Download or read book Laser induced Fluorescence Studies of Complex Molecules written by Kah Hieng Wong and published by . This book was released on 1988 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Laser Induced Fluorescence Studies in the Gas Phase written by Christopher Guy Atkins and published by . This book was released on 1986 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Chemical and Biochemical Applications of Lasers V4 written by C. Bradley Moore and published by Elsevier. This book was released on 2012-12-02 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical and Biochemical Applications of Lasers, Volume IV focuses on the practical applications of standard commercial laser systems. This book examines the structural studies of DNA by fluorescence microscopy and discusses photochemistry and structural spectroscopy. Organized into eight chapters, this volume starts with an overview of a few cases of laser-induced fluorescence studies of biological molecules. This text then examines the sharp fluorescence spectra of complex molecules in solids that are obtained when a narrow-band laser selectively excites molecules in particular sites. Other chapters describe the theory and application of resonance Raman spectroscopy to various biological systems. This book provides as well a thorough treatment of coherent anti-Stokes Raman spectroscopy and its application in combustion diagnostics and analytical chemistry. The final chapter explores laser control of the sequential photochemical reaction of the drug psoralen with the two strands of the DNA double helix. Physicists, chemists, electrochemists, and chemical engineers will find this book useful.

Download or read book Low Temperature Laser induced Fluorescence Studies of Chromophores in Soft Solids and Biological Matter written by Chen Lin and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Low-temperature laser-induced fluorescence spectroscopy has various applications in analytical, physical, and biophysical chemistry. This technique provides information on the fluorescence origin band, zero-phonon lines and phonon-sidebands, inhomogeneous broadening, electron-phonon coupling strength, and ground- and excited-state vibrational frequencies of studied molecules. Examples discussed in this work include studies of DNA/metabolites and monoclonal antibody (mAb)/antigen interactions. The structural basis for the increased reactivity of BPDE towards guanines at 5-methylcytosine ([superscript]M[superscripte]eC):G sites in DNA was investigated by low temperature laser-based spectroscopy, studying the nature of physical complexes of benzo[a]pyrene tetraol in a series of 5-methylcytosine structural DNA analogs. We found that the presence of a C-5 substituent on cytosine and related structural modifications influences the conformation of BPT in DNA analogs, and could explain the increase in guanine reactivity at [superscript]M[superscript]eC:G sites of the p53 tumor suppressor gene that contains endogenenous 5-([superscript]M[superscript]eC. It has been demonstrated that various mAbs can bind a particular cross-reactant by adopting two distinct "red" and "blue" conformations of its binding sites. We showed that the blue conformation of pyrene in several mAbs (including 4D5 mAb) is consistent with [pi]-cation interactions, underscoring the importance of [pi]-cation interaction in ligand binding. We propose that considerable narrowing of the fluorescence origin band of the ligand in the protein environment could be regarded as a simple indicator of [pi]-cation interactions. It is also shown that time-resolved delta fluorescence line-narrowing ([delta]FLN) spectroscopy, using excitation within the (0,0)-transition band, provides more reliable information of the frequency dependence of the electron-phonon coupling (Huang-Rhys factor, (S

Download or read book Laser Induced Fluorescence Studies of the Ground and Lowest Singlet Excited State of S cis and S trans 2 2 dithienyl written by Janice E. Chadwick and published by . This book was released on 1991 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Chemical and Biochemical Applications of Lasers written by Charles Bradley Moore and published by . This book was released on 1974 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analysis by Capillary Electrophoresis with Laser induced Fluorescence Detection written by Tara Lee Massie and published by . This book was released on 2010 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a versatile and sensitive analytical tool with many potential applications. Its advantages over other chromatographic methods include minimal solvent and sample requirements, low waste volumes, simple instrumentation, and diversity of analytes studied. CE is one of the most efficient separation techniques available for the analysis of both large and small molecules, but because few analytes are natively fluorescent, it is necessary to bind them with fluorescent tags if LIF detection is to be employed. These tags increase sensitivity and provide additional separation from components of the sample that do not bind the tags. In this dissertation, four applications of CE-LIF for the determination of tagged analytes are documented. First, a rapid CE-LIF method was developed for the determination of gamma-aminobutyric acid (GABA) and other important brain biogenic amines and amino acids present in biological samples at low levels. The high efficiency and sensitivity necessary to identify toxicologically relevant changes in neurotransmitter levels in brain tissue and microdialysates following Mn exposure was achieved, and the method required minimum sample work-up and quantities of sample for analysis. A linear relationship between the concentration of GABA and the CE-LIF peak height was established, with correlation coefficients of 0.989 or better for four neurotransmitters. Second, the use of two squarylium-based fluorescent dyes (bis-SQHN-4d and SQHN-3c) as noncovalent protein labels was investigated. The fluorescence emissions of both dyes were enhanced upon complexation with model proteins, demonstrating promise as noncovalent fluorescent protein labels. Third, the nature of interactions between two boronic acid-based probes (SQTM-BA1 and ANQW-BA1) and two model proteins (bovine serum albumin (BSA) and hemagglutinin (HA)) have been explored. The complex between SQTM-BA1 and BSA was the only complex to show increased fluorescence emission, and the role of electrostatic interactions between SQTM-BA1 and BSA in complex formation was explored. Lastly, CE-LIF methods were developed to label and quantify turnip yellow mosaic virus (TYMV). Noncovalent labels Red-1c and NN127 effectively labeled the surface proteins of the virus, and the labeled viruses were then analyzed by CE-LIF. The CE-LIF methods developed herein for the analysis of neurotransmitters, free solution proteins, and viruses demonstrate the versatility of the technique, especially when coupled with fluorescent labeling.

Download or read book Applications of Laser Induced Fluorescence Detection in Biomedical Analysis written by John Emerson Kuo and published by . This book was released on 1985 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Laser induced fluorescence Studies of Fragment Ions written by and published by . This book was released on 1981 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The dynamics of ion-molecule interactions within a mass selective rf quadrupole ion trap are studied for several ion-molecule systems. Laser induced fluorescence is used as a probe of the internal energy distributions of molecular ions under collision free conditions and under controlled collision conditions. The effects of collisions at near thermal energies (0.3 to 0.5 eV) are easily understood in terms of processes such as charge transfer and other energy transfer mechanisms. The A1PI - X1.sigma. system of CH and CD has been examined under collision free conditions. The ions were produced from methane through electron impact ionization/dissociation. The observed energy distributions reflect the dynamical partitioning of dissociation exothermicity, excepting short lived electronic states. Many new transitions belonging to this electronic system have been observed and a reliable vibrational frequency for the X1.sigma./sup +/ state has been obtained. The radiative lifetimes of CH/sup +/ and CD/sup +/ A1PI(v = 0) states have been measured and a revised oscillator strength for the A-X transition has been derived from this data.

Download or read book Multiphoton Laser induced fluorescence Studies of Simple Species written by and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent studies have demonstrated multiple-photon excitation of atomic species. Bischel and coworkers have provided a detailed description of two-photon excitation fluorescence in the detection of atoms generated in a low pressure discharge and its possible application as a diagnostic tool in flame and plasmas. It is also believed that such techniques can be useful in detecting molecular transients which are difficult to detect otherwise as demonstrated in two-photon laser-induced fluorescence (LIF) detection of NO. In this paper, we discuss our recent two-photon LIF studies on I and Br atoms, which are produced via laser photolysis of molecular precursors. The two-photon LIF study of HS and DS radicals is presented as a test case for the detection of other important radical species such as C2H and CH3, which are currently being investigated in our laboratory. In addition, excitation of three-photon resonances of I2, N2, and H2 is discussed.

Download or read book Laser induced Fluorescence for Medical Diagnostics written by Stefan Andersson-Engels and published by . This book was released on 1989 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Laser induced Fluorescence Spectroscopy of Rb Atoms and Hg 2 Molecules microform written by Jan Szczęsny Supronowicz and published by National Library of Canada. This book was released on 1985 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Laser Induced Fluorescence Studies of Molecular Beams written by Steven John Bullman and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Laser induced Fluorescence Studies of Molecular Dynamics written by Michael Joseph Gurnick and published by . This book was released on 1982 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Laser induced Fluorescence Studies of Imino Compounds written by Hassime Traore and published by . This book was released on 1995 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies of Molecular Collisions by Laser Induced Fluorescence written by José Francisco Córdova and published by . This book was released on 1980 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Demonstration of the Feasibility of Laser Induced Fluorescence for Arc Jet Flow Diagnostics written by National Aeronautics and Space Administration (NASA) and published by Createspace Independent Publishing Platform. This book was released on 2018-07 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser Induced Fluorescence (LIF) studies are carried out on nitric oxide and oxygen molecules in the arc jet flows at the NASA Johnson Space Center Reentry Testing Facility. Measurements are taken in the free stream and from a blunt body shock layer. Tests are performed under different flow conditions to determine the feasibility and sensitivity of the LIF technique for various species. This is developed as a part of high enthalpy flow diagnostics and will be useful to elucidate the rotational and vibrational temperatures. Adequate sensitivity for the detection of O(2) and NO is demonstrated. Proposed improvements of the existing system are presented. Arepalli, Sivaram Unspecified Center NAS9-17900...