Download or read book Development of Methodology for the Study of Nucleic Acids from Single Cells Using Capillary Electrophoresis written by Jennifer Lee Zabzdyr and published by . This book was released on 2004 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Humana. This book was released on 2011-12-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single–strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Download or read book Development of New Analytical Method for Specific Nucleic Acid Sequences Using Affinity Capillary Electrophoresis written by 足立賢 and published by . This book was released on 2009 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleic Acid Electrophoresis written by Dietmar Tietz and published by Boom Koninklijke Uitgevers. This book was released on 1998-10-22 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Download or read book Capillary Electrophoresis of Nucleic Acids written by and published by Springer. This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleic Acid Electrophoresis written by Dietmar Tietz and published by . This book was released on 1998-10-22 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA).
Download or read book Capillary Electrophoresis based Methodology Development for Biomolecule Analysis written by NI. Li and published by . This book was released on 2011 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) is a separation tool with wide applications in biomolecule analysis. Fast and high-resolution separation requiring minute sample volumes is advantageous to study multiple components in biological samples. Flexible modes and methods can be developed. In this thesis, I focus on developing and applying novel CE methods to study multi-target nucleic acid sensing with high sensitivity (Part I) and interactions between multiple components, i.e. proteins, nanoparticles and drugs (Part II).
Download or read book Prenatal Diagnosis written by Brynn Levy and published by Humana. This book was released on 2018-12-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the first edition with more detailed methodologies on prenatal testing and diagnosis, and also covers next-generation sequencing techniques. The chapters in this book are divided into three sections: preimplantation genetic testing, traditional prenatal testing, and non-invasive prenatal testing. This book covers topics such as molecular testing for preimplantation genetic diagnosis of single gene disorders; DNA extraction from various types of prenatal specimens; prenatal diagnosis of cystic fibrosis and Tay-Sachs disease; chromosomal SNP microarrays; and isolation of cell-free DNA from maternal plasma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Prenatal Diagnosis, Second Edition is a valuable resource for any researcher interested in reproducing these techniques in their clinical laboratories.
Download or read book Capillary Electrophoresis written by Paul D. Grossman and published by Academic Press. This book was released on 2012-12-02 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations. This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2004 with total page 788 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidics for Single Cell Analysis written by Jin-Ming Lin and published by Springer Nature. This book was released on 2019-08-28 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Download or read book Clinical Applications of Capillary Electrophoresis written by Terry M. Phillips and published by Humana Press. This book was released on 2012-09-13 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1666 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Handbook for DNA Encoded Chemistry written by Robert A. Goodnow, Jr. and published by John Wiley & Sons. This book was released on 2014-04-28 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively describes the development and practice of DNA-encoded library synthesis technology. Together, the chapters detail an approach to drug discovery that offers an attractive addition to the portfolio of existing hit generation technologies such as high-throughput screening, structure-based drug discovery and fragment-based screening. The book: Provides a valuable guide for understanding and applying DNA-encoded combinatorial chemistry Helps chemists generate and screen novel chemical libraries of large size and quality Bridges interdisciplinary areas of DNA-encoded combinatorial chemistry – synthetic and analytical chemistry, molecular biology, informatics, and biochemistry Shows medicinal and pharmaceutical chemists how to efficiently broaden available “chemical space” for drug discovery Provides expert and up-to-date summary of reported literature for DNA-encoded and DNA-directed chemistry technology and methods
