Download or read book Engineering Bio inspired Microenvironments for Stem Cell Proliferation and Skeletal Differentiation written by Yongsung Hwang and published by . This book was released on 2011 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interactions of cells with their extracellular matrix (ECM) play an important role in development, disease progression, and regeneration. The material properties of ECM such as geometry, chemistry, hydrophobicity, mechanics, and microstructure play an important role in regulating various cellular processes and tissue morphogenesis. Thus there is a surge of interest in determining the effect of various material properties on cell fate and tissue formation. Beyond providing fundamental understandings, these efforts can also led to development of effective scaffolds (structural support for cell culture) as scaffold engineering is an integral part of in vitro cell cultures, tissue engineering, and transplants/implants. The central focus of this thesis is design of polymer-based biomaterials as synthetic matrices for cell culture and understands how cell-matrix interactions affect tissue formation, stem cell differentiation and self-renewal. The first two chapters focuses on synthesis and characterization of three dimensional hydrogels having interconnected macroporous network structures of poly(ethylene glycol) using cryogelation techniques, where I have developed a novel, green strategy to create monolithic structures with heterogeneous and homogenous networks. I have also developed a process to create three-dimensional structures with different internal architecture while maintaining the same porosity. These 3D structures were then used to understand effect of scaffold porosity, pore structure, etc play an important role in cell proliferation, differentiation, and tissue formation using cartilage tissue engineering as a model system. We also harnessed the potential of cell-matrix interactions to develop defined, synthetic matrices to expand human embryonic stem cells in vitro without introducing any detrimental effects. Employing a number of hydrogels I have determined the effect of various physico-chemical cues (bulk and interfacial properties) on adhesion, growth, colony formation, and self-renewal of human embryonic stem cells. Poly[acrylamide-co-sodium 4-vinylbenzenesulfonate] hydrogels having a moderate hydrophobicity (water contact angle of 23°) and bulk rigidity of 343.7kPa have supported in vitro growth of a number of hPSCs (HUES9, HUES6, and iPSCs) in defined medium (StemPro®) for more than 20 passages. These studies are discussed in chapter 4.

Download or read book Engineering Stem Cells For Tissue Regeneration written by Ngan Huang and published by World Scientific. This book was released on 2017-11-24 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering integrates knowledge and tools from biological sciences and engineering for tissue regeneration. A challenge for tissue engineering is to identify appropriate cell sources. The recent advancement of stem cell biology provides enormous opportunities to engineer stem cells for tissue engineering. The impact of stem cell technology on tissue engineering will be revolutionary.This book covers state-of-the-art knowledge on the potential of stem cells for the regeneration of a wide range of tissues and organs, including cardiovascular, musculoskeletal, neurological and skin tissues. The technology platforms for studying and engineering stem cells, such as hydrogel and biomaterials development, microfluidics system and microscale patterning, are also illustrated. Regulatory challenges and quality control for clinical translation are also detailed. This book provides an comprehensive update on the advancement in the field of stem cells and regenerative medicine, and serves as a valuable resource for both researchers and students.

Download or read book Bio inspired Materials for Biomedical Engineering written by Anthony B. Brennan and published by John Wiley & Sons. This book was released on 2014-04-14 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the latest bio-inspired materials synthesis techniques and biomedical applications that are advancing the field of tissue engineering. Bio-inspired concepts for biomedical engineering are at the forefront of tissue engineering and regenerative medicine. Scientists, engineers and physicians are working together to replicate the sophisticated hierarchical organization and adaptability found in nature and selected by evolution to recapitulate the cellular microenvironment. This book demonstrates the dramatic clinical breakthroughs that have been made in engineering all four of the major tissue types and modulating the immune system. Part I (Engineering Bio-inspired Material Microenvironments) covers Bio-inspired Presentation of Chemical Cues, Bio-inspired Presentation of Physical Cues, and Bio-inspired Integration of Natural Materials. Part II (Bio-inspired Tissue Engineering) addresses tissue engineering in epithelial tissue, muscle tissue, connective tissue, and the immune system.

Download or read book Stem Cell Bioengineering and Tissue Engineering Microenvironment written by Satya Prakash and published by World Scientific. This book was released on 2011 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive review of the current state of stem cell bioengineering from authorities in the field. The first part of the book includes the basic research work on stem cells and bioengineering carried out by various laboratories. The second part consists of a review of the current development of various microcapsules in stem cell therapy. The last part will summarize the overall clinical trials on stem cell therapy and myocardial regeneration as well as the most updated personal experience recently completed by well-known experts in this field.

Download or read book Engineering Materials for Stem Cell Regeneration written by Faheem A. Sheikh and published by Springer Nature. This book was released on 2021-10-23 with total page 709 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the interface of stem cell biology and biomaterials for regenerative medicine. It presents the applications of biomaterials to support stem cell growth and regeneration. The book discusses the stem cell interactions’ with nanofiber, gradient biomaterial, polymer- and ceramic biomaterials, integrating top-down and bottom-up approaches, adhesive properties of stem cells on materials, cell-laden hydrogels, micro-and nanospheres, de-cellularization techniques, and use of porous scaffolds. Further, this book provides a basic introduction to the fabrication techniques for creating various biomaterials that can be used for stem cell differentiation. It also elucidates the properties of stem cells, their characteristic features, tissue culture technology, properties of pluripotency, osteogenesis, and biomaterial interaction with de-cellularized organs, cell lineage in vivo and in vitro, gene expression, embryonic development, and cell differentiation. Further, the book reviews the latest applications of bio-instructive scaffold for supporting stem cell differentiation and tissue regeneration. The book also presents stem cell for dental, alveolar bone and cardiac regeneration. Lastly, it introduces engineered stem cells for delivering small molecule therapeutics and their potential biomedical applications.

Download or read book Biomaterials as Stem Cell Niche written by Krishnendu Roy and published by Springer Science & Business Media. This book was released on 2010-09-30 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in stem cell biology have opened new directions in cell therapy. This book provides the state-of-the-art developments in using biomaterials as artificial niches for engineering stem cells, both for the purpose of better understanding their biology under 3D biomimetic conditions as well as for developing new strategies for efficient long term maintenance and directed differentiation of stem cells into various therapeutic lineages. Animal and human stem cells of both embryonic and adult origin are discussed with applications ranging from nerve regeneration, orthopedics, cardiovascular therapy, blood cell generation and cancer therapy. Both synthetic and natural biomaterials are reviewed with emphasis on how material-stem cell interactions direct specific signaling pathways and ultimately modulate the cell fate. This book is valuable for biomaterial scientists, tissue engineers, clinicians as well as stem cell biologists involved in basic research and applications of adult and embryonic stem cells.

Download or read book Stem Cell Engineering written by David Schaffer and published by CRC Press. This book was released on 2012-11-20 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the potential of stem cells is recognized, their proliferation and differentiation must be more precisely controlled to maximize the production of therapeutically relevant cells and for cell replacement therapies to minimize contamination with residual cells that can give rise to side effects. How can engineers make contributions to address these challenges? With contributions from pioneers and experts, Stem Cell Engineering: Principles and Practices highlights recent advances in the understanding of the cellular and molecular composition of the stem cell niche, as well as approaches to build upon this basic information to direct stem cell differentiation into therapeutically valuable lineages. The growing recognition of stem cells as an important and exciting field will continue to draw investigators with diverse backgrounds—from biology, engineering, and the physical sciences—and thereby enable further progress in these and other new directions. This book discusses advances made during the last decade that have led to increasingly defined culture systems for growing stem cells, starting from co-culture with feeder cells in the presence of serum to growth on synthetic substrates in defined medium. In addition to highlighting many recent advances, it underscores the need for future work.

Download or read book Stem Cell Microenvironments and Beyond written by Alexander Birbrair and published by Springer. This book was released on 2017-12-03 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the main stem cell niches under distinct pathophysiological conditions. The role of tissue microenvironments in stem cell regulation, as well as modern methodologies and new techniques for the identification and characterization of stem cell niches, are discussed by leading experts in the field. Chapters describe the major components of various stem cell microenvironments, such as cellular components, soluble factors, cell-cell interactions, extra-cellular matrix proteins, and physical forces. Stem Cell Microenvironments and Beyond is part of the highly successful Advances in Experimental Medicine and Biology series. It is essential reading for graduate students and researchers in the field of stem cells or cell biology as well as clinicians.

Download or read book Advanced Surfaces for Stem Cell Research written by Ashutosh Tiwari and published by John Wiley & Sons. This book was released on 2016-11-29 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book outlines first the importance of Extra Cellular Matrix (ECM), which is a natural surface for most of cells. In the following chapters the influence of biological, chemical, mechanical, and physical properties of surfaces in micro and nano-scale on stem cell behavior are discussed including the mechanotransduction. Biomimetic and bioinspired approaches are highlighted for developing microenvironment of several tissues, and surface engineering applications are discussed in tissue engineering, regenerative medicine and different type of biomaterials in various chapters of the book. This book brings together innovative methodologies and strategies adopted in the research and development of Advanced Surfaces in Stem Cell Research. Well-known worldwide researchers deliberate subjects including: Extracellular matrix proteins for stem cell fate The superficial mechanical and physical properties of matrix microenvironment as stem cell fate regulator Effects of mechanotransduction on stem cell behavior Modulation of stem cells behavior through bioactive surfaces Influence of controlled micro and nanoengineered surfaces on stem cell fate Nanostructured polymeric surfaces for stem cells Laser surface modification techniques and stem cells applications Plasma polymer deposition: a versatile tool for stem cell research Application of bioreactor concept and modeling techniques in bone regeneration and augmentation treatments Substrates and surfaces for control of pluripotent stem cell fate and function Application of biopolymer-based, surface modified devices in transplant medicine and tissue engineering Silk as a natural biopolymer for tissue engineering

Download or read book Stem Cells and Tissue Engineering written by Mirjana Pavlovic and published by Springer Science & Business Media. This book was released on 2012-11-07 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cells are the building blocks for all other cells in an organism. The human body has about 200 different types of cells and any of those cells can be produced by a stem cell. This fact emphasizes the significance of stem cells in transplantational medicine, regenerative therapy and bioengineering. Whether embryonic or adult, these cells can be used for the successful treatment of a wide range of diseases that were not treatable before, such as osteogenesis imperfecta in children, different forms of leukemias, acute myocardial infarction, some neural damages and diseases, etc. Bioengineering, e.g. successful manipulation of these cells with multipotential capacity of differentiation toward appropriate patterns and precise quantity, are the prerequisites for successful outcome and treatment. By combining in vivo and in vitro techniques, it is now possible to manage the wide spectrum of tissue damages and organ diseases. Although the stem-cell therapy is not a response to all the questions, it provides more and more answers every day. Stem Cells and Tissue Engineering is a concise review on the functional, phenotypic, regenerative, transplantational and curative aspects of a stem cell’s entity. It is critical and encouraging at the same time, providing truthful and appropriate samples from the practice and research that can lead toward optimal use of this immense source of adjuvant and curative therapy in human pathology. Written by a clinician and a researcher, who are currently teaching what they are doing, it is recommended as a teaching tool along with an original textbook.

Download or read book Biomaterials and Bioactive Molecules to Drive Differentiation in Striated Muscle Tissue Engineering written by Valentina Di Felice and published by Frontiers Media SA. This book was released on 2016-05-18 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering is an innovative, multidisciplinary approach which combines (bio)materials, cells and growth factors with the aim to obtain neo-organogenesis to repair or replenish damaged tissues and organs. The generation of engineered tissues and organs (e. g. skin and bladder) has entered into the clinical practice in response to the chronic lack of organ donors. In particular, for the skeletal and cardiac muscles the translational potential of tissue engineering approaches has clearly been shown, even though the construction of this tissue lags behind others given the hierarchical, highly organized architecture of striated muscles. Cardiovascular disease is the leading cause of death in the developed world, where the yearly incidence of Acute MI (AMI) is approx 2 million cases in Europe. Recovery from AMI and reperfusion is still less than ideal. Stem cell therapy may represent a valid treatment. However, delivery of stem cells alone to infarcted myocardium provides no structural support while the myocardium heals, and the injected stem cells do not properly integrate into the myocardium because they are not subjected to the mechanical forces that are known to drive myocardial cellular physiology. On the other hand, there are many clinical cases where the loss of skeletal muscle due to a traumatic injury, an aggressive tumour or prolonged denervation may be cured by the regeneration of this tissue. In vivo, stem or progenitor cells are sheltered in a specialized microenvironment (niche), which regulates their survival, proliferation and differentiation. The goal of this research topic is to highlight the available knowledge on biomaterials and bioactive molecules or a combination of them, which can be used successfully to differentiate stem or progenitor cells into beating cardiomyocytes or organized skeletal muscle in vivo. Innovations compared to the on-going trials may be: 1) the successful delivery of stem cells using sutural scaffolds instead of intracoronary or intramuscular injections; 2) protocols to use a limited number of autologous or allogeneic stem cells; 3) methods to drive their differentiation by modifying the chemical-physical properties of scaffolds or biomaterials, incorporating small molecules (i.e. miRNA) or growth factors; 4) methods to tailor the scaffolds to the elastic properties of the muscle; 5) studies which suggest how to realize scaffolds that optimize tissue functional integration, through the combination of the most up-to-date manufacturing technologies and use of bio-polymers with customized degradation properties.

Download or read book Micro and Nanotechnologies in Engineering Stem Cells and Tissues written by Murugan Ramalingam and published by John Wiley & Sons. This book was released on 2013-05-10 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: A cutting-edge look at the application of micro and nanotechnologies in regenerative medicine The area at the interface of micro/nanotechnology and stem cells/tissue engineering has seen an explosion of activity in recent years. This book provides a much-needed overview of these exciting developments, covering all aspects of micro and nanotechnologies, from the fundamental principles to the latest research to applications in regenerative medicine. Written and edited by the top researchers in the field, Micro and Nanotechnologies in Engineering Stem Cells and Tissues describes advances in material systems along with current techniques available for cell, tissue, and organ studies. Readers will gain tremendous insight into the state of the art of stem cells and tissue engineering, and learn how to use the technology in their own research or clinical trials. Coverage includes: Technologies for controlling or regulating stem cell and tissue growth Various engineering approaches for stem cell, vascular tissue, and bone regeneration The design and processing of biocompatible polymers and other biomaterials Characterization of the interactions between cells and biomaterials Unrivaled among books of this kind, Micro and Nanotechnologies in Engineering Stem Cells and Tissues is the ultimate forward-looking reference for researchers in numerous disciplines, from engineering and materials science to biomedicine, and for anyone wishing to understand the trends in this transformative field.

Download or read book Stem Cell Biology and Regenerative Medicine written by Mehdi Razavi and published by Bentham Science Publishers. This book was released on 2017-10-02 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering is an interdisciplinary field which involves the fabrication of tissues by using a porous protein scaffold, cells and bioactive molecules. The aim of tissue engineering is to enhance medical applications and procedures. This volume introduces the reader to the basics of employing pluripotent stem cells in tissue engineering. Successive chapters present knowledge about relevant tissue engineering techniques in different medical and physiological specialties including dentistry, cardiovascular physiology, hepatology, nephrology, dermatology and orthopedics. The text offers a wealth of information that will be of use to all students, bioengineers, materials scientists, chemists, physicians and surgeons concerned with the properties, performance, and the application of tissue engineering scaffolds in clinical settings.

Download or read book Bioreactor Systems for Tissue Engineering II written by Cornelia Kasper and published by Springer. This book was released on 2010-10-17 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alternative Sources of Adult Stem Cells: Human Amniotic Membrane, by S. Wolbank, M. van Griensven, R. Grillari-Voglauer, and A. Peterbauer-Scherb; * Mesenchymal Stromal Cells Derived from Human Umbilical Cord Tissues: Primitive Cells with Potential for Clinical and Tissue Engineering Applications, by P. Moretti, T. Hatlapatka, D. Marten, A. Lavrentieva, I. Majore, R. Hass and C. Kasper; * Isolation, Characterization, Differentiation, and Application of Adipose-Derived Stem Cells, by J. W. Kuhbier, B. Weyand, C. Radtke, P. M. Vogt, C. Kasper and K. Reimers; * Induced Pluripotent Stem Cells: Characteristics and Perspectives, by T. Cantz and U. Martin; * Induced Pluripotent Stem Cell Technology in Regenerative Medicine and Biology, by D. Pei, J. Xu, Q. Zhuang, H.-F. Tse and M. A. Esteban; * Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells, by C. Weber, S. Pohl, R. Poertner, P. Pino-Grace, D. Freimark, C. Wallrapp, P. Geigle and P. Czermak; * Cartilage Engineering from Mesenchymal Stem Cells, by C. Goepfert, A. Slobodianski, A.F. Schilling, P. Adamietz and R. Poertner; * Outgrowth Endothelial Cells: Sources, Characteristics and Potential Applications in Tissue Engineering and Regenerative Medicine, by S. Fuchs, E. Dohle, M. Kolbe, C. J. Kirkpatrick; * Basic Science and Clinical Application of Stem Cells in Veterinary Medicine, by I. Ribitsch, J. Burk, U. Delling, C. Geißler, C. Gittel, H. Jülke, W. Brehm; * Bone Marrow Stem Cells in Clinical Application: Harnessing Paracrine Roles and Niche Mechanisms, by R. M. El Backly, R. Cancedda; * Clinical Application of Stem Cells in the Cardiovascular System, C. Stamm, K. Klose, Y.-H. Choi

Download or read book Stem Cell And Tissue Engineering written by Song Li and published by World Scientific. This book was released on 2011-01-14 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering integrates knowledge and tools from biological sciences and engineering for tissue regeneration. A challenge for tissue engineering is to identify appropriate cell sources. The recent advancement of stem cell biology provides enormous opportunities to engineer stem cells for tissue engineering. The impact of stem cell technology on tissue engineering will be revolutionary. This book covers state-of-the-art knowledge on the potential of stem cells for the regeneration of a wide range of tissues and organs and the technologies for studying and engineering stem cells. It serves as a valuable reference book for researchers and students.

Download or read book Genetic Engineering of Mesenchymal Stem Cells written by Jan A. Nolta and published by Springer Science & Business Media. This book was released on 2006-08-29 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: MSC (mesenchymal stem cells) have been reported to initiate revascularization after injury, to facilitate engraftment of blood-forming stem cells, and to reduce the incidence of graft-vs. host disease through their immune-suppressive qualities. Finally, bone marrow-derived MSC have been reported to home to areas of solid tumor revascularization, and thus may be used as delivery vehicles to target ablative agents into dividing tumor cells. Recently the characteristics of human MSC from adipose (fat) tissue have also been identified. The possibility of repairing tissues, speeding stem cell engraftment, and targeting solid tumors for specific killing, using MSC easily harvested from bone marrow, or better yet, from unwanted fat tissue, holds broad appeal, and is an intriguing possibility that could have dramatic effect on health care. This book has information on how to isolate, grow, and characterize MSC from marrow and fat, and gives important insight into how these cells may be used for gene delivery and cellular therapies in the future. Updates on emerging clinical trials are given.

Download or read book Cell Inspired Materials and Engineering written by Dan Ohtan Wang and published by Springer. This book was released on 2022-04-16 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights cutting-edge studies in the development of cell-inspired biomaterials and synthetic materials that manipulate cell functions and provide the next generation with contemporary tools for treating complex human diseases. It explores the convergence of synthetic materials with cell and molecular biology and surveys how functional materials, when patterned with spatial and temporal precision, can be used effectively to maintain cell proliferation and phenotype in vitro, to trigger specific cell functions, and to redirect cell-fate decisions. Human stem cells are a frequently discussed subject in this book. This is an ideal book for students, cell biologists, researchers interested in interdisciplinary research, and biomedical engineers. This book also: Highlights successfully developed technologies in cell engineering that make possible new therapeutic development for previously untreatable conditions Covers topics including bio-inspired micro patterning, DNA origami technology, synthetic NOS inspired by compartmentalized signaling in cells, and light-induced depolarization of the cell membrane Illustrates in detail the use of stem cells and synthetic scaffolds to model ethically sensitive embryonic tissues and organs