Download or read book Influence of Multivalent Metal Cations on Carbohydrate Separations and Fragmentation Patterns Via Ion Mobility Mass Spectrometry written by Kelsey Anne Morrison and published by . This book was released on 2019 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrates, oligosaccharides, and glycans occupy a corner of biopolymers that can be overlooked in the shadow of proteins and nucleic acids but are nonetheless essential for the survival of biological organisms. However, unlike other biological macromolecules, known carbohydrate units have largely homogeneous elemental compositions, where species are instead distinguished by variations in stereochemistry and branching patterns. Because this creates a scenario where complex biological samples may contain isomeric mixtures which cannot be differentiated by mass isolation, incorporating ion mobility separations in front of mass spectrometry can provide another dimension of information to distinguish among isomers. Yet another angle for enhancing glycan analysis with ion mobility-mass spectrometry is by leveraging interactions between carbohydrate species and metal cations, which can form adducts that have altered gas-phase structures relative to protonated or deprotonated ions. In some situations, metal adduction exaggerates the differences in effective size among isomers and thereby facilitates isomeric glycan separation in the drift time space.This dissertation is focused on conveying the utility of metal-glycan interactions for improving the ion mobility-mass spectrometric analysis of carbohydrate species while also describing some complementary experiments that were pursued in support of glycan analysis. Of note, ultraviolet photodissociation (UVPD) with metal-enhanced fragmentation and Fourier multiplexing of ion mobility-ion trap mass spectrometry contributed well to isomeric mixture analysis. Divalent metal cations including select transition metals and alkaline earth metals were evaluated for their potential to enhance drift time separations, with transition metals proving particularly useful in this regard for mixture analysis. Preliminary oligosaccharide analysis with divalent metal species found that carbohydrates will form both singly-charged monomeric metal-glycan adducts as well as doubly-charged dimeric metal-glycan adducts where both appear at the same m/z value. These dimers were subsequently scrutinized for the potential to form heterodimeric metal-glycan dimers wherein two different isomeric glycans could form a single dimer during a mixture analysis without mobility separation and form chimeric mass spectra. Through a combination of isotopic labeling and ion mobility-mass spectrometry, it was shown that heterodimeric metal-glycan species will form, and that mobility separation is vital for analyzing glycan mixtures.
Download or read book Assessing Isomeric Heterogeneity of Carbohydrates by Ion Mobility Mass Spectrometry IMMS written by Hongli Li and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: IMMS analysis is fast, sensitive, universal, is capable of resolving isomers and is able to provide unique mobility identities and valuable MS n data on the basis of mobility resolved species including precursor and product ions. Ultimately, IMMS could serve as a novel tool for high throughput identification of glycoprotein.
Download or read book Multidimensional Ion Mobility Spectrometry Coupled to Time of flight Mass Spectrometry written by Stormy Lee Ann Koeniger and published by . This book was released on 2006 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: For characterization of proteomic mixtures, high-quality primary sequence information is necessary to positively identify the components with database search algorithms. To improve parallel sequencing capabilities with this technology, a new drift tube design has been developed. This design provides high-efficiency fragmentation and improves the transmission efficiency of the fragments to the time-of-flight mass spectrometer. Additionally, the new method of collisional activation utilizes a system of potential at the exit of the drift tube, allowing rapid modulation of the potentials to provide precursor and fragment ion information during the course of a single LC separation.
Download or read book The Sugar Code written by Hans-Joachim Gabius and published by John Wiley & Sons. This book was released on 2011-09-19 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: A reader friendly overview of the structure and functional relevance of natural glycosylation and its cognate proteins (lectins), this book is also one of the few books to cover their role in health and disease. Edited by one of the pioneering experts in the field and written by a team of renowned researchers this resource is a perfect introduction for all students in life and medical sciences, biochemistry, chemistry and pharmacy. Website: WWW.WILEY-VCH.DE/HOME/THESUGARCODE
Download or read book Bioinformatics for Glycobiology and Glycomics written by Claus-Wilhelm von der Lieth and published by John Wiley & Sons. This book was released on 2009-10-01 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first to be dedicated to the bioinformatics of carbohydrates and glycoproteins. It provides an introduction to this emerging field of science both for the experimentalist working in glycobiology and glycomics, and also for the computer scientist looking for background information for the development of highly sophisticated algorithmic approaches. The book provides an overview of the state-of-the-art in the field, with reviews on databases, and the tools in use for analysis, interpretation, and prediction of the structures of complex carbohydrates, and demonstrates the value of bioinformatics for glycobiology. The availability of comprehensive databases and corresponding bioinformatics tools, to access and analyse the large amounts of experimental data relating to the structure of carbohydrates, will be a prerequisite for the success of the large-scale glycomics projects that aim to decipher new, so far unknown, biological functions of glycans. Efficient bioinformatics descriptions and tools can considerably enhance the efficiency of glycomics research, in terms of data quality, analysis and experimental costs. For a complete understanding of the molecular processes in which carbohydrates are involved, such as protein–carbohydrate interactions and the impact of glycosylation on protein function, knowledge of the 3D structure of the carbohydrate, the protein–carbohydrate complex, or the glycoprotein is often indispensable. This book provides a thorough introduction into methods used for conformational analysis of carbohydrates. Key features: Describes bioinformatic approaches to handle carbohydrate-active enzymes and glycosylation. Provides an overview on bioinformatics tools that facilitate analysis of carbohydrate structures. Gives introduction into molecular modelling of carbohydrate 3D structure and carbohydrates contained in the Protein Databank. Assumes only a basic knowledge of biology and bioinformatics.
Download or read book Analysis of Protein Post Translational Modifications by Mass Spectrometry written by John R. Griffiths and published by John Wiley & Sons. This book was released on 2016-11-07 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research
Download or read book Toxic Chemical and Biological Agents written by Giovanni Sindona and published by Springer Nature. This book was released on 2020-10-19 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book critically assesses the current state of knowledge on new and important detection technologies, e.g. mass spectrometry, tandem mass spectrometry, biosensor detection and tissue imaging, in connection with toxic chemical and biological agents. In general, the main topics discussed concern the risks and consequences of chemical and biological agents for human health in general, with special emphasis on all biochemical and metabolic pathways including the reproductive system. The exposome, genetic risks and the environment, various health hazard agents, risk assessment, environmental assessment and preparedness, and analysis of sub-lethal effects at the molecular level are also discussed. In closing, the book provides comprehensive information on the diagnosis of exposure, and on health concerns related to toxic chemical and biological agents.
Download or read book Proteomics Sample Preparation written by Jörg von Hagen and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
Download or read book Treatment of Micropollutants in Water and Wastewater written by Jurate Virkutyte and published by IWA Publishing. This book was released on 2010-08-14 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last few years there has been a growing concern over the increasing concentration of micropollutants originating from a great variety of sources including pharmaceutical, chemical engineering and personal care product industries in rivers, lakes, soil and groundwater. As most of the micropollutants are polar and persistent compounds, they are only partially or not at all removed from wastewater and thus can enter the environment posing a great risk to the biota. It is hypothesized that wastewater is one of the most important point sources for micropollutants. Treatment of Micropollutants in Water and Wastewater gives a comprehensive overview of modern analytical methods and will summarize novel single and hybrid methods to remove continuously emerging contaminants - micropollutants from the aqueous phase. New trends (e.g. sensor technology, nanotechnology and hybrid treatment technologies) are described in detail. The book is very timely because the new techniques are still in the development phase and have to be realized not only in the laboratory but also on a larger scale. The content of the book is divided into chapters that present current descriptive and analytical methods that are available to detect and measure micropollutants together with detailed information on various chemical, biological and physicochemical methods that have evolved over the last few decades. Treatment of Micropollutants in Water and Wastewater will also enable readers to make well informed choices through providing an understanding of why and how micropollutants must be removed from water sources, and what are the most appropriate and available techniques for providing a cost and technologically effective and sustainable solutions for reaching the goal of micropollutant-free water and wastewater. The book will be suitable for water and wastewater professionals as well for students and researchers in civil engineering, environmental engineering and process engineering fields.
Download or read book Differential Ion Mobility Spectrometry written by Alexandre A. Shvartsburg and published by CRC Press. This book was released on 2008-12-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.
Download or read book Chemistry and Significance of Condensed Tannins written by Richard W. Hemingway and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was developed from the proceedings of the first North American Tannin Conference held in Port. Angeles, Washington, August 1988. The objective of the conference was to bring together people with a common interest in condensed tannins and to promote interdisciplinary interactions that will lead to a better understanding of these important substances. Anot. her objective was the publicat. ion of this book because there has not been a monograph devoted to the chemistry and significance of tannins for several decades. The book is organized into sections dealing with the biosynthesis, structure, re actions, complexation with other biopolymers, biological significance, and use of tannins as specialty chemicals. The authors made a special attempt to focus on what we don't know as well as to provide a summary of what we do know in an effort to assist in planning future research. Our thanks go to the authors who so kindly contributed chapters and so pa tiently responded to our requests. We also thank Rylee Geboski and the Conference Assist. ance Staff, College of Forestry, Oregon State University, for their assistance in planning and conducting t. he conference, and Julia Wilson, Debbie Wolfe, Helen Coletka, and Nancy Greene of the Southern Forest Experiment Station, Pineville, Louisiana, who typed the chapt. ers. Linda Chalker-Scott was especially helpful in assisting us wit. h editing. Dick Hemingway is indebted t. o the staff of the Alexandria Forest.
Download or read book Sediments and Toxic Substances written by Wolfgang Calmano and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: In modern sediment research on contaminants five aspects are discussed which, in an overlapping succession, also reflect develop ment of knowledge on particle-associated pollutants during the past twenty-five years: (1) identification of sources and their distribution; (2) evaluation of solid/solution relations; (3) study of transfer mecha nisms to biological systems; (4) assessment of environment impact; and (5) selection and further development of remedial measures, in par ticular, of dredged materials. Scientific research and practical develop ment are still expanding in all these individual aspects. Similar to other waste materials, management of contaminated sed iments requires a holistic approach. This means that assessment of biogeochemical reactions, interfacial processes and transfer mecha nisms as well as the prognosis of long-term borderline conditions, in particular of capacity-controlling properties, should be an integrated part of the wider management scheme, i.e., the analytical and experi mental parameters should always be related to potential remediation options for a specific sediment problem. The underlying coordinated project, which was funded by the German Federal Ministry for Science and Technology (now the Federal Ministry for Education, Science, Research and Technology) provided excellent opportunities for multidisciplinary effort, bringing together biologists, chemists, engineers, geologists and other researchers. During its active phase, the group attracted much interest nationally and internationally. The group members highly appreciate the manifold contacts and invitations during the past five years.
Download or read book Allelopathy written by Manuel J. Reigosa and published by Springer Science & Business Media. This book was released on 2006-02-28 with total page 635 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are many good books in the market dealing with the subject of allelopathy. When we designed the outline of this new book, we thought that it should include as many different points of view as possible, although in an integrated general scheme. Allelopathy can be viewed from different of perspectives, ranging from the molecular to the ecosystem level, and including molecular biology, plant biochemistry, plant physiology, plant ecophysiology and ecology, with information coming also from the organic chemistry, soil sciences, microbiology and many other scientific disciplines. This book was designed to include a complete perspective of allelopathic process. The book is divided into seven major sections. The first chapter explores the international development of allelopathy as a science and next section deals with methodological aspects and it explores potential limitations of actual research. Third section is devoted to physiological aspects of allelopathy. Different specialists wrote about photosynthesis, cell cycle, detoxification processes, abiotic and biotic stress, plant secondary metabolites and respiration related to allelopathy. Chapters 13 through 16 are collectively devoted to various aspects of plant ecophysiology on a variety of levels: microorganisms, soil system and weed germination. Fundamental ecology approaches using both experimental observations and theoretical analysis of allelopathy are described in chapters 16 and 17. Those chapters deal with the possible evolutionary forces that have shaped particular strategies. In the section named “allelopathy in different environments”, authors primarily center on marine, aquatic, forest and agro ecosystems. Last section includes chapters addressing application of the knowledge of allelopathy.
Download or read book The Crustacean Nervous System written by Konrad Wiese and published by Springer Science & Business Media. This book was released on 2002 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique selection of reviews summarizes current knowledge in all major fields of crustacean neurobiology and all levels of their CNS organization, using lobster and crayfish. It not only imparts theoretical knowledge but also describes all available contemporary and advanced techniques, such as patch clamp recordings, microelectrode techniques, immunocytochemistry, and all methods of molecular genetics to identify cellular pathways of protein synthesis and peptidergic control. In summary, it is a comprehensive account of the research achievements in one of the major nervous systems besides the mammalian CNS.
Download or read book Nano and Bio Based Technologies for Wastewater Treatment written by Elvis Fosso-Kankeu and published by John Wiley & Sons. This book was released on 2019-05-21 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents recent challenges related to new forms of pollution from industries and discusses adequate state-of-the-art technologies capable to remediate such forms of pollution. Over the past few decades the boom in the industrial sector has contributed to the release in the environment of pollutants that have no regulatory status and which may have significant impact on the health of humans and animals. These pollutants also referred to as "emerging pollutants", are mostly aromatic compounds which derive from excretion of pharmaceutical, industrial effluents and municipal discharge. It is recurrent these days to find water treatment plants which no longer produce water that fits the purpose of domestic consumption based on newly established guidelines. This situation has prompted water authorities and researchers to develop tools for proper prediction and control of the dispersion of pollutants in the environment to ensure that appropriate measures are taken to prevent the occurrence of outbreaks due to sudden load of these pollutants in the water system. The chapters in this book cover a wide range of nano and bio-based techniques that have been designed for the real time detection of emerging contaminants in environmental water sources, geochemical models that are continuously improved for the prediction of inorganic contaminants migration from the mine solid wastes into ground and surface waters. Remediation strategies are also discussed and include effective techniques based on nanotechnology, advanced membrane filtration, oxidative and bio-degradation processes using various types of nanocatalysts, biocatalysts or supporting polymer matrices which are under advanced investigations for their implementation at large scale for the removal of recalcitrant pollutants from polluted water. Nano and Bio-Based Technologies for Wastewater Treatment: Prediction and Control Tools for the Dispersion of Pollutants in the Environment is divided is two sections. The first section covers the occurrence of emerging pollutants in environmental water while the second section covers state-of-the-art research on the removal of emerging pollutants from water using sustainable technologies. A total of 13 chapters addressing various topics related to the two sections are essentially based on recent developments in the respective field which could have a significant impact on the enhancement of the performance of wastewater treatment plants around the world, and especially in developing countries where access to clean and safe water remains a daily challenge.
Download or read book Nanotechnology and Biosensors written by Dimitrios P Nikolelis and published by Elsevier. This book was released on 2018-06-27 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology and Biosensors shows how nanotechnology is used to create affordable, mass-produced, portable, small sized biosensors to directly monitor environmental pollutants. In addition, it provides information on their integration into components and systems for mass market applications in food analysis, environmental monitoring and health diagnostics. Nanotechnology has led to a dramatic improvement in the performance, sensitivity and selectivity of biosensors. As metal-oxide and carbon nanostructures, gold and magnetite nanoparticles, and the integration of dendrimers in biosensors using nanotechnology have contributed greatly in making biosensors more effective and affordable on a mass-market level, this book presents a timely resource on the topic. Highlights nanotechnology-based approaches to the detection of enzyme inhibitors, direct enzymatic and microbial detection of metabolites, and nutrients using biosensors Includes examples on how nanotechnology has lead to improvements in the construction of portable, selective and sensitive biosensing devices Offers thorough coverage of biomarker/biosensor interaction for the rapid detection of toxicants and pollutants
Download or read book Polysaccharide Based Graft Copolymers written by Susheel Kalia and published by Springer Science & Business Media. This book was released on 2013-05-23 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Renowned experts give all essential aspects of the techniques and applications of graft copolymers based on polysaccharides. Polysaccharides are the most abundant natural organic materials and polysaccharide based graft copolymers are of great importance and widely used in various fields. Natural polysaccharides have recently received more attention due to their advantages over synthetic polymers by being non-toxic, biodegradable and available at low cost. Modification of polysaccharides through graft copolymerization improves the properties of polysaccharides. Grafting is known to improve the characteristic properties of the backbones. Such properties include water repellency, thermal stability, flame resistance, dye-ability and resistance towards acid-base attack and abrasion. Polysaccharides and their graft copolymers find extensive applications in diversified fields. Applications of modified polysaccharides include drug delivery devices, controlled release of fungicides, selective water absorption from oil-water emulsions, purification of water etc.
