Download or read book Basic Methods in Microscopy written by David L. Spector and published by CSHL Press. This book was released on 2006 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Download or read book Sample Preparation Handbook for Transmission Electron Microscopy written by Jeanne Ayache and published by Springer Science & Business Media. This book was released on 2010-07-03 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.

Download or read book Color Atlas and Manual of Microscopy for Criminalists Chemists and Conservators written by Nicholas Petraco and published by CRC Press. This book was released on 2003-09-29 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Professionals in many disciplines, from archeology to forensic science and anthropology, must be able to identify organic and inorganic fibers and particles. In a single source, this book presents a range of simple methods to help readers quickly characterize and identify a broad range of materials.

Download or read book A Manual of Clinical Diagnosis by Means of Microscopical and Chemical Methods for Students Hospital Physicians and Practitioners written by Charles E. Simon and published by . This book was released on 1902 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X Ray Microanalysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2011-04-14 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.

Download or read book A Manual of Clinical Diagnosis by Means of Microscopical and Chemical Methods written by Charles Edmund Simon and published by . This book was released on 1904 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Manual of Clinical Dianosis by Means of Microscopical and Chemical Methods for Students Hospital Physicians and Practitioners written by Charles Edmund Simon and published by . This book was released on 1902 with total page 670 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Beginners Guide to Scanning Electron Microscopy written by Anwar Ul-Hamid and published by Springer. This book was released on 2018-10-26 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was developed with the goal of providing an easily understood text for those users of the scanning electron microscope (SEM) who have little or no background in the area. The SEM is routinely used to study the surface structure and chemistry of a wide range of biological and synthetic materials at the micrometer to nanometer scale. Ease-of-use, typically facile sample preparation, and straightforward image interpretation, combined with high resolution, high depth of field, and the ability to undertake microchemical and crystallographic analysis, has made scanning electron microscopy one of the most powerful and versatile techniques for characterization today. Indeed, the SEM is a vital tool for the characterization of nanostructured materials and the development of nanotechnology. However, its wide use by professionals with diverse technical backgrounds—including life science, materials science, engineering, forensics, mineralogy, etc., and in various sectors of government, industry, and academia—emphasizes the need for an introductory text providing the basics of effective SEM imaging.A Beginners’ Guide to Scanning Electron Microscopy explains instrumentation, operation, image interpretation and sample preparation in a wide ranging yet succinct and practical text, treating the essential theory of specimen-beam interaction and image formation in a manner that can be effortlessly comprehended by the novice SEM user. This book provides a concise and accessible introduction to the essentials of SEM includes a large number of illustrations specifically chosen to aid readers' understanding of key concepts highlights recent advances in instrumentation, imaging and sample preparation techniques offers examples drawn from a variety of applications that appeal to professionals from diverse backgrounds.

Download or read book Essentials of Polarized Light Microscopy and Ancillary Techniques written by John Gustav Delly and published by . This book was released on 2019-09-09 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual of essentials details the setup and operation of a polarized light microscope for microscopic particle characterization and identification using an approach to applied polarized light microscopy required for applications including industrial problem solving, contaminant identification, trace evidence analysis, and much more.

Download or read book Microscopy Immunohistochemistry and Antigen Retrieval Methods written by M.A. Hayat and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.

Download or read book Live Cell Imaging written by Robert D. Goldman and published by CSHL Press. This book was released on 2005 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

Download or read book Practical Forensic Microscopy written by Barbara P. Wheeler and published by John Wiley & Sons. This book was released on 2011-08-10 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic Microscopy: A Laboratory Manual will provide the student with a practical overview and understanding of the various microscopes and microscopic techniques employed within the field of forensic science. Each laboratory experiment has been carefully designed to cover the variety of evidence disciplines within the forensic science field with carefully set out objectives, explanations of each topic and worksheets to help students compile and analyse their results. The emphasis is placed on the practical aspects of the analysis to enrich student understanding through hands on experience. The experiments move from basic through to specialised and have been developed to cover a variety of evidence disciplines within forensic science field. The emphasis is placed on techniques currently used by trace examiners. This unique, forensic focused, microscopy laboratory manual provides objectives for each topic covered with experiments designed to reinforce what has been learnt along with end of chapter questions, report requirements and numerous references for further reading. Impression evidence such as fingerprints, shoe tread patterns, tool marks and firearms will be analysed using simple stereomicroscopic techniques. Body fluids drug and trace evidence (e.g. paint glass hair fibre) will be covered by a variety of microscopes and specialized microscopic techniques.

Download or read book Biological Electron Microscopy written by Michael J. Dykstra and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

Download or read book Materials Characterization written by Yang Leng and published by John Wiley & Sons. This book was released on 2009-03-04 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers state-of-the-art techniques commonly used in modern materials characterization. Two important aspects of characterization, materials structures and chemical analysis, are included. Widely used techniques, such as metallography (light microscopy), X-ray diffraction, transmission and scanning electron microscopy, are described. In addition, the book introduces advanced techniques, including scanning probe microscopy. The second half of the book accordingly presents techniques such as X-ray energy dispersive spectroscopy (commonly equipped in the scanning electron microscope), fluorescence X-ray spectroscopy, and popular surface analysis techniques (XPS and SIMS). Finally, vibrational spectroscopy (FTIR and Raman) and thermal analysis are also covered.

Download or read book Field Manual of Techniques in Invertebrate Pathology written by Lawrence A. Lacey and published by Springer Science & Business Media. This book was released on 2007-09-27 with total page 852 pages. Available in PDF, EPUB and Kindle. Book excerpt: This field manual is designed to provide background and instruction on a broad spectrum of techniques and their use in the evaluation of entomopathogens in the field. The second edition provides updated information and includes two additional chapters and 12 new contributors. The intended audience includes researchers, graduate students, practitioners of integrated pest management (IPM), regulators and those conducting environmental impact studies of entomopathogens.

Download or read book Electron Microscopy written by John J. Bozzola and published by Jones & Bartlett Learning. This book was released on 1999 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR