Download or read book Handbook of Affinity Chromatography written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
Download or read book Handbook of Affinity Chromatography Second Edition written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatography reflects upon important factors to consider in the development of affinity methods, such as the choice of support material, immobilization methods, and application or elution conditions. It reviews common affinity methods and explores the latest preparative, analytical, and biophysical applications, including the use of affinity chromatography with other separation techniques and analytical systems. This basis seamlessly supports the discussion of recent developments in techniques including the use of affinity ligands in capillary electrophoresis, mass spectrometry, microanalytical systems, and optical biosensors. New chapters feature expanded discussions on molecularly imprinted polymers and biomimetic ligands, chromatographic immunoassays, affinity-based immunoassays, affinity-based chiral stationary phases, and affinity ligands in multidimensional systems. Engaging the collaboration of 48 scientists and students from 23 laboratories and organizations to present the latest information on affinity methods, the Handbook of Affinity Chromatography illustrates a wide range of applications and theory for scientists, students, and laboratory workers throughout the fields of chemistry and biology.
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Download or read book Affinity Chromatography written by Michael Zachariou and published by Springer Science & Business Media. This book was released on 2008 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.
Download or read book Handbook of HPLC written by Danilo Corradini and published by CRC Press. This book was released on 2016-04-19 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the
Download or read book Guide to Protein Purification written by Richard R Burgess and published by Academic Press. This book was released on 2009-11-03 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Download or read book Liquid Chromatography written by D.S. Hage and published by Elsevier Inc. Chapters. This book was released on 2013-01-08 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography is a type of liquid chromatography that uses a biologically related agent as a stationary phase to purify or analyze specific sample components. This chapter examines the basic components and principles of affinity chromatography and various applications of this method. The general types of ligands, immobilization schemes, support materials, and elution conditions that can be employed in affinity chromatography are first examined. Various methods in this field are then considered, including bioaffinity chromatography, immunoaffinity chromatography, dye-ligand and biomimetic affinity chromatography, immobilized metal-ion affinity chromatography, and analytical affinity chromatography. The types of ligands utilized in each of these methods are described, along with common applications of these techniques. Several related developments are also discussed, such as the use of affinity chromatography with mass spectrometry, miniaturized systems, molecularly imprinted polymers, and aptamers.
Download or read book Affinity Chromatography written by and published by . This book was released on 2002 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Process Chromatography written by Gail K. Sofer and published by Academic Press. This book was released on 1997-06-24 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: With examples from companies with establised processes and approved biotherapeutics, this pack considers the entire scope of process chromatography, including scale-up, regulatory issues, equipment, evaluation studies, scheduling and cost effectiveness.
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Download or read book Handbook on Protein Purification written by Nikolaos E. Labrou and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are important biomolecules that are vital for the cellular structure and function. They perform a vast array of functions within organisms, including the catalysis of metabolic reactions, DNA replication, response to stimuli, and transporting molecules from one location to another. The technological advances in the omics areas (e.g., genomics, transcriptomics, proteomics, metagenomics, etc.) have dramatically increased the rate of discovering new proteins. Some of them hold large opportunities for innovative research and the development of commercial products and applications. It is worth noting that the global protein ingredients market is poised to grow over the next decade to reach approximately 58.49 billion USD by 2022, with the protein therapeutics market valuing around USD 315.9 billion by 2025. Interestingly, about seventy monoclonal antibody products will be on the market by 2020 with a combined worldwide sales of about 125 billion USD.The most significant parameter for the successful commercial exploitation of proteins rely on the development of an efficient and effective isolation and purification technology, known as protein downstream processing. Downstream processing refers to the technology that involves the isolation and production of purified products from natural sources such as animal tissues, plant tissues, microorganisms or fermentation broth. The most important element of this technology is the high purification processes, most important of which is chromatography and in particular affinity chromatography.This book provides information on the resent developments of protein downstream processing and deals with the information gained over the last years from the application of protein purification technologies on different research areas. Each chapter gives key examples that cover a wide range of diverse scientific disciplines in order to provide the reader with a representative sample of the current status of the field. The present book would definitely be an ideal source of scientific information to the advanced students, junior researchers, and scientists involved in cellular and molecular biology, biochemistry, microbiology, biotechnology and other related areas.
Download or read book HPLC Made to Measure written by Stavros Kromidas and published by John Wiley & Sons. This book was released on 2008-06-05 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.
Download or read book Affinity Chromatography written by J. Turková and published by Elsevier. This book was released on 1978-01-01 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.
Download or read book A Practical Handbook of Preparative HPLC written by Donald A Wellings and published by Elsevier. This book was released on 2011-04-18 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.This book...•Provides practical, hands-on advice based on years of experience•Will help ensure optimal design, equipment and separation results for your particular task•Presents system layouts from laboratory to process scale•Will help you to devise or improve record-keeping and documentation systems ·Provides practical, hands-on advice based on years of experience·Will help ensure optimal design, equipment and separation results for your particular task·Presents system layouts from laboratory to process scale·Will help you to devise or improve record-keeping and documentation systems
Download or read book Handbook of Process Chromatography written by Gunter Jagschies and published by Elsevier. This book was released on 2007-12-08 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will update the original edition published in 1997. Since the publication of the first edition, the biotechnology and biologics industries have gained extensive knowledge and experience in downstream processing using chromatography and other technologies associated with recovery and purification unit operations. This book will tie that experience together for the next generation of readers. Updates include: - sources and productivity - types of products made today - experiences in clinical and licensed products - economics - current status of validation - illustrations and tables - automated column packing - automated systems New topics include: - the use of disposables - multiproduct versus dedicated production - design principles for chromatography media and filters - ultrafiltration principles and optimization - risk assessments - characterization studies - design space - platform technologies - process analytical technologies (PATs) - biogenerics - comparability assessments Key Features: - new approaches to process optimiaztion - use of patform technologies - applying risk assessment to process design
Download or read book Affinity Chromatography written by Sameh Magdeldin and published by BoD – Books on Demand. This book was released on 2012-03-21 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most will agree that one major achievement in the bio-separation techniques is affinity chromatography. This coined terminology covers a myriad of separation approaches that relies mainly on reversible adsorption of biomolecules through biospecific interactions on the ligand. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exemplarily applications of this versatile technique. We have always been endeavor to keep the contents of the book crisp and easily comprehensive, hoping that this book will receive an overwhelming interest, deliver benefits and valuable information to the readers.
Download or read book Handbook of HPLC written by Danilo Corradini and published by CRC Press. This book was released on 2011-01-03 with total page 1018 pages. Available in PDF, EPUB and Kindle. Book excerpt: Delineating its usage in separation, purification and detection processes across a variety of disciplines, from industry to applied research, this work discusses the principles, techniques and instrumentation involving HPLC within a detailed framework. Over 100 tables present previously scattered experimental data.
