Interactions in extreme environments” focus on thermophilic and halophilic extremophiles from various ecosystems, their biodiversity, interactions with other organisms and functions within their hostile environment. Biotechnology of extremophiles and their potential agricultural and industrial applications is the focus of this edition. However, extremophiles may cope with their challenging environments. Information on biodiversity of extremophiles and their interactions with the surrounding biomes helps in understanding their ecology and functions within their respective extreme environments. This book is of interest to teachers, researchers, microbiologists, capacity builders and policymakers. Also, the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, microbiology and environmental sciences.

Download or read book Predominant Acidilobus Like Populations from Geothermal Environments in Yellowstone National Park Exhibit Similar Metabolic Potential in Different Hypoxic Microbial Communities written by Zackary J. Jay and published by . This book was released on 2013 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-temperature (>70°C) ecosystems in Yellowstone National Park (YNP) provide an unparalleled opportunity to study chemotrophic archaea and their role in microbial community structure and function under highly constrained geochemical conditions. Acidilobus spp. (order Desulfurococcales) comprise one of the dominant phylotypes in hypoxic geothermal sulfur sediment and Fe(III)-oxide environments along with members of the Thermoproteales and Sulfolobales. Consequently, the primary goals of the current study were to analyze and compare replicate de novo sequence assemblies of Acidilobus-like populations from four different mildly acidic (pH 3.3 to 6.1) high-temperature (72°C to 82°C) environments and to identify metabolic pathways and/or protein-encoding genes that provide a detailed foundation of the potential functional role of these populations in situ. De novo assemblies of the highly similar Acidilobus-like populations (>99% 16S rRNA gene identity) represent near-complete consensus genomes based on an inventory of single-copy genes, deduced metabolic potential, and assembly statistics generated across sites. Functional analysis of coding sequences and confirmation of gene transcription by Acidilobus-like populations provide evidence that they are primarily chemoorganoheterotrophs, generating acetyl coenzyme A (acetyl-CoA) via the degradation of carbohydrates, lipids, and proteins, and auxotrophic with respect to several external vitamins, cofactors, and metabolites. No obvious pathways or protein-encoding genes responsible for the dissimilatory reduction of sulfur were identified. The presence of a formate dehydrogenase (Fdh) and other protein-encoding genes involved in mixed-acid fermentation supports the hypothesis that Acidilobus spp. function as degraders of complex organic constituents in high-temperature, mildly acidic, hypoxic geothermal systems.

Download or read book Microbial Diversity in the Genomic Era written by Surajit Das and published by Elsevier. This book was released on 2024-03-23 with total page 840 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial Diversity in the Genomic Era, Second Edition presents techniques used for microbial taxonomy and phylogeny, along with their applications and respective strengths and challenges. Though many advanced techniques for the identification of unknown bacterium are available in the genomic era, a far fewer number of the total microbial species have been discovered and identified to date. With that in mind, this book incorporates recently developed biosystematics methods and approaches to assess microbial taxonomy, with suitable recommendations for where to apply them across the range of bacterial identification and infectious disease research. Here, international researchers in the field first provide a broad overview of microbial genomics research and microbiome directed medicine, followed by sections on molecular tools for microbial diversity research, extremophilic microbial diversity, functional microbial diversity across application areas, microbial diversity and infectious disease research, and future directions for research. Step-by-step methodologies are provided for key techniques, along with applied case studies breaking down recent research studies into the practical components, illuminating pathways for new studies across the field. This new edition has been fully updated to address advances in the field of microbiome directed medicine, and whole genome sequencing for studying microbial diversity, considering both recent technological advances and new applications areas, from extremophile studies to the latest approaches in human microbiome analysis. - Instructs in techniques used for microbial taxonomy and phylogeny, with discussions of their applications and respective pros and cons - Reviews the evolving field of microbial typing and the genomic technologies that enable comparative metagenomic analysis of complex microbial environments - Covers microbiome directed translational research, as well as whole genome sequencing for studying microbial diversity, with newly added research protocols and case studies - Reviews future applications in the field of microbiome directed medicine - Features chapter contributions from global experts in the field

Download or read book Studies on Life at the Energetic Edge from Laboratory Experiments to Field Based Investigations Volume II written by Mark Alexander Lever and published by Frontiers Media SA. This book was released on 2024-01-16 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: In collaboration with Microenergy 2022: The 4th International Workshop on Microbial Life under Extreme Energy Limitation, we are proud to launch Volume II of Studies on Life at the Energetic Edge – from Laboratory Experiments to Field-Based Investigations. This workshop focuses on the energy controls on microbial life and the exploration of the biological demand for energy. Genetic adaptations and phenotypic traits that enable microorganisms to tolerate long periods of energy limitation have attracted broad scientific interest in recent years. Laboratory-based cultivation experiments have shown that the potential to survive weeks to months in the absence of energy inputs occurs across a phylogenetically wide range of microbes. Studies on natural environments have shown that energy limitation is pervasive across most habitats on Earth, from highly metabolically active surface habitats to subsurface environments that have been cut off from new energy inputs for thousands of years. Yet, much remains to be learned about the evolutionary adaptations and life history traits that enable microorganisms to live under low-energy conditions. Similarly, the spectrum of energy sources and metabolisms that enable and support life on Earth and potentially elsewhere in the Universe is far from constrained.

Download or read book Environmental Bioinorganic Chemistry of Aquatic Microbial Organisms written by Christel Hassler and published by Frontiers E-books. This book was released on 2013-07-05 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Environmental Bioinorganic Chemistry of Aquatic Microbial Organisms describes the interactions between metals and aquatic prokaryotic and eukaryotic microorganisms in their environment. Metals influence microbial growth in the aquatic environment as they can be either toxic to aquatic microbes, if present at too high concentrations in the environment, or limiting, if bio-essential and present at very low concentrations. In turn, microorganisms influence the biogeochemical cycling of metals as they affect trace metal concentrations, distributions between particulate and dissolved phase, and chemical speciation. At the sub cellular level, metalloproteins are the catalysts driving many steps in the biogeochemical cycles of major elements such as carbon, nitrogen and sulfur. Metals thus provide a link between the abundance and activity of enzymes, the growth of microorganisms, and the biogeochemical cycles of major climate influencing elements. Furthermore, the evolution of the chemistry of aquatic environments and atmosphere has left its mark on the microbial proteome as a direct result of changes in the solubility of metals. The aquatic microbial metallome thus has the potential to reveal information about key biogeochemical processes, their spatial and seasonal occurrence, and also to reveal how the geochemical environment is shaping the microbial population itself. The aim of this Research Topic is to highlight recent advances in our understanding of how metals influence the activity of aquatic microbes, and how microbes influence the biogeochemical cycling of metals. Applications of techniques in proteomics, spectroscopy, mass spectrometry and genomics are all leading to a greater understanding of the interactions between the microbial metallome and the “aquatic metallome” and thus the influence of metals on the biogeochemical cycles of climatically important elements such as carbon, nitrogen and sulfur. Both reviews and original research on the occurrence and abundance of microbial metal proteins and peptides, the utilisation of metals by aquatic microbes, the influence of microbially produced exudates on metal speciation and the biogeochemical cycling, and the toxicity of metals to microbial organisms are welcome.