Download or read book Metalloprotein Active Site Assembly written by Michael K. Johnson and published by John Wiley & Sons. This book was released on 2017-08-30 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizes the essential biosynthetic pathways for assembly of metal cofactor sites in functional metalloproteins Metalloprotein Active Site Assembly focuses on the processes that have evolved to orchestrate the assembly of metal cofactor sites in functional metalloproteins. It goes beyond the simple incorporation of single metal ions in a protein framework, and includes metal cluster assembly, metal-cofactor biosynthesis and insertion, and metal-based post-translational modifications of the protein environments that are necessary for function. Several examples of each of these areas have now been identified and studied; the current volume provides the current state-of-the-art understanding of the processes involved. An excellent companion to the earlier book in this series Metals in Cells—which discussed both the positive and negative effects of cellular interactions with metals—this comprehensive book provides a diverse sampling of what is known about metalloprotein active site assembly processes. It covers all major biological transition metal components (Mn, Fe, Co, Ni, Mo), as well as the other inorganic components, metal-binding organic cofactors (e.g., heme, siroheme, cobalamin, molybdopterin), and post-translationally modified metal binding sites that make up the patchwork of evolved biological catalytic sites. The book compares and contrasts the biosynthetic assembly of active sites involving all biological metals. This has never been done before since it is a relatively new, fast-developing area of research. Metalloprotein Active Site Assembly is an ideal text for practitioners of inorganic biochemistry who are studying the biosynthetic pathways and gene clusters involved in active site assembly, and for inorganic chemists who want to apply the concepts learned to potential synthetic pathways to active site mimics.

Download or read book Metalloprotein Active Site Assembly written by Michael K. Johnson and published by John Wiley & Sons. This book was released on 2017-08-14 with total page 941 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizes the essential biosynthetic pathways for assembly of metal cofactor sites in functional metalloproteins Metalloprotein Active Site Assembly focuses on the processes that have evolved to orchestrate the assembly of metal cofactor sites in functional metalloproteins. It goes beyond the simple incorporation of single metal ions in a protein framework, and includes metal cluster assembly, metal-cofactor biosynthesis and insertion, and metal-based post-translational modifications of the protein environments that are necessary for function. Several examples of each of these areas have now been identified and studied; the current volume provides the current state-of-the-art understanding of the processes involved. An excellent companion to the earlier book in this series Metals in Cells—which discussed both the positive and negative effects of cellular interactions with metals—this comprehensive book provides a diverse sampling of what is known about metalloprotein active site assembly processes. It covers all major biological transition metal components (Mn, Fe, Co, Ni, Mo), as well as the other inorganic components, metal-binding organic cofactors (e.g., heme, siroheme, cobalamin, molybdopterin), and post-translationally modified metal binding sites that make up the patchwork of evolved biological catalytic sites. The book compares and contrasts the biosynthetic assembly of active sites involving all biological metals. This has never been done before since it is a relatively new, fast-developing area of research. Metalloprotein Active Site Assembly is an ideal text for practitioners of inorganic biochemistry who are studying the biosynthetic pathways and gene clusters involved in active site assembly, and for inorganic chemists who want to apply the concepts learned to potential synthetic pathways to active site mimics.

Download or read book Handbook on Metalloproteins written by Ivano Bertini and published by CRC Press. This book was released on 2001-06-29 with total page 1382 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Handbook on Metalloproteins focuses on the available structural information of proteins and their metal ion coordination spheres. It centers on the metal ions indispensable for life but also considers metal ions used as substitution probes in studies of metalloproteins. Emphasizing the structure-function relationship, the book covers the common and distinct characterstics of metallo- enzymes, proteins, and amino acids bonded to copper, zinc, iron, and more.

Download or read book Handbook of Metalloproteins Handbook of Metalloproteins written by Albrecht Messerschmidt and published by Wiley-Blackwell. This book was released on 2001 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first two volumes of the "Handbook of Metalloproteins", published in 2001, focused on a number of metals, including iron, nickel, manganese, cobalt, copper and vanadium. Now we are delighted to present volume three which extends the wealth of knowledge and focuses on proteins found in the redox-inactive ions of zinc and calcium. (Midwest).

Download or read book Metalloproteins written by Art E. Cho and published by CRC Press. This book was released on 2015-04-17 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Numerous essential biological functions involve metalloproteins; therefore, understanding metalloproteins and how to manipulate them is significant in the biological and medical fields. An examination of current research, Metalloproteins: Theory, Calculations, and Experiments explores the interplay between theory and experiment, detailing the role

Download or read book Metal Sites in Proteins and Models written by H.A.O. Hill and published by Springer Science & Business Media. This book was released on 1999-04-01 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological chemistry is a major frontier of inorganic chemistry. Three special volumes devoted to Metal Sites in Proteins and Models address the questions: how unusual ("entatic") are metal sites in metalloproteins and metalloenzymes compared to those in small coordination complexes? and if they are special, how do polypeptide chains and co-factors control this? The chapters deal with iron, with metal centres acting as Lewis acids, metals in phosphate enzymes, with vanadium, and with the wide variety of transition metal ions which act as redox centres. They illustrate in particular how the combined armoury of genetics and structure determination at the molecular level are providing unprecedented new tools for molecular engineering.

Download or read book Metal Ions in Biological Systems written by Helmut Sigel and published by CRC Press. This book was released on 1994-02-08 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is devoted solely to the research area of metalloenzymes involving amino acid-residue and related radicals. Topics covered include: general considerations; structure, function and engineering of peroxidases; and ribonucleotide reductase in mammalian systems.

Download or read book Siderophore and Related Natural Products Biosynthesis written by and published by Elsevier. This book was released on 2024-08-20 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology Volume 702 highlights new advances in the field, with this new volume presenting interesting topics on Siderophore and related natural products biosynthesis Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology Updated release includes the latest information on Siderophore and related natural products biosynthesis

Download or read book Mechanisms of Metallocenter Assembly written by Robert P. Hausinger and published by VCH Publishers. This book was released on 1996 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors discuss the importance of metallocenter assembly in the activation of several enzymes and selected structural proteins. The possible reasons why expression of the structural gene is not enough to obtain a functional metalloprotein also are well covered. Mechanisms of Metallocenter Assembly currently is the only book that brings together experimental findings previously scattered in the literature on the mechanisms of metal incorporation into proteins.

Download or read book Bottom up Design of Metalloprotein Assemblies with Diverse Metal Coordination Motifs and Emergent Properties written by Albert Kakkis and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metal ions are indispensable to biological function, as they are utilized for a myriad of processes encompassing signaling, electron transfer, and catalysis. These functions are made possible by exploiting intrinsic properties of the metal ions and the ligands to which they bind, represented by small molecules and metalloproteins. From the perspective of a synthetic chemist, proteins can be conceived as "macromolecular ligands". Like those of small molecule ligands, the properties of "macromolecular ligands" can be parameterized at two discrete coordination spheres. The primary sphere is composed of the amino acid residues and exogenous ligands that coordinate the metal ion, and thus has the most direct impact on metal-based function. The secondary sphere is composed of residues that form mainly non-covalent interactions--hydrogen bonding, hydrophobic, and van der Waals (vDW) interactions--with the primary sphere. Although secondary sphere features do not directly bind the metal ion, they play an indispensable role in controlling metal-based reactivity. For example, the oxygen-binding affinity of myoglobin is influenced significantly by hydrogen bonding interactions between a histidine residue in its secondary sphere and the oxygen ligand that binds the heme co-factor. The large functional scope of metalloproteins is a testament to the wide diversity of primary and secondary spheres, and the efficiency with which metalloproteins execute these functions is a testament to the intricate interplay between the spheres. The functional potential of natural metalloproteins has long inspired protein design efforts. Two major metalloprotein design approaches are rational and de novo design. In rational design, primary and/or secondary sphere features of a natural protein are repurposed to mediate non-native functions. This approach has culminated in the engineering of new-to-nature functions spanning metal-hydride mediated ketone reduction and carbon-silicon bond coupling. While rational design represents an effective path to engineer metal-based functions, such functions are achieved within rigid and highly evolved protein folds/interfaces. Therefore, important questions remain unanswered: how does metal-based function emerge from an initially nonfunctional metalloprotein, and what are the minimum primary/secondary sphere coordination requirements to achieve metal-based functions? The work described in this dissertation stems from a de novo design approach in which both the quaternary structure and metal coordination site(s) of a metalloprotein are designed from scratch. The building block of our designed metalloprotein structures is cytochrome cb562, a natively monomeric, four-helix bundle protein. With cytochrome cb562 as our starting point, we obtain a diverse array of metalloprotein assemblies that serve as platforms for pursuing complex metal-based functions encompassing redox-based signaling and oxygen activation. In Chapter 2, we describe the sequential design of three metalloprotein trimers, TriCyt1, TriCyt2, and TriCyt3. TriCyt1, our initial trimeric construct, is obtained through a single hydrophobic mutation (G70W) at the interface of a cytochrome cb562 variant. Solution experiments indicated that TriCyt1 assembled as a trimer in the presence of all mid-to-late first row transition metals (MnII-ZnII), with yields ranging from 12% (+MnII) to 89% (+CoII). Crystal structures of NiII and CuII-supplemented TriCyt1 revealed a biologically rare His6 coordination motif. The only known natural His6 motif is present in calprotectin, a metal sequestering protein that coordinates MnII with nanomolar affinity. Motivated by the prospect of obtaining from scratch a MnII:His6 site of such high affinity, which had not yet been achieved in protein design, we sought to increase the preorganization of this metal coordination site through redesign of the C2 and C3 interfaces of TriCyt1. Computationally prescribed, mostly hydrophobic mutations at the C3 interface led to TriCyt2, a construct that trimerized with near-quantitative yield in the presence of MnII. A second round of redesign, in which salt bridges were installed at the C2 interface, led to a metal-independent, pH-switchable trimer which bound MnII with ~ 50 nM affinity. While a His6 primary sphere is suitable for achieving high affinity metal binding sites, our ability to design metalloproteins with broad functional potential hinges on our ability to engineer multiple types of metal binding sites. In Chapter 3, we describe our efforts to diversify the primary and/or secondary spheres of the TriCyt scaffold in pursuit of metalloprotein constructs which could coordinate lanthanides with high affinity and selectivity, mediate metal-dependent catalysis, and stabilize multinuclear metal coordination sites. The TriCyt series illustrates metal-ion-identity-independent assembly: the assembly path converges on the same architecture with the same metal coordination motif regardless of the metal ion added. In the TriCyt series there is low cooperativity between metal-ligand and non-covalent interactions, as metal coordination preferences play a minimal role in directing assembly. In Chapter 4, we describe our design and characterization of a variant of Rosetta interface design cytochrome 1 (A74/C96RIDC1) in which the cooperative interplay between metal-ligand and non-covalent interactions could be tuned by the redox state of the protein. In the oxidized state, C96-C96 disulfide bonds rigidify a tetrameric architecture (A74/C96RIDC14ox) whose assembly is independent of metal ion identity. Chemical reduction of the disulfide bonds gives rise to A74/C96RIDC1red, whose assembly is governed in larger part by the metal coordination preferences of exogenous metal ions. Whereas A74/C96RIDC14ox can only access two structural states (apo and metal-bound), A74/C96RIDC1red can access three distinct, metal-ion-identity dependent structural states with unique coordination environments. With five structural states whose accessibility is dependent on solution redox potential and metal ion identity, A74/C96RIDC1 represents a rare protein construct whose oligomerization path is varied through two different types of stimuli. We characterize the oligomeric and conformational states of A74/C96RIDC1 under disulfide-oxidized and disulfide-reduced states and in the presence of different mid-to-late first row transition metal ions, both in solution and via X-ray crystallography. We also employ density functional theory (DFT) and Rosetta interface calculations to attempt to quantify the energetic contributions of metal-ligand and non-covalent interactions and rationalize how differences in metal ion identity result in divergent metal-directed assembly paths.

Download or read book Metal Sites in Proteins and Models written by H.A.O. Hill and published by Springer. This book was released on 1997-08-06 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological chemistry is a major frontier of inorganic chemistry. Three special volumes devoted to Metal Sites in Proteins and Models address the questions: how unusual ("entatic") are metal sites in metalloproteins and metalloenzymes compared to those in small coordination complexes? And if they are special, how do polypeptide chains and co-factors control this? The chapters deal with iron, with metal centres acting as Lewis acids, metals in phosphate enzymes, with vanadium, and with the wide variety of transition metal ions which act as redox centres. They illustrate in particular how the combined armoury of genetics and structure determination at the molecular level are providing unprecedented new tools for molecular engineering.

Download or read book The Heaviest Metals written by William J. Evans and published by John Wiley & Sons. This book was released on 2019-01-08 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: An authoritative survey of the science and advanced technological uses of the actinide and transactinide metals The Heaviest Metals offers an essential resource that covers the fundamentals of the chemical and physical properties of the heaviest metals as well as the most recent advances in their science and technology. The authors – noted experts in the field – offer an authoritative review of the actinide and transactinide elements, i.e., the elements from actinium to lawrencium as well as rutherfordium through organesson, the current end of the periodic table, element 118. The text explores the history of the metals, their occurrence and issues of production, and covers a broad range of chemical subjects including environmental concerns and remediation approaches. The authors also offer information on the most recent and emerging applications of the metals, such as in superconducting materials, catalysis, and research into medical diagnostics. This important resource: Provides an overview of the science and advanced technological uses of the actinide and transactinide metals Describes the basic chemical and physical properties of the heaviest metals, and discusses the challenges and opportunities for their technological applications Contains accessible information on the fundamental features of the heaviest metals, special requirements for their experimental study, and the critical role of computational characterization of their compounds Highlights the most current and emerging applications in areas such as superconducting materials, catalysis, nuclear forensics, and medicine Presents vital contemporary issues of the heaviest metals Written for graduate students and researchers working with the actinide and transactinide elements, industrial and academic inorganic and nuclear chemists, and engineers, The Heaviest Metals is a comprehensive volume that explores the fundamental chemistry and properties of the heaviest metals, and the challenges and opportunities associated with their present and emerging technological uses.

Download or read book Inorganic Battery Materials written by Hailiang Wang and published by John Wiley & Sons. This book was released on 2019-08-23 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Inorganic Battery Materials A guide to the fundamental chemistry and recent advances of battery materials In one comprehensive volume, Inorganic Battery Materials explores the basic chemistry principles, recent advances, and the challenges and opportunities of the current and emerging technologies of battery materials. With contributions from an international panel of experts, this authoritative resource contains information on the fundamental features of battery materials, discussions on material synthesis, structural characterizations and electrochemical reactions. The book explores a wide range of topics including the state-of-the-art lithium ion battery chemistry to more energy-aggressive chemistries involving lithium metal. The authors also include a review of sulfur and oxygen, aqueous battery chemistry, redox flow battery chemistry, solid state battery chemistry and environmentally beneficial carbon dioxide battery chemistry. In the context of renewable energy utilization and transportation electrification, battery technologies have been under more extensive and intensive development than ever. This important book: Provides an understanding of the chemistry of a battery technology Explores battery technology's potential as well as the obstacles that hamper the potential from being realized Highlights new applications and points out the potential growth areas that can serve as inspirations for future research Includes an understanding of the chemistry of battery materials and how they store and convert energy Written for students and academics in the fields of energy materials, electrochemistry, solid state chemistry, inorganic materials chemistry and materials science, Inorganic Battery Materials focuses on the inorganic chemistry of battery materials associated with both current and future battery technologies to provide a unique reference in the field. About EIBC Books The Encyclopedia of Inorganic and Bioinorganic Chemistry (EIBC) was created as an online reference in 2012 by merging the Encyclopedia of Inorganic Chemistry and the Handbook of Metalloproteins. The resulting combination proves to be the defining reference work in the field of inorganic and bioinorganic chemistry, and a lot of chemistry libraries around the world have access to the online version. Many readers, however, prefer to have more concise thematic volumes in print, targeted to their specific area of interest. This feedback from EIBC readers has encouraged the Editors to plan a series of EIBC Books [formerly called EIC Books], focusing on topics of current interest. EIBC Books will appear on a regular basis, will be edited by the EIBC Editors and specialist Guest Editors, and will feature articles from leading scholars in their fields. EIBC Books aim to provide both the starting research student and the confirmed research worker with a critical distillation of the leading concepts in inorganic and bioinorganic chemistry, and provide a structured entry into the fields covered.

Download or read book Sustainable Inorganic Chemistry written by David A. Atwood and published by John Wiley & Sons. This book was released on 2016-10-17 with total page 577 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Earth's natural resources are finite and easily compromised by contamination from industrial chemicals and byproducts from the degradation of consumer products. The growing field of green and sustainable chemistry seeks to address this through the development of products and processes that are environmentally benign while remaining economically viable. Inorganic chemistry plays a critical role in this endeavor in areas such as resource extraction and isolation, renewable energy, catalytic processes, waste minimization and avoidance, and renewable industrial feedstocks. Sustainable Inorganic Chemistry presents a comprehensive overview of the many new developments taking place in this rapidly expanding field, in articles that discuss fundamental concepts alongside cutting-edge developments and applications. The volume includes educational reviews from leading scientists on a broad range of topics including: inorganic resources, sustainable synthetic methods, alternative reaction conditions, heterogeneous catalysis, photocatalysis, sustainable nanomaterials, renewable and clean fuels, water treatment and remediation, waste valorization and life cycle sustainability assessment. The content from this book will be added online to the Encyclopedia of Inorganic and Bioinorganic Chemistry.

Download or read book The Lightest Metals written by Timothy P. Hanusa and published by John Wiley & Sons. This book was released on 2015-11-16 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first seven metals in the periodic table are lithium, beryllium, sodium, magnesium, aluminium, potassium and calcium, known collectively as the “lightest metals”. The growing uses of these seven elements are enmeshing them ever more firmly into critical areas of 21st century technology, including energy storage, catalysis, and various applications of nanoscience. This volume provides comprehensive coverage of the fundamentals and recent advances in the science and technology of the lightest metals. Opening chapters of the book describe major physical and chemical properties of the metals, their occurrence and issues of long-term availability. The book goes on to disucss a broad range of chemical features, including low oxidation state chemistry, organometallics, metal-centered NMR spectroscopy, and cation-π interactions. Current and emerging applications of the metals are presented, including lithium-ion battery technology, hydrogen storage chemistry, superconductor materials, transparent ceramics, nano-enhanced catalysis, and research into photosynthesis and photoelectrochemical cells. The content from this book will be added online to the Encyclopedia of Inorganic and Bioinorganic Chemistry: http://www.wileyonlinelibrary.com/ref/eibc

Download or read book Nickel and Its Surprising Impact in Nature written by Astrid Sigel and published by John Wiley & Sons. This book was released on 2007-03-13 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt: Helmut Sigel, Astrid Sigel and Roland K.O. Sigel, in close cooperation with John Wiley & Sons, launch a new Series “Metal Ions in Life Sciences”. The philosophy of the Series is based on the one successfully applied to a previous series published by another publisher, but the move from “biological systems” to “life sciences” will open the aims and scope and allow for the publication of books touching on the interface between chemistry, biology, pharmacology, biochemistry and medicine. Volume 2 focuses on the vibrant research area concerning nickel as well as its complexes and their role in Nature. With more than 2,800 references and over 130 illustrations, it is an essential resource for scientists working in the wide range from inorganic biochemistry all the way through to medicine. In 17 stimulating chapters, written by 47 internationally recognized experts, Nickel and Its Surprising Impact in Nature highlights critically the biogeochemistry of nickel, its role in the environment, in plants and cyanobacteria, as well as for the gastric pathogen Helicobacter pylori, for gene expression and carcinogenensis. In addition, it covers the complex-forming properties of nickel with amino acids, peptides, phosphates, nucleotides, and nucleic acids. The volume also provides sophisticated insights in the recent progress made in understanding the role of nickel in enzymes such as ureases, hydrogenases, superoxide dismutases, acireductone dioxygenases, acetyl-coenzyme A synthases, carbon monoxide dehydrogenases, methyl-coenzyme M reductases...and it reveals the chaperones of nickel metabolism.

Download or read book Metal Sites in Proteins and Models written by H.A.O. Hill and published by Springer. This book was released on 2014-10-08 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological chemistry is a major frontier of inorganic chemistry. Three special volumes devoted to Metal Sites in Proteins and Models address the questions: how unusual ("entatic") are metal sites in metalloproteins and metalloenzymes compared to those in small coordination complexes? and if they are special, how do polypeptide chains and co-factors control this? The chapters deal with iron, with metal centres acting as Lewis acids, metals in phosphate enzymes, with vanadium, and with the wide variety of transition metal ions which act as redox centres. They illustrate in particular how the combined armoury of genetics and structure determination at the molecular level are providing unprecedented new tools for molecular engineering.