Download or read book Lupine Anthracnose written by J. L. Weimer and published by . This book was released on 1952 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Diseases of Cultivated Lupines in the Southeast written by J. L. Weimer and published by . This book was released on 1952 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lupine Anthracnose Classic Reprint written by J. J. Weimer and published by Forgotten Books. This book was released on 2017-11-09 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt: Excerpt from Lupine Anthracnose The causal fungus is seed - borne, and can survive for several months in stored seed. Evidence was found that it is largely 011 entirely dead by the second planting time, or after about 18 months' storage. Optimum temperature for mycelial growth of the strain of this fungus used 011 agar is near 25° C. The mycelium made no growth, but was not killed, in 13 days at Very slight growth was made in 5 days at but death occurred in that time at At 51° the spores were killed in 10 minutes. Conidia that. Were removed from pods and kept dry died in less than a month. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
Download or read book Lupine Anthracnose written by J. L. Weimer and published by . This book was released on 1952 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lupin Anthracnose written by and published by . This book was released on 1997 with total page 2 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization and Expression of an Endopolygalacturonase Gene from a Lupin Anthracnose Fungus Identified as Colletotrichum Lupine VAR Setosum written by Hester Catharina Lotter and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Endopolygalacturonases (PGs) are the first cell wall degrading enzymes that are produced when pathogenic fungi encounter the host cell wall (Albersheim and Anderson, 1971). The role that these enzymes play in pathogenicity has been investigated for numerous pathogenic fungi. Although the results are not conclusive, there is evidence for some fungi that these enzymes are significant for their pathogenecity. Furthermore, plants contain polygalacturonase inhibiting proteins (PGIPs) in their cell walls, which are able to inhibit PGs (De Lorenzo et al, 2001: 2002). Colletotrichum SHK2148 is a pathogenic fungus causing anthracnose of lupin plants in South Africa. The identity of the fungus has been described as Colletotrichum tortuosum (Koch, 1996). However, this was based on morphological evidence only. Thus, the classification of the South African lupin- associated Colletotrichum isolates was re-assessed by comparing Colletotrichum SHK2148 on a morphological and molecular level to the recently described Colletotrichum lupinispecies (Nirenberg et al, 2002) as well as previously described Colletotrichum acutatum lupin anthracnose isolates (Talhinas et al, 2002). Based on the culture morphology, ITS and b? -tubulin sequence data, it was concluded that Colletotrichum SHK2148 groups with C. lupini, more specifically, C. lupini var. setosum. The fungus, renamed Colletotrichum lupini SHK2148, was evaluated for its PG activity in pectin media (pH 5) over a 12 day growth period by using an agarose diffusion assay. The specific PG activity reached its highest level after three days, whereafter it decreased. Previous studies performed at the ARC, revealed that the fungus produced PG activity and this crude activity was inhibited by a PGIP produced in apple. A study was launched to isolate and characterise the gene(s) responsible for PG production. PG gene sequences from Colletotrichum gloeosporioides f.sp. malvae and Colletotrichum lindemuthianum were compared and conserved regions were identified from which primers were designed to amplify a fragment of a PG gene from C. lupini SHK2148. Inverse PCR was used to resolve the 5 and 3 sequences of the PG gene whereafter a complete copy of the gene was isolated from the genome of the fungus and characterised. The isolated gene was approximately 1Kb, contained a single intron of 59 bp and was very similar to the PG gene from C. gloeosporioides f.sp. malvae (cmpgII) as well as one of the PG genes (clpg2) from C. lindemuthianum. Southern blot analyses revealed that the gene was present as a single copy in the genome of the fungus. The in vitro expression of the PG gene from C. lupini SHK2148, grown in pectin media (pH 5), was investigated via northern blot analyses as well as RT-PCR, which revealed that the gene was expressed in the same time period that the highest PG activity was observed. A full cDNA copy of the PG gene was isolated using mRNA harvested from mycelia that was grown for 4 days on pectin. The cDNA copy confirmed the predicted intron position of the previously isolated genomic PG gene. Due to the unavailability of a full cDNA copy of the C. lupini SHK2148 PG gene at the time when expression studies were initiated, a complete cDNA copy was constructed by swapping an internal cDNA PG fragment with its counterpart in the complete genomic PG gene copy. The resulting cDNA PG copy was used as a template from which PG constructs were prepared for expression in Pichia pastoris. Constructs containing the PG gene with its native signal peptide, the PG gene with the ;?-MF signal peptide factor as well as hybrid constructs where the N terminal part of the mature PG proteins of Fusarium moniliforme and C. lupini SHK 2148 were exchanged, were transformed into P. pastoris . No PG activity was observed with an agarose diffusion assay for any of the Pichia clones. SDS-PAGE analyses were used to evaluate total protein isolations from the P. pastoris clones. The supernatant and cells of the clones were subjected to western blot analyses using antibodies directed againstAspergillus niger PG as well as F. moniliforme PG. The only positive hybridisation signal was observed between the A. niger antibody and a protein in supernatant extracts of the P. pastoris clones. However, the size of the hybridising band was very large. This could be due to glycosylation of the C. lupini SHK 2148 PG in P. pastoris, although the size increase is unusually large. The results indicated that it is unlikely that the C. lupini SHK 2148 PG was expressed in P. pastoris transformed with any of these constructs. Copyright.
Download or read book Circular written by and published by . This book was released on 1952 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Diseases of Cultivated Lupines in the Southeast written by J. L. Weimer and published by . This book was released on 1952 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Lupin Anthracnose Caused by Colletotrichum Gloeosporioides written by Dawit Solomon Ghebremariam and published by . This book was released on 2001 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lupin Anthracnose Survey Report Spring 2008 written by Greg Bowery and published by . This book was released on 2009 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dogwood Anthracnose and Its Spread in the South written by Robert Lee Anderson and published by . This book was released on 1994 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book 31 year Results in Growing Shelterbelts on the Northern Great Plains written by United States. Department of Agriculture. Production and Marketing Administration. Grain Branch and published by . This book was released on 1953 with total page 1060 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Agriculture Department circular presents solutions on dealing with the proliferation of mesquite on southern Arizona range lands.
Download or read book Subject Heading List Preliminary Edition written by National Agricultural Library (U.S.) and published by . This book was released on 1963 with total page 1238 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Subject Heading List written by National Agricultural Library (U.S.) and published by . This book was released on 1963 with total page 1242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Support for Lupin Anthracnose Control Strategies written by G. Thomas and published by . This book was released on 1999 with total page 26 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Yearbook of Agriculture written by and published by . This book was released on 1953 with total page 994 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Introduction to Plant Diseases written by George B. Lucas and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every year we see a remarkable increase in scientific knowledge. We are learning more each day about the world around us, about the numerous biological organisms of the biosphere, about the physical and chemical processes that shaped and continue to change our planet. The cataloging, retrieval, dissemination, and use of this new information along with the continued development of new computer technology provide some of the most challenging problems in science as we enter the Information Age. With the explosion of knowledge in science, it is especially important that students in introductory courses learn not only the basic material of a subject, but also about the newest developments in that subject. With this goal in mind, we have prepared a second edition of Introduction to Plant Diseases: Identification and Management. We prepared this edition with the same general purpose that we had for the first edition - to provide practical, up-to-date information that helps in the successful management of diseases on food, fiber, and landscape plants for students who do not have a strong background in the biological sciences. We included new information on (1) the precise identification of diseases and the pathogens that cause them, (2) the development of epidemics of plant diseases, (3) the application of biotechnology in plant pathology, (4) the use of alternative methods of crop production and disease management that help protect the environment, and (5) diseases that have become more important since the first edition was published.
