Download or read book Expanding Molecular Toolbox for Synthetic Biology written by Pawel Jajesniak and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advances in Synthetic Biology written by Vijai Singh and published by Springer Nature. This book was released on 2020-04-13 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the design of emerging conceptual tools, technologies and systems including novel synthetic parts, devices, circuits, oscillators, biological gates, and small regulatory RNAs (riboregulators and riboswitches), which serve as versatile control elements for regulating gene expression. Synthetic biology, a rapidly growing field that involves the application of engineering principles in biology, is now being used to develop novel systems for a wide range of applications including diagnostics, cell reprogramming, therapeutics, enzymes, vaccines, biomaterials, biofuels, fine chemicals and many more. The book subsequently summarizes recent developments in technologies for assembling synthetic genomes, minimal genomes, synthetic biology toolboxes, CRISPR-Cas systems, cell-free protein synthesis systems and microfluidics. Accordingly, it offers a valuable resource not only for beginners in synthetic biology, but also for researchers, students, scientists, clinicians, stakeholders and policymakers interested in the potential held by synthetic biology.

Download or read book Synthetic Biology written by Madan L. Nagpal and published by BoD – Books on Demand. This book was released on 2020-02-12 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic biology gives us a new hope because it combines various disciplines, such as genetics, chemistry, biology, molecular sciences, and other disciplines, and gives rise to a novel interdisciplinary science. We can foresee the creation of the new world of vegetation, animals, and humans with the interdisciplinary system of biological sciences. These articles are contributed by renowned experts in their fields. The field of synthetic biology is growing exponentially and opening up new avenues in multidisciplinary approaches by bringing together theoretical and applied aspects of science.

Download or read book Developing Multiplexed Molecular Assays for Synthetic Biology and DNA Data Storage with Nanopore Sensing Technology written by Nicolas Cardozo and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multiplexed molecular tools are a powerful means of interrogating biomolecular systems. Multiplexed assays offer significant advantages over singleplex assays, including time, reagent costs, sample requirements, and the amount of data that can be generated. In molecular biology, genetically encoded reporter proteins have expanded the toolbox of researchers to track biological phenomena. While they are widely used to measure many biological activities, the current number of uniquely addressable reporters that can be used together for one-pot multiplexed tracking is small due to overlapping detection channels such as fluorescence. Similarly, in DNA data storage, the ability to selectively target data files (i.e "randomly access") in a multiplexable manner would lessen decoding latency and cost and enable deployment of practical DNA data storage architectures. The primary focus of this dissertation is to develop new multiplexable molecular assays for synthetic biology circuits and DNA data storage random access readout using nanopore sensor arrays. To overcome genetically encoded reporter protein multiplexing limitations, we built an expanded library of orthogonally-barcoded Nanopore-addressable protein Tags Engineered as Reporters (NanoporeTERs), which can be read and demuxed by nanopore sensors at the single-molecule level. Subsequently, to improve upon previous random access architectures, we demonstrate a new random access approach in which files can be selected in multiplex using a CRISPR-Cas9 target address and then decoded using a nanopore sequencer. This work presents a new class of reporter proteins that permit multiplexed, real-time tracking of gene expression along with a new random access DNA data storage strategy that increases one-pot multiplexing and decreased time-to-decoding.

Download or read book Cell Free Synthetic Biology written by Jian Li and published by Frontiers Media SA. This book was released on 2022-01-13 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Metabolic Engineering written by Sang Yup Lee and published by John Wiley & Sons. This book was released on 2021-06-02 with total page 1075 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.

Download or read book Synthetic Biology written by Bernd Giese and published by Springer. This book was released on 2014-09-27 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic Biology is already an object of intensive debate. However, to a great extent the discussion to date has been concerned with fundamental ethical, religious and philosophical questions. By contrast, based on an investigation of the field’s scientific and technological character, this book focuses on new functionalities provided by synthetic biology and explores the associated opportunities and risks. Following an introduction to the subject and a discussion of the most central paradigms and methodologies, the book provides an overview of the structure of this field of science and technology. It informs the reader about the current stage of development, as well as topical problems and potential opportunities in important fields of application. But not only the science itself is in focus. In order to investigate its broader impact, ecological as well as ethical implications will be considered, paving the way for a discussion of responsibilities in the context of a field at a transitional crossroads between basic and applied science. In closing, the requirements for a suitable regulatory framework are discussed. The book is intended as a source of information and orientation for researchers, students and practitioners in the natural sciences and technology assessment; for members of scientific and technological, governmental and funding institutions; and for members of the general public interested in essential information on the current status, prospects and implications of synthetic biology.

Download or read book The Science and Applications of Synthetic and Systems Biology written by Institute of Medicine and published by National Academies Press. This book was released on 2011-12-30 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.

Download or read book Biodefense in the Age of Synthetic Biology written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2019-01-05 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific advances over the past several decades have accelerated the ability to engineer existing organisms and to potentially create novel ones not found in nature. Synthetic biology, which collectively refers to concepts, approaches, and tools that enable the modification or creation of biological organisms, is being pursued overwhelmingly for beneficial purposes ranging from reducing the burden of disease to improving agricultural yields to remediating pollution. Although the contributions synthetic biology can make in these and other areas hold great promise, it is also possible to imagine malicious uses that could threaten U.S. citizens and military personnel. Making informed decisions about how to address such concerns requires a realistic assessment of the capabilities that could be misused. Biodefense in the Age of Synthetic Biology explores and envisions potential misuses of synthetic biology. This report develops a framework to guide an assessment of the security concerns related to advances in synthetic biology, assesses the levels of concern warranted for such advances, and identifies options that could help mitigate those concerns.

Download or read book Synthetic Biology 2 Volumes written by Robert A. Meyers and published by John Wiley & Sons. This book was released on 2015-10-19 with total page 766 pages. Available in PDF, EPUB and Kindle. Book excerpt: These two volumes contain a selection of updated articles from the acclaimed Meyers Encyclopedia of Molecular Cell Biology and Molecular Medicine, the most authoritative resource in cell and molecular biology, combined with new articles by "founding fathers" in the field. The work is divided into six sections: + Biological Basis + Modeling + Modular Parts and Circuits + Synthetic Genomes + Diseases and Therapeutics + Chemicals Production. Ideally suited as advanced reading for students and postdocs, and with all current research trends covered by an impressive number of leading figures in the field, this is the first choice reference for research institutions.

Download or read book Synthetic Biology written by Christopher A. Voigt and published by Academic Press. This book was released on 2011 with total page 734 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic biology encompasses a variety of different approaches, methodologies and disciplines and many different definitions exist. This volume covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation.

Download or read book Advanced Fermentation and Cell Technology 2 Volume Set written by Byong H. Lee and published by John Wiley & Sons. This book was released on 2021-10-25 with total page 951 pages. Available in PDF, EPUB and Kindle. Book excerpt: ADVANCED FERMENTATION AND CELL TECHNOLOGY A comprehensive and up-to-date reference covering both conventional and novel industrial fermentation technologies and their applications Fermentation and cell culture technologies encompass more than the conventional microbial and enzyme systems used in the agri-food, biochemical, bioenergy and pharmaceutical industries. New technologies such as genetic engineering, systems biology, protein engineering, and mammalian cell and plant cell systems are expanding rapidly, as is the demand for sustainable production of bioingredients, drugs, bioenergy and biomaterials. As the growing biobased economy drives innovation, industrial practitioners, instructors, researchers, and students must keep pace with the development and application of novel fermentation processes and a variety of cell technologies. Advanced Fermentation and Cell Technology provides a balanced and comprehensive overview of the microbial, mammalian, and plant cell technologies used by the modern biochemical process industry to develop new and improved processes and products. This authoritative volume covers the essential features of advanced fermentation and cell technology, and highlights the interaction of food fermentation and cell culture biopharmaceutical actives. Detailed chapters, organized into five sections, cover microbial cell technology, animal and plant cell technology, safety issues of new biotechnologies, and applications of microbial fermentation to food products, chemicals, and pharmaceuticals. Written by an internationally-recognized expert in food biotechnology, this comprehensive volume: Covers both conventional and novel industrial fermentation technologies and their applications in a range of industries Discusses current progress in novel fermentation, cell culture, commercial recombinant bioproducts technologies Includes overviews of the global market size of bioproducts and the fundamentals of cell technology Highlights the importance of sustainability, Good Manufacturing Practices (GMP), quality assurance, and regulatory practices Explores microbial cell technology and culture tools and techniques such as genome shuffling and recombinant DNA technology, RNA interference and CRISPR technology, molecular thermodynamics, protein engineering, proteomics and bioinformatics, and synthetic biology Advanced Fermentation and Cell Technology is an ideal resource for students of food science, biotechnology, microbiology, agricultural sciences, biochemical engineering, and biochemistry, and is a valuable reference for food scientists, researchers, and technologists throughout the food industry, particularly the dairy, bakery, and fermented beverage sectors.

Download or read book Electroporation Protocols written by Shulin Li and published by Humana Press. This book was released on 2014-02-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

Download or read book Expanding The Chemical Space of RiPPs in Rare Actinobacetria Employing a Tunable Metabologenomic Approach written by Hamada Saad and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The intrinsic driving force of finding new RiPP scaffolds is largely attributed to their appreciable biological/physiological functions, however the constantly growing interest in disclosing novel architectures currently extends beyond the classical needs. For example, RiPP-BGCs regularly provide a rich pool of enzymatic machineries that can install specific, in some cases rare, tailorings expanding the biobrick toolbox in synthetic biology and chemistry. Although the analyses of different genome sequences of Nocardia spp. disclosed a huge cryptic biosynthetic potential waiting to be unearthed, the discovery of such genetically encoded entities is insurmountable. Thus, to partially overcome the typical limitations offered by the common silence of biosynthetic gene clusters (BGCs) and the inherited challenge to connect the genotype(s) with the chemotype(s), a tunable metabologenomic approach was developed within the frame of this work. The application of the OSMAC concept in tandem with the configured analytical strategy in Nocardia terpenica IFM 0406, 0706T and Longimycelium tulufanense CGMCC 4.5737 enabled to chart unprecedented chemical space belonging to RiPPs with three novel molecular families. The first study case comprises a genome-oriented prioritization of a novel BGC associated with a new-to-nature combination of multiple post-translational enzymes in N. terpenica IFM 0406. It was hypothesized that the gene cluster will in turn assemble a novel chemical entity blending three different class-defining post-translational modifications (PTMs). Recruiting a synergism of bioinformatics, stable isotope labeling experiments and NMR spectroscopy facilitated the successful identification, isolation and structural characterization of three new RiPPs, named nocathioamides A-C. Aside from structurally featuring unprecedented PTM in the form of a macrocyclic imide bridge, they inaugurate the first-in-nature combinatorial tribrid RiPPs hovering over three different biosynthetic machineries of lanthipeptides, LAPs and thioamitides. The second investigation deals with the deorphanization of an additional unique RiPP-BGC found in N. terpenica genomes. The bioinformatic annotation and prediction pinpoint to an unknown scaffold belonging to class II lasso peptide which is additionally decorated with a possible oxidative event(s). A quick bioinformatic survey revealed the presence of homologous BGCs coding for similar entities in other bacteria exemplified by Longimycelium tulufanense. Relying on the predicted core peptides in tandem with the tuned workflow, the encoded products were deciphered from both isolates, termed nocapeptins and longipeptins. Nocapeptins, from N. terpenica, and longipeptins, from L. tulufanense, symbolize the founders of a novel molecular family of lasso peptides in which a conserved regional PTM as a unique oxidative crosslink is structurally appended. Aside from the characteristic skeletal inter-linkage embedded in longipeptin A, the structure could leverage the modification scope with further tailoring events like hydroxylation and methylation, delivering thereby the most tailored lasso architecture till now. The discovery of nocathioamides and nocapeptins stretched with longipeptins portrays a proof of concept to the effectiveness of employing the technique of genome mining prioritization to unearth a new chemical space with uncharted biosynthetic enzymes from underexplored species.

Download or read book Progress in Mycology written by Tulasi Satyanarayana and published by Springer Nature. This book was released on 2022-02-22 with total page 676 pages. Available in PDF, EPUB and Kindle. Book excerpt: Indian mycologists have extensively studied various groups of fungi such as soil fungi, aquatic fungi, marine fungi, endophytic fungi, fungi associated with man and animals. Though several books on various aspects of fungi are published, this is the first account of the history and development in mycology in India. This book is the second volume of the two-volume book "Progress in Mycology: An Indian Perspective". While volume 1 contains the historical aspects, taxonomy and information about the various groups of fungi, this volume focuses majorly on the biotechnological applications of the different groups of fungi. It discusses topics such as the extremophilic fungi, the history and development in Candida research, progress of mycotoxin research in India etc. It provides a detailed account of the various enzymes and bio-active molecules derived from fungi. India shows a very high biodiversity of fungi, and this book discusses these different group of fungi and their industrial and biotechnological applications. This book is useful to students, teachers and researchers in botany, microbiology, biotechnology and life sciences, agriculture and industries using fungi to produce various valuable products.

Download or read book Plant Genomes written by Jean-Nicolas Volff and published by Karger Medical and Scientific Publishers. This book was released on 2008-01-01 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent major advances in the field of comparative genomics and cytogenomics of plants, particularly associated with the completion of ambitious genome projects, have uncovered astonishing facets of the architecture and evolutionary history of plant genomes. The aim of this book was to review these recent developments as well as their implications in our understanding of the mechanisms which drive plant diversity. New insights into the evolution of gene functions, gene families and genome size are presented, with particular emphasis on the evolutionary impact of polyploidization and transposable elements. Knowledge on the structure and evolution of plant sex chromosomes, centromeres and microRNAs is reviewed and updated. Taken together, the contributions by internationally recognized experts present a panoramic overview of the structural features and evolutionary dynamics of plant genomes.This volume of Genome Dynamics will provide researchers, teachers and students in the fields of biology and agronomy with a valuable source of current knowledge on plant genomes.

Download or read book New Frontiers and Applications of Synthetic Biology written by Vijai Singh and published by Academic Press. This book was released on 2022-01-12 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Frontiers and Applications of Synthetic Biology presents a collection of chapters from eminent synthetic biologists across the globe who have established experience and expertise working with synthetic biology. This book offers several important areas of synthetic biology which allow us to read and understand easily. It covers the introduction of synthetic biology and design of promoter, new DNA synthesis and sequencing technology, genome assembly, minimal cells, small synthetic RNA, directed evolution, protein engineering, computational tools, de novo synthesis, phage engineering, a sensor for microorganisms, next-generation diagnostic tools, CRISPR-Cas systems, and more. This book is a good source for not only researchers in designing synthetic biology, but also for researchers, students, synthetic biologists, metabolic engineers, genome engineers, clinicians, industrialists, stakeholders and policymakers interested in harnessing the potential of synthetic biology in many areas. Offers basic understanding and knowledge in several aspects of synthetic biology Covers state-of-the-art tools and technologies of synthetic biology, including promoter design, DNA synthesis, DNA sequencing, genome design, directed evolution, protein engineering, computational tools, phage design, CRISPR-Cas systems, and more Discusses the applications of synthetic biology for smart drugs, vaccines, therapeutics, drug discovery, self-assembled materials, cell free systems, microfluidics, and more