Download or read book Methods of Enzymatic analysis V4 written by Hans-UIrich Bergmeyer and published by Elsevier. This book was released on 2012-12-02 with total page 761 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Enzymatic Analysis, Volume 4 reviews developments in the use of enzymes as tools in analytical biochemistry, including advances in assay techniques. It discusses the principles and methods for the elucidation of structures of enzymes, such as peptides, proteins, amino acids, fatty acid metabolites, lipids, steroids, nucleic acids, purines, pyrimidines, nucleosides, and coenzymes. It also considers the isolation and characterization of active centers in enzymes. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on proteins, peptides, and amino acids including amines and amides. Part II is concerned with fatty acid metabolites, lipids, and steroids ranging from polyunsaturated fatty acids and lecithin to choline, acetylcholine, triglycerides, glycerol, acetoacetate, triacetate, fumarylacetoacetate, 20-ketosteroids, prostaglandins, bile acids, and cholesterol. Part III discusses nucleic acids, purines, pyrimidines, nucleosides, coenzymes, and related compounds, whereas Part IV looks at other substrates and effectors such as inorganic phosphate. The book concludes with a chapter on metabolites and their concentrations in animal tissues. Biochemists as well as students and researchers working in the field of analytical biochemistry will find this book highly informative.
Download or read book Methods of Enzymatic Analysis written by Hans Ulrich Bergmeyer and published by . This book was released on 1983 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis written by Hans-UIrich Bergmeyer and published by Elsevier. This book was released on 2012-12-02 with total page 1093 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Enzymatic Analysis focuses on the general progress in enzymology and in the special field of enzymatic analysis. This book explores the commercial production of biochemical reagents for analysis and explains the transition from the possible use of enzymatic analysis to its various applications in pure and applied biochemistry. Organized into four sections, this book starts with an overview of the basis of enzymatic analysis and provides general experimental guidelines for the techniques of measurement and for the disintegration of cells and tissues. This text then provides detailed instructions for the determination of substrates and assay of enzyme activities. Other chapters explore the practical aspects and information necessary for the application of reagents to enzymatic analysis, including sources, stability, and purity required. The final section describes the commercially available enzymes, coenzymes, substrates, and several less common reagents. Biochemists, biophysicists, researchers, and graduate students will find this book extremely useful.
Download or read book Methods of Enzymatic Analysis V2 written by Hans-UIrich Bergmeyer and published by Elsevier. This book was released on 2012-12-02 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases. Part III discusses hydrolases including esterases, glycoside hydrolases, peptidases, and proteinases, whereas Part IV looks at lyases, isomerases, and ligases, such as fructose-1, 6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase. This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.
Download or read book Methods of Enzymatic Analysis Volume 4 Metabolites 1 Carbohydrates written by HU Bergmeyer (ed) and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis written by Karlfried Gawehn and published by Academic Press. This book was released on 1974 with total page 2116 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Enzymatic analysis V4 ...
Download or read book Methods of Enzymatic Analysis written by Hans Ulrich Bergmeyer and published by . This book was released on 1974 with total page 2302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis Samples reagents assessment of results written by Hans Ulrich Bergmeyer and published by . This book was released on 1983 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis Enzymes 2 Esterases glycosidases lyases ligases written by Hans Ulrich Bergmeyer and published by . This book was released on 1984 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis Methods of Enzymatic Analysis written by Hans-Ulrich Bergmeyer and published by Wiley-Blackwell. This book was released on 1985-09-02 with total page 629 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis Proteins and peptides written by Hans Ulrich Bergmeyer and published by . This book was released on 1983 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymatic Methods of Analysis written by George G. Guilbault and published by Elsevier. This book was released on 2013-10-22 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic Methods of Analysis attempts to cover all the aspects of modern enzymatic analysis. Enzymes possess a great potential usefulness in analytical chemistry. The specificity of enzymes can solve the primary problem of most analytical chemists—the analysis of one substance in the presence of many similar compounds that interfere in the analysis. The book begins with two introductory chapters devoted to general considerations of enzymes as reagents and methods of analysis of enzymatic reactions. The next four chapters deal with methods for the assay of specific enzymes, and substrate, activator, and inhibitor analysis using enzymes. In the last two chapters the immobilization of enzymes and the automation of enzymatic reactions are discussed. In addition, a listing of all commercially available enzymes is given in an appendix. It is hoped that the information presented will prove interesting and stimulating to all individuals engaged in research and development.
Download or read book Methods of Enzymatic Analysis Methods of Enzymatic Analysis written by Hans-Ulrich Bergmeyer and published by Wiley-Blackwell. This book was released on 1983-04-26 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis Metabolites 2 Tri and dicarboxylic acids purines pyrimidines and derivatives coenzymes inorganic compounds written by Hans Ulrich Bergmeyer and published by . This book was released on 1985 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Enzymatic Analysis Methods of Enzymatic Analysis written by Hans-Ulrich Bergmeyer and published by Wiley-Blackwell. This book was released on 1984-03-19 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of enzymatic analysis written by Hans Ulrich Bergmeyer and published by . This book was released on 1974 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of Enzymatic analysis V2 ...
Download or read book Enzymatic Analysis written by Janet V. Passonneau and published by Springer Science & Business Media. This book was released on 2008-02-07 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.
