Download or read book Development and Characterization of Cell Lines and Virus Expressing SV40 Small t Antigen written by Benette Phillips and published by . This book was released on 1988 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation and Characterization of Cell Lines Altered in the Expression of SV40 T antigen written by Vera Wolowodiuk Byrnes and published by . This book was released on 1988 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book CHARACTERIZATION OF AN SV40 TRANSFORMATION REVERTANT CELL LINE VIRUS written by GARY HOWARD SILVERSTEIN and published by . This book was released on 1991 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: respond to the additional large T antigen. Both the hierarchy of response to additional large T antigen and the normal growth phenotype of CL1L may be a reflection of the cellular alteration.
Download or read book DNA Tumor Viruses written by Blossom Damania and published by Springer Science & Business Media. This book was released on 2008-12-19 with total page 805 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book focuses on the DNA viruses in the human population that are associated with cancers. It covers most of the viruses that are thought to contribute to human malignancy. This book represents a comprehensive review of the field of DNA tumor virology. Right now, while there are books out there that cover individual viruses that are also covered in this book, there is no single book that covers this topic comprehensively. This book is the first current, comprehensive review of its kind in the market.
Download or read book Adeno Associated Virus AAV Vectors in Gene Therapy written by Kenneth I. Berns and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human gene therapy holds great promise for the cure of many genetic diseases. In order to achieve such a cure there are two requirements. First, the affected gene must be cloned, its se quence determined and its regulation adequately characterized. Second, a suitable vector for the delivery of a good copy of the affected gene must be available. For a vector to be of use several attributes are highly desirable: these include ability to carry the intact gene (although this may be either the genomic or the cDNA form) in a stable form, ability to introduce the gene into the desired cell type, ability to express the introduced gene in an appropriately regulated manner for an extended period of time, and a lack of toxicity for the recipient. Also of concern is the frequency of cell transformation and, in some cases, the ability to introduce the gene into nondividing stem cells. Sev eral animal viruses have been tested as potential vectors, but none has proven to have all the desired properties described above. For example, retroviruses are difficult to propagate in sufficient titers, do not integrate into nondividing cells, and are of concern because of their oncogenic properties in some hosts and because they integrate at many sites in the genome and, thus, are potentially insertional mutagens. Additionally, genes introduced by retroviral vectors are frequently expressed for relatively short periods of time. A second virus used as a vector in model systems has been adenovirus (Ad).
Download or read book Cell Line Development written by Mohamed Al-Rubeai and published by Springer Science & Business Media. This book was released on 2009-08-11 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
Download or read book Therapeutic Antibody Engineering written by William R Strohl and published by Elsevier. This book was released on 2012-10-16 with total page 697 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of antibody engineering has become a vital and integral part of making new, improved next generation therapeutic monoclonal antibodies, of which there are currently more than 300 in clinical trials across several therapeutic areas. Therapeutic antibody engineering examines all aspects of engineering monoclonal antibodies and analyses the effect that various genetic engineering approaches will have on future candidates. Chapters in the first part of the book provide an introduction to monoclonal antibodies, their discovery and development and the fundamental technologies used in their production. Following chapters cover a number of specific issues relating to different aspects of antibody engineering, including variable chain engineering, targets and mechanisms of action, classes of antibody and the use of antibody fragments, among many other topics. The last part of the book examines development issues, the interaction of human IgGs with non-human systems, and cell line development, before a conclusion looking at future issues affecting the field of therapeutic antibody engineering. - Goes beyond the standard engineering issues covered by most books and delves into structure-function relationships - Integration of knowledge across all areas of antibody engineering, development, and marketing - Discusses how current and future genetic engineering of cell lines will pave the way for much higher productivity
Download or read book Cellular Transforming Genes written by and published by . This book was released on 1986 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Viruses and Human Disease written by Ellen G. Strauss and published by Elsevier. This book was released on 2007-09-21 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised and updated, the new edition of this groundbreaking text integrates basic virology with pathophysiological conditions to examine the connection between virology and human disease. Most virology textbooks focus on the molecular biology involved without adequate reference to physiology. This text focuses on viruses that infect humans, domestic animals and vertebrates and is based on extensive course notes from James Strauss' virology class at the California Institute of Technology taught for over 30 years. Expertly depicting in color the molecular structure and replication of each virus, it provides an excellent overview for students and professionals interested in viruses as agents of human disease. - Includes over 30% new material - virtually all of the figures and tables have been redrawn to include the latest information and the text has been extensively rewritten to include the most up-to-date information - Includes a new chapter on emerging and reemerging viral diseases such as avian flu, SARS, the spread of West Nile virus across America, and the continuing spread of Nipah virus in Southeast Asia - Further reading sections at the end of each chapter make it easy find key references - World maps depicting the current distribution of existing and newly emerging viruses are also incorporated into the text
Download or read book Human Polyomaviruses written by Ugo Moens and published by Nova Science Pub Incorporated. This book was released on 2009 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human polyomaviruses are non-enveloped viruses with a circular double-stranded DNA genome of approximately 5,000 base pairs. The members BK virus and JC virus were first isolated in 1971 and are very common in the human population. Simian virus 40 (SV40), although originally isolated from rhesus macaques and accidentally introduced in the human population during massive poliovirus vaccination, may represent a third member of the polyomaviruses that can have man as a natural host. Primary infection with BK or/and JC virus occurs during early childhood. Subsequently, these viruses establish a seemingly life-long harmless latent infection. However, virus reactivation can occur especially in immunocompromised individuals and JC virus has been recognised as the cause of progressive multifocal leukoencephalopathy, while BK virus-associated interstitial nephritis has become an emerging complication in renal transplant patients. The genomes of human polyomaviruses encode two functional classes of proteins: the regulatory proteins large T-antigen, small t-antigen, and agnoprotein, and the structural proteins VP1, VP2 and VP3 that form the capsid. The regulatory proteins can modulate the expression and/or activity of cellular proteins involved in signal transduction, cell cycle regulation, and chromosomal functions. Therefore, they all possess oncogenic potential. Consistent with this, human polyomaviruses are capable of transforming human cells and inducing tumours in rodents, and are implicated in human malignancies. This book focuses on the etiological role of human polyomaviruses in cancer and highlights the mechanisms by which the viral regulatory proteins may contribute to neoplastic transformation of the infected host cells. Possible therapeutic strategies against these viruses will also be discussed.
Download or read book Small DNA Tumour Viruses written by Kevin Gaston and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In this timely book leading scientists from around the world review current hot-topics in this area providing a fascinating overview of the molecular biology of these viruses and their interactions with the host. Topics covered include: HPV infections and the production of HPV virion stocks; viral oncoproteins and their functions; the replication and maintenance of viral genomes; virus induced alterations in cellular miRNAs; viral deregulation of DNA damage responses; the initiation of viral DNA replication; induction of genomic instability by viral oncoproteins; targeting of PML proteins and.
Download or read book Immunobiotics Interactions of Beneficial Microbes with the Immune System written by Julio Villena and published by Frontiers Media SA. This book was released on 2018-01-26 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The term “immunobiotics” has been proposed to define microbial strains able to beneficially regulate the mucosal immune system. Research in immunobiotics has significantly evolved as researchers employed cutting-edge technologies to investigate the complex interactions of these beneficial microorganisms with the immune system. During the last decade, our understanding of immunobiotics-host interaction was profoundly transformed by the discovery of microbial molecules and host receptors involved in the modulation of gut associated immune system, as well as the systemic and distant mucosal immune systems. In recent years, there has been a substantial increase in the number of reports describing the beneficial effects of immunobiotics in diseases such as intestinal and respiratory infections, allergy, inflammatory bowel disease, obesity, immunosuppression, and several other immune-mediated conditions. Evidence is also emerging of immunobiotics related molecules with immunomodulatory functions leading to the production of pharmabiotics, which may positively influence human or animal health. Therefore, research in immunobiotics continue to contribute not only to food but also medical and pharmaceutical fields. The compilation of research articles included in this ebook should help reader to have an overview of the recent advances in immunobiotics.
Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Download or read book Immunization Safety Review written by Institute of Medicine and published by National Academies Press. This book was released on 2003-12-26 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Immunization Safety Review Committee was established by the Institute of Medicine (IOM) to evaluate the evidence on possible causal associations between immunizations and certain adverse outcomes, and to then present conclusions and recommendations. The committee's mandate also includes assessing the broader societal significance of these immunization safety issues. While all the committee members share the view that immunization is generally beneficial, none of them has a vested interest in the specific immunization safety issues that come before the group. The committee reviews three immunization safety review topics each year, addressing each one at a time. In this fifth report in a series, the committee examines the hypothesis that exposure to polio vaccine contaminated with simian virus 40 (SV40), a virus that causes inapparent infection in some monkeys, can cause certain types of cancer.
Download or read book Molecular Biology of Prostate Cancer written by Manfred Wirth and published by Walter de Gruyter. This book was released on 2013-05-22 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lentivirus Gene Engineering Protocols written by Maurizio Federico and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.
Download or read book Endothelial Cell Culture written by Roy Bicknell and published by Cambridge University Press. This book was released on 1996-09-28 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Endothelial Cell Culture contains chapters on endothelial cells derived from 1) lung, 2) bone marrow, 3) brain, 4) mammary glands, 5) skin, 6) adipose tissue, 7) female reproductive system, and 8) synovium.
