Download or read book Electron Microscopy of Plant Pathogens written by Kurt Mendgen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Download or read book Electron Microscopy of Plant Pathogens written by Kurt Mendgen and published by Springer. This book was released on 1990-12-10 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Download or read book Plant Pathogen Detection and Disease Diagnosis written by P. Narayanasamy and published by CRC Press. This book was released on 2001-08-28 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materia
Download or read book Laboratory Exercises in Plant Pathology written by A. B. A. M. Baudoin and published by Amer Phytopathological Society. This book was released on 1988 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: How to use this kit; Use and care of the light microscope; Recognition and terminology of disease symptoms and signs; Isolation of fungi and bacteria; Diagnosis of disease and proof of pathogenicity; Plant disease diagnosis in practice; Characteristics and structures of fungi; Root infection by phytophtora; Damping-off of seeds and seedlings; Soils fungistasis; Pathogen survival in soil; Biological control of soilborne plant pathogens by antagonistic fungi; Soilborne fungi; Population assessment of fusarium spp; Foliar infection by fungi; Dissemination of fungus spores and bacterial cells by wind and water; Fungicides for the control of foliar infection; Race identification of wheat leaf rust; Bacterial pathogens; Crown gall; Frost injury and ice nucleating bacteria; Viruses: mechanical transmission and host range; Insect transmission of plant viruses; Electron microscopy; Virus identification by serology; Study of plant-parasitic nematodes; Root-Knot nematodes: Symptoms, life stages, and control; Cyst nematodes; Foliar nematodes on begonia: Physiological spects of plant disease; Abiotic diseases; Disorders caused by higher plant.
Download or read book Molecular Methods in Plant Pathology written by U. S. Singh and published by CRC Press. This book was released on 2017-12-14 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Download or read book Electron Microscopy of Plant Cells written by C Hawes and published by Academic Press. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Download or read book Electron Microscopy and Plant Ultrastructure written by Anthony William Robards and published by . This book was released on 1970 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Diagnosis of Plant Virus Diseases written by R. E. F. Matthews and published by CRC Press. This book was released on 2019-07-23 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
Download or read book Phytopathological Diagnostic Techniques written by Iftikhar Ahmad and published by . This book was released on 1992 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Principles of Diagnostic Techniques in Plant Pathology written by R. T. V. Fox and published by Oxford University Press, USA. This book was released on 1993 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: The correct diagnosis of a plant disease is an essential prerequisite of its successful control. Diagnostic methods today include a number of traditional techniques such as direct observation or microscopy, as well as more recently developed procedures such as those based on immunological or nucleic acid analysis. This book provides a text reviewing the principles of all these techniques that will be suitable for advanced students of plant pathology who already have some basic background in the subject. The theories behind the methods are described and illustrated with numerous examples of plant diseases caused by fungi, bacteria and viruses, and the strengths and limitations of different techniques are compared. The book includes a number of colour photographs and will provide a very useful overview of this rapidly developing subject.
Download or read book Techniques for Plant Electron Microscopy written by Barrie Edward Juniper and published by Blackwell Scientific Publications Limited. This book was released on 1970 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fixation. Embedding. Microtomy. General stains. Cytochemical stains. Negative staining. Specimen support films. Replicas. Preparation for scanning electron microscopy. Special techniques. Autoradiography. The instrument and photography.
Download or read book Plant Diseases Of Viral Viroid Mycoplasma And Uncertain Etiology written by Karl Maramorosch and published by CRC Press. This book was released on 2019-09-11 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: The adverse impact of plant diseases is felt around the world. In this book, the contributors analyze the nature and origins of pathogens that affect some economically important food and fiber crops.
Download or read book Plant Virology written by Aiming Wang and published by Humana. This book was released on 2022-12-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
Download or read book Newly Discovered Prokaryotic Plant Pathogens written by Eleanor Elmendorf and published by . This book was released on 1977 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Techniques in Plant Histopathology written by João Paulo Rodrigues Marques and published by Springer Nature. This book was released on 2022-10-17 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook presents a compilation of plant histopathology laboratory practices and microscopy techniques for study plant tissues under biotic stress. It will serve as an easy-to-reference material for professors, undergraduate and graduate students and researchers from different areas who work with the interaction between plants and pathogens, whether they are fungi, viruses or bacteria. Besides, it will also help unveil the structural, ultrastructural and histochemical changes induced by plants when challenged by plant pathogens.
Download or read book Histology Ultrastructure and Molecular Cytology of Plant Microorganism Interactions written by Michel Nicole and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants interact with a large number of microoganisms which have a major impact on their growth either by establishing mutually beneficial symbiotic relationships or by developing as pathogens at the expense of the plant with deleterious effects. These microorganisms differ greatly not only in their nature (viruses, phytoplasmas, bacteria, fungi, nematodes, ... ) but also in the way they contact, penetrate and invade their host. Histology and cytology have brought an essential contribution to our knowledge of these phenomena. They have told us for instance, how specialized structures of the pathogen are often involved in the adhesion and penetration into the plant, how the interface between both organisms is finely arranged at the cellular level, or what structural alterations affect the infected tissues. They have thus set the stage for the investigations of the underlying molecular mechanisms could be undertaken. Such investigations have been remarkably successful in the recent years, expanding considerably our understanding of plant-microorganism interactions in terms of biochemical changes, rapid modifications of enzymatic activities, coordinated gene activation, signal reception and transduction. Biochemistry, molecular biology and cellular physiology have taken precedence in the phytopathologist's set of methods.
