Download or read book In Situ Hybridization Protocols written by Boye Nielsen and published by Humana Press. This book was released on 2014-09-13 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.
Download or read book In Situ Hybridization Methods written by Giselbert Hauptmann and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.
Download or read book Fluorescence In Situ Hybridization FISH Application Guide written by Thomas Liehr and published by Springer Science & Business Media. This book was released on 2008-11-26 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Download or read book FISH Technology written by Bernd W. Rautenstrauß and published by Elsevier. This book was released on 2002-02-14 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.
Download or read book Fluorescence In Situ Hybridization FISH for Microbial Cells written by Nuno F. Azevedo and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical in Situ Hybridization written by Trude Schwarzacher and published by Garland Science. This book was released on 2000 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical in situ Hybridization is aimed at those who wish to learn and use efficient and reliable protocols in their work, and at researchers checking the validity and interpretation of published data.; In situ hybridization methods can be used to identi
Download or read book In Situ Hybridization Protocols written by Ian A. Darby and published by Taylor & Francis US. This book was released on 2000 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Download or read book In Situ Hybridization in Electron Microscopy written by Gerard Morel and published by CRC Press. This book was released on 2001-01-30 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization
Download or read book Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences written by Julian E. Beesley and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.
Download or read book Tissue In Situ Hybridization written by Trevor Jowett and published by Wiley-Interscience. This book was released on 1997-02-05 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: TISSUE IN SITU HYBRIDIZATION Methods in Animal Development Trevor Jowett The European Molecular Biology Organization (EMBO) course on tissue in situ hybridization in animal developmental biology has served as an important and highly respected forum for the latest advances in the methodology of this valuable research tool. Developed from EMBO course materials, Tissue In Situ Hybridization provides scientists and researchers worldwide with detailed coverage of new approaches, techniques, and protocols, along with up-to-date information on more established procedures. Focusing particularly on the two-color in situ hybridization method to whole-mount embryos and tissue sections, this practical resource also compares different methods of producing differentially colored signals, and includes the results of protocol experiments with fluorescent and other alternative in situ hybridization techniques. Special features include: Photographic examples—including color plates—to complement the text Clear explanations of the principles underlying different methods Detailed discussion and comparison of the different methods Valuable troubleshooting advice and practical guidance Comprehensive index, allowing quick and easy access to specific topics Compiled by a leading expert in the field, Tissue In Situ Hybridization is an indispensable asset to professionals and researchers working in the areas of developmental, cell, and molecular biology.
Download or read book Modern Techniques in Cytopathology written by M.M. Bui and published by Karger Medical and Scientific Publishers. This book was released on 2020-01-13 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the era of precision medicine, physicians are increasingly in need of more definitive diagnostic, prognostic, and predictive information derived from small biopsy specimens such as cytology samples in order to guide effective patient care. Cytopathology is well poised to meet this challenge. Whilst the traditional cytomorphologic component of cytology practice is still valid, enormous advances have been made in the field of cytopathology thanks to transformative technology and innovative individuals that have augmented the cytologists' ability to meet the demands of modern medicine. The purpose of this book is to describe, illustrate, and review many of the most recent developments regarding modern techniques employed in cytopathology. This latest monograph is intended for all cytologists including cytopathologists, cytotechnologists, cytology lab assistants, trainees, research scientists, and anyone who is interested in the field of cytopathology. We have invited pioneering experts in their respective fields to author these chapters. This book is not only the culmination of their groundbreaking work and effort but also presents a critical review of the current literature. We have attempted to provide readers with an informative and comprehensive aid so that they may better appreciate how emerging technology has been applied to cytology. Each chapter in this book presents a stand-alone contemporary review of emerging topics in cytopathology. We hope that you will find this monograph thought-provoking and a valuable reference for your practice.
Download or read book ISCN 2013 written by International Standing Committee on Human Cytogenetic Nomenclature and published by Karger Medical and Scientific Publishers. This book was released on 2013 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication extends the now classic system of human cytogenetic nomenclature prepared by an expert committee and published in collaboration with Cytogenetic and Genome Research' since 1963. Revised and finalized by the ISCN Committee and its advisors at a meeting in Seattle, Wash., in April 2012, the ISCN 2013 updates, revises and incorporates all previous human cytogenetic nomenclature recommendations into one systematically organized publication that supersedes all previous ISCN recommendations. There are several new features in ISCN 2013: an update of the microarray nomenclature, many more illustrative examples of uses of nomenclature in all sections some definitions including chromothripsis and duplication a new chapter for nomenclature that can be used for any region-specific assay. The ISCN 2013 is an indispensable reference volume for human cytogeneticists, technicians and students for the interpretation and communication of human cytogenetic nomenclature.
Download or read book Medical BioMethods Handbook written by John M. Walker and published by Springer Protocols Handbooks. This book was released on 2005-03-18 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
Download or read book Introduction to Fluorescence In Situ Hybridization written by Michael Andreeff and published by Wiley-Liss. This book was released on 1999-12-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to Fluorescence in Situ Hybridization Principles and Clinical Applications Edited by Michael Andreeff, M.D., PH.D., and Daniel Pinkel, PH.D. Fluorescence In Situ Hybridization (FISH) has become an essential tool in the diagnosis and management of a variety of solid tumors and hematologic malignancies in the clinical setting, as well as an aid in the identification of particular genetic disorders. Introduction to Fluorescence In Situ Hybridization provides a solid groundwork in the basic principles and techniques of FISH and covers in detail the applications of this technology to cancer in humans, including tumorigenesis, prostate and breast tumors, myeloid leukemias, and lymphoproliferative malignancies. The final section of the book is devoted to the applications of FISH techniques to genetic analysis. This incorporates radiation biodosimetry in chromosomal aberrations, genetic toxicology, and multiprobe methods for detecting chromosomally defective sperm in humans and mice, plus rapid prenatal diagnosis of chromosomal aneuploidies and detection of fetal trisomy. Edited by two leading basic and clinical investigators in the field, with state-of-the-art contributions from expert subspecialists, and featuring over 200 color illustrations, Introduction to Fluorescence In Situ Hybridization is an essential reference for clinicians and investigators in oncology, genetics, and cytometry.
Download or read book Deep Sternal Wound Infections written by Raymund E. Horch and published by Springer. This book was released on 2017-01-19 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This concise and practical handbook covers the basics of pathophysiology, diagnosis, interdisciplinary surgical management, prevention and rehabilitation of patients with deep sternal wound infections and sternal osteomyelitis. All relevant aspects and surgical procedures are explained in an easily understandable way. Additionally special approaches and preventive measures are highlighted with regard to the perioperative handling as well as the rehabilitation possibilities. Through concise texts with numerous illustrations, the book is ideal for the practice and as a supplement to further studies. This book is suitable for all specialists who are involved into the treatment and diagnosis of sternal wound infections, particularly cardio-thoracis, thoracic, plastic, vascular surgeons, cardiologists, radiologists, and rehabilitation physicians.
Download or read book Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas written by M. A. Hayat and published by Academic Press. This book was released on 2006 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. The series provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book. Translates molecular genetics into cancer diagnosis and the results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations.
Download or read book Molecular Methods for Evolutionary Genetics written by Virginie Orgogozo and published by Humana Press. This book was released on 2011-03-15 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.
