Download or read book Leveraging Open Microfluidics for Platform Development and Cell signaling Studies In Vitro written by Samuel Berry and published by . This book was released on 2020 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation discusses the fundamentals, development, validation, and application of open microfluidic technologies as a research tool in biological studies. Open microfluidics is a rapidly evolving and expanding field, characterized by the study of fluid behavior in channels with dimensions

Download or read book Development of a Novel Microfluidic Platform to Study T Cell Signaling written by Shannon L. Faley and published by . This book was released on 2007 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studying Intercellular Signaling Underlying Human Diseases Using Open Microfluidic Coculture written by Tianzi Zhang and published by . This book was released on 2020 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation focuses on the development of innovative open microfluidic cell culture platforms and their application in studying intercellular signaling underlying human diseases in controlled ex vivo microenvironments. Chapter 1 introduces the background of open microfluidic capillary systems, including the design considerations and current fabrication techniques, and addresses the advantages of using open microfluidic cell culture systems to study intercellular signaling. Chapter 2 presents a new open microfluidic capillary platform, which patterns biocompatible hydrogel walls along a rail insert set inside established cultureware. The permeable hydrogel walls provide segregation for the cells and support diffusion of soluble factors. Chapter 3 discusses a microscale collagen gel contraction assay with an engineered well plate insert that uses surface tension forces to load and manipulate small volumes of cell-laden collagen. The system is easily operated with two pipetting steps and the microscale device moves dynamically as a result of cellular forces. Chapter 4 presents an open microfluidic coculture platform consisting of two independent cell culture regions separated by a half wall. The cell types are selectively seeded into the regions and connected with cell culture media. Using the device, we show that human kidney tubular epithelial cells can tune organ-specificity in other endothelial cell types. Chapter 5 shows a preliminary asthma disease coculture model of human lung fibroblasts and primary alveolar macrophages using a similar open microfluidic coculture device to that described in Chapter 4. The preliminary cell culture result indicates that lung fibroblasts exhibit elevated cell contractility when cultured separately with alveolar macrophages in shared cell culture media. Chapter 6 concludes the thesis and proposes some future research directions and interesting metabolomic studies using existing or alternative open microfluidic systems.

Download or read book Lab on a Chip Fabrication and Application written by Margarita Stoytcheva and published by BoD – Books on Demand. This book was released on 2016-06-29 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: The necessity of on-site, fast, sensitive, and cheap complex laboratory analysis, associated with the advances in the microfabrication technologies and the microfluidics, made it possible for the creation of the innovative device lab-on-a-chip (LOC), by which we would be able to scale a single or multiple laboratory processes down to a chip format. The present book is dedicated to the LOC devices from two points of view: LOC fabrication and LOC application.

Download or read book Open Microfluidics written by Jean Berthier and published by John Wiley & Sons. This book was released on 2016-07-20 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Open microfluidics or open-surface is becoming fundamental in scientific domains such as biotechnology, biology and space. First, such systems and devices based on open microfluidics make use of capillary forces to move fluids, without any need for external energy. Second, the "openness" of the flow facilitates the accessibility to the liquid in biotechnology and biology, and reduces the weight in space applications. This book has been conceived to give the reader the fundamental basis of open microfluidics. It covers successively The theory of spontaneous capillary flow, with the general conditions for spontaneous capillary flow, and the dynamic aspects of such flows. The formation of capillary filaments which are associated to small contact angles and sharp grooves. The study of capillary flow in open rectangular, pseudo-rectangular and trapezoidal open microchannels. The dynamics of open capillary flows in grooves with a focus on capillary resistors. The case of very viscous liquids is analyzed. An analysis of suspended capillary flows: such flows move in suspended channels devoid of top cover and bottom plate. Their accessibility is reinforced, and such systems are becoming fundamental in biology. An analysis of “rails” microfluidics, which are flows that move in channels devoid of side walls. This geometry has the advantage to be compatible with capillary networks, which are now of great interest in biotechnology, for molecular detection for example. Paper-based microfluidics where liquids wick flat paper matrix. Applications concern bioassays such as point of care devices (POC). Thread-based microfluidics is a new domain of investigation. It is seeing presently many new developments in the domain of separation and filtration, and opens the way to smart bandages and tissue engineering. The book is intended to cover the theoretical aspects of open microfluidics, experimental approaches, and examples of application.

Download or read book Introduction to BioMEMS written by Albert Folch and published by CRC Press. This book was released on 2016-04-19 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: The entire scope of the BioMEMS field-at your fingertipsHelping to educate the new generation of engineers and biologists, Introduction to BioMEMS explains how certain problems in biology and medicine benefit from and often require the miniaturization of devices. The book covers the whole breadth of this dynamic field, including classical microfabr

Download or read book Microscale Acoustofluidics written by Thomas Laurell and published by Royal Society of Chemistry. This book was released on 2014-12-08 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: The manipulation of cells and microparticles within microfluidic systems using external forces is valuable for many microscale analytical and bioanalytical applications. Acoustofluidics is the ultrasound-based external forcing of microparticles with microfluidic systems. It has gained much interest because it allows for the simple label-free separation of microparticles based on their mechanical properties without affecting the microparticles themselves. Microscale Acoustofluidics provides an introduction to the field providing the background to the fundamental physics including chapters on governing equations in microfluidics and perturbation theory and ultrasound resonances, acoustic radiation force on small particles, continuum mechanics for ultrasonic particle manipulation, and piezoelectricity and application to the excitation of acoustic fields for ultrasonic particle manipulation. The book also provides information on the design and characterization of ultrasonic particle manipulation devices as well as applications in acoustic trapping and immunoassays. Written by leading experts in the field, the book will appeal to postgraduate students and researchers interested in microfluidics and lab-on-a-chip applications.

Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.

Download or read book Introductory Bioelectronics written by Ronald R. Pethig and published by John Wiley & Sons. This book was released on 2012-08-22 with total page 413 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioelectronics is a rich field of research involving the application of electronics engineering principles to biology, medicine, and the health sciences. With its interdisciplinary nature, bioelectronics spans state-of-the-art research at the interface between the life sciences, engineering and physical sciences. Introductory Bioelectronics offers a concise overview of the field and teaches the fundamentals of biochemical, biophysical, electrical, and physiological concepts relevant to bioelectronics. It is the first book to bring together these various topics, and to explain the basic theory and practical applications at an introductory level. The authors describe and contextualise the science by examining recent research and commercial applications. They also cover the design methods and forms of instrumentation that are required in the application of bioelectronics technology. The result is a unique book with the following key features: an interdisciplinary approach, which develops theory through practical examples and clinical applications, and delivers the necessary biological knowledge from an electronic engineer’s perspective a problem section in each chapter that readers can use for self-assessment, with model answers given at the end of the book along with references to key scientific publications discussions of new developments in the bioelectronics and biosensors fields, such as microfluidic devices and nanotechnology Supplying the tools to succeed, this text is the best resource for engineering and physical sciences students in bioelectronics, biomedical engineering and micro/nano-engineering. Not only that, it is also a resource for researchers without formal training in biology, who are entering PhD programmes or working on industrial projects in these areas.

Download or read book Open Channel Microfluidics written by Jean Berthier and published by Morgan & Claypool Publishers. This book was released on 2019-09-04 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Open microfluidics, the study of microflows having a boundary with surrounding air, encompasses different aspects such as paper or thread-based microfluidics, droplet microfluidics and open-channel microfluidics. Open-channel microflow is a flow at the micro-scale, guided by solid structures, and having at least a free boundary (with air or vapor) other than the advancing meniscus. This book is devoted to the study of open-channel microfluidics which (contrary to paper or thread or droplet microfluidics) is still very sparsely documented, but bears many new applications in biology, biotechnology, medicine, material and space sciences. Capillarity being the principal force triggering an open microflow, the principles of capillarity are first recalled. The onset of open-channel microflow is next analyzed and the fundamental notion of generalized Cassie angle (the apparent contact angle which accounts for the presence of air) is presented. The theory of the dynamics of open-channel microflows is then developed, using the notion of averaged friction length which accounts for the presence of air along the boundaries of the flow domain. Different channel morphologies are studied and geometrical features such as valves and capillary pumps are examined. An introduction to two-phase open-channel microflows is also presented showing that immiscible plugs can be transported by an open-channel flow. Finally, a selection of interesting applications in the domains of space, materials, medicine and biology is presented, showing the potentialities of open-channel microfluidics.

Download or read book Microfluidic Cell Culture Systems written by Christopher Bettinger and published by William Andrew. This book was released on 2012-12-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products.

Download or read book Advanced Healthcare Materials written by Ashutosh Tiwari and published by John Wiley & Sons. This book was released on 2014-05-09 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a comprehensive and interdisciplinary view of cutting-edge research on advanced materials for healthcare technology and applications Advanced healthcare materials are attracting strong interest in fundamental as well as applied medical science and technology. This book summarizes the current state of knowledge in the field of advanced materials for functional therapeutics, point-of-care diagnostics, translational materials, and up-and-coming bioengineering devices. Advanced Healthcare Materials highlights the key features that enable the design of stimuli-responsive smart nanoparticles, novel biomaterials, and nano/micro devices for either diagnosis or therapy, or both, called theranostics. It also presents the latest advancements in healthcare materials and medical technology. The senior researchers from global knowledge centers have written topics including: State-of-the-art of biomaterials for human health Micro- and nanoparticles and their application in biosensors The role of immunoassays Stimuli-responsive smart nanoparticles Diagnosis and treatment of cancer Advanced materials for biomedical application and drug delivery Nanoparticles for diagnosis and/or treatment of Alzheimers disease Hierarchical modelling of elastic behavior of human dental tissue Biodegradable porous hydrogels Hydrogels in tissue engineering, drug delivery, and wound care Modified natural zeolites Supramolecular hydrogels based on cyclodextrin poly(pseudo)rotaxane Polyhydroxyalkanoate-based biomaterials Biomimetic molecularly imprinted polymers

Download or read book Neural Network Models of Cognition written by J.W. Donahoe and published by Elsevier. This book was released on 1997-09-26 with total page 601 pages. Available in PDF, EPUB and Kindle. Book excerpt: This internationally authored volume presents major findings, concepts, and methods of behavioral neuroscience coordinated with their simulation via neural networks. A central theme is that biobehaviorally constrained simulations provide a rigorous means to explore the implications of relatively simple processes for the understanding of cognition (complex behavior). Neural networks are held to serve the same function for behavioral neuroscience as population genetics for evolutionary science. The volume is divided into six sections, each of which includes both experimental and simulation research: (1) neurodevelopment and genetic algorithms, (2) synaptic plasticity (LTP), (3) sensory/hippocampal systems, (4) motor systems, (5) plasticity in large neural systems (reinforcement learning), and (6) neural imaging and language. The volume also includes an integrated reference section and a comprehensive index.

Download or read book Multi Omics for the Understanding of Brain Diseases written by and published by . This book was released on 2021-12-13 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advanced Elastomers written by Anna Boczkowska and published by BoD – Books on Demand. This book was released on 2012-09-12 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an extensive overview of current trends in the area of elastomers and their composites from the chapters contributed by internationally recognized specialists. The book deals with novel synthesis, modelling and experimental methods in elastomers. Contents include: new approach to crosslinking, liquid crystal elastomers, nanocomposites, smart elastomers, elastomers in microelectronics and microfluidics, elastomers in cement concrete and mortar, experimental testing and modelling. Each section demonstrates how enhancements in materials, processes and characterization techniques can improve performance in the field of engineering. The book provides a unique opportunity to discover the latest research on elastomer advances from laboratories around the world. This book addresses to industrial and academic researchers in the fields of physical, chemical, biological sciences and engineering.

Download or read book Microfluidics Based Microsystems written by S. Kakaç and published by Springer. This book was released on 2010-06-30 with total page 621 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains an archival record of the NATO Advanced Study Institute on Microfluidics Based Microsystems – Fundamentals and App- cations held in Çe ?me-Izmir, Turkey, August 23–September 4, 2009. ASIs are intended to be high-level teaching activity in scientific and technical areas of current concern. In this volume, the reader may find interesting chapters and various microsystems fundamentals and applications. As the world becomes increasingly concerned with terrorism, early - spot detection of terrorist’s weapons, particularly bio-weapons agents such as bacteria and viruses are extremely important. NATO Public Diplomacy division, Science for Peace and Security section support research, Advanced Study Institutes and workshops related to security. Keeping this policy of NATO in mind, we made such a proposal on Microsystems for security. We are very happy that leading experts agreed to come and lecture in this important NATO ASI. We will see many examples that will show us Microfluidics usefulness for rapid diagnostics following a bioterrorism attack. For the applications in national security and anti-terrorism, microfluidic system technology must meet the challenges. To develop microsystems for security and to provide a comprehensive state-of-the-art assessment of the existing research and applications by treating the subject in considerable depth through lectures from eminent professionals in the field, through discussions and panel sessions are very beneficial for young scientists in the field.

Download or read book 3D Printed Microfluidic Devices written by Savas Tasoglu and published by MDPI. This book was released on 2019-01-10 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "3D Printed Microfluidic Devices" that was published in Micromachines