Download or read book Manipulating Microscopic Objects A Study on Optical Tweezers and Acoustic Levitation written by Marius Martin and published by GRIN Verlag. This book was released on 2023-03-30 with total page 37 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bachelor Thesis from the year 2023 in the subject Physics - Applied physics, University of Aberdeen, language: English, abstract: This thesis investigates two powerful techniques for manipulating and studying microscopic particles: optical tweezers and acoustic levitation. Optical tweezers use the force of light to trap and manipulate small particles, while acoustic levitation uses sound waves to levitate particles in mid-air. The first part of the thesis focuses on the construction and optimization of an optical tweezers system. The system is build using high-powered laser, optics for focusing the laser beam, and a position detection and control system. the second part of this thesis explores the use of acoustic levitation for the manipulation of micro spheres. The acoustic levitation system is designed and constructed using 72 transducers. The system is characterized using measurements of the acoustic field and the resulting levitation forces. The levitation of micro spheres is demonstrated. This thesis would provide a detailed investigation of two powerful techniques for the manipulation of microscopic particles.
Download or read book Optical Trapping and Manipulation written by Philip Jones and published by MDPI. This book was released on 2020-11-25 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present “Optical Trapping and Manipulation: From Fundamentals to Applications”, a Special Issue of Micromachines dedicated to the latest research in optical trapping. In recognition of the broad impact of optical manipulation techniques across disciplines, this Special Issue collected contributions related to all aspects of optical trapping and manipulation. Both theoretical and experimental studies were welcome, and applications of optical manipulation methods in fields including (but not limited to) single molecule biophysics, cell biology, nanotechnology, atmospheric chemistry, and fundamental optics were particularly welcome in order to showcase the breadth of the current research. The Special Issue accepted diverse forms of contributions, including research papers, short communications, methods, and review articles representing the state-of-the-art in optical trapping.
Download or read book Virtual Environment for Manipulating Microscopic Particles With Optical Tweezers written by and published by DIANE Publishing. This book was released on with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Underdamped Motion of a Microbead in Optical Tweezer and Its Relationship with the Reynolds Number written by Vatsal Asitkumar Joshi and published by . This book was released on 2019 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical tweezers can hold and manipulate microscopic objects with the use of a highly focused laser beam. The radiation pressure acting on the object is generally in the range of piconewtons and can be attractive or repulsive based on the reflective indexes of the object and the surrounding medium. Though the advances in thefield of Optical tweezers have been enormous in the past few years, only the object's planer motion can be analyzed with the motion capture technology available today. Due to the wide applications of Optical tweezers in the field of biology and physics, the ability to simulate the motion of these microscopic objects can help gather more data about object's motion and other related characteristics. One of the major issues related to simulating microscopic objects is the long computation time due to large accelerations. Because the mass of the object is extremely small, even very small amount of force can generate large accelerations resulting in smaller step sizes for numerical integration. This thesis discusses a method to calculate laser beam force acting on the microscopic object. It also discusses equations of motion of the object. The disproportionality caused due to large forces and small mass is mostly addressed by the use of famous overdamped Langevin equations, which omit the inertial properties in the equations of motion. However, this first order model is inconsistent with Newton's second law. A method of multiple scales is introduced which solves this problem by bringing all the terms of equations of motion in proportion with each other thus increasing the time step and reducing computation time. The proposed method was also validated with experiments done on three polystyrene beads with 500nm, 990nm, and 1950nm diameters. A brief discussion is given in the results section about the underdamped behavior of 500nm bead and the significance of low Reynolds number at such small length scale.
Download or read book Fiber Based Optical Trapping and Manipulation written by Baojun Li and published by Nova Science Publishers. This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its first report in 1970 by A. Ashkin, optical trapping and manipulation has been widely used in the interdisciplines of micro- and nano-photonics, biophotonics, biomedicine, etc. A conventional tool for optical trapping and manipulation is the conventional optical tweezer (COT), the core part of which is a free-space focused laser beam. However, manipulation with COTs has some limitations such as manipulation inflexibility, bulky structure, diffraction limitation for nanoparticles, and limited integration functions. The introduction of optical fiber-based optical trapping and manipulation has solved these limitations. Using optical fibers with different configurations, optical trapping and manipulation with multiple functions can be realised with high flexibility, precision, and integration. By launching laser beams with different wavelengths into the fiber, both the photothermal effect and optical force can be used for optical trapping and manipulation. For the optical force manipulation, both evanescent fields at the surface of a subwavelength optical fiber and light output from a fiber end can be used for optical manipulation. The manipulation with light output from a fiber end can be divided into dual fiber tweezers and single fiber tweezers. For single fiber tweezers, one can realise the stable trapping of single particles both in a contact and non-contact manner for further applications. In addition, single fiber tweezers can also be used for multiple particle trapping and cell assembly, which can further be used for the assembly of biophotonic components and devices. Furthermore, by placing microparticles, which act as microlens at the end facet of an optical fiber, the microlens can be served as a photonic nanojet. This fiber supported photonic nanojet can be easily used for the trapping and detection of nanoparticles and biomolecules. Optical fiber-based optical trapping and manipulation have the advantages of easy fabrication, compact configuration, flexible manipulation, easy integration, wide applicability, etc., which provides for a wide application of prospects in micro- and nano-photonics, biophotonics, and biomedicine.
Download or read book Optical Tweezers written by Philip H. Jones and published by Cambridge University Press. This book was released on 2015-12-03 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide to the theory, practice and applications of optical tweezers, combining state-of-the-art research with a strong pedagogic approach.
Download or read book Calibration of an Optical Trap written by Debalina Chakraborty and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A tightly focused laser beam can trap and manipulate microscopic objects by exerting force on them. Optical trapping or laser trapping or laser tweezers is a term widely used in describing the trapping and manipulation of these objects by optical gradient forces. Such devices have many uses in biophysics. This thesis describes the reconstruction of a previously existing optical trapping system that had fallen into disrepair and the work done to calibrate that optical trapping system. The pre-existing system was refurbished and aligned so that optical traps could again be generated. The resulting traps were then calibrated in preparation for further improvements to the trapping strength. Micrometer-sized dielectric spheres were trapped by the tweezers. Trap stiffness was measured using a mean square displacement method from data obtained with two particle tracking programs operating on captured microscope image stacks. The trap stiffnesses thus obtained were checked by applying known hydrodynamic forces to the particles and measuring the displacement. The results show that both the tracking programs (Rytrack and Sphere tracker)generated trap stiffness values closer to the results from hydrodynamic displacement. Trap stiffness was also found to increase with increasing laser power, as expected. The stiffness vs power can be fit by a power law with an exponent of 0.73 for Rytrack and 0.66 for Sphere tracker. The trap stiffness was also weakly dependent on height above the sample plane. Surprisingly, it was found that trap strength differed by some significant factors (sometimes as much as a factor of 2) along perpendicular axes. The source of this asymmetrical trap strength has yet to be determined. In addition, the apparent uncertainty in the trap strength data is much larger than the expected uncertainty from the mean square displacement. Mean square displacement seemed to vary with time. This left a source of systematic error seen in the values of trap stiffness. The source of this additional uncertainty is not clear but may lie in time variations in laser power. This will also be a subject of further work.
Download or read book Microrobotics for Micromanipulation written by Nicolas Chaillet and published by John Wiley & Sons. This book was released on 2013-03-04 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microrobotics is an emerging and booming area with many and various applications, including in fields such as industrial/manufacturing robotics, medical robotics, and laboratory instrumentation. Microrobotics for Micromanipulation presents for the first time, in detail, a treatment of the field of robotics dedicated to handling objects of micrometer dimensions. At these dimensions, the behavior of objects is significantly different from the better known, larger scales, which leads to implementation techniques that can be radically different from the more commonly used solutions. This book details the behaviors of objects at the micrometer scale and provides robotics solutions that are suitable, in terms of actuators, grippers, manipulators, environmental perception, and microtechnology. Worked examples are included in the book - enabling engineers, students and researchers to familiarize themselves with this emerging area and to contribute to its development.
Download or read book Microscale Acoustofluidics written by Thomas Laurell and published by Royal Society of Chemistry. This book was released on 2014-12-08 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: The manipulation of cells and microparticles within microfluidic systems using external forces is valuable for many microscale analytical and bioanalytical applications. Acoustofluidics is the ultrasound-based external forcing of microparticles with microfluidic systems. It has gained much interest because it allows for the simple label-free separation of microparticles based on their mechanical properties without affecting the microparticles themselves. Microscale Acoustofluidics provides an introduction to the field providing the background to the fundamental physics including chapters on governing equations in microfluidics and perturbation theory and ultrasound resonances, acoustic radiation force on small particles, continuum mechanics for ultrasonic particle manipulation, and piezoelectricity and application to the excitation of acoustic fields for ultrasonic particle manipulation. The book also provides information on the design and characterization of ultrasonic particle manipulation devices as well as applications in acoustic trapping and immunoassays. Written by leading experts in the field, the book will appeal to postgraduate students and researchers interested in microfluidics and lab-on-a-chip applications.
Download or read book Optical Trapping and Optical Micromanipulation written by Kishan Dholakia and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Study of Dynamics in Microscopic Systems Using Optical Tweezers written by Thomas James Smart and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of a Dual Joystick controlled Optical Trapping and Cutting System for Optical Micro manipulation of Cells written by Marcellinus Stevie Harsono and published by . This book was released on 2011 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt: In many situations there is a need to physically manipulate microscopic objects with as much dexterity as our own hands provide in the macroscopic world. An example would be applying stresses onto cells in order to determine their mechanical properties. Our existing microscope-laser experimental system is capable of manipulating microscopic objects using two optical tweezers and one optical scissors to move and cut these objects, respectively. Despite these capabilities, however, the point-and-click user interface for optical trapping and cutting was cumbersome and hard to use, limiting the system's potential for micro-manipulation. In order to resolve this limitation, a new, more intuitive and hands-on user interface using two joysticks was designed and developed from the ground up in order to provide responsive, real-time control of the optical tweezers and scissors for microscopic manipulation. This new joystick user interface was then used to verify whether or not forces other than those due to microtubule dynamics act upon chromosomes during mitosis, tested in mitotic PtK2 and Indian Muntjac cells by 1) depolymerizing microtubules using nocodazole, and 2) disrupting microtubules using laser ablation with optical scissors, and subsequently attempting to freely manipulate chromosomes using joystick-controlled optical trapping.
Download or read book A Study on Moving Objects Representation and Reasoning written by Yuniel Proenza and published by GRIN Verlag. This book was released on 2015-12-07 with total page 21 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Essay from the year 2015 in the subject Business economics - Information Management, grade: 7.0, , language: English, abstract: There are some researches that explore and help to solve the representation of moving objects, mainly focused on improving DBMS and Query Languages for support spatio-temporal information storing and processing. Some other researches present techniques to analyze trajectories using data mining, indexing and math operations. More recently, with the emergence of the Semantic Web, the tracking of trajectories of moving objects became a fully applicational field and an open area for ontology applications. This paper explores moving objects representation and reasoning, aiming to find out foundations for a semantic representation of moving objects approach, for solving some existing problems when considering spatio-temporal aspects and semantically enrich query results over any stored information of moving objects.
Download or read book Structured Light Fields written by Mike Wördemann and published by Springer Science & Business Media. This book was released on 2012-05-16 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: The optical trapping of colloidal matter is an unequalled field of technology for enabling precise handling of particles on microscopic scales, solely by the force of light. Although the basic concept of optical tweezers, which are based on a single laser beam, has matured and found a vast number of exciting applications, in particular in the life sciences, there are strong demands for more sophisticated approaches. This thesis gives an introductory overview of existing optical micromanipulation techniques and reviews the state-of-the-art of the emerging field of structured light fields and their applications in optical trapping, micromanipulation, and organisation. The author presents established, and introduces novel concepts for the holographic and non-holographic shaping of a light field. A special emphasis of the work is the demonstration of advanced applications of the thus created structured light fields in optical micromanipulation, utilising various geometries and unconventional light propagation properties. While most of the concepts developed are demonstrated with artificial microscopic reference particles, the work concludes with a comprehensive demonstration of optical control and alignment of bacterial cells, and hierarchical supramolecular organisation utilising dedicated nanocontainer particles.
Download or read book Field Driven Micro and Nanorobots for Biology and Medicine written by Yu Sun and published by Springer Nature. This book was released on 2021-11-25 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the substantial progress recently made in the development of micro and nanorobotic systems, utilizing magnetic, optical, acoustic, electrical, and other actuation fields. It covers several areas of micro and nanorobotics including robotics, materials science, and biomedical engineering. Field-Driven Micro and Nanorobots for Biology and Medicine provides readers with fundamental physics at the micro and nano scales, state-of-the-art technical advances in field-driven micro and nanorobots, and applications in biological and biomedical disciplines.
Download or read book Photorefractive Optoelectronic Tweezers and Their Applications written by Michael Esseling and published by Springer. This book was released on 2014-07-31 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the never-ending quest for miniaturization, optically controlled particle trapping has opened up new possibilities for handling microscopic matter non-invasively. This thesis presents the application of photorefractive crystals as active substrate materials for optoelectronic tweezers. In these tweezers, flexible optical patterns are transformed into electrical forces by a photoconductive material, making it possible to handle matter with very high forces and high throughput. Potential substrate materials’ properties are investigated and ways to tune their figures-of-merit are demonstrated. A large part of the thesis is devoted to potential applications in the field of optofluidics, where photorefractive optoelectronic tweezers are used to trap, sort and guide droplets or particles in microfluidic channels, or to shape liquid polymers into optical elements prior to their solidification. Furthermore, a new surface discharge model is employed to discuss the experimental conditions needed for photorefractive optoelectronic tweezers.
Download or read book 7th International Conference on the Development of Biomedical Engineering in Vietnam BME7 written by Vo Van Toi and published by Springer. This book was released on 2019-06-05 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the proceedings of the 7th International Conference on the Development of Biomedical Engineering in Vietnam which was held from June 27-29, 2018 in Ho Chi Minh City. The volume reflects the progress of Biomedical Engineering and discusses problems and solutions. It aims to identify new challenges, and shaping future directions for research in biomedical engineering fields including medical instrumentation, bioinformatics, biomechanics, medical imaging, drug delivery therapy, regenerative medicine and entrepreneurship in medical devices.
