Download or read book In Vitro Teratogenicity Testing Using a 3D Embryo like Gastruloid System written by Veronika Mantziou and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Comparison of In vitro 3D Human Embryoids with Current Models for Gastrulation written by Ajin Park and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gastrulation is an early morphogenetic process that is conserved across most metazoans and lays out the future body plan through the formation and shaping of the three germ layers: endoderm, mesoderm, and ectoderm. Despite its importance, not much is known about the events surrounding human gastrulation that occurs in utero due to ethical and technical limitations on studying human embryos. Therefore, many researchers have devised protocols for creating in vitro models of gastrulation using embryonic stem cells. Initially starting with mouse embryonic stem cells, the field of in vitro embryo models has advanced rapidly, with protocols using human embryonic stem cells being only recently published in 2020. However, validation of these models through comparison with other in vitro models or with animal gastrulae has only started. As such, this study investigates one of the first 3D gastruloid protocol using human embryonic stem cells to create an optimized protocol using the H1 embryonic stem cell line, which is then validated with immunofluorescent staining for germ layer markers. And lastly, cells from 3D gastruloids generated from H1 and RUES2-GRL cell lineswere tested for the ability to self-aggregate through a dissociation and reseeding experiment onto 2D micropatterns. Whereas cells from dissociated RUES2-GRL 3D gastruloids showed clear evidence of self-aggregation comparable to what was reported in a similar experiment with 2D gastruloids, cells from H1 3D gastruloids did not. However, further optimization of the protocol is needed. Overall, the results reported in this thesis have identified conditions to generate in vitro H1 3D elongating gastruloids with three germ layers, as well as showing the evolutionarily conserved sorting ability of 3D RUES2-GRL gastruloid cells.
Download or read book Scientific Frontiers in Developmental Toxicology and Risk Assessment written by National Research Council and published by National Academies Press. This book was released on 2000-11-21 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.
Download or read book Teratogenicity Testing written by Luís Félix and published by Humana. This book was released on 2019-06-27 with total page 614 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Teratogenicity Testing: Methods and Protocols describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.
Download or read book Pattern Formation and Dynamics in Nonequilibrium Systems written by Michael Cross and published by Cambridge University Press. This book was released on 2009-07-16 with total page 547 pages. Available in PDF, EPUB and Kindle. Book excerpt: An account of how complex patterns form in sustained nonequilibrium systems; for graduate students in biology, chemistry, engineering, mathematics, and physics.
Download or read book The Heritage of Experimental Embryology written by Viktor Hamburger and published by . This book was released on 1988 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here is a critical account of the experimental work of German biologist and Nobel laureate Hans Spemann, one of the founders of experimental embryology. The author, a distinguished developmental biologist, spent almost a decade in Spemann's laboratory. He examines Spemann's work and traces the different lines of investigation which emerged from his mentor's seminal research, and laid the foundation for modern cellular and developmental biology.
Download or read book The Science and Philosophy of the Organism written by Hans Driesch and published by . This book was released on 1908 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Salt Eaters written by Toni Cade Bambara and published by Vintage. This book was released on 2011-02-16 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: A community of Black faith healers witness an event that will change their lives forever in this "hard-nosed, wise, funny" novel (Los Angeles Times). Set in a fictional city in the American South, the novel also "inhabits the nonlinear, sacred space and sacred time of traditional African religion” (The New York Times Book Review). Though they all united in their search for the healing properties of salt, some of them are centered, some are off-balance; some are frightened, and some are daring. From the men who live off welfare women to the mud mothers who carry their children in their hides, the novel brilliantly explores the narcissistic aspect of despair and the tremendous responsibility that comes with physical, spiritual, and mental well-being.
Download or read book Embryos and Ancestors written by Sir Gavin De Beer and published by . This book was released on 1958 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mammalian Development written by Patrick P. L. Tam and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "A subject collection from Cold Spring Harbor perspectives in biology."
Download or read book Towards a Theory of Development written by Alessandro Minelli and published by Oxford University Press. This book was released on 2014 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the foundations of ontogeny by asking how the development of living things should be understood. It explores key concepts of developmental biology, asks whether general principles of development can be discovered, and what the role of models and theories is in developmental biology.
Download or read book Modern Theories of Development An Introduction to Theoretical Biology written by Ludwig Von Bertalanffy and published by . This book was released on 2014-11-01 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2014 Reprint of Original 1962 American Edition. Exact facsimile of the original edition, not reproduced with Optical Recognition Software. This important introduction to theoretical biology is a classic work in its field. Already in the 1930's Bertalanffy formulated the organismic system theory that later became the kernel of the GST (General Systems Theory]. GST is an interdisciplinary practice that describes systems with interacting components, applicable to biology, cybernetics, and other fields. Bertalanffy proposed that the classical laws of thermodynamics applied to closed systems, but not necessarily to "open systems," such as living things. His mathematical model of an organism's growth over time is still in use today.
Download or read book Birth and Fertility Rates for States written by Sally C. Clarke and published by Department of Health and Ters for Disease Company. This book was released on 1994 with total page 64 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gingival Diseases written by Fotinos Panagakos and published by BoD – Books on Demand. This book was released on 2011-09-22 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gingival diseases are a family of distinct pathological entities that involve the gingival tissues. These signs and symptoms of these diseases are so prevalent in populations around the world that they are often considered to be "normal" features. The diseases are now classified into two main groups namely: Plaque-Induced and Non-Plaque Induced Gingival Diseases. This book provides dentists, dental hygienists, dental therapists and students with a comprehensive review of gingival diseases, their aetiology and treatment.
Download or read book Early Mammalian Development written by Matthew H. Kaufman and published by CUP Archive. This book was released on 1983-10-06 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quinolone Antibacterials written by Jochen Kuhlmann and published by Springer. This book was released on 2012-02-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been over 30 years since the first clinically important member of the quinolone class, nalidixic acid, was introduced into medical practice. The modification produced in the quinolone nucleus by introducing a fluorine at the 6-position led to the discovery of the newer fluoroquinolones with enhanced antibacterial activities as compared to nalidixic acid. By now a great deal of preclinical and clinical experience has been obtained with these agents. The intense interest in this class of antibacterial agents by chemists, micro biologists, toxicologists, pharmacologists, clinical pharmacologists, and clini cians in various disciplines encouraged us to summarize the information on the history, chemistry, mode of action and in vitro properties, kinetics and efficacy in animals, mechanisms of resistance, toxicity, clinical pharmacology, clinical experience, and future prospects in one volume of the Handbook of Experimental Pharmacology. As this series deals predominantly with "experimental" characteristics of drugs, our volume is dedicated specifically to quinolones and emphasizes principally their preclinical and clinical phar macological characteristics, despite the existence of several summaries on quinolones. The chemistry of the quinolones is described in detail. The chapter on the mode of action of quinolones reports the conclusive evidence that gyrase is the intracellular target of the quinolones; however, another enzyme, topoisomerase IV, may also be a target for quinolones, and the exact mechanisms by which quinolones act bactericidally are far from being understood.
Download or read book Birth Defects and Drugs in Pregnancy written by Olli P. Heinonen and published by . This book was released on 1977 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt:
