Download or read book Dioxygen Binding and Sensing Proteins written by Martino Bolognesi and published by Springer Science & Business Media. This book was released on 2008-12-16 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following their own brilliant careers in haeme protein research, the outstanding scientists Jonathan and Beatrice Wittenberg continue to provide inspiration to the research community in the study of oxygen-binding proteins. Their research has provided the intellectual stimulus to bring together scientists from all over the world with the common goal of developing fascinating new ideas and performing innovative experiments. This book is dedicated to Jonathan’s and Bea’s lifetime careers. It further illuminates the facts and ideas which dot the paths they traced in Biochemistry and Physiology, elaborating on how these landmark achievements were made and how the haeme proteins community still refers to them. With the field of haeme protein science such a flourishing area, the contributors to this book predict Jonathan and Bea, having played such a seminal role, will continue to be key figures for quite some time to come.

Download or read book XVIth International Conference on Dioxygen Binding and Sensing Proteins O2BIP written by Sylvia Dewilde and published by . This book was released on 2011 with total page 85 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Oxygen Binding and Sensing Proteins written by Andrea Mozzarelli and published by . This book was released on 2013 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dioxygen dependent Heme Enzymes written by Masao Ikeda-Saito and published by Royal Society of Chemistry. This book was released on 2018-10-01 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the many and varied catalytic activities of O2-dependent heme-iron enzymes, including monoxygenases and cytochrome P450, dioxygenases, oxidases and model heme systems required for postgraduate students and researchers in biochemistry and metallobiology.

Download or read book Devoted to the XIVth International Conference on Dioxygen Binding and Sensing Proteins Naples Italy 3 7 September 2006 written by Luc Moens and published by . This book was released on 2007 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Special Issue written by Luc Moens and published by . This book was released on 2007 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Sensors and Reactive Oxygen Species written by Helmut Sies and published by Academic Press. This book was released on 2002 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is a companion to Volume 347 and addresses direct sensing of reactive oxygen species and related free radicals by thiol enzymes and proteins.

Download or read book Special Issue Devoted to the XIVth International Conference on Dioxygen Binding and Sensing Proteins written by and published by . This book was released on 2007 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book XVth International Conference on Oxygen Binding and Sensing Proteins O2BiP written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Sensors and Reactive Oxygen Species Part B Thiol Enzymes and Proteins written by and published by Elsevier. This book was released on 2002-03-06 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is a companion to Volume 347 and addresses direct sensing of reactive oxygen species and related free radicals by thiol enzymes and proteins.

Download or read book Tuning Sensing and Signaling in the H NOX Family of Heme Proteins written by Michael B. Winter and published by . This book was released on 2011 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitric oxide (NO) signaling in mammals occurs through activation of the soluble isoform of guanylate cyclase (sGC), which results in diverse physiological processes such as blood vessel dilation and neurotransmission. sGC is a heterodimeric heme protein that has evolved to be a specific sensor for NO. Although sGC contains the same histidyl-ligated porphyrin as the globins, it has no measureable affinity for oxygen and does not oxidize in air. These unusual features facilitate selective and robust activation by NO in aerobic cellular contexts. Questions regarding the mechanism of ligand discrimination in sGC led to the identification of sGC heme domain homologues in many different organisms where these proteins function as NO and/or oxygen sensors for gas-mediated signaling pathways. The members of the protein family have been subsequently termed Heme Nitric oxide/OXygen binding (H-NOX) domains due to their divergent ligand-binding properties. Functional characterization of H-NOX domains from prokaryotes has provided important clues about the structural features that control ligand discrimination across the H-NOX family. However, additional fundamental questions remain about the influence of protein structure on heme chemistry. Extensive studies on the globins, as model heme proteins, have established the functional importance of heme protein topological features in modulating gas diffusion to and from the heme site. In globins, deep gas pockets around the porphyrin transiently capture and release oxygen, tuning oxygen affinity for transport, delivery, and storage in diverse physiological environments. Although mechanisms of gas binding in H-NOX proteins have been a subject of intense investigation, there is little knowledge regarding the functional role of protein structure in modulating gas diffusion. Using X-ray crystallography with xenon and kinetic measurements, a tunnel network that extends between the solvent and interior heme site was mapped in a prokaryotic H-NOX domain. Hindering gas diffusion through the tunnels has important consequences on diatomic gas affinity. This suggests that protein tunnels in H-NOX proteins may play functional roles in tuning gas-mediated signaling. Unlike isolated H-NOX domains, mammalian sGC is a structurally more complex, multi-domain protein. The ability of the sGC heme to resist oxidation in air is unique among histidyl-ligated heme proteins and essential for maximal NO-induced activation. Speculations have been made about how the sGC heme resists oxidation, but no study has systemically addressed the structural and electronic factors that contribute to this critical property. To probe the accessibility of oxygen to the heme site, we sought to substitute the native heme of sGC with an unnatural porphyrin displaying emission that is quenched by oxygen. Using an expression-based methodology, a phosphorescent Ru porphyrin was incorporated in sGC constructs of varying lengths. Emission quenching results suggest that oxygen diffusion to the full-length sGC heme site is significantly hindered compared to smaller sGC constructs. Limited oxygen accessibility, combined with heme electronic factors, appear to serve as important evolutionary solutions in sGC to protect the heme cofactor under aerobic conditions. Originally to probe sGC function, the strategies used to generate oxygen-sensing proteins have been applied to create a new family of H-NOX-based molecular sensors. Substitution of the native heme in bacterial H-NOX proteins with unnatural porphyrins has proven to be a promising strategy to develop stable protein agents for biological imaging applications. Heme proteins are highly tunable frameworks that coordinate porphyrins with high fidelity and specificity. These properties have been exploited to design heme protein-based sensors with tailored functionalities for enhancing porphyrin bioavailability. Initial work on making optical oxygen sensors has evolved into recent efforts to generate H-NOX-based MRI contrast agents with high relaxivities for future use in deep-tissue imaging. Thus, through substitution of the native heme group with unnatural porphyrins, new sensing properties have been built into the H-NOX family.

Download or read book Oxygen Sensing written by Emily E. Weinert and published by Springer Nature. This book was released on 2023-04-11 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides researchers with protocols that help them investigate known or putative O2 sensing proteins and pathways. The chapters in this book discuss techniques ranging from anaerobic redox midpoint measurement to approaches to control expression of globin genes, which provide detailed methods for researchers interested in expanding their knowledge of O2 sensing systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Oxygen Sensing: Methods and Protocols is a valuable resource to a wide audience, ranging from microbiologists and cell biologists, to protein biochemists.

Download or read book Oxygen Sensing and Signal Transduction by the Rhizobium Meliloti Oxygen Sensor Protein FixL written by Ellen Kristine Monson and published by . This book was released on 1994 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Hemoglobin written by Richard Earl Dickerson and published by Benjamin-Cummings Publishing Company. This book was released on 1983 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Gas Sensing in Cells written by Shigetoshi Aono and published by Royal Society of Chemistry. This book was released on 2017-11-07 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the first comprehensive overview of gas sensing in living cells and describes a wide spectrum of proteins that produce, sense or use gas molecules in both prokaryotic and eukaryotic cells.

Download or read book Nitric Oxide Sensing written by Sagarika Bhattacharya and published by CRC Press. This book was released on 2021-09-16 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although nitric oxide (NO) is an important biological signaling molecule, its free-radical electronic configuration makes it a most reactive molecule and the scariest colorless gas causing immense environmental and health hazards. Detection of NO levels in biological samples and in the atmosphere is therefore crucial. In the past few years, extensive efforts have been devoted to developing many active sensors and effective devices for detecting and quantifying atmospheric NO, NO generated in biological samples, and NO exhaled in the human breath. This book provides a concrete summary of recent state-of-the-art small-molecule probes and novel carbon nanomaterials used for chemical, photoluminescent, and electrochemical NO detection. One chapter is especially dedicated to the available devices used for detecting NO in the human breath that indirectly infers to lung inflammation. The authors with expertise in diverse dimensions have attempted to cover almost all areas of NO sensing.

Download or read book Oxygen Sensing written by and published by Elsevier. This book was released on 2004-05-10 with total page 867 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields.