Download or read book High Performance Integrated Genetic Analyzers for Forensic DNA Typing written by Peng Liu and published by . This book was released on 2009 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfabrication technology offers great potential for the integration of all steps of forensic DNA typing onto a single microdevice. This integration should enable rapid, low-cost and reliable short tandem repeat (STR) analysis not only in forensic laboratories but also at crime scenes or other relevant point-of-analysis. As a first step towards making on-site STR typing possible, I developed a microdevice consisting of a 160-nL polymerase chain reaction (PCR) chamber and a 7-cm capillary electrophoresis (CE) channel and a portable instrument for its operation. A four-plex mini Y STR typing system was constructed for testing the capability of this microsystem for forensic STR typing. The successful analyses of casework and mixture samples validate the concept of forensic STR typing on a portable microfluidic system. To critically evaluate the capabilities of this portable system and the feasibility of DNA typing at a crime scene, real-time DNA analyses using a 9-plex autosomal STR typing system on a modified PCR-CE microdevice containing a co-injection structure for fragment sizing calculation were carried out at a mock crime scene. Blood stain collection, DNA extraction, STR analysis on the PCR-CE microsystem, and a DNA profile search against a mock CODIS database were successfully conducted within 6 hours of crime scene arrival. This demonstration establishes the feasibility of real-time DNA typing at a crime scene or other point-of-care situations. Finally, to achieve a total integrated analysis system for real-time STR typing, an up-front sequence-specific DNA extraction and concentration method using magnetic beads was developed and incorporated into the PCR-CE microdevice. Fragmented genomic DNA was hybridized with capture probes and immobilized onto magnetic beads via streptavidin-biotin binding in microchannels. The bead-DNA conjugates were then transported to a PCR reactor for amplification followed by inline injection using a novel capture concentration method for CE separation. This fully integrated system significantly advances the forensic DNA typing by providing a high-performance platform with sample-in-answer-out capability for real-time human identification.
Download or read book DNA Technology in Forensic Science written by National Research Council and published by National Academies Press. This book was released on 1992-02-01 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.
Download or read book Integrated Modular Microfluidic System for Forensic Alu Dna Typing written by Samuel Njoroge and published by LAP Lambert Academic Publishing. This book was released on 2012 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: To produce a microsystem with favorable performance characteristics for genetic-based analyses, several key operational elements must be strategically chosen, including device substrate material, temperature control, fluidic control and reaction product readout. The advantages afforded by fully integrated microfluidic systems to enable challenging applications, such as single-copy DNA sequencing, single-cell gene expression analysis, pathogen detection, and forensic DNA analysis in formats that provide high throughput and point-of-analysis capabilities must be addressed by the researcher. This book focus on discussion on microfluidic systems that are composed of two or more microdevices directed toward DNA analyses. The discussions primarily focus on the integration of various processing steps with u-capillary electrophoresis (uCE) with discoveries that have led to the development of fully autonomous and functional integrated systems for genome processing that can supply "sample in / answer out" capabilities."
Download or read book Advanced Topics in Forensic DNA Typing Interpretation written by John M. Butler and published by Academic Press. This book was released on 2014-07-28 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Topics in Forensic DNA Typing: Interpretation builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. Intended as a third-edition companion to the Fundamentals of Forensic DNA Typing volume published in 2010 and Advanced Topics in Forensic DNA Typing: Methodology published in 2012, this book contains 16 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field. Over 80 % of the content of this book is new compared to previous editions. - Provides forensic DNA analysts coverage of the crucial topic of DNA mixture interpretation and statistical analysis of DNA evidence - Worked mixture examples illustrate the impact of different statistical approaches for reporting results - Includes allele frequencies for 24 commonly used autosomal STR loci, the revised Quality Assurance Standards which went into effect September 2011
Download or read book DNA Technology in Forensic Science written by National Research Council and published by National Academies Press. This book was released on 1992-01-01 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.
Download or read book Forensic DNA Typing Principles Applications and Advancements written by Pankaj Shrivastava and published by Springer Nature. This book was released on 2020-12-11 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.
Download or read book Forensic DNA Typing Protocols written by Angel Carracedo and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.
Download or read book Forensic DNA Typing written by John M. Butler and published by Elsevier. This book was released on 2005-02-08 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic DNA Typing, Second Edition, is the only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome. It examines the science of current forensic DNA typing methods by focusing on the biology, technology, and genetic interpretation of short tandem repeat (STR) markers, which encompass the most common forensic DNA analysis methods used today. The book covers topics from introductory level right up to cutting edge research. High-profile cases are addressed throughout the text, near the sections dealing with the science or issues behind these cases. Ten new chapters have been added to accommodate the explosion of new information since the turn of the century. These additional chapters cover statistical genetic analysis of DNA data, an emerging field of interest to DNA research. Several chapters on statistical analysis of short tandem repeat (STR) typing data have been contributed by Dr. George Carmody, a well-respected professor in forensic genetics. Specific examples make the concepts of population genetics more understandable. This book will be of interest to researchers and practitioners in forensic DNA analysis, forensic scientists, population geneticists, military and private and public forensic laboratories (for identifying individuals through remains), and students of forensic science. *The only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome*Chapters cover the topic from introductory level right up to "cutting edge" research*High-profile cases are addressed throughout the book, near the sections dealing with the science or issues behind these cases*NEW TO THIS EDITION: D.N.A. Boxes--boxed "Data, Notes & Applications" sections throughout the book offer higher levels of detail on specific questions
Download or read book Advanced Topics in Forensic DNA Typing Methodology written by John M. Butler and published by Academic Press. This book was released on 2011-07-21 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt: John M. Butler.
Download or read book Handbook of DNA Forensic Applications and Interpretation written by Amit Kumar and published by Springer Nature. This book was released on 2022-07-01 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook covers tested and proven DNA forensic testing methodologies, forensic bioinformatics techniques, case studies and current forensic legal framework for investigation of variety of crimes and provides a clinching evidence for speedy justice. DNA testing is widely used for forensic purposes and is changing the paradigm of (crime) investigation. The book contains chapters on usage of ultramodern DNA collection kits, presents era evidence collection and preservation, high-end DNA sample analysis in laboratory, DNA legislation, expert evidences, challenging and successful case studies, data generation and application of AI and IoT techniques for DNA data analysis, DNA databanks and training manpower to facilitate timely reporting to the requesting agencies. This handbook equips and enables police, investigators and crime analysis laboratories with knowhow of high-end tools, procedures and techniques to link or exclude a criminal to a crime. It is expected that this will be used by first responders, police, forensic analysts, judiciaries, evidence handlers and students and scholars of criminology and forensic sciences worldwide. The intention to write this handbook is to make DNA technology and its importance reach every common man and professional for correctly using it as a tool as and when required. This is quite evident that awareness of DNA technology has increased at a reasonable pace. Courts and investigating agencies are convinced and confident with its accuracy, reliability and unmatched peace delivered by various techniques of DNA fingerprinting and DNA profiling.
Download or read book Forensic DNA Analysis written by Elena Pilli and published by CRC Press. This book was released on 2021-03-31 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic DNA Analysis: Technological Development and Innovative Applications provides a fascinating overview of new and innovative technologies and current applications in forensic genetics. Edited by two forensic experts with many years of forensic crime experience with the Italian police and with prestigious academic universities, the volume takes an interdisciplinary perspective, the volume presents an introduction to genome polymorphisms, discusses, forensic genetic markers, presents a variety of new methods and techniques in forensic genetics, and looks at a selection of new technological innovations and inventions now available from commercial vendors. The book is an important resource for scientists, researchers, and other experts in the field who will find it of interest for its exhaustive discussion of the most important technological innovations in forensic genetics. For those newer to the field, the volume will be an invaluable reference guide to the forensic world.
Download or read book Fundamentals of Forensic DNA Typing written by John M. Butler and published by Academic Press. This book was released on 2009-09-30 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Forensic DNA Typing is written with a broad viewpoint. It examines the methods of current forensic DNA typing, focusing on short tandem repeats (STRs). It encompasses current forensic DNA analysis methods, as well as biology, technology and genetic interpretation. This book reviews the methods of forensic DNA testing used in the first two decades since early 1980's, and it offers perspectives on future trends in this field, including new genetic markers and new technologies. Furthermore, it explains the process of DNA testing from collection of samples through DNA extraction, DNA quantitation, DNA amplification, and statistical interpretation. The book also discusses DNA databases, which play an important role in law enforcement investigations. In addition, there is a discussion about ethical concerns in retaining DNA profiles and the issues involved when people use a database to search for close relatives. Students of forensic DNA analysis, forensic scientists, and members of the law enforcement and legal professions who want to know more about STR typing will find this book invaluable. - Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect - Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes-- "Data, Notes & Applications" sections throughout - Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key
Download or read book Advancing Forensic DNA Profiling Through Microchip Technology written by Hang Ieng Yeung and published by . This book was released on 2008 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Future of Forensic DNA Testing written by and published by . This book was released on 2000 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: "A report from National Commission on the Future of DNA Evidence"--Cover.
Download or read book Forensic DNA Typing written by John Marshall Butler and published by . This book was released on 2005 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of DNA Profiling written by Hirak Ranjan Dash and published by Springer. This book was released on 2022-06-29 with total page 1206 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book comprehensively reviews the significance of DNA technology in forensic science. After presenting the theory, basic principles, tools and techniques that are used in forensic DNA typing, it summarizes various techniques, including autosomal STR, Y-STR, X-STR, mitochondrial DNA and NGS, used in solving both criminal as and civil cases, such as paternity disputes, identification of mutilated remains, and culprit identification in sexual assault and murder cases. It also provides an overview of DNA-based genetic diagnostics for various diseases, and discusses the role of DNA typing in drug reactions, as well as the application of non-human DNA profiling of animals and plants in forensic science investigations. Lastly, the book examines the role of internal quality control in maintaining the high quality of DNA profiling.
Download or read book Genetic Testimony written by Charlotte Spencer and published by Prentice Hall. This book was released on 2004 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: For undergraduate courses in introductory-level Human Genetics, Biochemistry, and Molecular Biology courses. Also appropriate as a resource for law schools, legal clinics, and law enforcement offices. Part of the "Prentice Hall Exploring Biology Series", DNA Forensics explores the subject of modern DNA profiling in straightforward language, requiring and is aimed at students with little background in science or biotechnology. It raises controversial questions about the uses and potential misuses of DNA forensics; and illustrates issues by presenting recent criminal cases involving DNA profiling. A valuable resource for undergraduate science students, it introduces basic concepts of genetics and biotechnology in the context of one of the most important developments in modern criminal investigation.
