Download or read book Development of New Liquid Chromatography Techniques for Mass Spectrometric Analysis of Metabolites and Proteins written by Zhihui Wen and published by . This book was released on 2006 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Liquid Chromatography Mass Spectrometry written by Alfred L. Yergey and published by Springer Science & Business Media. This book was released on 1990-01-31 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended both to be an introduction to techniques and applications of liquid chromatography/mass spectrometry and to serve as a reference for future workers. When we undertook its writing, we chose not to cover the field, particularly applications, exhaustively. Rather we wished to produce a book that would be of use to people just beginning to use the technique as well as to more advanced practitioners. In this regard, we have sought to highlight techniques and applications that are of current importance, while not neglecting descriptions of approaches that may be of significance in the future. We hope that we have succeeded in this. At the same time we hope that the bibliography, with indexes classified by author and title, will make this book of value to those who may disagree with our emphasis. ACKNOWLEDGMENTS. One of us (C. G. E. ) wishes to acknowledge the en couragment of Professor J. A. McCloskey in undertaking this project. All four of us are grateful for the continuous and expert assistance of V. A. Edmonds in the preparation of the Bibliography. Alfred L. Y ergey Bethesda, Maryland Charles G. Edmonds Richland, Washington lvor A. S. Lewis London, England Marvin L. Vestal Houston, Texas v Contents 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . 1 . . . . . . . . . . . 2. Direct Liquid Introduction Interfaces . . . . . . . . . . 5 . . . 2. 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 2. Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. 3. Specific DLI Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 1. Capillary Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 2. Diaphragm Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2. 3. 3. Nebulizing Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Download or read book Ultra Performance Liquid Chromatography Mass Spectrometry written by Mu Naushad and published by CRC Press. This book was released on 2014-03-18 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to its high sensitivity and selectivity, liquid chromatography–mass spectrometry (LC–MS) is a powerful technique. It is used for various applications, often involving the detection and identification of chemicals in a complex mixture. Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis presents a unique collection of up-to-date UPLC-MS/MS methods for the separation and quantitative determination of components, contaminants, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. The book begins with an overview of the history, principles, and advancement of chromatography. It discusses the use of UHPLC techniques in food metablomics, approaches for analysis of foodborne carcinogens, and details of UPLC-MS techniques used for the separation and determination of capsaicinoids. Chapters describe the analysis of contaminants in food, including pesticides, aflatoxin, perfluorochemicals, and acrylamide, as well as potentially carcinogenic heterocyclic amines in cooked foods. The book covers food analysis for beneficial compounds, such as the determination of folate, vitamin content analysis, applications for avocado metabolite studies, virgin olive oil component analysis, lactose determination in milk, and analysis of minor components of cocoa and phenolic compounds in fruits and vegetables. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.

Download or read book Liquid Chromatography Mass Spectrometry written by WILFRIED M.A. NIESSEN and published by CRC Press. This book was released on 2020-06-30 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offering well-rounded coverage of the most significant technological developments in the past few years, this classic work explains how to use LC-MS technology and interpret LC-MS data for various disciplines. This edition places a stronger emphasis on applications, dedicating more than two-thirds of the text to examining LC-MS in emerging areas

Download or read book Liquid Chromatography written by Wilfried M.A. Niessen and published by CRC Press. This book was released on 1998-09-01 with total page 654 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume comprehensively relates developments, principles, and applications of combined liquid chromatography-mass spectrometry and other techniques such as capillary electrophoresis and supercritical fluid chromatography combined with mass spectrometry. It covers historical developments, currently important interfaces and technologies, and LC-MS applications in environmental analysis, pharmaceuticals and bioanalysis, and additional fields. It offers in-depth coverage of interfaces and technologies currently important in the laboratory, especially electrospray and APCI, contains an expanded applications section, and provides over 2200 references, tables, equations, and drawings.

Download or read book Liquid Chromatography Mass Spectrometry written by Robert E. Ardrey and published by John Wiley & Sons. This book was released on 2003-07-25 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: First explaining the basic principles of liquid chromatography and mass spectrometry and then discussing the current applications and practical benefits of LC-MS, along with descriptions of the basic instrumentation, this title will prove to be the indispensable reference source for everyone wishing to use this increasingly important tandem technique. * First book to concentrate on principles of LC-MS * Explains principles of mass spectrometry and chromatography before moving on to LC-MS * Describes instrumental aspects of LC-MS * Discusses current applications of LC-MS and shows benefits of using this technique in practice

Download or read book Advances in Mass Spectrometry written by Gareth A.G. Brenton and published by Elsevier. This book was released on 2004-06-04 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains contributions based on many of the Invited Lectures given at the 16th triennial International Mass Spectrometry Conference (IMSC) in Edinburgh (September 2003) while the accompanying CD contains the Abstracts of all the accepted papers-Oral and Posters. Traditionally the IMSC has a strong emphasis on the fundamentals and new instrumentation of mass spectrometry and on instrumental developments, and this tradition has continued. There was, however, an equally strong emphasis in Edinburgh on key application areas in mass spectrometry like genomics, proteomics, metabonomics; drug metabolism and bioanalysis, discovery pharma, analysis in pharmaceutical development processes; environmental chemistry, food and nutrition, forensics, and engineering materials. The Invited Lecturers are all international experts. Their articles not only summarise the current state of mass spectrometry in their own areas of expertise but also highlight the current hot topics in mass spectrometry. A must-have for all libraries as an up-to-date summary. - Presents a current summary of mass spectrometry. - Covers both main mass spectrometric applications and developments. - International group of contributors.

Download or read book Sample Preparation in LC MS Bioanalysis written by Wenkui Li and published by John Wiley & Sons. This book was released on 2019-02-25 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis Following in the footsteps of the previously published Handbook of LC-MS Bioanalysis, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography–Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare. While advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include: Current basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes) Sample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor) Crucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates) Sample Preparation in LC-MS Bioanalysis will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring.

Download or read book Mass Spectrometry written by Ganesh Shamrao Kamble and published by BoD – Books on Demand. This book was released on 2019-11-13 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of the emerging techniques and applications of mass spectrophotometry in various fields of sustainable chemistry. At the current level of development, the book provides "real-life" examples of analyses and applications. All the chapters are written by internationally recognized authors, and present qualitative and quantitative applications of mass spectrometry. These multidisciplined chapters cover the fundamentals as well as recent advanced analytical techniques, and offer significant information for various researchers studying biomedical, pharmaceutical, and environmental analysis.

Download or read book Liquid Chromatography written by G. Baird and published by Elsevier Inc. Chapters. This book was released on 2013-01-08 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical chemistry is the discipline within laboratory medicine concerned with measuring the concentrations of analytes in blood, body fluids, and tissues. Liquid chromatography is already commonly used in the clinical chemistry laboratory for measuring the concentrations of small molecules, such as drugs and hormones, using a variety of solid phases for separation and numerous detection modalities, most important mass spectrometry. Additional liquid chromatographic methods are now being developed for measuring the concentrations of proteins in blood and tissues, taking advantage of the rapid development of novel mass-spectrometry instruments and methodologies.

Download or read book Liquid Chromatography and Mass Spectrometry Based Analytical Method Development Towards Fast and Sensitive Analysis written by Juan Bian and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid chromatography (LC) and mass spectrometry (MS) is a valuable tool in pharmaceutical analysis and is widely applied to the analysis of many real-world samples. Recent achievements in material and instrumentation have allowed LC and MS to play a critical role in the bioanalytical application. However, when dealing with a sample with high complexity, such as metabolites or proteins from biological samples, current LC and MS-based methods may not provide sufficient analytical results. As a consequence, this dissertation focuses on new LC and MS-based method development to address the challenges in small molecules and macromolecules analysis.

Download or read book Hydrophilic Interaction Chromatography written by Bernard A. Olsen and published by John Wiley & Sons. This book was released on 2013-01-22 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how to use HILIC to analyze and better understand polar compounds An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications. Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring: Detailed examples of HILIC methods and development approaches Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods References to the primary literature at the end of each chapter Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.

Download or read book High Resolution Mass Spectrometry and Its Diverse Applications written by Sreeraj Gopi and published by CRC Press. This book was released on 2022-12-22 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This informative book offers a wide range of knowledge on the technologies and applications of the cutting-edge field of high-resolution mass spectrometry (HRMS) in different areas of analysis. HRMS has changed the nature of experimentation and investigation in so many analytical realms. Determining exact mass determination, high resolution, and specificity—via the special features provided by HRMS instruments—is now possible for determining the composition of the analyte of interest, both qualitatively and quantitatively. High-Resolution Mass Spectrometry and Its Diverse Applications: Cutting-Edge Techniques and Instrumentation begins with an overview of the basic instrumentation techniques and goes on to present research on diverse new uses of HRMS in clinical testing, such as for therapeutic drug designing, discovery, and development; in forensic studies and investigations; in quality management systems; for analysis of pesticides; for analysis of single cells; in analysis of fossil fuels; for use in space and planetary science; and more. Chapters relay how HRMS plays an important role in the structure elucidation and unknown determination in many fields and is a great measure to be used for quantitative analyses. The book considers how these properties make the technique a strong aid in many areas. This volume highlights how HRMS can be a useful tool for scientists and researchers, faculty and students, and industry professionals in many scientific areas of study.

Download or read book Supercritical Fluid Chromatography And Micro hplc written by M. Yoshioka and published by VSP. This book was released on 1989 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1988. Routledge is an imprint of Taylor & Francis, an informa company.

Download or read book Using Mass Spectrometry for Drug Metabolism Studies written by Walter A. Korfmacher and published by CRC Press. This book was released on 2009-11-03 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of this now classic work helped to establish mass spectrometry as the premier tool for drug metabolism studies. Completely rewritten from start to finish, Using Mass Spectrometry for Drug Metabolism Studies, Second Edition brings medicinal chemists and mass spectrometry professionals up to speed with the rapid advances in the fiel

Download or read book Development of Liquid Chromatography mass Spectrometric Assays and Sample Preparation Methods for the Biological Sample Analysis written by Sujatha Chilakala and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The area of biosample analysis encompasses a very broad range of assays which support the clinical and nonclinical studies. Biosample analysis is used to provide a quantitative or qualitative measure of the active drug and/or its metabolite(s) in the biological matrix for the purpose of pharmacokinetics, toxicokinetics, bioequivalence, and exposure-response (pharmacokinetics /pharmacodynamics) studies. Due to the significance of pharmacological analysis, sensitive, reproducible and robust analytical methods are critically needed for pharmacological studies of the biosamples. A bioanalytical method mainly contains two components I) Sample preparation II) detection of the compound. Therefore, the main aims of this thesis are the development of quantitative and qualitative analytical methods for the target compounds using LC-MS(/MS) and development of accelerated sample preparation for high throughput sample analysis for DNA and proteins.In this dissertation, a brief review on the method rationale, workflow of the method development, sample preparation methods, instrumentations and analytical method validation, are discussed in Chapter 1. Also, research projects were discussed and the techniques used in the experiments for this thesis were reviewed. As so, chapter II and III were mainly focused on the accelerated sample preparation methods for the high throughput sample analysis of DNA and proteins respectively, where the sample preparation time was significantly reduced from hours to minutes, which are suitable for qualitative and quantitative analysis of DNA and proteins. In Chapter IV, a systematic study on the structural characterization of the model glycoprotein Human IgG was described. In chapter V successful development of LC-MS method was developed for the determination of Oxygen -18 isotope enrichment in the phosphate samples in the positional isotope exchange reactions to study the reversibility of certain enzymatic reactions was described. Successful development and validation of a new and sensitive analytical LC-MS/MS method for the determination and quantitation of incorporation rates of decitabine, an anti-cancer drug which can be applied to determine the sensitivity and responsiveness in patients treated with decitabine was described in Chapter VI.

Download or read book Handbook of Advanced Chromatography Mass Spectrometry Techniques written by Michal Holcapek and published by Elsevier. This book was released on 2017-09-07 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques. Includes UHPLC, HILIC, nano-liquid chromatographic separations, two-dimensional LC-MS (LCxLC), multiple parallel MS, 2D-GC (GCxGC) methodologies for lipids analysis, and more Contains both practical and theoretical knowledge, providing core understanding for implementing modern chromatographic and mass spectrometric techniques Presents chapters on the most popular and fastest-growing new techniques being implemented in diverse areas of research