Download or read book Development and Validation of Novel Methods for Microbial Source Tracking Based on Escherichia Coli as an Indicator of Water Quality written by Maxim Sheludchenko and published by . This book was released on 2011 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Source Tracking written by Jorge W. Santo Domingo and published by Emerging Issues in Food Safety. This book was released on 2007 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a state-of-the-art review of the current technology and applications being utilized to identify sources of fecal contamination in waterways. - Serves as a useful reference for researchers in the food industry, especially scientists investigating etiological agents responsible for food contamination. - Provides background information on MST methods and the assumptions and limitations associated with their use. - Covers a broad range of topics related to MST, including environmental monitoring, public health and national security, population biology, and microbial ecology. - Offers valuable insights into future research directions and technology developments.
Download or read book Microbial Source Tracking written by Michèle Gourmelon and published by Frontiers Media SA. This book was released on 2022-01-18 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Evaluation of Different Methods for Detection and Enumeration of Indicator Organisms in Water Bodies of Northern New Jersey written by Alexandra Peri and published by . This book was released on 2013 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Source Tracking and Survival of Escherichia Coli written by Cynthia L. Meays and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantitative Microbial Source Tracking Using Bacteroidales as Fecal Source Identifier written by Dan Wang and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Fecal pollution is a primary concern for recreational water bodies in US. The current water quality criteria are based on the cultivability of fecal indicator bacteria like E. coli and enterococci. These indicators are ubiquitous in the digestive tract of warm-blooded animals but are also known to persist and even multiply in the environment given favorable conditions. Hence their presence does not provide information on the sources of fecal contamination. In the past decade microbial source tracking (MST), the research area that uses host-specific microbes to identify the sources of fecal pollution, has undergone rapid development. A brief historical overview is provided in Chapter 1 and state-of-the art methods are presented and analyzed in Chapter 2. Quantitative PCR (qPCR)-based MST methods have been widely used for two reasons: they can detect both cultivable and uncultivable microbes and their process time is shorter comparing to other MST methods. The results from qPCR reactions need to be further processed to achieve quantitative MST because of analytical complications caused by the environmental water matrix. Two of the most important aspects will be addressed in Chapters 3 and 4. Chapter 3 attempts to solve the imperfect specificity and sensitivity of the qPCR assays with a statistical procedure that evaluates the impact of false positive/negative information. Chapter 4 addresses the inaccuracy in qPCR data analysis caused by the high noise signal and inconsistent amplification efficiency frequently seen in environmental water samples. Corrective measures are proposed to improve the accuracy of estimated DNA concentrations.
Download or read book Handbook of Online and Near real time Methods in Microbiology written by Maximilian Lackner and published by CRC Press. This book was released on 2017-09-18 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid detection and indication of the microbiological quality of liquids is an emerging topic that has high potential for numerous applications in the fields of environmental monitoring, industrial process control and medical surveillance. Latest technologies allow online and near-real-time quantitative or qualitative microbial measurements with a significantly higher temporal resolution than traditional methods. Such novel developments will significantly enhance quality monitoring of water resources and liquids and have great capability for automation, control and optimization of industrial processes. Therefore, such methods are assumed to have major impacts on scientific research and technical applications in the near future. The book presents cutting edge research on frontiers in microbiological detection from leading experts: Seven chapters containing review articles on emerging and state-of-the-art online and near-real-time methods of microorganism detection and – indication are giving a comprehensive insight into this novel field. A balance between chapters from industry and contributions from academia was aimed for, covering the broad field of microbiological quality of waters and liquids in environmental, industrial and medical systems. This handbook also contains an extensive glossary pointing out and describing relevant terms and definitions. This handbook is the first of its kind and is a timely, comprehensive source of information for researchers and engineers in the areas of biotechnology, environmental sciences, control technology and the process industries.
Download or read book Bacterial Source Tracking and Survival of Escherichia Coli written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface water is used for drinking by many people around the world. E. coli is the most frequently used bacterial indicator used for assessing water quality. The survival, sources, and concentrations of E. coli were examined through a series of experiments that investigated the survival of beef cattle E. coli on land and in water, and used bacterial source tracking (BST) to determine the sources of fecal contamination diurnally and annually in multiple watersheds in British Columbia. A fecal pat experiment was conducted to examine the survival of E. coli under 4 levels of solar exposure. E. coli survived longer with increasing shade. Age of fecal pats, as well as exposure to solar radiation negatively influenced the survival of E. coli. The survival of E. coli in stream water was examined in filtered and unfiltered stream water at 3 different temperatures (6, 20 and 26 oC). There was no significant difference in the survival of E. coli in filtered versus non-filtered stream water. Lower water temperatures (6 oC) increased the survival of E. coli. The addition of manure to the water substantially increased the nutrient concentrations and organics. BST is a rapidly growing area of research and technology development and many methods are being developed and tested. The choice of method used for BST depends on: question(s) to be answered, scale of identification needed, available expertise, cost of analysis, turnaround time, and access to facilities. The spatial, diurnal, and annual sources and concentrations of E. coli were investigated in several watersheds in British Columbia. Fecal coliforms and E. coli concentrations varied throughout the day, as well as by site, month and year. Ribotyping identified many different sources of E. coli within the watersheds. The majority of E. coli isolates classified were from wildlife sources in each watershed even though they had different land-use.
Download or read book Microbial Source Tracking of Escherichia Coli in Lake Erie written by Richard J. Boheen and published by . This book was released on 2009 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: A microbial source tracking study was initiated to determine the sources of Escherichia coli in Lake Erie in western New York State. E. coli isolates were obtained from human and animal sources (deer, dog, cat, cow, goose, and gull) to create a library of known genetic fingerprints. E. coli were isolated from the sand and water at two recreational beaches on Lake Erie: Point Gratiot Park Beach, and Wright Park Beach. E. coli were also isolated from the local tributaries: Canadaway Creek, Crooked Brook, and Hyde Creek. Amplified-Fragment Length Polymorphisms (AFLP) were used to generate unique genetic fingerprints from all E. coli isolates. DNA fingerprints from known sources were compiled into libraries using Gel Compar II software. Jackknife analysis of the DNA fingerprinting library using maximum similarities yielded percentages of 92%, 91.2%, 75%, 93.9%, 91.9%, 100%,100%, and 94.9% for goose, dog, deer, human, cat, cow, gull, and sand respectively. Sand isolates were added to the DNA fingerprinting library based on high fidelity of the group. Genetic comparison of DNA fingerprinting from unknown isolates against the DNA fingerprint library was based on densiometric curve comparisons based on the Pearson correlation coefficient, and dendograms were constructed with the unweighted pair group method using arithmetic averages (UPGMA). Comparison of 294 DNA fingerprints from environmental E. coli isolates against the DNA fingerprint library based on maximum similarities indicated that most isolates (73.5%) were not genetically similar to host sources in the library (based on a match of 85% or greater). Interestingly, 26% of the E. coli isolates obtained from Wright Park Beach were genetically similar to isolates from shoreline sand. The results of this study suggest that most of the E. coli present in the beach waters, sand and tributaries are naturalized strains and are not the result of fecal pollution. -- Author abstract.
Download or read book Development of a Rapid Method for a Human Pollution Source Tracking Marker Using Enterococcus Surface Protein esp in E Faecium written by Lekha Satheesh Kumar and published by . This book was released on 2007 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Source Tracking Methods Applications and Case Studies written by Charles Hagedorn and published by Springer Science & Business Media. This book was released on 2011-06-08 with total page 645 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the origin of fecal pollution is essential in assessing potential health risks as well as for determining the actions necessary to remediate the quality of waters contaminated by fecal matter. As a result, microbial source tracking (MST) has emerged as a field that has evolved and diversified rapidly since the first approaches were described only a decade ago. In response to the emergence of MST, there have been three large multi-laboratory method comparison studies (two in the US and one in Europe), plus numerous workshops, book chapters, and review articles dedicated to synthesizing information on the topic. Furthermore, a federal (USEPA) guide document describing the uses and limitations of MST methods was published in 2005, and a book dedicated to MST as an emerging issue in food safety was published in 2007. These documents provide a collective body of literature on MST that is both conflicting and complementary, often repetitious, and difficult to condense and interpret. In addition, it does not reflect the current diversity of MST approaches with different organisms, newer methodologies such as quantitative PCR, and anthropogenic chemicals, nor does it embrace the scope of MST research being conducted around the world. The three editors of the book, all with extensive MST expertise, have developed chapters and invited authors who reflect the rich diversity and truly international scope of MST. The unifying theme throughout the book is the design of more standardized approaches to MST that include performance criteria (regardless of method or organism), plus recommendations for field study design and MST implementation. The editors intend that this book will serve as a valuable reference for all those who are involved with
Download or read book Microbial Source Tracking of E Coli in Storm Water Using the AFLP PCR Method written by Salam Daoud Assi and published by . This book was released on 2005 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Practical Methods to Assess the Presence of Bacterial Pathogens in Water written by W. A. Yanko and published by IWA Publishing. This book was released on 2004-03-01 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project was to develop and evaluate a practical technique to indicate the probability of the presence of bacterial pathogens in receiving waters. A practical method was defined as one that would limit the use of defined culture-based microbiological methods, and would be based on: (1) validating indicator organisms that predicted the presence of pathogens, or (2) detection of pathogens by polymerase chain reaction (PCR)-based assays. The study also assessed the utility of PCR-based technology for bacterial pathogen detection with respect to technology transfer to a wider range of water and wastewater facilities.
Download or read book Development Validation and Application of Molecular Microbial Source Tracking Methods to be Used in the Assessment of Environmental Waterways written by Richard Charles Sawdey and published by . This book was released on 2009 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Use of DNA Fingerprinting and Novel Molecular Methods to Identify Sources of Escherichia Coli in the Environment written by Troy Scott and published by . This book was released on 2019-05-31 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Microbial source tracking Dissertation Discovery Company and University of Florida are dedicated to making scholarly works more discoverable and accessible throughout the world. This dissertation, "Use of DNA Fingerprinting and Novel Molecular Methods to Identify Sources of Escherichia Coli in the Environment" by Troy M. Scott, was obtained from University of Florida and is being sold with permission from the author. A digital copy of this work may also be found in the university's institutional repository, IR@UF. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation.
Download or read book Anthropogenic Impacts on the Microbial Ecology and Function of Aquatic Environments written by Maurizio Labbate and published by Frontiers Media SA. This book was released on 2016-09-06 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aquatic ecosystems are currently experiencing unprecedented levels of impact from human activities including over-exploitation of resources, habitat destruction, pollution and the influence of climate change. The impacts of these activities on the microbial ecology of aquatic environments are only now beginning to be defined. One of the many implications of environmental degradation and climate change is the geographical expansion of disease- causing microbes such as those from the Vibrio genus. Elevating sea surface temperatures correlate with increasing Vibrio numbers and disease in marine animals (e.g. corals) and humans. Contamination of aquatic environments with heavy metals and other pollutants affects microbial ecology with downstream effects on biogeochemical cycles and nutrient turnover. Also of importance is the pollution of aquatic environments with antibiotics, resistance genes and the mobile genetic elements that house resistance genes from human and animal waste. Such contaminated environments act as a source of resistance genes long after an antibiotic has ceased being used in the community. Environments contaminated with mobile genetic elements that are adapted to human commensals and pathogens function to capture new resistance genes for potential reintroduction back into clinical environments. This research topic encompasses these diverse topics and describes the affect(s) of human activity on the microbial ecology and function in aquatic environments and, describes methods of restoration and for modelling disturbances.
Download or read book Microbial Source Tracking written by Thomas B. Atherholt and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
