Download or read book Isolation of Breast Tumor Suppressor Genes from Chromosome Lip written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have previously shown that chromosome lip 15.5 exhibits loss of heterozygosity (LOH) in 60% of breast tumors, and that there is a significant correlation between lip LOH, lymphatic invasion and aggressive metastatic disease. Our data suggests that chromosome lip 15.5 harbors a metastasis suppressor gene. An intriguing candidate gene that we have mapped to the metastasis suppressor locus on chromosome lip15.5 is integrin-linked kinase (ILK). ILK is a newly identified ankyrin-repeat containing serine/threonine kinase that binds to the cytoplasmic domains of both Beta 1 and the Beta 3 integrins. Cell-cell and cell-matrix interactions are important prerequisites of the metastatic process and appear to be modulated by cell adhesion receptors called integrins. There is a growing body of evidence suggesting that variations in the expression of these molecules can have a profound effect on tumor biology. In preliminary experiments, we have provided evidence that Integrin-linked kinase expression is down-regulated in primary breast tumors and in cell lines derived from metastatic breast tumors. We have shown that ILK overexpression inhibits the growth of the highly metastatic breast cancer cell line MDA-MB-435. In addition, ILK overexpression stimulates the levels of the growth suppressing integrin alpha5Beta1 and inhibits the levels of alphavBeta3, a growth promoting integrin. These studies suggest that ILK is a breast cancer metastasis suppressor gene.
Download or read book Isolation of Breast Tumor Suppressor Genes from Chromosome 11p written by Pratima Karnik and published by . This book was released on 2001 with total page 43 pages. Available in PDF, EPUB and Kindle. Book excerpt: In published studies, we have shown that chromosome 11p15.5 exhibits loss of heterozygosity (LOH) in ^60% of breast tumors, and that there is a significant correlation between lip LOH, lymphatic invasion and aggressive metastatic disease. Our data suggests that chromosome 11p15.5 harbors a tumor/metastasis suppressor gene. An intriguing candidate gene that we have mapped to the tumor/metastasis suppressor locus on chromosome 11p15.5 is Integrin-linked Kinase (ILK). ILK is a newly identified ankyrin-repeat containing serine/threonine kinase that binds to the cytoplasmic domains of both 81 and the 83 integrins. Here, we present evidence that the Integrin-Linked Kinase (ILK) gene maps to the commonly deleted chromosome 11p15.5 and suppresses malignant growth of human breast cancer cells both in vitro and in vivo. ILK is expressed in normal breast tissue but-not in metastatic breast cancer cell lines or in advanced breast cancers. Transfection of wild-type ILK into the MDA-MBA35 mammary carcinoma cells potently suppressed their growth and invasiveness in vitro, and reduced the cells' ability to induce tumors and metastasize in athymic mice. Conversely, expression of the ankyrin repeat or catalytic domain mutants of ILK failed to suppress the growth of these cells. Growth suppression by ILK is not due to apoptosis but is mediated by its ability to block cell cycle progression in the G1 phase. These findings directly demonstrate that ILK deficiency facilitates neoplastic growth and suggest a novel role for the ILK gene in tumor suppression.
Download or read book Isolation of a Breast Cancer Tumor Suppressor Gene from Chromosome 3p written by and published by . This book was released on 1995 with total page 19 pages. Available in PDF, EPUB and Kindle. Book excerpt: Loss of tumor suppressor genes by genetic mechanisms represent critical molecular events in the development and progression of breast cancer. One or more of these tumor suppressor genes likely resides on the short arm of chromosome 3 (3p) and appears to be involved in nearly 50% of breast cancers. We have identified a region in 3pl4 which undergoes recurrent homozygous deletion or rearrangement in breast cancer cell lines. A set of cloned DNA molecules spanning the target region has been established and a gene search is underway. In order to develop a functional tumor suppressor test of the 3pl4 and other target regions, we have begun to modify specific YACs with the Neomycin resistance gene that will permit their selective retention in mammalian cells. The identification of this homozygous deletion region is an exciting development that has resulted in a modification of the order for our Specific Aims.
Download or read book Obzor raboty gorodskich bibliotek written by and published by . This book was released on 1967 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of Genes from Chromosome Region Ip31 Involved in the Development of Breast Cancer written by and published by . This book was released on 2001 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: The p31 region of chromosome 1 shows frequent (50%) loss of heterozygosity (LOH) in breast tumors. This observation implies the presence of a tumor suppressor gene in this region which, when mutated contributes to tumorigenesis. The maximal common region undergoing LOH extends over approximately 2Mbp. We have constructed a map of overlapping BAC clones spanning this region with only 4 gaps. The total available sequence from this region suggests that approximately 50-60% of it is now completed. Using gene analysis tools, we have been able to demonstrate that few full-length genes are located in this region and that the ESTs from the databases are clustered to a proximal position of the contig. The high level of sequencing completed from this region means that it will soon be possible to establish a baseline transcription map. The genes identified in this way can now be tested systematically for mutations in breast tumors. The progesterone receptor gene lies at the limit of the region suggesting that it was possibly the target for LOH. We established the exon/intron structure of this gene and undertook SSCP analysis of breast tumors. No gene-inactivating mutations were found excluding this gene from involvement in breast cancer development.
Download or read book Isolation of Breast Tumor Supressor Genes from Chronosome 11p written by and published by . This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have previously shown that chromosome 11p15.5 exhibits loss of heterozygosity (LOH) in 60% of breast tumors, and that there is a significant correlation between 11p LOH, lymphatic invasion and aggressive metastatic disease. Our data suggests that chromosome 11p15.5 harbors a metastasis suppressor gene. An intriguing candidate gene that we have mapped to the metastasis suppressor locus on chromosome 11p15.5 is Integrin-linked kinase (ILK). ILK is a newly identified ankyrin-repeat containing serine/threonine kinase that binds to the cytoplasmic domains of both 131 and the 133 integrins.
Download or read book How Tobacco Smoke Causes Disease written by United States. Public Health Service. Office of the Surgeon General and published by . This book was released on 2010 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report considers the biological and behavioral mechanisms that may underlie the pathogenicity of tobacco smoke. Many Surgeon General's reports have considered research findings on mechanisms in assessing the biological plausibility of associations observed in epidemiologic studies. Mechanisms of disease are important because they may provide plausibility, which is one of the guideline criteria for assessing evidence on causation. This report specifically reviews the evidence on the potential mechanisms by which smoking causes diseases and considers whether a mechanism is likely to be operative in the production of human disease by tobacco smoke. This evidence is relevant to understanding how smoking causes disease, to identifying those who may be particularly susceptible, and to assessing the potential risks of tobacco products.
Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book MRCOG Part One written by Alison Fiander and published by Cambridge University Press. This book was released on 2016-10-13 with total page 557 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fully updated and illustrated handbook providing comprehensive coverage of all curriculum areas covered by the MRCOG Part 1 examination.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1828 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetics Abstracts written by and published by . This book was released on 1996 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Diagnostic and Therapeutic Advances in Pediatric Oncology written by David O. Walterhouse and published by Springer Science & Business Media. This book was released on 1998-01-31 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of Diagnostic and Therapeutic Advances in Pediatric Oncology for the Cancer Treatment and Research Series is to provide an up-to-date summary of how recent advances in cancer research are being applied to the care of children with solid tumors. The interface of cancer research with clinical practice in pediatric oncology has never been more intimate than today. While researchers are identifying oncogenes and tumor suppressor genes and are studying their specific functions, clinicians are using knowledge of oncogenes and tumor suppressor genes for diagnosing cancer in children, for therapeutic decision-making purposes, and for prognostic purposes. The first three chapters in this book describe models for understanding the causes of childhood cancer that were perhaps initially identified by clinicians and that are now being studied and understood by researchers. These chapters will describe research evidence that supports roles for the involvement of normal developmental regulatory genes in childhood oncogenesis, of abnormal immune regulation in childhood oncogenesis, and of heredity in childhood oncogenesis. The next eight chapters are devoted to descriptions of the appli cation of new research developments to clinical practice with reference to the most common forms of solid tumors of childhood outside the central nervous system. The final chapter will describe late effects of childhood cancer and its therapy and the impact research is having on understanding and perhaps preventing these late effects.
Download or read book Hereditary Effects of Radiation written by United Nations. Scientific Committee on the Effects of Atomic Radiation and published by The Committee. This book was released on 2001 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 2001 report completed a comprehensive review of the risks to offspring following parental exposure to radiation. The review included an evaluation of those diseases which have both hereditary and environmental components. The major finding is that the total hereditary risk to the first generation following radiation is less than one tenth of the risk of fatal carcinogenesis following irrradiation. The Committee concluded that a sounder basis now exists for estimating the hereditary risks of radiation exposure. This is due to advances in molecular genetics, and in the evaluation of multifactorial diseases, such as coronary heart disease.
Download or read book Leong s Manual of Diagnostic Antibodies for Immunohistology written by Runjan Chetty and published by Cambridge University Press. This book was released on 2016-11-28 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a unique A-Z guide to antibodies for immunohistology, this is an indispensable source for pathologists to ensure the correct application of immunohistochemistry in daily practice. Each entry includes commercial sources, clones, descriptions of stained proteins/epitopes, the full staining spectrum of normal and tumor tissues, staining pattern and cellular localization, the range of conditions of immunoreactivity, and pitfalls of the antibody's immunoprofile, giving pathologists a truly thorough quick-reference guide to sources, preparation and applications of specific antibodies. Appendices provide useful quick-reference tables of antibody panels for differential diagnoses, as well as summaries of diagnostic applications. Expanded from previous editions with over forty new entries, this handbook for diagnostic, therapeutic, prognostic and research applications of antibodies is an essential desktop book for practicing pathologists as well as researchers, residents and trainees.
Download or read book Oxford Textbook of Neurological Surgery written by Ramez Kirollos and published by Oxford University Press, USA. This book was released on 2019-09-05 with total page 1177 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neurosurgery is a rapidly developing and technically demanding branch of surgery that requires a detailed knowledge of the basic neuro-sciences and a thorough clinical approach. The Oxford Textbook of Neurological Surgery is an up-to-date, objective and readable text that covers the full scope of neurosurgical practice. It is part of the Oxford Textbooks in Surgery series, edited by Professor Sir Peter Morris. The book is split into 20 overarching sections (Principles of Neurosurgery, Neuro-oncology of Intrinsic Tumours; Extra-axial Tumours and Skull Lesions; Cerebro-Pontine Angle Tumours; Sellar and Supra-Sellar Tumours; Posterior Fossa Tumours; Pineal tumours; Uncommon Tumours and Tumour Syndromes; Neurotrauma and Intensive Care; Vascular Neurosurgery; Principles of Spinal Surgery; Spinal Pathology; Spinal Trauma; Peripheral Nerve Surgery; Functional Neurosurgery; Epilepsy; Paediatric Neurosurgery; Neurosurgery for Cerebrospinal Fluid Disorders and Neurosurgical Infection). Each section takes a dual approach with, 'Generic Surgical Management' chapters that focus on specific clinical problems facing the neurosurgeon (e.g. sellar/supra-sellar tumour, Intradural Spina Tumours etc.) and 'Pathology-Specific' chapters (e.g. Glioma, Meningeal Tumours, Scoliosis and Spinal Deformity, Aneurysm etc.). Where appropriate, this division provides the reader with easily accessible information for both clinical problems which present in a regional fashion and specific pathologies. The generic chapters cover aspects such as operative approaches, neuroanatomy and nuances. Specifically each chapter in the book incorporates several strands. Firstly the fundamental neuroscience (anatomy, pathology, genetics etc.) that underlies the clinical practice. Secondly, a review of the requisite clinical investigations (e.g. angiography, electrodiagnostics, radiology). Thirdly, a thorough evidence based review of clinical practice. Following this a consideration of the key debates and controversies in the field with 'pro-' and 'con-' sections (e.g. minimally invasive spine surgery, microsurgical treatment of aneurysms) is provided. A summary of the key papers and clinical scales relevant to neurosurgery form the concluding part. The book is a 'one-stop' text for trainees and consultants in neurosurgery, residents, those preparing for sub-specialty exams and other professionals allied to surgery who need to gain an understanding of the field. It acts as both a point of reference to provide a focussed refresher for the experienced neurosurgeon as well as a trusted training resource.
Download or read book Breast Diseases written by Radhakrishna Selvi and published by Springer. This book was released on 2014-12-05 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is challenging enough to be able to make a diagnosis in mammography, but the newer standards of care demand more than just providing a diagnosis. This book is an excellent resource to improve the understanding of breast diseases, recognize and manage problems encountered in breast imaging and clinical management of the breast diseases. With forty four chapters, this volume is divided into six sections on screening of breast cancer, imaging modalities, benign breast disorders, interventional procedures, pathological considerations and breast cancer. Breast Diseases: Imaging and Clinical Management is a crisp volume on clinical and multimodality breast imaging with emphasis on interventional procedures, pathology and the entire spectrum of breast cancers.
Download or read book Human Chromosomes written by Orlando J. Miller and published by Springer Science & Business Media. This book was released on 2011-06-28 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of this well-known text provides students, researchers and technicians in the area of medicine, genetics and cell biology with a concise, understandable introduction to the structure and behavior of human chromosomes. This new edition continues to cover both basic and up-to-date material on normal and defective chromosomes, yet is particularly strengthened by the complete revision of the material on the molecular genetics of chromosomes and chromosomal defects. The mapping and molecular analysis of chromosomes is one of the most exciting and active areas of modern biomedical research, and this book will be invaluable to scientists, students, technicians and physicians with an interest in the function and dysfunction of chromosomes.
