Download or read book Scanning Electron Microscopy in BIOLOGY written by R.G. Kessel and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the continuing quest to explore structure and to relate struc tural organization to functional significance, the scientist has developed a vast array of microscopes. The scanning electron microscope (SEM) represents a recent and important advance in the development of useful tools for investigating the structural organization of matter. Recent progress in both technology and methodology has resulted in numerous biological publications in which the SEM has been utilized exclusively or in connection with other types of microscopes to reveal surface as well as intracellular details in plant and animal tissues and organs. Because of the resolution and depth of focus presented in the SEM photograph when compared, for example, with that in the light microscope photographs, images recorded with the SEM have widely circulated in newspapers, periodicals and scientific journals in recent times. Considering the utility and present status of scanning electron microscopy, it seemed to us to be a particularly appropriate time to assemble a text-atlas dealing with biological applications of scanning electron microscopy so that such information might be presented to the student and to others not yet familiar with its capabilities in teaching and research. The major goal of this book, therefore, has been to assemble material that would be useful to those students beginning their study of botany or zoo logy, as well as to beginning medical students and students in advanced biology courses.
Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Download or read book Advanced Techniques in Biological Electron Microscopy II written by J. K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of the term "advanced" in the title of this book is somewhat ar bitrary and very much relative with respect to time. Many techniques which were considered at the "cutting edge" of ultrastructural methodology just a few years ago are now rou tin ely used in numerous laboratories. One could cite freeze-fracture, cryothin sectioning, or indeed most of the field of scan ning electron microscopy as concrete examples. Thus the use of the term "ad vanced techniques" must be interpreted with regard to the present state of the art, and is useful only in informing the potential reader that this volume is not a primer to be used as an initial introduction into basic biological elec tron microscopy. Many excellent volumes have filled that niche in the past few years, and it is not intended that this modest book be a complete com pendium of the field. Furthermore, any limited selection of papers on advanc ed techniques necessarily reflects the preferences and arbitrary whims of the editor, thereby excluding many equally important procedures which the knowledgeable reader will readily identify. The first volume of this series appeared approximately five years ago and illustrated techniques which were thought to represent advanced and yet ba sically morphological methods for gaining increased ultrastructural informa tion from biological specimens. The present volume, on the other hand, stresses techniques which provide specific physicochemical data on the speci mens in addition to the structural information.
Download or read book Biological Electron Microscopy written by Michael J. Dykstra and published by Springer Science & Business Media. This book was released on 2003-12-31 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
Download or read book The Principles and Practice of Electron Microscopy written by Ian M. Watt and published by Cambridge University Press. This book was released on 1997-01-30 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of this book was widely praised as an excellent introduction to electron microscopy for materials scientists, physicists, earth and biological scientists. This completely revised new edition contains expanded coverage of existing topics and much new material. The author presents the subject of electron microscopy in a readable way, open both to those inexperienced in the technique, and also to practising electron microscopists. The coverage has been brought completely up to date, whilst retaining descriptions of early classic techniques. Currently live topics such as computer control of microscopes, energy-filtered imaging, cryo- and environmental microscopy, digital imaging, and high resolution scanning and transmission microscopy are all described. The highly praised case studies of the first edition have been expanded to include some interesting new examples. This indispensable guide to electron microscopy, written by an author with thirty years practical experience, will be invaluable to new and experienced electron microscopists in any area of science and technology.
Download or read book Advances in Imaging and Electron Physics written by and published by Academic Press. This book was released on 2009-11-05 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Imaging and Electron Physics merges two long-running serials--Advances in Electronics and Electron Physics and Advances in Optical and Electron Microscopy. This series features extended articles on the physics of electron devices (especially semiconductor devices), particle optics at high and low energies, microlithography, image science and digital image processing, electromagnetic wave propagation, electron microscopy, and the computing methods used in all these domains. This particular volume presents several timely articles on the scanning transmission electron microscope. - Updated with contributions from leading international scholars and industry experts - Discusses hot topic areas and presents current and future research trends - Provides an invaluable reference and guide for physicists, engineers and mathematicians
Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Download or read book Principles and Techniques of Scanning Electron Microscopy written by M. A. Hayat and published by . This book was released on 1974 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Beginner s Handbook in Biological Transmission Electron Microscopy written by Brenda S. Weakley and published by . This book was released on 1981 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Simplified basic theory of transmission electron microscopy; The processing of tissues; The preparation of ultrathin sections for electron microscopy; Staining of biological materials for electron microscopy; Routine operation of the electron microscope; Darkroom procedures; Some general suggestions for the study of ultrastructure; Ultrastructural cytochemistry; Autoradiography with the electron microscope; A brief synopsis of other techniques for the electron microscope; Routine maintenance of the electron microscope.
Download or read book Biology of the Uterus written by Ralph Wynn and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the decade following the publication of the first edition of Cellular Biology of the Uterus, advances in this field have been so rapid as to require not merely a revision of the earlier text but an essentially new volume. Even the title of the book has been changed, to Biology of the Uterus, to reflect the incorporation of more material based on classical anatomy and physiology. This histological and embryological information provides a necessary, though often lacking, background for the protein chemist and molecular biologist, and a bridge between biochemistry and biophysics, on the one hand, and clinical medicine, on the other. Thus, major practical problems in human reproduction, such as the mode of action of contraceptive agents and the cause of the initiation of labor, may be approached on a firm scientific footing. This text deals primarily with the biology of the uterus itself (comparative and human) rather than with placentation or pregnancy, and as such is a synthesis of data derived from many techniques, conventional and modern. Inasmuch as it is clearly beyond the competence of anyone scientist to prepare such a text on the basis of personal knowledge and experience, the aid of distinguished biologists from this country and abroad was enlisted. All of these authors, acknowledged experts in their respective fields, agreed to extensive revision of their chapters or preparation of entirely new contributions.
Download or read book National Library of Medicine Current Catalog written by National Library of Medicine (U.S.) and published by . This book was released on 1971 with total page 1324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nuclear Science Abstracts written by and published by . This book was released on 1975 with total page 1070 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Correlative Microscopy In Biology written by M.A. (Eric) Hayat and published by Elsevier. This book was released on 2012-12-02 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: Correlative Microscopy in Biology: Instrumentation and Methods presents the detailed methodology of biological correlative microscopy, a technology that allows the acquisition of multiple data from single tissue block, cell, or section. The chapters in the book include detailed and complete instructions on the preparatory procedures. The book has 20 chapters that deal with various forms and systems of microscopy. Some of the forms and methods used in the book include light, scanning electron, fluorescence, scanning transmission electron, and ion microscopy, as well as combined light and electron and transmission electron microscope. Other methods and their applications are all discussed in detail in the book. This book will help students apply the methods without outside help as each methodology is presented in a step-by-step approach, including applications and techniques. Aside from students, the book will also be good reference for teachers, scientists, and researchers in the fields of biology, biochemistry, and medicine.
Download or read book Hagfish Biology written by Susan L. Edwards and published by CRC Press. This book was released on 2015-09-14 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: With over 70 species still populating the world's oceans after approximately 500 million years, hagfishes are essential benthic organisms that play a vital role in understanding the evolutionary origins of vertebrate life and the maintenance of the oceanic ecosystem. Hagfish Biology is a long overdue book for communicating and furthering study on t
Download or read book Cell Biology of Physarum and Didymium V2 written by Henery Aldrich and published by Elsevier. This book was released on 2012-12-02 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Biology of Physarum and Didymium, Volume II: Differentiation, Metabolism, and Methodology summarizes important experimental research using Physarum and Didymium for developmental and cellular studies. This book is organized into three parts, encompassing 28 chapters that cover the differentiation, metabolism, and experimental techniques for cellular studies. After presenting an introduction to some aspects of developmental biology, this volume describes the ultrastructure and physiology of sporulation, spore germination, encystment, excystment, spherulation, and spherule germination. This is followed by a discussion on regulatory events leading to morphogenesis and on biochemical, physiological, and structural data on the amoeboid stage. The second part focuses on metabolic aspects. This includes metabolic characteristics of myxomycetes; the formation of nucleotides in Physarum by de novo synthesis and from nucleic acid degradation products; and radiation and radiomimetic agents on myxomycete species. Considerable chapters in the concluding part are devoted to procedures and protocol for isolation of cell components from Physarum and Didymium species. This volume also evaluates some techniques, including electron microscopy, time-lapse microcinematography, phase-contrast microscopy, Feulgen staining, and culture methods. The concluding chapters examine the preparation, isolation, and characterization of ribonucleic acid, histone, plasmodial polysaccharides, myosin, actins, and fragmin. The book will serve as a frequent, single reference source to brief cell biologists on the primary research on Physarum and Didymium. It will also be a good source for graduate students in cell biology and perhaps in other graduate courses.
Download or read book Fixation for Electron Microscopy written by M.A. (Eric) Hayat and published by Elsevier. This book was released on 2012-12-02 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fixation for Electron Microscopy presents how to better understand the effects of fixatives on the molecular structure of the cell. This book attempts to consider each aspect of fixation, including chemical interactions between fixatives and individual cellular substances. The chemistry of fixative interactions that are discussed in the book is based primarily on the reactions of a fixative with isolated proteins, lipids, nucleic acids, and carbohydrates. The book shows that the correct interpretation of information retrieved from electron micrographs depends on the knowledge of the basic principles underlying the fixation procedure. Also, the book presents the fixation of both eukaryotic and prokaryotic specimens. The special fixation conditions for plant specimens are discussed in detail and have been allotted a whole chapter. Also emphasized in this book is the connection between morphology and biochemical aspects of preparatory treatments and the chemical basis of the formation of artifacts. This topic is useful in understanding the modifications of cell structures introduced during their processing. A guide for recognizing and minimizing major artifacts and fixation faults that are usually encountered is also presented in the book. This valuable resource will prove useful to both students and professionals in the field of biology and clinical medicine. Specimen preservation researchers can also benefit from this book.
Download or read book Biology of the Ovary written by P. Motta and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensive reviews have been published on the mor aspects, anatomy, ultrastructure, physiology, bio phology, anatomy, and physiology of the mam chemistry, endocrinology, pharmacology, and physiopathology of the mammalian ovary. The malian ovary. However, little attention has been given to the gaps between the physiological, endo contributors, who prepared their chapters metic ulously, are recognized experts in their respective crine and histochemical parameters of the ovary as disciplines and their writings reflect extensive per it relates to clinical aspects. There is a wealth of sonal experience and unpublished data of both publications concerning the biology of the ovary: basic and clinical sciences. Without the assistance of the purpose of this volume is to integrate this information with emphasis on modern concepts in good friends and permission to draw extensively microanatomy, physiology, methodology, clinical from investigations, this volume would not have complications, and therapeutic approaches. An been possible. attempt is made to place fundamental research in Thanks are due to G. F. Franchitto, F. Barberini, clear perspectives. P. Stoops. Lori Rust and J. Squiers for their editorial It is hoped that this volume will fulfil a long skills and for checking the references to the original standing need and serve as an important source for sources. Thanks are particularly due to 1. K. Smith investigators and clinicians concerned with the of Martinus Nijhoff Publishers BV for his excellent physiopathology of the ovaries for years to come. It cooperation during the production of the book.
