Download or read book Label free and Multi parametric Monitoring of Cell based Assays with Substrate embedded Sensors written by Maximilian Oberleitner and published by Springer. This book was released on 2017-09-26 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research. The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design principles and elucidates the boundary conditions of the new sensors.
Download or read book Biological Transformation written by Reimund Neugebauer and published by Springer Nature. This book was released on 2020-09-11 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: The global population is expected to rise to 9.8 billion by the year 2050 - with everyone ultimately striving for prosperity. New methods must therefore be found to achieve more efficient production. Research to date shows that the biological inventory that has evolved: its products, processes, principles and tools, can spur modern technology. The development of technological innovations based on biological concepts, with the goal of particularly innovative and sustainable value creation, today is collectively known as "biological transformation". It results in highly functional products with striking properties that can be both manufactured and utilized in a resource-saving way. In terms of taking responsibility of the good of all people, biological transformation is therefore a path that applied research will have to take. The Fraunhofer-Gesellschaft has recognized the developmental technology potential of biological transformation and sees it as its task not only to drive the relevant research forward, but also to promote public awareness of the topic.
Download or read book Label Free Monitoring of Cells in vitro written by Joachim Wegener and published by Springer Nature. This book was released on 2019-11-18 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.
Download or read book Cell based Biosensors written by Qingjun Liu and published by Artech House. This book was released on 2009-10 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by recognized experts the field, this leading-edge resource is the first book to systematically introduce the concept, technology, and development of cell-based biosensors. You find details on the latest cell-based biosensor models and novel micro-structure biosensor techniques. Taking an interdisciplinary approach, this unique volume presents the latest innovative applications of cell-based biosensors in a variety of biomedical fields. The book also explores future trends of cell-based biosensors, including integrated chips, nanotechnology and microfluidics. Over 140 illustrations help clarify key topics throughout the book.
Download or read book Imaging Flow Cytometry written by Natasha S. Barteneva and published by Humana. This book was released on 2015-11-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Download or read book Electrochemical Biosensors written by Serge Cosnier and published by CRC Press. This book was released on 2015-01-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy
Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.
Download or read book Mammalian Toxicology written by Mohamed Abou-Donia and published by John Wiley & Sons. This book was released on 2015-05-04 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mammalian Toxicology surveys chemical agents and examines how such chemicals impact on human health, emphasizing the importance in minimizing environmental exposure to chemical and physical hazards in our homes, communities and workplaces through such media as contaminated water, soil and air. Starting with the basic principles on a wide range of toxic agents, this textbook describes how they enter the body, their mechanisms of action once inside, and strategies for diagnosis, prevention and treatment. Topics covered include: General principles of toxicology: pharmacological and toxicological principles underpinning the study of toxicology, risk assessments and mechanisms of cell death Disposition: routes of chemical exposures, entry into the body and various tissues, storage, metabolic biotransformation and elimination, with examples from various toxicants. Toxic agents: the occurrences, disposition in the body, health effects, toxic mechanisms, antidotes and treatments of a range of agents including pesticides, metals, solvents, gases, nanomaterials, food components and additives, pharmaceuticals, drugs of abuse, natural toxins, endocrine disruptors, radiation, and warfare weapons. Toxic effects: including neurotoxicity, developmental toxicity, immunotoxicity, teratogenecity, male and female reproductive toxicity, mutagenecity, carcinogenicity, pulmonary toxicity, cardiovascular toxicity, hepatotoxicity, gastrointestinal toxicity and cardiovascular toxicity Toxicology and society: epidemiological studies of chemical-induced diseases in human populations, and a vision for toxicology in the 21st century. Mammalian Toxicology is an essential primer for students of toxicology, biochemistry, biology, medicine and chemistry. It is also appropriate for professional toxicologists in research or regulatory affairs, and anyone who needs to understand the adverse effects of toxic agents on the human body.
Download or read book Advances in Cell Culture written by and published by . This book was released on 1989 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electric Cell Substrate Impedance Sensing and Cancer Metastasis written by Wen G. Jiang and published by Springer Science & Business Media. This book was released on 2012-09-12 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on this technology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.
Download or read book Handbook of Surface Plasmon Resonance written by Richard B. M. Schasfoort and published by Royal Society of Chemistry. This book was released on 2017-05-30 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events, this book provides a total system description including optics, fluidics and sensor surfaces for a wide researcher audience.
Download or read book Laser induced Graphene written by Ruquan Ye and published by . This book was released on 2020-11-30 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: LIG is a revolutionary technique that uses a common CO2 infrared laser scriber, like the one used in any machine shop, for the direct conversion of polymers into porous graphene under ambient conditions. This technique combines the preparation and patterning of 3D graphene in a single step, without the use of wet chemicals. The ease in the structural engineering and excellent mechanical properties of the 3D graphene obtained have made LIG a versatile technique for applications across many fields. This book compiles cutting-edge research on LIG by different research groups all over the world. It discusses the strategies that have been developed to synthesize and engineer graphene, including controlling its properties such as porosity, composition, and surface characteristics. The authors are pioneers in the discovery and development of LIG and the book will appeal to anyone involved in nanotechnology, chemistry, environmental sciences, and device development, especially those with an interest in the synthesis and applications of graphene-based materials.
Download or read book The Microflow Cytometer written by Frances S. Ligler and published by Pan Stanford Publishing. This book was released on 2010-05-31 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Great book! Excellent compilation. From history of the very early days of flow cytometers to the latest unique unconventional microflow cytometers. From commercialization philosophy to cutting edge engineering designs. From fluid mechanics to optics to electronic circuit considerations. Well balanced and comprehensive."--Shuichi Takayama University of Michigan, USA.
Download or read book Electroporation and Electrofusion in Cell Biology written by C.A. Jordan and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
Download or read book Fitting Models to Biological Data Using Linear and Nonlinear Regression written by Harvey Motulsky and published by Oxford University Press. This book was released on 2004-05-27 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most biologists use nonlinear regression more than any other statistical technique, but there are very few places to learn about curve-fitting. This book, by the author of the very successful Intuitive Biostatistics, addresses this relatively focused need of an extraordinarily broad range of scientists.
Download or read book Handbook of Cell Biosensors written by Gérald Thouand and published by Springer. This book was released on 2021-10-01 with total page 992 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook is an interdisciplinary and comprehensive reference covering all aspects of cell biosensors. It is divided into four main sections which are led and organized by numerous international experts. The scope of coverage includes: Fundamentals and genetics for biosensor applications Transducers, Materials and Systems Markets, innovation and education Application of biosensors in business Biosensor research is an exciting hybrid world where biologists, chemists, physicists, engineers and computer engineers come together. This handbook will serve as an invaluable living resource for all researchers in academia and industry working with cell biosensors.
Download or read book Biomedical Sensors written by Deric P. Jones and published by Momentum Press. This book was released on 2010 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sensors are the eyes, ears, and more, of the modern engineered product or system- including the living human organism. This authoritative reference work, part of Momentum Press's new Sensors Technology series, edited by noted sensors expert, Dr. Joe Watson, will offer a complete review of all sensors and their associated instrumentation systems now commonly used in modern medicine. Readers will find invaluable data and guidance on a wide variety of sensors used in biomedical applications, from fluid flow sensors, to pressure sensors, to chemical analysis sensors. New developments in biomaterials- based sensors that mimic natural bio-systems will be covered as well. Also featured will be ample references throughout, along with a useful Glossary and symbols list, as well as convenient conversion tables.
