Download or read book Further Characterization of the UL37 Protein of Herpes Simplex Virus Type 1 and Its Interaction with ICP8 the Major DNA binding Protein of Herpes Simplex Virus written by Allen G. Albright and published by . This book was released on 1994 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of the UL37 Protein of Herpes Simplex Virus Type 1 and Demonstration that it Interacts with ICP8 the Major DNA Binding Protein of Herpes Simplex Virus written by Lisa S. G. Shelton and published by . This book was released on 1992 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Download or read book Characterization of the Herpes Simplex Virus 1 DNA Polymerase and Its Interaction with UL42 Protein and ICP8 and Mutational Analysis of a Viral Origin of Replication written by Thomas R. Hernandez and published by . This book was released on 1993 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Herpes Simplex Virus 1 DNA Packaging written by Ying Fan and published by . This book was released on 1997 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Significance of the Physical Interaction Between the Herpes Simplex Virus Type 1 Origin binding Protein UL9 and the DNA Polymerase Processivity Factor UL42 written by Kelly S. Trego and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The origin (ori)-binding protein of herpes simplex virus type 1 (HSV-1), encoded by the UL9 open-reading frame, has been shown to physically interact with a number of cellular and viral proteins, including three HSV-1 proteins (ICP8, UL42, and UL8) essential for ori-dependent DNA replication. In this report, it is demonstrated for the first time that the DNA polymerase processivity factor, UL42 protein, provides accessory function to the UL9 protein, by enhancing the 3' to 5' helicase activity of UL9 on partially duplex non-specific DNA substrates. UL42 fails to enhance the unwinding activity of a non-cognate helicase, suggesting enhancement of unwinding requires the physical interaction between UL42 and UL9. UL42 increases the steady-state rate for unwinding a 23/38-mer by UL9, but only at limiting UL9 concentrations, consistent with a role in increasing the affinity of UL9 for DNA. Optimum enhancement of unwinding was observed at UL42:UL9 molecular ratios of 4:1, although enhancement was reduced when high ratios of UL42:DNA were present. Under the assay conditions employed, UL42 did not alter the rate of dissociation of UL9 from the DNA substrate. UL42 also did not alter the requirement or time for an assembly/conformational change step, regardless of whether it was added to DNA prior to or at the same time as UL9, or after steady-state unwinding by UL9 alone had been achieved. Thus, the increased affinity of UL9 for DNA most likely is the result of an increase in the association rate constant for binding of UL9 to DNA, and explains why helicase enhancement is observed only at subsaturating concentrations of UL9 with respect to DNA. Consistent with this interpretation are results which demonstrate that UL42 also enhances the ATPase activity of UL9 on single-strand and partially duplex DNA substrates when UL9 is limiting. In contrast, ICP8 enhances unwinding at both saturating and subsaturating UL9 concentrations, and reduces or eliminates the lag period. The different means by which ICP8 and UL42 enhance activities of UL9 suggest that these two members of the presumed functional replisome may act synergistically on UL9 to effect initiation of HSV-1 DNA replication in vivo.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1995 with total page 666 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Further Characterization of the Herpes Simplex Virus Type 1 Thymidine Kinase Pre mRNA Processing Enhancer written by Dana M. Willard and published by . This book was released on 1998 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structure function Analysis of the Herpes Simplex Virus Type 1 Origin Binding Protein UL9 written by Ajay Kumar Malik and published by . This book was released on 1996 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Expression of the Herpes Simplex Virus Type 1 ICP22 and Us1 5 Proteins written by John Jason Bowman and published by . This book was released on 2008 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using this system and newly generated C-terminal specific ICP22 Abs, we show that Us1.5 expression is independent of full length ICP22. In addition, we demonstrate that the reported Us1.5 start sites, M147 and M171 are incorrect and that Us1.5 initiates translation at M90 of the ICP22 ORF. Mutation of M90 to alanine resulted in loss of Us1.5 expression but had no effect on expression of the full length protein. We introduced the M90A mutation into the viral genome and, as observed in transfected cells, Us1.5 expression was greatly reduced in M90A-infected cells. Although Us1.5 expression was greatly reduced, the M90A mutant replicated efficiently in restrictive Rab-9 cells and levels of the late gene gC accumulated to WT levels. Collectively, this dissertation describes multiple systems to transiently express ICP22. Using this system we mapped the true start site of the Us1.5 protein and raised questions to the origin of Us1.5 protein expression.
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1994 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of the Herpes Simplex Virus Type 1 Virion Host Shutoff Protein written by Bradley M. Karr and published by . This book was released on 1994 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Interactions Between the Tegument Proteins UL11 and UL16 and the Glycoprotein E of Herpes Simplex Virus written by Pei-Chun Yeh and published by . This book was released on 2009 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: The herpesvirus tegument region present between the virion envelope and the nucleocapsid contains more than 20 different virus-encoded proteins. The process of tegument assembly and final envelopment has been unfoldeding over the past few decades. It is thought that a few tegument proteins are added to the capsid in the nucleus, whereas most of them are acquired after entering the cytoplasm or traveling to the site of final envelopment at the trans-Golgi network. Overall, the research presented in this dissertation provides insights into the molecular mechanism of protein-protein interactions that may be involved in (or contribute to) assembly and maturation of herpes simplex virus type 1 (HSV-1). The UL11 (membrane-bound) and UL16 (capsid-associated) tegument proteins are conserved among all herpesviruses, and interaction between the two has been implicated in linking the viral capsid, tegument, and membrane during final envelopment process. Both in transfected and virus-infected cells, a subpopulation of the UL11 protein was found associated with detergent-resistant membranes via modifications with two fatty- acid chains (myristate and palmitate). UL11 can directly interact with UL16 in a manner dependent on the acidic cluster and leucine-isoleucine motifs of UL11. And, N-ethylmaleimide-modified UL16 was found to be incapable of binding UL11, suggesting that free cysteines in UL16 somehow play a role in the interaction. UL16 is stably associated with cytoplasmic capsids isolated from infected cells. In response to initial attachment of virus to the cell surface, an 'outside-in' signal is transmitted across the virion membrane, and as a result, UL16 is dissociated from the capsid. The mechanism by which the signal is sent to UL16 remains unclear but seems likely to be mediated by the glycoproteins on the virion envelope. A GST chimera bearing the cytoplasmic tail of glycoprotein E (gE. CT) was found to be capable of binding UL16 expressed in mammalian or insect cells. To better understand the molecular mechanism of this signaling process, the interaction network emanating from UL16 was investigated. In addition, previously using a GST pull-down assay, it was observed that UL16 interacts with virus-specific proteins from HSV- and PRV-infected cell lysates, providing evidence that UL16 is present in protein complexes. To characterize native complexes that contain UL16, a recombinant virus was constructed expressing a tagged derivative. Using the combination of tandem affinity purification and mass spectrometry analysis, we identified gE to be present in the complexes isolated from infected cells. The UL16-gE interaction was confirmed in co-immunoprecipitation assays with infected cell lysates. Moreover, mutational analyses of gE. CT have suggested that in infected cells UL16 may interact with gE. CT in both UL11-dependent and -independent manners. Based on all available data, we hypothesize that UL11, UL16, and gE may form a tripartite complex which plays a role in multiple aspects of the virus life cycle, including signaling events during virus attachment, virion maturation, or cell-to-cell spread. Collectively, our research focused on the protein-protein network has built a foundation for future studies, and also advanced our current knowledge of herpesvirus replication.
Download or read book HSV 1 Exonuclease UL12 Interacts with Host Recombination Factors and is Essential for Viral Growth written by Nandakumar Balasubramanian and published by . This book was released on 2011 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of Host Proteins Involved in Herpes Simplex Virus Type 1 DNA Synthesis written by Whitney Cogswell and published by . This book was released on 2008 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Herpes simplex virus type 1 (HSV-1) is the prototypic member of a large family of viruses known as Herpesviridae. Herpesviruses possess a double-stranded DNA genome and infect a broad range of species. In addition to HSV-1, there are seven other herpesviruses that are known to infect humans. Although antiviral treatment is available for HSV infections, there are few or no options for treatment of infections by other herpesviruses. One of the best targets for development of new antivirals is the viral DNA replication apparatus. HSV-1 encodes for seven proteins directly involved in viral DNA replication. However, no HSV-1 origin-dependent DNA replication has been reconstituted in vitro using purified viral proteins. It is hypothesized that proteins (host and/or viral) in addition to the seven known viral DNA replication proteins are involved in viral DNA replication in vivo. Moreover, it is predicted that such proteins would interact with one or more of the viral DNA replication proteins. In an effort to identify interacting host proteins, co-immunoprecipitation analysis was performed using antibodies specific for an essential HSV DNA replication protein, UL42, the polymerase processivity factor. Extracts from infected and mock-infected cells were immunoprecipitated with the UL42 antibody. UL42, and all that is bound to it, bind to the antibody which binds to the Protein G agarose matrix. Those proteins that are specifically pulled down from infected cell extracts, but not from mock-infected extracts, were to be identified by mass-spectrometry. The immunoprecipitates were analyzed following separation on denaturing polyacrylamide gels. Gels were silver-stained or analyzed for the presence of UL42 using a Western blot. Conditions were adjusted to optimize the amount of UL42 immunoprecipitated, but UL42 could not be immunoprecipitated quantitatively. Following immunoprecipitation under optimum conditions, no differences were observed between the proteins pulled-down from mock- or infected-cell extracts. Due to the poor ability of the antibody to pull-down UL42, a second approach was employed. A histidine-tagged version of UL42 was purified using a nickel chelating column and then mixed with infected or mock-infected cell extracts. Those proteins that specifically interact with his-UL42 were expected to bind to a nickel column. However, nonspecific interactions with the matrix were observed and prevented identification of proteins by mass-spectrometry.
Download or read book Recombinant Virus Vaccines written by Maureen C. Ferran and published by Humana. This book was released on 2018-06-09 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms. Recombinant Virus Vaccines: Methods and Protocols details the use of recombinant vaccines that are employed to either produce immunogens in vitro or elicit antibody production in vivo. The chapters in this book are divided into four parts: Part I explores double-stranded DNA viruses; Part II discusses negative sense single-stranded RNA viruses; Part III talks about positive sense single-stranded RNA viruses; and Part IV describes bacteriophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Recombinant Virus Vaccines: Methods and Protocols is a valuable resource for scientists and clinicians who are interested in learning about and adopting methods for use in basic and biomedical research directed toward generating and developing recombinant viral vaccines.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1991 with total page 1488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
