Download or read book General Cytochemical Methods written by James Frederic Danielli and published by . This book was released on 1961 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book General Cytochemical Methods written by J.F. Danielli and published by Elsevier. This book was released on 2012-12-02 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: General Cytochemical Methods, Volume II focuses on methods and techniques employed in the studies of the biochemistry of cells. Composed of eight chapters, the book looks at immersion refractometry of living cells by phase contrast and interference microscopy. Areas considered include interpretation of refractive index measurements as indicator of hydration; immersion refractometry with phase-contrast microscopy; and practical aspects of checking phase-change with interference microscopes. The text continues with the discussions on the Cartesian diver balance method. Particularly noted are the standards, principle, applications, and precision of the method, which has been proven effective in microgasometric measurements. The book also focuses on quantitative determination through a special ""ampulla-diver"" of the cholinesterase activity in cells. Given attention are the materials, methods, and results of experiments. The text also looks at periodate oxidation techniques; acylation and diazonium coupling in protein cytochemistry with special reference to the benzoylation-tetrazonium method; and the use of dinitrobenzene as a cytochemical reagent. The book is a great find for readers interested in studying the biochemistry of cells.
Download or read book General Cytochemical Methods written by James Frederic Danielli and published by . This book was released on 1961 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Stains and Cytochemical Methods written by M.A. Hayat and published by Springer Science & Business Media. This book was released on 1993-03-31 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: The author's primary aim is to thoroughly explain the biochemical concepts governing cytochemical procedures for transmission electron microscopy. Such information provides undergraduate and graduate students, technicians, and researchers with a more profound understanding of electron micrographs, as well as the knowledge to refine existing techniques and develop new methodologies.
Download or read book General Cytochemical Methods V 1 written by James Frederic Danielli and published by . This book was released on 1958 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book General Cytochemical Methods written by and published by . This book was released on 1958 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Histochemical and Cytochemical Methods of Visualization written by Jean-Marie Exbrayat and published by CRC Press. This book was released on 2013-06-05 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio
Download or read book Cytochemical Staining Methods for Electron Microscopy written by Peter Raymond Lewis and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book General Cytochemical Methods written by James Frederic Danielli and published by . This book was released on 1958 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cytochemical Bioassays written by J Chayen and published by Butterworth-Heinemann. This book was released on 2013-10-22 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytochemical Bioassays: Techniques and Clinical Applications describes the techniques and clinical applications of cytochemical bioassays, particularly with respect to polypeptide hormones. This book discusses the clinical and research implications of studies that use these techniques. This text is comprised of 14 chapters; the first of which introduces the reader to the origins and general principles of cytochemical bioassays, along with their advantages. This discussion is followed by a chapter that explains what is meant by ""an assay""; for what purpose assays are done; the basic components of an assay method; and the nature of the difference between in vivo and in vitro hormone bioassays, in vitro ligand assays, and enzyme assays. Attention then turns to the techniques of cytochemical bioassays and the cytochemical bioassay procedures for adrenocorticotropic hormones; thyroid-stimulating hormones; the thyroid-stimulating antibody of Graves' disease; thyroid growth stimulating and blocking immunoglobulins; and luteinizing hormones. The next chapters focus on the technique and clinical relevance of a cytochemical bioassay for gastrin-like activity and for measurement of antidiuretic hormones, parathyroid hormones, angiotensin II, natriuretic hormones, and hypothalamic regulating hormones. This book will be of interest to clinicians and practitioners of biology, biochemistry, and endocrinology.
Download or read book Immunocytochemical Methods and Protocols written by Constance Oliver and published by Humana Press. This book was released on 2012-04-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.
Download or read book Cytochemistry written by James Frederic Danielli and published by . This book was released on 1953 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fixation procedures; Studies on alkaline phosphatase; The critical study of the cytochemistry of aldehydes; The cytochemistry of proteins and nucleic acids; Quantitative studies in cytochemistry; The future outlook in cytochemistry.
Download or read book Cytochemical Methods with Quantitative Aims written by International Society for Cell Biology and published by . This book was released on 1957 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Histochemistry of Single Molecules written by Carlo Pellicciari and published by Springer Nature. This book was released on 2022-09-24 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.
Download or read book Electron Microscopy of Plant Cells written by C Hawes and published by Academic Press. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Download or read book The Leukemia Lymphoma Cell Line Factsbook written by Hans G. Drexler and published by Academic Press. This book was released on 2000-09-04 with total page 751 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Leukemia-Lymphoma Cell Line Factsbook represents an essential reference manual for all of the well-characterized leukemia-lymphoma cell lines currently available. It provides the most important facts, using the succinct and user-friendly format that has made the FactsBooks so popular with scientists and clinical researchers. Introductory chapters provide background and perspective for culturing malignant hematopoietic (blood forming) cell lines. These chapters are followed by over 400 comprehensive individual entries. Each cell line entry highlights essential clinical, immunological, genetic, and functional features and includes a comprehensive listing of references. - The full spectrum of malignant cell lines from all hematopoietic cell lineages - Sister cell lines and relevant subclones - Clinical data: patient, diagnosis, treatment status, and specimen source - Authentication of derivation and availability - Immunophenotype - Cytogenetic karyotype - Translocations and fusion genes - Receptor gene rearrangements and genetic alterations - Cell cultures aspects: establishment, medium, doubling time, growth - Cytochemical profile - Cytokine production and response to cytokines - Proto-oncogene and transcription factor expression/alteration - Functional features: differentiation induction, heterotransplantability - Special unique features - Key references
