Download or read book Embryo Culture written by Gary D. Smith and published by Humana Press. This book was released on 2016-05-01 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology book covers the complete range of contemporary methods for the study of human embryo culture. Includes lists of necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and pitfalls."
Download or read book Textbook of Clinical Embryology written by Kevin Coward and published by Cambridge University Press. This book was released on 2013-10-31 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
Download or read book Embryo Culture written by Beth Kohl and published by Macmillan. This book was released on 2007 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this marvelously unconventional account of her struggles to bear children, Kohl leads the reader on an up-close tour of fertilization in America, and the ways in which science and miracle, technology and faith, converge to create life in the 21st century.
Download or read book Manual of Embryo Culture in Human Assisted Reproduction written by Kersti Lundin and published by Cambridge University Press. This book was released on 2021-05-06 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whilst assisted reproduction techniques (ART) have become increasingly successful and largely standardized, there is still only a partial understanding of what constitutes a 'true' embryo environment. Replicating the varying physiological conditions of the in-vivo environment that the embryo travels through in the in-vitro culture is still a major challenge in ART. This practical volume details how to organize and operate an IVF laboratory in order to mimic these conditions for successful embryo culture. Environments and equipment that are essential for running safe and efficient facilities such as maintaining good air quality and hygiene protocols, and utilizing an effective layout are covered in detail. Other chapters discuss the different consumables needed, optimal handling techniques and parameter monitoring systems, as well as recent advances in the area including artificial intelligence and automation. This is an indispensable guide to understanding the background science of culturing embryos, crucial to successful outcomes in ART.
Download or read book Plant Embryo Culture written by Trevor A. Thorpe and published by Humana Press. This book was released on 2011-01-07 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular BiologyTM series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.
Download or read book Culture Media Solutions and Systems in Human ART written by Patrick Quinn and published by Cambridge University Press. This book was released on 2014-03-27 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.
Download or read book Human Assisted Reproductive Technology written by David K. Gardner and published by Cambridge University Press. This book was released on 2011-03-31 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Assisted Reproductive Technology: Future Trends in Laboratory and Clinical Practice offers a collection of concise, practical review articles on cutting-edge topics within reproductive medicine. Each article presents a balanced view of clinically relevant information and looks ahead to how practice will change over the next five years. The clinical section discusses advances in reproductive surgery and current use of robotic surgery for tubal reversal and removal of fibroids. It looks into the refinement of surgical procedures for fertility preservation purposes. Chapters also discuss non-invasive diagnosis of endometriosis with proteomics technology, new concepts in ovarian stimulation and in the management of polycystic ovary syndrome, and evidence-based ART. The embryology section discusses issues ranging from three-dimensional in-vitro ovarian follicle culture, and morphometric and proteomics analysis of embryos, to oocyte and embryo cyropreservation. This forward-looking volume of review articles is key reading for reproductive medicine physicians, gynecologists, reproductive endocrinologists, urologists and andrologists.
Download or read book A Practical Guide to Setting Up an IVF Lab Embryo Culture Systems and Running the Unit written by Alex C Varghese and published by JP Medical Ltd. This book was released on 2013-07-30 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a complete guide to setting up an IVF laboratory. Beginning with an introduction to the history and the basics, the following chapters take clinicians through the full set up and management process, from air quality control and cryopreservation facilities, to morphological embryo assessment, sperm processing and selection techniques, to document management systems. A separate chapter provides an update on semen analysis based on World Health Organisation (WHO) standards and interpretation of results. Written by an extensive author and editor team from the UK, Europe and the USA, this practical manual is invaluable for embryologists and IVF specialists planning to set up and manage an IVF laboratory successfully. Key points Practical guide to setting up and managing an IVF laboratory Provides step by step process Includes chapter on semen analysis based on WHO standards and interpretation of results Extensive author and editor team from UK, Europe and USA
Download or read book In Vitro Embryogenesis in Plants written by Trevor A. Thorpe and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: In vitro Embryogenesis in Plants is the first book devoted exclusively to this topic. As the ultimate demonstration of totipotency in plants, somatic and haploid embryogenesis is of vital importance to all those working on or interested in basic and applied aspects of plantlet information and regeneration. The text includes comprehensive reviews written by experts, on all facts of in vitro and in vivo embryogenesis. Some chapters deal with the morphogenic, structural and developmental, physiological and biochemical, and molecular biological aspects of the subject. Chapters are also devoted to haploid embryogenesis, asexual embryogenesis in nature, zygotic embryogenesis, and zygotic embryo culture. Detailed tables summarizing successful somatic embryogenesis in all vascular plants are also included. This book, therefore, brings together previously scattered information to provide an indispensable reference book for both active researchers, graduate students and anyone interested in this aspect of tissue culture technology and plant development.
Download or read book Stem Cells and the Future of Regenerative Medicine written by Institute of Medicine and published by National Academies Press. This book was released on 2002-01-25 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.
Download or read book Embryo Culture written by Gary D. Smith and published by Humana Press. This book was released on 2012-07-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.
Download or read book Human Stem Cell Manual written by Suzanne Peterson and published by Academic Press. This book was released on 2012-10-22 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America. Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.
Download or read book Comparative Embryo Culture written by Jason R. Herrick and published by Humana. This book was released on 2019-06-29 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics. Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation.
Download or read book Current Advances in Coconut Biotechnology written by C. Oropeza and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: The coconut palm occupies a significant place in the world economy as an important subsistence crop in all the areas in which it is grown. Relatively few countries are able to export any quantity of coconut products because of increasing home demands coupled with low productivity. Yields are generally well below potential despite recent developments with improved planting stock and agronomic practices. In the last 50 years, both these aspects have received considerable attention, but the focus is shifting to investigate how the use of recently developed biotechnological techniques- can benefit the coconut industry. This volume, the result of the International Symposium on Coconut Biotechnology (held in December 1997 in Merida, Yucatan, Mexico), describes recent research in three important areas. Standard plant breeding techniques used with coconut have produced improved planting material, but progress is inevitably very slow. Can more rapid genetic improvement be obtained using molecular techniques? The papers presented in this section suggest that such techniques will open up exciting new prospects, but only after basic information has been gathered on the genetic status of existing coconut stocks. Research using microsatellite techniques seems to provide a useful tool to help to classifying these stocks. However, only a combination of classical breeding methods with modem techniques will lead to the rapid improvement which is required to supply material for urgent replanting programs.
Download or read book HEW Support of Research Involving Human in Vitro Fertilization and Embryo Transfer written by United States. Department of Health, Education, and Welfare. Ethics Advisory Board and published by . This book was released on 1979 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Troubleshooting and Problem Solving in the IVF Laboratory written by Kay Elder and published by Cambridge University Press. This book was released on 2015-06-18 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Helping IVF laboratories and clinics to maintain the highest success rates possible, this is essential reading for every IVF laboratory.
Download or read book Haeckel s Embryos written by Nick Hopwood and published by University of Chicago Press. This book was released on 2015-05-11 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: Emphasizing the changes worked by circulation and copying, interpretation and debate, this book uses the case to explore how pictures succeed and fail, gain acceptance and spark controversy. It reveals how embryonic development was made a process that we can see, compare, and discuss, and how copying - usually dismissed as unoriginal
