Download or read book Fluorescence In Situ Hybridization FISH for Microbial Cells written by Nuno F. Azevedo and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence In Situ Hybridization FISH for Microbial Cells written by Nuno F. Azevedo and published by Humana. This book was released on 2021-03-09 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.
Download or read book Fluorescence In Situ Hybridization FISH Application Guide written by Thomas Liehr and published by Springer Science & Business Media. This book was released on 2008-11-26 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Download or read book Fluorescence In Situ Hybridization FISH written by Gal Haimovich and published by Springer Nature. This book was released on with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacteria in Their Natural Environments written by Madilyn Fletcher and published by Academic Press. This book was released on 1985 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book FISH Technology written by Bernd W. Rautenstrauß and published by Elsevier. This book was released on 2002-02-14 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.
Download or read book Chromosome Structure and Aberrations written by Tariq Ahmad Bhat and published by Springer. This book was released on 2017-02-08 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compilation of various chapters contributed by a group of leading researchers from different countries and covering up to date information based on published reports and personal experience of authors in the field of cytogenetics. Beginning with the introduction of chromosome, the subsequent chapters on organization of genetic material, karyotype evolution, structural and numerical variations in chromosomes, B-chromosomes and chromosomal aberrations provide an in-depth knowledge and easy understanding of the subject matter. A special feature of the book is the inclusion of a series of chapters on various types of chromosomal aberrations and their impact on breeding behaviour and crop improvement. The possible mechanism, their consequences and role in genetic analysis has been emphasized in these chapters. A few chapters have also been dedicated on various techniques routinely used in the laboratory by students and researchers. Each chapter ends with an extensive bibliography so that the students and researchers may find it relevant to consult more literature on the subject than a book of this size can offer. The book is intended to fulfill the needs of undergraduate and post graduate students of botany, zoology and agriculture besides, teachers and researchers engaged in the field of genetics, cytogenetics, and molecular genetics. In general the readers will find each chapter of the book informative and easy to understand.
Download or read book Fluorescence in situ Hybridization FISH written by Joanna M. Bridger and published by Humana. This book was released on 2016-08-23 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.
Download or read book Fluorescence In Situ Hybridization FISH Application Guide written by Thomas Liehr and published by Springer. This book was released on 2008-11-18 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Download or read book Using Fluorescence in Situ Hybridization to Study Intracellular Properties written by Jan David Brüwer and published by . This book was released on 2024 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microorganisms dominate the surface ocean and significantly shape biogeochemical cycles, particularly the carbon cycle. Through photosynthesis, primary producers fix inorganic carbon and produce organic matter. The phytoplankton-derived organic matter fuels differently specialized heterotrophic bacteria that remineralize large proportions of it. At the same time, phages, which are viruses that infect bacteria, shape the bacterial community composition through top-down controls. Environmental microbial ecology often uses microscopically derived changes in cell abundance or incubation experiments with labeled substrates to derive bacterial growth or activity. However, the former does not account for mortality, while the latter is prone to biases by bottle effects and substrate preferences. Nevertheless, marine microbial ecology requires a thorough understanding of bacterial growth and mortality to understand the effects on, e.g., the carbon cycle. Additionally, microbial ecologists have little understanding of the effect of phages on the heterotrophic community. For example, no studies have quantified the amount of phage-infected heterotrophs in complex marine samples to date. For this thesis, I studied the bacterial life cycle, from cell division to cell death, in environmental samples. To do so, I used fluorescence in situ hybridization (FISH) techniques and high-throughput image cytometry, complemented with metagenomic analyses. A significant focus was on bacteria of the SAR11 clade. SAR11 are specialized to grow in oligotrophic habitats that are nutrient and substrate-depleted. They are assumed to be outcompeted by specialized bacteria during high-substrate conditions, such as phytoplankton blooms. Additionally, there is an ongoing debate on whether phage infection has a considerable effect on SAR11 communities. In this thesis, I demonstrate rapidly dividing SAR11 communities during phytoplankton blooms with a concomitantly high phage infection rate. In Chapter 2, I used FISH in combination with a fluorescent DNA stain to study the frequency of dividing cells by visualizing the intracellular DNA distribution. During a cell replication cycle, the dublicated genomes need to be separated into the future daughter cells. The correlation with experimentally derived in situ cell division rates allowed the calculation of cell division across an entire phytoplankton bloom. Measurements revealed faster SAR11 cell division rates than anticipated by cell counts. Hence, calculated mortality rates were high during these times. As the mortality was taxon-specific, I hypothesized phage-induced lysis as a cause. In Chapter 3, I designed direct-geneFISH probes to visualize phage-infected SAR11 cells in the environment. I could thereby provide the missing link between cell division and mortality rates, as revealed in Chapter 2. The highest amounts of phage-infected SAR11 cells (up to 19 % of the SAR11 cells) were detected when taxon-specific mortality and cell division rates were highest. Additionally, I found a phenomenon of phage-infected, ribosome-depleted cells, which I dubbed 'zombie cells'. I thoroughly discuss possible explanations for their emergence and propose that nucleotides from ribosomal RNA are used as substrates for phage genome synthesis. Additionally, I show that both phage-infected and zombie cells occur globally. In Chapter 4, I assessed the influences of future ocean scenarios and a marine heatwave on the microbial community, including cell division and grazing rates. Anthropogenic influences shape the ocean as a habitat with unknown consequences for the microbial community. I found no significant influences of the mild marine heatwave, while the future ocean scenarios caused differences in bacterial abundances. Overall, the results indicate a stable and adaptable marine microbial community in the face of a changing ocean. In the general discussion (Chapter 5), I discuss the methodological approaches used in this thesis to study bacterial cell division rate and the viral community. I further summarize and discuss the insights into SAR11 ecology gained through this thesis, as well as the importance and emergence of zombie cells. The discussion is rounded off with an outlook, proposing directions for future research projects to understand further the interplay of bacterial hosts and their phages, as well as zombie cells.
Download or read book FISH Technology written by Bernd W. Rautenstrauß and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.
Download or read book Peptide Nucleic Acids written by Peter E. Nielsen and published by Humana Press. This book was released on 2002-07-23 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA—an uncharged pseudopeptide—that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA—many of which have not yet been validated— I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .
Download or read book The Quest for a Universal Theory of Life written by Carol E. Cleland and published by Cambridge University Press. This book was released on 2019-09-12 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores fundamental philosophical and scientific questions about the nature of life, particularly in relation to the search for extraterrestrial life.
Download or read book Open Space Microfluidics written by Emmanuel Delamarche and published by John Wiley & Sons. This book was released on 2018-01-18 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.
Download or read book Veterinary Infection Biology Molecular Diagnostics and High Throughput Strategies written by Mónica V. Cunha and published by Humana Press. This book was released on 2014-11-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.
Download or read book Aspergillus Fumigatus and Aspergillosis written by Jean-Paul Latgé and published by . This book was released on 2009 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers the latest insights into the fundamental biology and pathogenesis of A. fumigatus. Provides a combined synopsis of both A. fumigatus and its diseases and therapies. Encompasses the most up-to-date knowledge to serve as a resource guide for the next decade of study on this organism and the many diseases it causes. Covers the fundamental biology of A. fumigatus including specific features in genetics, biochemistry, and cell biology that can explain the virulence of this opportunistic pathogen. Discusses the wide range of clinical infection, plus the latest diagnostic and treatment strategies, in specific patient populations.
Download or read book Oceans and Health written by Shimshon Belkin and published by Springer Science & Business Media. This book was released on 2006-10-12 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is surprising how little is actually known about the fate of wastewater bacteria once they enter the sea. This wide-ranging work is one of the first to unravel the mechanisms determining bacterial sensitivity or survival under these conditions.
