Download or read book Improved Matrix assisted Laser Desorption ionization Linear Time of flight Mass Spectrometry of Oligonucleotides and Nucleic Acids Via Matrix Additvies and Sample Preparatory Technique written by Tracey Arlene Simmons and published by . This book was released on 2001 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2001 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fundamental Studies of Matrix assisted Laser Desorption ionization Time of flight Mass Spectrometry MALDI TOF MS and Its Applications to the Analysis of Biological Materials written by Yongdong Zhu and published by . This book was released on 1999 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Exploration of the Quantitative Aspects of Matrix assisted Laser Desorption Ionization Time of flight Mass Spectrometry written by Runbo Li and published by . This book was released on 2013 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advisor: Kevin G. Owens, Ph.D.
Download or read book Chemical Approaches to the Stabilization of Non covalent Complexes in Matrix assisted Laser Desorption ionization Mass Spectrometry written by Anne Marie Distler and published by . This book was released on 2003 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Matrix assisted Laser Desorption Ionization Time of flight Mass Spectrometry of Peptide Nucleic Acids for DNA Typing written by Ping Jiang-Baucom and published by . This book was released on 1997 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book MALDI MS written by Franz Hillenkamp and published by John Wiley & Sons. This book was released on 2013-12-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative book on MALDI MS, now finally available in its second edition and edited by one of its inventors, gives an in-depth description of the many different applications, along with a detailed discussion of the technology itself. Thoroughly updated and expanded, with contributions from key players in the field, this unique book provides a comprehensive overview of MALDI MS along with its possibilities and limitations. The initial chapters deal with the technology and the instrumental setup, followed by chapters on the use of MALDI MS in protein research (including proteomics), genomics, glycomics and lipidomics. The option of MALDI-MS for the analysis of polymers and small molecules are also covered in separate chapters, while new to this edition is a section devoted to the interplay of MALDI MS and bioinformatics. A much-needed practical and educational asset for individuals, academic institutions and companies in the field of bioanalytics.
Download or read book Evaluation of Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry for Clinical Microbiology Applications written by Elizabeth Bauerle and published by . This book was released on 2014 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: MALDI-TOF-MS has the ability to streamline identification of microorganisms in the microbiology lab. In this project, we focused on two main aims. First, the implementation of this technology for direct blood culture identification. Within this aim we determined the limit of detection for the protein purification method we planned on using in our laboratory, determined handling conditions for the samples, and evaluated the MALDI-TOF-MS for concordance with our current workflow and reports. Second, customization of the commercial library for Cryptococcus gattii was performed. Within this aim, we improved representation of the species in the MALDI-TOF-MS library, and also explored ways to determine the subspecies based on gathered spectra.
Download or read book Wire ion guide for improved performance in matrix assisted laser desorption ionization time of flight mass spectrometry written by Benjamin John Bolanos and published by . This book was released on 1995 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Two Laser Matrix Assisted Laser Desorption Laser Ionization Time of Flight Mass Spectrometry of Biomolecules written by and published by . This book was released on 1995 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent efforts to detect and identify large biomolecules using matrix assisted laser desorption have shown MALDI to be an extremely sensitive technique for trace detection from a solid sample. In the DI process, ions are formed by proton transfer from an electronically excited organic acid to a suitable proton accepting analyte. However, detection and characterization of biomolecules may benefit from ionization of the isolated unprotonated gas phase neutrals. To this end, a custom time-of-flight mass spectrometer as been constructed to perform two-laser matrix assisted laser desorption/laser ionization studies using separate desorption and ionization lasers. The two chamber, differentially pumped apparatus incorporates numerous laser and sample probe entry ports, a pulsed valve, and a modified Wiley-NcLaren mass spectrometer design. Results of initial two-laser experiments on phenylalanine in a sinnapinic acid matrix have demonstrated the apparatus' ability to detect laser ionization of laser desorbed neutrals.
Download or read book Mass Spectrometry of Proteins and Peptides written by John R. Chapman and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Little more than three years down the line and I am already writing the Preface to a second volume to follow Protein and Peptide Analysis by Mass . What has happened in between these times to make this second venture worthwhile? New types of mass spectrometric instrumentation have appeared so that new techniques have become possible and existing techniques have become much more feasible. More particularly, however, the newer ionization te- niques, introduced for the analysis of high molecular weight materials, have now been thoroughly used and studied. As a result, there has been an en- mous improvement in the associated sample handling technology so that these methods are now routinely applied to much smaller sample amounts as well as to more intractable samples. Again, this particular community of mass spectrometry users has both increased in number and diversified. And, riding this wave of acceptance, leaders in the field have set their sights on more complex problems: molecular interaction, ion structures, quantitation, and kinetics are just a few of the newer areas reported in Mass Spectrometry of Proteins and Peptides. As with the first volume, one purpose of this collection, Mass Spectr- etry of Proteins and Peptides, is to show the reader what can be done by the application of mass spectrometry, and perhaps even to encourage the reader to venture down new paths.
Download or read book Matrix assisted Laser Desorption ionization Mass Spectrometric Study of Biologic Molecules written by Yan-Hui Liu and published by . This book was released on 1996 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advances in MALDI and Laser Induced Soft Ionization Mass Spectrometry written by Rainer Cramer and published by Springer. This book was released on 2016-08-23 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics
Download or read book Matrix assisted Laser Desorption Ionization MALDI Time of flight Mass Spectrometry TOFMS for Direct Analysis of Bioactive Peptide and Protein written by and published by . This book was released on 1999 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall goal of this research was to develop a matrix assisted time-of-flight mass spectrometry (MALDI) method of mixture analysis based on time-lag focusing matrix-assisted MALDI (MALDI-TOFMS). The specific objectives were to develop sample/matrix preparation methods for mixture analysis and to demonstrate the overall analytical performance of the MALDI method for mixture analysis. This report describes and presents results of this research, including direct analysis of peptide and protein mixtures by MALDI-TOFMS. A two-layer sample preparation method is described as very effective for analyzing complex mixtures. In this method, the first layer on the MALDI probe is the densely packed matrix microcrystals formed by fast solvent evaporation of a matrix solution. A mixture solution containing both matrix and sample is then deposited onto the first layer to form uniform analyte/matrix micro-co-crystals. The effect of solvent conditions for preparing the second-layer solution is discussed.
Download or read book Nonpolar Matrices for Matrix Assisted Laser Desportion Ionization Time of Flight Mass Spectrometry written by and published by . This book was released on 2005 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been used extensively for the characterization of large biomolecules, synthetic polymers and small molecules. Typically, low molecular weight acidic matrices have been used in MALDI-MS for such analyses. Here, the use of low molecular weight nonpolar matrices for MALDI-MS is investigated. In particular, the analytical and physical properties of this class of matrices are studied. The effect of instrumental factors on nonpolar matrix behavior was investigated. Two different MALDI target substrates, stainless steel and poly(methylmethacrylate) (PMMA), were used. The radical molecular ion abundance of nonpolar analyte ions analyzed in the presence of various nonpolar matrices, such as anthracene and 9-cyanoanthracene, were determined as a function of the MALDI target substrate. It was found that PMMA MALDI target substrates yielded higher radical molecular ion abundances for nonpolar analyte ions than stainless steel MALDI target substrates. This difference is attributed to the absence of photoelectrons which reduce charged species in the plume. To determine whether the desorption process for nonpolar matrices is different than the desorption process for polar, acidic matrices, matrix initial ion velocities were measured in MALDI-MS. The relative initial ion velocities of the nonpolar matrices, anthracene, 9-cyanoanthracene, pyrene, and acenapthene and were investigated and compared to the relative initial ion velocities for 2,5-dihydroxybenzoic acid (DHB), sinapinic acid (SA), and all-trans retinoic acid (RTA), polar matrices. It was found that DHB has a greater relative initial ion velocity than the nonpolar matrices. It does not appear that the desorption process is different between nonpolar and polar matrices based upon these results. In addition, when using any matrix, polar or nonpolar, to analyze nonpolar polymers, the addition of a metal salt increased the relative initial ion velocity of the matrix, suggesting a different desorption mechanism in these cases. To illustrate an application of nonpolar matrices for analytical MALDI-MS, an atmospheric resid crude oil fraction was characterized. The MALDI mass spectral data for this sample was difficult to interpret when a polar matrix, 3-indole acrylic acid, was used, due to fragmentation and matrix clustering in the analyte molecular weight distribution range. The nonpolar matrices anthracene and 9-cyanoanthracene were found to be very suitable for the analysis of this sample in either linear or reflectron mode time-of-flight mass spectrometry. These matrices did not lead to fragmentation or clustering, and identification and characterization of the analyte peaks were straightforward.
Download or read book Matrix Assisted Laser Desorption ionization Studies in a Time of flight Mass Spectrometer written by Gabriela Grigorean and published by . This book was released on 1996 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Matrix assisted Laser Desorption Ionization Mass Spectrometry written by Jonathon Kevin Gooden and published by . This book was released on 2005 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:
