Download or read book Integration and Excision of DNA Molecules written by P.H. Hofschneider and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topic of this years' ~osbach Colloquium was DNA integration. We have tried to bring together experts from different fields of research who are studying natural processes by which DNA molecules from differ ent sources are linked. It has been known for a long time that such linkage occurs between the chromosomes of bacteriophages and plasmids on the one hand and the chromosome of the bacterial host on the other. This process has been especially well studied in bacteriophage A. Since it is controlled in a complicated way, we began with a lecture by M. ptashne on these regulatory processes. H. Nash described the inte gration of bacteriophage A into the bacterial chromosome. To put this site-specific process into perspective, G. Mosig lectured on genetic recombination in prokaryotes in general and K. Murray described the use of bacteriophage A as an artificial vector for genetic engineering. A different kind of bacteriophage integration is shown by bacteriophage Mu, which is much less specific in its choice of an integration site than A. The properties of this phage were described by P. van de Putte.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Life Sciences written by Robert D. Wells and published by Springer. This book was released on 2018-01-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Life Sciences: An Encyclopedic Reference will focus on understanding biological phenomena at the level of molecules and their interactions that govern life processes. The work will include articles on genes and genomes, protein structure and function, systems biology using genomics and proteomics as the focus, molecular aspects of cell structure and function, unifying concepts and theories from biology, chemistry, mathematics and physics that are essential for understanding the molecular life sciences (including teaching perspectives and assessment tools), and basic aspects of the various experimental approaches that are used in the Molecular Life Sciences.
Download or read book Mobile DNA III written by Michael Chandler and published by John Wiley & Sons. This book was released on 2020-07-24 with total page 1347 pages. Available in PDF, EPUB and Kindle. Book excerpt: An exploration of the raw power of genetic material to refashion itself to any purpose... Virtually all organisms contain multiple mobile DNAs that can move from place to place, and in some organisms, mobile DNA elements make up a significant portion of the genome. Mobile DNA III provides a comprehensive review of recent research, including findings suggesting the important role that mobile elements play in genome evolution and stability. Editor-in-Chief Nancy L. Craig assembled a team of multidisciplinary experts to develop this cutting-edge resource that covers the specific molecular mechanisms involved in recombination, including a detailed structural analysis of the enzymes responsible presents a detailed account of the many different recombination systems that can rearrange genomes examines the tremendous impact of mobile DNA in virtually all organisms Mobile DNA III is valuable as an in-depth supplemental reading for upper level life sciences students and as a reference for investigators exploring new biological systems. Biomedical researchers will find documentation of recent advances in understanding immune-antigen conflict between host and pathogen. It introduces biotechnicians to amazing tools for in vivo control of designer DNAs. It allows specialists to pick and choose advanced reviews of specific elements and to be drawn in by unexpected parallels and contrasts among the elements in diverse organisms. Mobile DNA III provides the most lucid reviews of these complex topics available anywhere.
Download or read book Clostridia written by Nigel P. Minton and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: To the uninitiated, the genus Clostridium is likely more to be associated with disease than biotechnology. In this volume, we have sought to remedy this misconception by compiling aseries of chapters which, together, provide a practically-oriented handbook of the biotechnologie potential of the genus. Clostridium is a broad grouping of organisms that together undertake a myriad of biocatalytic reactions. In the first two chapters, the reader is introduced to this diversity, both taxonomically and physiologically. In the following chapter, the current state of genetic analysis of members of the genus is reviewed. The remaining chapters concentrate on specific, exploit able aspects of individual Clostridium species-highlighting their range of unique capabilities (of potential or recognized industrial value), particu larly in the areas of biotransformation, enzymology, and the production of chemical fuels. Fittingly, the final chapter demonstrates that even the most toxic of the clostridia can be of therapeutic value. The contributors to this volume reflect the trans national interest in Clostridium, and we are indebted to each of them for making this volume possible. We particularly wish to acknowledge the contributions, both to this volume and to microbiology in general, of Dr. Elizabeth Cato, who, sadly, died shortly be fore publication ofthis volume. Finally, we would like to join the authors in recommending closer and wider consideration of the attributes and capabilities of this genus.
Download or read book Molecular Genetic and Statistical Techniques for Behavioral and Neural Research written by Robert T. Gerlai and published by Academic Press. This book was released on 2018-04-24 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior, a critical piece of the puzzle for clinicians, scientists, course instructors and advanced undergraduate and graduate students. Chapters examine neuroinformatics, genetic and neurobehavioral databases and data mining, also providing an analysis of natural genetic variation and principles and applications of forward (mutagenesis) and reverse genetics (gene targeting). In addition, the book discusses gene expression and its role in brain function and behavior, along with ethical issues in the use of animals in genetics testing. Written and edited by leading international experts, this book provides a clear presentation of the frontiers of basic research as well as translationally relevant techniques that are used by neurobehavioral geneticists. - Focuses on new techniques, including electrocorticography, functional mapping, stereo EEG, motor evoked potentials, optical coherence tomography, magnetoencephalography, laser evoked potentials, transmagnetic stimulation, and motor evoked potentials - Presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior - Written and edited by leading international experts
Download or read book Introduction to Pharmaceutical Biotechnology Volume 1 Second Edition Basic Techniques and Concepts written by and published by . This book was released on 2024-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology written by Nancy Craig and published by Oxford University Press. This book was released on 2014-05 with total page 945 pages. Available in PDF, EPUB and Kindle. Book excerpt: The biological world operates on a multitude of scales - from molecules to tissues to organisms to ecosystems. Throughout these myriad levels runs a common thread: the communication and onward passage of information, from cell to cell, from organism to organism and ultimately, from generation to generation. But how does this information come alive to govern the processes that constitute life? The answer lies in the molecular components that cooperate through a series of carefully-regulated processes to bring the information in our genome to life. These components and processes lie at the heart of one of the most fascinating subjects to engage the minds of scientists today: molecular biology. Molecular Biology: Principles of Genome Function, Second Edition, offers a fresh approach to the teaching of molecular biology by focusing on the commonalities that exist between the three kingdoms of life, and discussing the differences between the three kingdoms to offer instructive insights into molecular processes and components. This gives students an accurate depiction of our current understanding of the conserved nature of molecular biology, and the differences that underpin biological diversity. Additionally, an integrated approach demonstrates how certain molecular phenomena have diverse impacts on genome function by presenting them as themes that recur throughout the book, rather than as artificially separated topics As an experimental science, molecular biology requires an appreciation for the approaches taken to yield the information from which concepts and principles are deduced. Experimental Approach panels throughout the text describe research that has been particularly valuable in elucidating difference aspects of molecular biology. Each panel is carefully cross-referenced to the discussion of key molecular biology tools and techniques, which are presented in a dedicated chapter at the end of the book. Molecular Biology further enriches the learning experience with full-color artwork, end-of-chapter questions and summaries, suggested further readings grouped by topic, and an extensive glossary of key terms. Features: A focus on the underlying principles of molecular biology equips students with a robust conceptual framework on which to build their knowledge An emphasis on their commonalities reflects the processes and components that exist between bacteria, archae, and eukaryotes Experimental Approach panels demonstrate the importance of experimental evidence by describing research that has been particularly valuable in the field
Download or read book Molecular Genetics of Plant Microbe Interactions written by Desh Pal S Verma and published by Springer Science & Business Media. This book was released on 1986-12-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Increased interest in the basic biology of plants and microorganisms stems from the fact that crop productivity is directly affected by plant-microbe interactions. In spite of the fact that plants exist in the environment amongst diverse species of microorganisms, only a few ever establish a direct relationship. Emerging awareness concerning the indirect effect of microbial association on plant growth and the possibility of using one microbe against another for controlling pathogenic interactions is at the genesis of new fields of studies. The primary reason for a microbe to associate with· photoautotrophic organisms (plants) is to tap its nutritional requirements, fixed carbon, as a source of energy. By hook or by crook, a microbe must survive. Some have evolved mechanisms to exploit plants to develop a niche for their biotropic demands. When in contact with a living plant, microorganisms may live in a passive association using exudates from the plant, invade it pathogenically or coexist with it in symbiosis. The plant responds to the interloper, either reacting in a hypersensitive manner to contain the invasion of pathogens, or by inducing a set of genes that leads toward symbiosis, or by simply succumbing to the invader. Thus, prior to contact wi th the plant, mic roorganism is able to sense the presence of the host and activate accordingly a set of genes required for the forthcoming interaction, whether symbiotic or pathogenic.
Download or read book Agrobacterium From Biology to Biotechnology written by Tzvi Tzfira and published by Springer Science & Business Media. This book was released on 2007-12-25 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
Download or read book Maintenance of Genome Integrity DNA Damage Sensing Signaling Repair and Replication in Plants written by Alma Balestrazzi and published by Frontiers Media SA. This book was released on 2016-05-06 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt: Environmental stresses and metabolic by-products can severely affect the integrity of genetic information by inducing DNA damage and impairing genome stability. As a consequence, plant growth and productivity are irreversibly compromised. To overcome genotoxic injury, plants have evolved complex strategies relying on a highly efficient repair machinery that responds to sophisticated damage perception/signaling networks. The DNA damage signaling network contains several key components: DNA damage sensors, signal transducers, mediators, and effectors. Most of these components are common to other eukaryotes but some features are unique to the plant kingdom. ATM and ATR are well-conserved members of PIKK family, which amplify and transduce signals to downstream effectors. ATM primarily responds to DNA double strand breaks while ATR responds to various forms of DNA damage. The signals from the activated transducer kinases are transmitted to the downstream cell-cycle regulators, such as CHK1, CHK2, and p53 in many eukaryotes. However, plants have no homologue of CHK1, CHK2 nor p53. The finding of Arabidopsis transcription factor SOG1 that seems functionally but not structurally similar to p53 suggests that plants have developed unique cell cycle regulation mechanism. The double strand break repair, recombination repair, postreplication repair, and lesion bypass, have been investigated in several plants. The DNA double strand break, a most critical damage for organisms are repaired non-homologous end joining (NHEJ) or homologous recombination (HR) pathway. Damage on template DNA makes replication stall, which is processed by translesion synthesis (TLS) or error-free postreplication repair (PPR) pathway. Deletion of the error-prone TLS polymerase reduces mutation frequencies, suggesting PPR maintains the stalled replication fork when TLS is not available. Unveiling the regulation networks among these multiple pathways would be the next challenge to be completed. Some intriguing issues have been disclosed such as the cross-talk between DNA repair, senescence and pathogen response and the involvement of non-coding RNAs in global genome stability. Several studies have highlighted the essential contribution of chromatin remodeling in DNA repair DNA damage sensing, signaling and repair have been investigated in relation to environmental stresses, seed quality issues, mutation breeding in both model and crop plants and all these studies strengthen the idea that components of the plant response to genotoxic stress might represent tools to improve stress tolerance and field performance. This focus issue gives researchers the opportunity to gather and interact by providing Mini-Reviews, Commentaries, Opinions, Original Research and Method articles which describe the most recent advances and future perspectives in the field of DNA damage sensing, signaling and repair in plants. A comprehensive overview of the current progresses dealing with the genotoxic stress response in plants will be provided looking at cellular and molecular level with multidisciplinary approaches. This will hopefully bring together valuable information for both plant biotechnologists and breeders.
Download or read book CRISPR Cas Systems written by Rodolphe Barrangou and published by Springer Science & Business Media. This book was released on 2012-12-13 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.
Download or read book Molecular Genetics of Recombination written by Andrés Aguilera and published by Springer Science & Business Media. This book was released on 2007-04-24 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work offers a fascinating insight into a crucial genetic process. Recombination is, quite simply, one of the most important topics in contemporary biology. This book is a totally comprehensive treatment of the subject, summarizing all existing views on the topic and at the same time putting them into context. It provides in-depth and up-to-date analysis of the chapter topics, and has been written by international experts in the field.
Download or read book The Dynamic Bacterial Genome written by Peter Mullany and published by Cambridge University Press. This book was released on 2005-09-26 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book provides an in-depth analysis of the mechanisms and biological consequences of genome rearrangements in bacteria. Each chapter examines the mechanisms involved in genome rearrangements and the direct biological consequences of these events. Because genome rearrangements are so important in evolution, at least one of the chapters views the phenomenon from an evolutionary angle. This book provides the reader with a holistic view of genome rearrangements (i.e., studies on both the biological consequences of genome rearrangement and the mechanisms underlying these processes are presented)." "The book is written by leading research workers in the field and is aimed at final-year undergraduates, postgraduate and postdoctoral workers, and established biologists."--BOOK JACKET.
Download or read book Recombinant DNA Technology written by Siddra Ijaz and published by Cambridge Scholars Publishing. This book was released on 2019-08-13 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.
Download or read book The Bacteriophage Lambda written by Alfred Day Hershey and published by . This book was released on 1971 with total page 810 pages. Available in PDF, EPUB and Kindle. Book excerpt:
