Download or read book Lectin Purification and Analysis written by Jun Hirabayashi and published by . This book was released on 2020 with total page 699 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lectins Analytical Technologies written by Carol L. Nilsson and published by Elsevier. This book was released on 2011-10-13 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lectins: Analytical Technologies covers both analytical and biological aspects of lectins (functional carbohydrate (complex sugar) recognition proteins) and provides researchers in the field with a resource containing background information and 'look-up' tables detailing lectin specificity and structures. Also included are methods and practical tips for designing new lectins from existing non-lectin proteins, automated approaches to lectin proteomics and high resolution mass spectrometry techniques. This book will be of interest to both novice and advanced researchers in biomedical, analytical and pharmaceutical fields who are involved in the study of lectin structures or who utilize lectins as analytical tools. The study of lectins and their employment in analytical settings spans a range of fields including: * Crystallography and lectin structure databases* Carbohydrate microarrays for lectin characterization and glycotope identification* Proteomic approaches to the functional identification of bacterial adhesins* Generation of lectins from enzymes* Probing cell-surface lectins with neoglycoconjugates* Reviews up-to-date techniques, including practical hints for laboratory work* Provides overview of lectin e-resources and several color illustrations* Includes a 'look-up' table detailing lectin specificity
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Download or read book Lectin Purification and Analysis written by Jun Hirabayashi and published by Humana. This book was released on 2020-04-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.
Download or read book Plant Lectins written by A. Pusztai and published by Cambridge University Press. This book was released on 1991 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume surveys the chemistry, biochemistry, biosynthesis, metabolism and pharmacological properties of lectins. Lectins, which are most commonly found in plants, are widespread natural products with striking biological activities. Their specific ability to recognise and bind to simple or complex saccharides facilitates their role as effective information protein molecules. As agents of cell-to-cell recognition, lectins promote symbiosis between plants and specific nitrogen-fixing soil bacteria. As natural defensive molecules, they can protect plants against predators such as bacteria, fungi and insects. As part of our diet, lectins are powerful exogenous growth factors in the small intestine and influence our health, the digestive function and the bacterial ecology of the alimentary tract. Lectins are also important research tools in preparative biochemistry and cell science.
Download or read book Lectins written by Jun Hirabayashi and published by Humana Press. This book was released on 2014-08-13 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.
Download or read book Lectins and Glycobiology written by Hans-Joachim Gabius and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: The intriguing complexity, precision, and regulation of the wide range of biologi cal processes is determined by intricate mechanisms of molecular recognition. Their nature is under intense scrutiny. In addition to the well-appreciated interac tion of proteins either with amino acid or nucleotide sequences, the investigation of their interplay with carbohydrate elements of cellular glycoconjugates current ly exerts increasing attraction. In the group of carbohydrate-binding proteins, lec tins are distinguished from antibodies or ligand-affecting enzymes, according to the most recent definition. The thorough analysis of their structure and function is considered as a focus to collect a critical mass of information for delineating details of a further array of biochemical processes with pivotal physiological im pact. Following an already century-long history of scientific description, reflected by subjectively chosen highlights (see the Brief History of Lectin Research at page VI), the excitement in glycobiological research that prevails today can easily be ex plained by our growing awareness of the multifarious significance of a sugar-code system of biological information. This present notion unmistakably has an im pact on lines of research in diverse disciplines like cell and molecular biology, histochemistry, or clinical sciences. It also prompts inherent practical questions such as how to obtain lectins, or how to employ them as instruments in various assay systems with the best possible results. Thus, this book is devoted intentionally to cover the techniques in different research fields that deal with lectins.
Download or read book Protein Analysis and Purification written by I.M. Rosenberg and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip ment other than encouragement to read the manual, if you can find it. Through out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.
Download or read book Hemagglutinins written by Tzi Bun Ng and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hemagglutinins refers to glycoproteins which bring about agglutination of erythrocytes or hemagglutination. Hemagglutination can be used to identify surface antigens on erythrocytes (with known antibodies) and, hence, the blood type of an individual. Hemagglutinins consist of lectins and antibodies. Lectins (from the Latin legere, "to select") are non-immune glycoproteins that exhibit reversible binding to specific carbohydrate structures, glycans of glycoproteins, glycolipids and polysaccharides. Lectins own at least one non-catalytic domain, and, in some cases, a few carbohydrate binding domains, which enable them to effect agglutination of erythrocytes and other cells or precipitation of glycoconjugates. Lectins are found in a constellation of organisms and display an array of activities. Topics discussed in this book encompass algal lectins, plant type 2 ribosome inactivating proteins, edible legume lectins, jacalin, jacalin-related lectin, wheat lectins, rice lectins, banana lectins and potato lectin, immunomodulatory action of plant lectins, piezoelectric assay using the galactose-binding Bauhinia monandra leaf lectin and its antibody as a potent tool for detection of antigen-antibody recognition, assays using hemagglutinins of different origins to investigate their mechanisms of action, lectins from medicinal herbs and medicinal mushrooms, C-type lectins from a diversity of invertebrates and vertebrates, fish lectins and amphibian lectins.
Download or read book A General Method for Lectin Purification by Affinity Chromatography on Polyacrylamide Gels Containing Reductively Aminated Carbohydrate written by Reinhold Johannes Baues and published by . This book was released on 1976 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lectins written by Jun Hirabayashi and published by Humana. This book was released on 2016-09-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.
Download or read book Lectins written by Nathan Sharon and published by Springer Science & Business Media. This book was released on 2007-10 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Second Edition brings readers up to date with all the latest findings in lectin research. Throughout this new edition, more than 200 figures and some thirty tables help readers visualize and understand key concepts and processes. The book starts with an overview of lectin research followed by a survey of the occurrence of lectins in nature. Other areas covered include the nutritional effects of lectins and their functions in nature.
Download or read book Molecular Interactions in Bioseparations written by That T. Ngo and published by Springer. This book was released on 2013-06-20 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Download or read book Lectin Arrays for Glycoprotein Analysis and for the Characterization and Fractionation of Mammalian Cells written by Ting Zheng and published by . This book was released on 2006 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Annona Muricata Lectin Purification and Characterization with Mosquito Activity written by Parthiban E and published by Independent Author. This book was released on 2023-04-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book "Purification and Characterization of Annona Muricata Lectin with Anti-Mosquito Activity" by Parthiban E is a comprehensive guide on the study of a lectin extracted from the seed kernel of the Annona Muricata plant. The book covers the entire process of purification and molecular characterization of the lectin, and its efficacy as an anti-mosquito agent. The book begins with an introduction to Annona Muricata and its various parts, including the seed kernel. The author then explains the process of lectin extraction from the seed kernel and its purification using various chromatographic techniques. The molecular characterization of the purified lectin is also discussed in detail, including its structural and functional properties. One of the most significant aspects of the book is its focus on the anti-mosquito activity of the Annona Muricata lectin. The author provides extensive research and data on the lectin's ability to disrupt the development and growth of mosquito larvae, as well as its impact on adult mosquito behavior. Overall, "Purification and Characterization of Annona Muricata Lectin with Anti-Mosquito Activity" is an essential guide for researchers and professionals in the fields of biotechnology, biochemistry, and entomology. The book's detailed insights and scientific data on the lectin's properties and potential applications make it a valuable resource for anyone interested in the study of lectins and their biological activity.
Download or read book Lectins and Glycobiology written by Hans-Joachim Gabius and published by . This book was released on 1993 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: The intriguing complexity, precision, and regulation of the wide range of biologi cal processes is determined by intricate mechanisms of molecular recognition. Their nature is under intense scrutiny. In addition to the well-appreciated interac tion of proteins either with amino acid or nucleotide sequences, the investigation of their interplay with carbohydrate elements of cellular glycoconjugates current ly exerts increasing attraction. In the group of carbohydrate-binding proteins, lec tins are distinguished from antibodies or ligand-affecting enzymes, according to the most recent definition. The thorough analysis of their structure and function is considered as a focus to collect a critical mass of information for delineating details of a further array of biochemical processes with pivotal physiological im pact. Following an already century-long history of scientific description, reflected by subjectively chosen highlights (see the Brief History of Lectin Research at page VI), the excitement in glycobiological research that prevails today can easily be ex plained by our growing awareness of the multifarious significance of a sugar-code system of biological information. This present notion unmistakably has an im pact on lines of research in diverse disciplines like cell and molecular biology, histochemistry, or clinical sciences. It also prompts inherent practical questions such as how to obtain lectins, or how to employ them as instruments in various assay systems with the best possible results. Thus, this book is devoted intentionally to cover the techniques in different research fields that deal with lectins.
Download or read book Purification Characterization and Localization of an Endogenous Lectin from Quail Intestine microform written by Rixun Fang and published by National Library of Canada. This book was released on 1989 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
