Download or read book Methods in Cell Wall Cytochemistry written by K V Krishnamurthy and published by CRC Press. This book was released on 2020-07-24 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.) Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.
Download or read book Research on the Cytochemistry of Cell Walls of Microorganisms written by Farr Cytochemical Laboratories and published by . This book was released on 1964 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a part of the research program designed to solve the nutritional problems of astronauts during extended explorations of space, cell wall substances of four fungi, Heterocephalum aurantiacum, Spicaria sp., Cladosporium sp. and Linderina pennispora have been identified and localized by standard cytochemical methods. Chitin is the resistant cell wall material in the component cells of all of these fungi and results of efforts to identify cellulose in the same cell walls have been negative. Excretions which accumulate on the surfaces of the aerial corticating hyphae of H. aurantiacum are composed primarily of calcium salts and an unidentified colloidal material, yellow-orange in color and having many characteristics in common with the so-called fungal gamboge. Reactions of the previously untreated chitinous cell walls to the enzyme, chitinase, are positive. Enzymatic hydrolysis represents the most suitable method now available for the conversion of chitin to N-acetylglucosamine with the accompanying breakdown of the cell wall and concurrent release of nutritive subbussubstances i ses in the protoplast. X-rray diffraction patteettterns of chitin havevah nin have been obtained from purified cell wall samples. Measurements of these same samples, at successive stages ofoof removal of non-chitinous materials, show a gradual increase in refractive index values. (Author).
Download or read book Research on the Cytochemistry of Cell Walls of Microorganisms written by Wanda K. Farr and published by . This book was released on 1964 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a part of the research program designed to solve the nutritional problems of astronauts during extended explorations of space, cell wall substances of four fungi, Heterocephalum aurantiacum, Spicaria sp., Cladosporium sp. and Linderina pennispora have been identified and localized by standard cytochemical methods. Chitin is the resistant cell wall material in the component cells of all of these fungi and results of efforts to identify cellulose in the same cell walls have been negative. Excretions which accumulate on the surfaces of the aerial corticating hyphae of H. aurantiacum are composed primarily of calcium salts and an unidentified colloidal material, yellow-orange in color and having many characteristics in common with the so-called "fungal gamboge." Reactions of the previously untreated chitinous cell walls to the enzyme, chitinase, are positive. Enzymatic hydrolysis represents the most suitable method now available for the conversion of chitin to N-acetylglucosamine with the accompanying breakdown of the cell wall and concurrent release of nutritive substances in the protoplast. X-ray diffraction patterns of chitin have been obtained from purified cell wall samples. Measurements of these same samples, at successive stages of removal of non-chitinous materials, show a gradual increase in refractive index values. Progress has been made in the adaptation of interference microscopy to the study of chitinous cell walls during the process of hydrolysis with chitinase.
Download or read book Plant Cell Biology written by Chris Hawes and published by OUP Oxford. This book was released on 2001-05-31 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
Download or read book Methods in Cell Biology written by and published by Academic Press. This book was released on 1966-01-01 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Cell Biology
Download or read book Research on Cell Wall Cytochemistry of Selected Fungi written by Wanda K. Farr and published by . This book was released on 1965 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytochemical analyses of the four imperfect fungi, Cylindrocephalum sp., Curvularia lunata, Aspergillus oryzae, and Gliocladium deliquescens showed that the resistant material in their cell walls is chitin. All efforts to identify cellulose produced negative results. Solutions of chitinase-containing Worthington Lysozyme (Muramidase) in concentrations of 0.5mg./ml. brought about dissolution of the cell walls and dispersion of the cell contents. Enzymatic hydrolysis represents the most suitable method now available for the conversion of fungal cell wall chitin to digestible end products with the simultaneous release of nutrient substances in the protoplasm. Specific studies have continued on protoplasmic phenomena within the cells of fungi examined, especially Heterocephalum aurantiacum Plastid-like structures in the protoplasts are the cell organs which produce chitin. Chitin-forming plastids increase in number by direct division and the quantities of chitin at their surfaces, in a given cell, may be equal to or greater than the amount of chitin in the cell wall. This information necessitates the inclusion of chitin located in the protoplasts, as well as that in the cell walls, in any considerations of fungal tissues as food for astronauts in extended explorations of space. (Author).
Download or read book Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine written by Kazuo Ogawa and published by CRC Press. This book was released on 1993 with total page 828 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine provides up-to-date, comprehensive surveys and descriptions of electron microscopic cytochemical and immunocytochemical methods. It also reviews selected topics of cell biology, pathology, histology, and biomedicine. These reviews illustrate the fundamental contributions of electron microscopic cytochemistry and immunocytochemistry to biology and pathology. By bringing together methodology and the application of methods, this book presents a broad discussion of the contributions, potentials, and limitations of cytochemistry and immunocytochemistry. Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine will be a useful reference volume for cell biologists, histologists, anatomists, pathologists, physiologists, immunologists, and clinical researchers concerned with the basic mechanisms of diseases.
Download or read book Stains and Cytochemical Methods written by M.A. Hayat and published by Springer Science & Business Media. This book was released on 1993-03-31 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: The author's primary aim is to thoroughly explain the biochemical concepts governing cytochemical procedures for transmission electron microscopy. Such information provides undergraduate and graduate students, technicians, and researchers with a more profound understanding of electron micrographs, as well as the knowledge to refine existing techniques and develop new methodologies.
Download or read book Electron Microscopy and Cytochemistry of Plant Cells written by John Lloyd Hall and published by Elsevier-North-Holland Biomedical Press. This book was released on 1978 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research on the Cytochemistry of Cell was of Microorganisms written by Wanda K. Farr and published by . This book was released on 1964 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a part of the research program designed to solve the nutritional problems of astronauts during extended explorations of space, cell wall substances of four fungi, Heterocephalum aurantiacum, Spicaria sp., Cladosporium sp. and Linderina pennispora have been identified and localized by standard cytochemical methods. Chitin is the resistant cell wall material in the component cells of all of these fungi and results of efforts to identify cellulose in the same cell walls have been negative. Excretions which accumulate on the surfaces of the aerial corticating hyphae of H. aurantiacum are composed primarily of calcium salts and an unidentified colloidal material, yellow-orange in color and having many characteristics in common with the so-called "fungal gamboge." Reactions of the previously untreated chitinous cell walls to the enzyme, chitinase, are positive. Enzymatic hydrolysis represents the most suitable method now available for the conversion of chitin to N-acetylglucosamine with the accompanying breakdown of the cell wall and concurrent release of nutritive substances in the protoplast. X-ray diffraction patterns of chitin have been obtained from purified cell wall samples. Measurements of these same samples, at successive stages of removal of non-chitinous materials, show a gradual increase in refractive index values. Progress has been made in the adaptation of interference microscopy to the study of chitinous cell walls during the process of hydrolysis with chitinase.
Download or read book Biochemistry of Cell Walls and Membranes written by C. Fred Fox and published by . This book was released on 1975 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cytophysical and Cytochemical Analysis of Cell Wall Structure in Relation to Enzymatic and Microbial Degradation written by Graeme P. Berlyn and published by . This book was released on 1987 with total page 64 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Cytochemical Study of Transfer Cell Walls written by Liot, Douglas John and published by 1973 [c1974]. This book was released on 1973 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Interpretation of Ultrastructure written by Robert John Cecil Harris and published by . This book was released on 1964 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Cell Biology written by Nicholas Harris and published by Oxford University Press. This book was released on 1994 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell biologists seek to characterize the principles underlying complex phenomena such as growth and differentiation and to define the ways in which plant cells respond to external and internal stimuli. This book discusses established techniques and presents some exciting advances that will have a major impact on the field in the future. The book begins with a detailed discussion of methods and protocols for viewing, staining, and localizing cell components. Subsequent chapters cover topics such as localizing specific nucleic acid sequences and proteins, protoplast research, wall analysis, plant cytoskeleton research, isolation and use of intact chloroplasts and thylakoids, and the measurement of ions and solutes within plant cells. Plant Cell Biology: A Practical Approach provides both newcomers and experienced researchers with a comprehensive practical guide to the subject.
Download or read book Plant Histochemistry and Cytochemistry written by Peter B. Gahan and published by . This book was released on 1984 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopy of Plant Cells written by C Hawes and published by Academic Press. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
