Download or read book Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry written by Bindesh Shrestha and published by Elsevier. This book was released on 2021-04-23 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. Provides the introductory foundations of imaging mass spectrometry for those new to the technique Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work Includes case studies summarizing the imaging MS techniques developed for the class of molecules
Download or read book MALDI Mass Spectrometry Imaging written by Tiffany Siegel Porta and published by Royal Society of Chemistry. This book was released on 2021-12-03 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.
Download or read book Separation Techniques in Analytical Chemistry written by Rudolf Bock and published by Walter de Gruyter GmbH & Co KG. This book was released on 2023-07-24 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: The separation of a mixture into its individual components is one of the most fundamental procedures in analytical and industrial chemistry. This classic book in analytical chemistry provides a comprehensive yet systematic outline of all known separation methods. Through its detailed treatment of the basic principles of separation possibilities, it not only covers what is currently known, but also represents a treasure trove of methods that are still awaiting further development. It is clearly structured and contains interesting examples, further reading and a detailed index. An indispensable book for advanced students of natural sciences (chemistry, biochemistry, food chemistry, pharmacy, clinical chemistry, environmental sciences) and technology (chemical engineering, chemical-physical measurement & biotechnology), as well as teachers of these disciplines.
Download or read book Imaging Mass Spectrometry written by Laura M Cole and published by Springer Nature. This book was released on 2023-07-06 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Download or read book Methodological Advances of MALDI Mass Spectrometry Based Techniques in Organic and Biomedical Analysis written by Cheng Guo and published by Frontiers Media SA. This book was released on 2022-07-13 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Multimodal Spectral Microscopy and Imaging Mass Spectrometry of Biomolecules in Cells and Tissues written by Yang Xu and published by . This book was released on 2012 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomolecular imaging enables localization and characterization of biomolecules at the cellular and tissue levels in order to better understand their roles in biological organisms. Although a plethora of imaging techniques is available, there is a need to improve current imaging methodologies and apply them for selective analyses of complex biological specimens. The work described in this thesis is related to development and applications of spectral imaging and imaging mass spectrometry (IMS) methodologies. Spectral imaging uses either labeling dyes or native chromophores to visualize biomolecules in cells and tissues under a specialized light microscope, while IMS provides direct detection and analysis of biomolecules on tissue surfaces with preserved spatial distribution. In this initial study, multimodal spectral imaging of cells, cellular organelles, and tissues was performed on a light microscope upon the addition of a transmission diffraction grating. The instrument concurrently recorded spectral images by fluorescence, darkfield, brightfield, and differential interference contrast (DIC) spectral microscopy. Fluorescence signals originating from fluorescently labeled biomolecules in cells were collected through triple or single filter cubes, separated by the grating, and were imaged using a charge-coupled device (CCD) camera. Cellular components such as nuclei, cytoskeleton, and mitochondria were spatially separated by the fluorescence spectra of the fluorophores present in them, providing detailed multi-colored spectral images of cells. Additionally, the grating-based spectral microscope enabled measurement of scattering and absorption spectra of unlabeled cells and stained tissue sections using darkfield and brightfield spectral microscopy, respectively. In the second project, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) was utilized to visualize the protein spatial distribution on tissue surfaces. Our study has been focused on imaging of proteins in kidney tissue sections originating from apparently healthy (normal) mice and mice with polycystic kidney disease (PKD). Reconstruction of protein ion maps and identification of proteins by on-tissue enzymatic digestion were performed. MALDI-MS images were correlated with histological features obtained by hematoxylin and eosin staining, providing localization of peptides in different parts of the kidney (renal pelvis, medulla, and cortex). Nano-HPLC was coupled with imaging mass spectrometry to identify proteins in kidney tissue sections. Statistical analyses such as principle component analysis (PCA) was employed to achieve tissue classification and differentiate between healthy and diseased tissues. Experiments are underway to discover and validate proteins that can become potential biomarkers of PKD. In summary, we demonstrated that fluorescence, scattering, and absorption spectral images of cells can be acquired by incorporation of a transmission diffraction grating into a light microscope. The present spectral imaging methodology provides a readily affordable approach for multimodal spectral characterization of biological cells and other specimens. A reproducible MALDI-IMS workflow was established and resulted in protein mass distribution maps of normal and PKD kidney tissues. Statistical comparison of MALDI-MS images provides classification of healthy and diseased tissues. Several proteins were localized in tissues and their identity will be further confirmed by HPLC combined with tandem mass spectrometry (MS/MS).
Download or read book Mass Spectrometry Imaging of Small Molecules written by Lin He and published by Humana. This book was released on 2016-09-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents new developments in the field of mass spectrometry imaging, covering imaging, software, data analysis, new instrumentations, and new methodological approaches. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification and from data collection to visualization of small molecule mapping in complex samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.
Download or read book Detection of Chemical Biological Radiological and Nuclear Agents for the Prevention of Terrorism written by Joseph Banoub and published by Springer. This book was released on 2014-11-03 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: This NATO-ASI installment is designed to provide an advanced overview for doctoral and post-doctoral candidates of the state-of-the-art technologies for bio-detection. The main objective of the work aims at providing readers with the latest developments necessary to successfully understand the CBRN Agents and their associated biotechnologies. The core methods focused on are mass spectrometry (including chromatographic and electrophoretic separation) and comparisons to spectroscopic, immunological and molecular analysis of chemical, biological and nuclear agents.
Download or read book Mass Spectrometry Imaging written by Stanislav S. Rubakhin and published by Humana Press. This book was released on 2012-10-13 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular BiologyTM series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.
Download or read book Bioinformatics and Biomedical Engineering written by Ignacio Rojas and published by Springer Nature. This book was released on 2022-06-07 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume constitutes the proceedings of the 9th International Work-Conference on IWBBIO 2020, held in Maspalomas, Gran Canaria, Spain, in June 2022. The total of 75 papers presented in the proceedings, was carefully reviewed and selected from 212 submissions. The papers cover the latest ideas and realizations in the foundations, theory, models, and applications for interdisciplinary and multidisciplinary research encompassing disciplines of computer science, mathematics, statistics, biology, bioinformatics, and biomedicine.
Download or read book Biocomputing 2007 written by and published by World Scientific. This book was released on 2006-12-11 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Pacific Symposium on Biocomputing (PSB) 2007 is an international, multidisciplinary conference for the presentation and discussion of current research in the theory and application of computational methods in problems of biological significance. Presentations are rigorously peer reviewed and are published in an archival proceedings volume. PSB 2007 will be held January 3-7, 2007 at the Grand Wailea, Maui. Tutorials will be offered prior to the start of the conference.PSB 2007 will bring together top researchers from the US, the Asian Pacific nations, and around the world to exchange research results and address open issues in all aspects of computational biology. It is a forum for the presentation of work in databases, algorithms, interfaces, visualization, modeling, and other computational methods, as applied to biological problems, with emphasis on applications in data-rich areas of molecular biology.The PSB has been designed to be responsive to the need for critical mass in sub-disciplines within biocomputing. For that reason, it is the only meeting whose sessions are defined dynamically each year in response to specific proposals. PSB sessions are organized by leaders of research in biocomputing's “hot topics.” In this way, the meeting provides an early forum for serious examination of emerging methods and approaches in this rapidly changing field.
Download or read book Mass Spectrometry Imaging of Small Molecules written by Young-Jin Lee and published by Humana. This book was released on 2021-12-14 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents updated methods and new developments in the field of mass spectrometry imaging. Chapters guide readers through four parts covering imaging, software, data analysis, new instrumentation, and new methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to be a useful practical guide to researchers to help further their study in this field.
Download or read book Introduction to Mass Spectrometry written by J. Throck Watson and published by John Wiley & Sons. This book was released on 2007-11-12 with total page 852 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised and updated, this text provides an easy-to-read guide to the concept of mass spectrometry and demonstrates its potential and limitations. Written by internationally recognised experts and utilising "real life" examples of analyses and applications, the book presents real cases of qualitative and quantitative applications of mass spectrometry. Unlike other mass spectrometry texts, this comprehensive reference provides systematic descriptions of the various types of mass analysers and ionisation, along with corresponding strategies for interpretation of data. The book concludes with a comprehensive 3000 references. This multi-disciplined text covers the fundamentals as well as recent advance in this topic, providing need-to-know information for researchers in many disciplines including pharmaceutical, environmental and biomedical analysis who are utilizing mass spectrometry
Download or read book Introduction to Mass Spectrometry of Biomolecules written by Marko Jovanović and published by Nova Science Publishers. This book was released on 2016 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: The theses, lectures and solved problems within this book give an introduction to principles of mass spectrometry related to the analysis of biomolecules. The book focuses on MS and MS/MS analysis of peptides, oligosaccharides, lipids, glycoconjugates and polyphenols. The basic properties of modern MS ionization techniques and mass analyzers are presented, as well as appropriate MS methods for each class of biomolecules in modern biomedical scientific research. The biggest part of this book is focused on MS/MS analysis of biomolecules: how the MS and MS/MS data are generated, what are the basic strategies for their structural analysis, as well as how to interpret the respective MS and MS/MS data. This book also covers principles and examples of advanced MS/MS analyses, serving as a platform towards an intermediate level of understanding scientific research carried out in the field of structural MS analysis of biomolecules. It is the author's hope that the reader will be able to understand principles of MS analyses related to their scientific and/or analytical interest, as well as design/help to propose MS and MS/MS experiments, as well as interpret the obtained data. This book is primarily written for students with basic knowledge of general, organic and analytical chemistry, and who are interested in applying MS-based methodologies in biomedical research fields, as well as interested in pursuing a career in mass spectrometry. Therefore it is best suited for senior undergraduate students and first/second year Ph.D. students. However, since MS instrumentation is rapidly adopted on all levels of scientific as well as analytical laboratories, the book should prove useful to scientists -- as well as analysts -- interested in applying MS in their upgraded laboratories. The author hopes that the reader, after studying the content presented, will be able to read primary scientific literature containing MS data to learn more about specific topics of interest, as well as be more capable of choosing a MS-related career path. For senior scientists, this book can offer some basic insights related to choosing a post-doc laboratory, or simply easier and more fruitful cooperation with mass spectrometrists.
Download or read book Introduction to Protein Mass Spectrometry written by Pradip K. Ghosh and published by Elsevier. This book was released on 2024-04-26 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to Protein Mass Spectrometry, Second Edition provides a comprehensive overview of this increasingly important, yet complex, analytical technique. This book enables readers to understand how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. This new edition is fully updated to include the latest developments in the field and will feature new content covering recent progress in the areas where there have been the most exciting advances. These include PNNL’s multilevel-PCB-based SLIM realization, SLIM-Agilent QQQ field trials; employment of SLIM-IMS-cryo-IR combination in molecular structure determination; proximity-labelling mass spectrometry, and applications in neuroscience. Offers up-to-date, introductory information for scientists and researchers new to the field, as well as advanced insights into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly used MS instruments from a range of key manufacturers/developers, including Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, Waters and PNNL Includes biological applications and exploration of analytical tools and databases for bioinformatics Features definitions, case studies, and recent developments in protein mass spectrometry Includes sections new to this edition on SLIM (Structures for Lossless Ion Manipulation) and mass spectrometry applications in neuroscience, including synaptic biology and Alzheimer’s disease
Download or read book Cholesterol Modulation of Protein Function written by Avia Rosenhouse-Dantsker and published by Springer. This book was released on 2019-01-16 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems.
Download or read book The Power of Mass Spectrometry Imaging in Pharmaceutical Development written by Jillian Kailey Johnson and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry has emerged as a powerful tool for analyzing and identifying biomolecules of interest, including small molecules, pharmaceutical compounds, and tissue biomarkers - such as proteins and glycans. While most mass spectrometry based analytical techniques focus on liquid-chromatography coupled with tandem mass spectrometry for biomolecule analysis, mass spectrometry imaging (MSI) provides a unique advantage to map spatial distribution within an anatomical context of the tissue of interest. We use a high-resolution accurate mass MALDI Orbitrap LTQ XL mass spectrometer, that offers enhanced mass spectral resolution and high accuracy mass measurements critical for molecular identification, to perform MSI on thin tissue sections. In this thesis, I will highlight specific examples where the information gained from imaging is critical to understanding the question of interest: 1) Development of pathology-directed biomarker analysis of clinically relevant tissues 2) Pushing throughput for MSI in vitro drug penetration analysis of three-dimensional cell cultures including spheroid and organoid systems and 3) Analysis of lipids in insulator regions of the electric eel for the development of bioelectric batteries.