Download or read book Enzyme Catalysis in Water organic Solvent Two phase Systems written by Rolf Andrew Ramelmeier and published by . This book was released on 1989 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Design of Enzymatic Systems for Catalysis in Organic Solvents written by Valerie Mei-lin Reiko Suzawa and published by . This book was released on 1995 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme Design for Nonaqueous Media Optimization of Enzymatic Catalysis for Organic Solvent Systems written by and published by . This book was released on 1994 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: From a biotechnological perspective there are many potential advantages of employing enzymes in organic as opposed to aqueous media. To that end, we have concluded an initial three-year research program in the area of enzymatic catalysis in organic solvents. Our studies have focused on the effect of protein hydration on subtilisins BPN' and Carlsberg in nonaqueous media. Investigations on protein engineered mutants, catalyst engineering studies, and structural studies, primarily employing EPR spectroscopy, have revealed fundamental information on the role of water, the the nature of enzyme structure, and the effects of solvents on the catalytic activity in organic solvents. This study has also resulted in several rational methods to dramatically improve enzyme activity under anhydrous conditions. Catalytic activities in organic solvents can now be expressed at levels similar to that in water--this is a major advance in the field of nonaqueous enzymology. (jg).
Download or read book Enzyme Catalysis in Organic Synthesis written by Karlheinz Drauz and published by Wiley-VCH. This book was released on 2002-07-26 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: Organic synthesis with enzymes - the only way This comprehensive set gives reliable answers to all questions on enzyme catalysis - from searching for suitable catalytic systems via choosing the optimal reaction conditions to implementing modern synthesis strategies. The long-awaited new edition has been greatly expanded to include new topics and to reflect the latest research, yet retains the clear and practice-oriented presentation found in the first edition. More than a mere data collection, the three volumes provide synthetic chemists with easy access to all the first-hand information necessary for successfully using enzymes: - the latest synthesis methods - example applications arranged according to reaction type - a table of all the important, commercially available enzymes - comprehensive registers for targeted searching according to enzyme, compound, or reaction - current references to the literature In short, an indispensable reference that should be on the shelf of every modern synthesis laboratory.
Download or read book Direct Evolution Process for Robust Enzyme Catalysis in Organic Solvents written by and published by . This book was released on 1996 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: Industrial applications of enzymes have been limited by the availability of robust, reliable and economical protein-based catalysts. The goal of the work outlined in this proposal was to continue development and commercialization of an iterative process to rapidly evolve sequence variants of selected enzymes with enhanced industrial potential. This directed evolution approach involves the use of random mutagenic techniques followed by activity screening of thousands of resulting mutant clones under selected assay conditions. Screening for activity in successively higher concentrations of a selected solvent following sequential rounds of mutagenesis results in the accumulation of amino acid substitutions which synergistically contribute to optimum activity. Phase I results of this work have shown that a percentage of clones expressing the serine protease subtilisin, when subjected to error-prone PCR mutagenesis, and subsequently screened for improved ligase activity in dimethylformamide (DMF), produced enzyme which catalyzed the polymerization of amino acids into extended peptides.
Download or read book Extractive Catalysis written by Daryl Kevin Eggers and published by . This book was released on 1987 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Multi Step Enzyme Catalysis written by Eduardo Garcia-Junceda and published by John Wiley & Sons. This book was released on 2008-11-21 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first comprehensive coverage of this unique and interdisciplinary field provides a complete overview, covering such topics as chemoenzymatic synthesis, microbial production of DNA building blocks, asymmetric transformations by coupled enzymes and much more. By combining enzymatic and synthetic organic steps, the use of multi-enzyme complexes and other techniques opens the door to reactions hitherto unknown, making this monograph of great interest to biochemists, organic chemists, and chemists working with/on organometallics, as well as catalytic chemists, biotechnologists, and those working in the pharmaceutical and fine chemical industries.
Download or read book Catalysis and Enzyme Action written by Myron L. Bender and published by McGraw-Hill Companies. This book was released on 1973 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme catalysed Reactions written by Charles John Gray and published by Van Nostrand Reinhold Company. This book was released on 1971 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymic and Non enzymic Catalysis written by Peter Dunnill and published by Ellis Horwood. This book was released on 1980 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme catalysis in organic synthesis a comprehensive handbook 2 written by Karlheinz Drauz and published by . This book was released on 2002 with total page 1559 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme Catalysis in Organic Synthesis written by Karlheinz Drauz and published by . This book was released on 2012 with total page 1985 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymatic Interesterification in Supercritical Carbon Dioxide written by Douglas Alan Miller and published by . This book was released on 1990 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Non Aqueous Enzymology written by Munishwar N. Gupta and published by Birkhäuser. This book was released on 2013-12-01 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.
Download or read book Modern Biocatalysis written by Gavin Williams and published by Royal Society of Chemistry. This book was released on 2018-05-31 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: The synergy between synthetic biology and biocatalysis is emerging as an important trend for future sustainable processes. This book reviews all modern and novel techniques successfully implemented in biocatalysis, in an effort to provide better performing enzymatic systems and novel biosynthetic routes to (non-)natural products. This includes the use of molecular techniques in protein design and engineering, construction of artificial metabolic pathways, and application of computational methods for enzyme discovery and design. Stress is placed on current ‘hot’ topics in biocatalysis, where recent advances in research are defining new grounds in enzyme-catalyzed processes. With contributions from leading academics around the world, this book makes a ground-breaking contribution to this progressive field and is essential reading for graduates and researchers investigating (bio)catalysis, enzyme engineering, chemical biology, and synthetic biology.
Download or read book Enzymes in Nonaqueous Solvents written by Evgeny N. Vulfson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 677 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic catalysis has gained considerable attention in recent years as an efficient tool in the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients. The high selectivity and mild reaction con- tions associated with enzymatic transformations have made this approach an attractive alternative in the synthesis of complex bioactive compounds, which are often difficult to obtain by standard chemical routes. However, the maj- ity of organic compounds are not very soluble in water, which was traditi- ally perceived as the only suitable reaction medium for the application of biocatalysts. The realization that most enzymes can function perfectly well under nearly anhydrous conditions and, in addition, display a number of useful properties, e. g. , highly enhanced stability and different selectivity, has d- matically widened the scope of their application to the organic synthesis. Another great attraction of using organic solvents rather than water as a reaction solvent is the ability to perform synthetic transformations with re- tively inexpensive hydrolytic enzymes. It is worth reminding the reader that in vivo, the synthetic and hydrolytic pathways are catalyzed by different enzymes. However, elimination of water from the reaction mixture enables the “reversal” of hydrolytic enzymes and thus avoids the use of the expensive cofactors or activated substrates that are required for their synthetic count- parts.
Download or read book Enzymology and Enzyme Technology written by Bhatt S.M. and published by S. Chand Publishing. This book was released on 2022-01-03 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: discussion of the correction between the structure and properties of elements/ compound. The book caters to the requirements of Bachelor in Science (Pass) courses. With detailed discussion on several advanced topics, the students of Bachelor in Science (Honours) and Masters in Science would also find it extremely
