Download or read book Design Syntheses and Applications of Fluorescent Dyes written by Liangxing Wu and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: New methodologies for the efficient syntheses of 4,4-difluoro-4-bora-3a,4adiaza- s-indacenes (BODIPYs) and rosamines were developed. A serendipitous discovery led to a new reaction which afforded BODIPYs in high yields. Systematic studies of the kinetics and mechanisms of the new reaction were performed. A series of BODIPYs were successfully prepared using the new approach. A simple and efficient synthesis of rosamines with cyclic-amine substituents was devised. These new rosamines showed interesting anti-tumor activities. Several types of novel fluorescent compounds were prepared. Highly fluorescent GFP-chromophore analogs were designed and synthesized. The correlation between the optical properties and the structures was investigated. New pyronin dyes with mesoheteroatom substituents were efficiently prepared. The fluorescence properties of these compounds were highly dependent on the nature of the meso-substituents. A set of BODIPY dyes that fluoresce brightly above 600 nm were made. They were then used as acceptors to prepare water-soluble through-bond energy transfer cassettes. All the cassettes had complete energy transfer and high quantum yields in MeOH. A few also had good fluorescence properties in aqueous media and even on proteins. The through-bond energy transfer cassettes were used to monitor protein-protein interactions. In order to test our hypothesis, an artificial protein interaction system was built by utilizing the biotin/(strept)avidin interactions. Thus Atto425-BSA-biotin, streptavidin-cassette1 and avidin-cassette2 were prepared. The interactions between Atto425-BSA-biotin and cassette labeled (strept)avidin were successfully detected in vitro and in living cells by fluorescence techniques.

Download or read book Molecular Design and Synthesis of High Performance Fluorescent Dyes for Textile Applications written by Mohammed Saiful Islam and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescent Analogs of Biomolecular Building Blocks written by Marcus Wilhelmsson and published by John Wiley & Sons. This book was released on 2016-03-16 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.

Download or read book Design Synthesis and Characterization of Mechanosensitive Dual Fluorescent Dyes as Potential Probes for Biological Applications written by Susantha Ganegamage and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chapter 3 of this dissertation explores a hypothesis that leverages the seesaw photophysical model for N-aryl (2,3- and 1,8-) naphthalimides. The Heagy group initially developed this model to understand the dual fluorescence effect concerning the electron donor or electron-withdrawing substituents. This study envisions the design and synthesis of novel dual fluorescence molecules for a new class of N-aryl-phenanthridinone dyes. The applied photophysical model further investigates these dyes with a substitution pattern opposite naphthalimide systems. Interestingly, predictive computational modeling shows that the substituent pattern, used previously for 2,3-naphthalimides, relies on these groups getting placed on rings in opposite positions for N-aryl-phenanthridinones. Chapter 4, provides an overall conclusion of the and future directions for both parts of the research. In chapter 5, a complete description of experimental data is provided for the characterization of all the dye systems presented. This data includes a detailed synthesis procedure, characterization, purity analysis by HPLC, absorptivity calculation, fluorescence lifetime measurements, quantum yield determination, biological evaluation and fluorescence titration.

Download or read book Design and Synthesis of Fluorescent Dyes for Use in Proteomic Research written by Kevin James Spicka and published by . This book was released on 2008 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics is a rapidly developing field requiring powerful new technology in order to be able to detect proteins at increasingly lower concentrations. To aid in the detection of proteins at lower concentrations, DIGE dyes, a family of spectrally resolved fluorescent dyes, are currently available to proteomic researchers for 2D gel analysis. However, the demands of protein detection dictate that dyes that are even more sensitive and versatile be created. The syntheses of highly sensitive, water soluble BODIPY fluorophore dyes are described. These dyes are proposed to have the necessary sensitivity to allow for detection of proteins in much lower concentrations, providing an improvement over current protein detection limits. The BODIPY dyes that have been synthesized are available in a variety of absorbances and emissions. While fluorescent dyes that are amine-reactive are the most popular covalently binding protein labeling markers being used in today's proteomic research, thiol-reactive fluorescent markers are gaining importance in proteomic research. Since thiol residues are less common in proteins compared to their amine counterparts, saturation labeling and quantification are more easily achieved. The syntheses of sensitive thiol- reactive fluorescent dyes are described. These syntheses allow for quick generation of thiol-reactive fluorescent markers to be used in proteomic research.

Download or read book Design Synthesis and Application of Ultrastable Rylene Dyes for Fluorescent Labeling of Biomolecules written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of organic fluorescent dyes are experiencing a renaissance related to the increasing demands posed by new microscopy techniques for high resolution and high sensitivity. While in the last decade single molecule equipment and methodology has significantly advanced and in some cases reached theoretical limits (e.g. detectors approaching unity quantum yields) unstable emission from chromophores and photobleaching become more and more the bottleneck of the advancement and spreading of single-molecule fluorescence studies. The main goal of this work was the synthesis of fluorophores that are water-soluble, highly fluorescent in an aqueous environment, have a reactive group for attachment to a biomolecule and posses exceptional photostability. An approach towards highly fluorescent, water-soluble and monofunctional perylene-3,4,9,10-tetracarboxdiimide and terrylene-3,4:11,12-tetra carboxidiimide chromophores was presented. A new synthetic strategy for the desymmetrization of perylenetetracarboximides was elaborated; water-solubility was accomplished by introducing sulfonyl substituents in the phenoxy ring. Two strategies have been followed relying on either non-specific or site specific labeling. For this purpose a series of new water-soluble monofunctional perylene and terrylene dyes, bearing amine or carboxy group were prepared. The reactivity and photophysical properties of these new chromophores were studied in aqueous medium. The most suitable chromophores were further derivatized with amine or thiol reactive groups, suitable for chemical modification of proteins. The performance of the new fluorescent probes was assessed by single molecule enzyme tracking, in this case phospholipase acting on phospholipid supported layers. Phospholipase-1 (PLA-1) was labeled with N-hydroxysuccinimide ester functionalized perylene and terrylene derivatives. The purification of the conjugates was accomplished by novel convenient procedure for the removal of unreacted dye from.

Download or read book Design Synthesis and Application of Novel Conjugated Materials Part II written by Haichang Zhang and published by Frontiers Media SA. This book was released on 2021-11-09 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Near Infrared Applications in Biotechnology written by Ramesh Raghavachari and published by CRC Press. This book was released on 2020-06-16 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores developments in techniques in diagnostics, DNA sequencing, bioanalysis of immunoassays, and single-molecule detection. It promotes the measurement, identification, monitoring, analysis, and application of near-infrared spectroscopy (NIR) to medical and pharmaceutical advances. The text also considers noninvasive methods of NIR for successful, cost-effective, and prompt diagnoses of diseases.

Download or read book Industrial Dyes written by Klaus Hunger and published by John Wiley & Sons. This book was released on 2007-09-24 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: What would life be like without color? Ever since one can think back, color has always accompanied mankind. Dyes - originally obtained exclusively from natural sources - are today also produced synthetically on a large scale and represent one of the very mature and traditional sectors of the chemical industry. The present reference work on Industrial Dyes provides a comprehensive review of the chemistry, properties and applications of the most important groups of industrial dyes, including optical brighteners. It also outlines the latest developments in the area of functional dyes. Renowned experts in their respective fields have contributed to the chapters on chemical chromophores, synthesis and application of the various dye classes, textile dyeing and non-textile dyeing. The book is aimed at all professionals who are involved in the synthesis, production, manufacture or application of dyes and will prove to be an indispensable guide to all chemists, engineers and technicians in dye science and industry.

Download or read book Design Synthesis and Application of Novel Conjugated Materials written by Haichang Zhang and published by Frontiers Media SA. This book was released on 2021-02-11 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Synthesis of Fluorescent Probes written by Prabin Rai and published by . This book was released on 2013 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fundamental objective of this project is to design, synthesize, and characterize fluorescent dyes, which may be utilized in super resolution imaging techniques. In Chapters 1, 2 and 3, we concentrated on photoswitchable rhodamine dyes. We synthesized several rhodamine dyes and increased their water solubility, installed a bioconjugation unit and, more importantly, we optimized the absorption properties (close to 400 nm) of the rhodamine spirolactams in their closed state and studied their basic photophysical properties as well. In Chapter 4, we synthesized azido-DCDHF fluorogens that can be converted to the bright state after a 1,3-dipolar cycloaddition reaction between an azide-Ph-DCDHF and a strained alkene. We synthesized some strained alkenes, which may speed up the kinetics in 1,3-dipolar cycloaddition. This chemical method of turning the dyes from dark to bright state is a new dimension in the bioconjugation arena. In Chapter 5, we synthesized Nile red derivatives which can switch to a bright state from a dark state by collision on the cell surface utilizing PAINT methodology. We expected that the design of new Nile red derivatives may have better properties than the parent Nile red. Besides the PAINT technique, we worked on some active control of emission by enzymatic cleavage of fluorescent dyes in a dark state to the bright state, which can be utilized in super resolution imaging. Related to the 1,3-dipolar cycloaddition reaction between azido-DCDHF and norbornene, we have examined recently popularized tetrazine chemistry. We linked pyridyl tetrazines to DCDHF with short spacer. In Chapter 6, we describe the preparation of co-crystals between perfluorophenazine and several polynuclear aromatic compounds/polynuclear heteroaromatic compounds. In Chapter 7 we describe the preparation of some partially fluorinated heteropolynuclear aromatic compounds such phenzaine and acridine class of compounds for possible use in organic semiconductors.

Download or read book Design Synthesis and Application of Ultrastable Rylene Dyes for Fluorescent Labeling of Biomolecules written by Kalina Peneva and published by . This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Proceedings of the 2015 International Conference on Materials Engineering and Environmental Science MEES2015 written by International Conference on Materials Engineering and Environmental Science and published by World Scientific. This book was released on 2016 with total page 904 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book consists of one hundred and nine selected papers presented at the 2015 International Conference on Materials Engineering and Environmental Science (MEES2015), which was successfully held in Wuhan, China during September 25-27, 2015. All papers selected for this proceedings were subjected to a rigorous peer-review process by at least two independent peers. The papers were selected based on innovation, organization, and quality of presentation. The MEES2015 covered a wide spectrum of research topics, ranging from fundamental studies, technical innovations, to industrial applications in Chemical Material and Chemical Processing Technology, Composite Materials, Alloy Materials and Metal Materials, Characteristics of Materials, Building Material and Construction Technology, Ecology and Environment, Technology for Environmental Protection, Economy and Environment, Mechanical and Control Engineering, and Manufacturing Technology. The MEES2015 brought together more than one hundred researchers from China, South Korea, Taiwan, Japan, Malaysia, and Saudi Arabia, and provided them with a forum to share, exchange and discuss new scientific development and future directions of Materials Engineering and Environmental Science."--Provided by publisher

Download or read book written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Design and Syntheses of Dyes for Biological Applications written by Cliferson Thivierge and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The challenges in modern biological imaging applications are two-fold: (i) to develop better methods of imaging, and (ii) develop dyes that are suitable for these methods. This dissertation deals with the design and synthesis of dyes mainly by modification of known dyes to make them suitable for modern biological applications. Towards this aim, novel ways of derivatizing BODIPY dyes are explored. One method involves extending the conjugation via phenyl acetylene units, pushing fluorescence wavelengths near 600 nm. A different approach deals with C-H functionalization of BODIPY in which the fluors are functionalized with acrylate units, extending their fluorescence to the red. The BODIPY dyes developed are then incorporated in through-bond energy transfer cassettes. We examine the factors affecting energy transfer efficiencies by synthesizing analogs of the cassettes and also studying the electrochemical behavior of the donor and acceptor parts. The concept of through-bond energy transfer is incorporated into conjugated polymers by random incorporation of BODIPY dyes into polyfluorenes. The ideal ratio of fluorene to BODIPY parts was found to be 4:1. The BODIPY doping agents result in dispersed emissions when excited the polyfluorene polymers. Concurrently, the polyfluorene backbone acts as an energy harvester for the BODIPY dyes, in effect increasing their molar absorptivities. Finally the use of BODIPY dyes as photodynamic therapeutic agents was examined. We found that BODIPY dyes are efficient at producing reactive oxygen species when halogens are attached directly on the BODIPY core. Furthermore, the mechanism of cell death by using such agents was elucidated. Attachment of the most promising agent to polyglutamic acid is done to promote the EPR effect. Lastly we develop a potentially new type of PDT agent that absorbs strongly above 800 nm, permitting its use in deep tissue PDT.

Download or read book Systematic Exploration of Indolizine Based Small Fluorescent Molecules written by Youngjun Lee and published by Springer. This book was released on 2018-08-16 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes an in-depth study of an indolizine-based fluorophore, from understanding of its structure-photophysical property relationship to its application as a useful biological reporter. Organic fluorophores have been extensively used in the field of molecular biology owing to their excellent photophysical property, suitable cell permeability, and synthetic flexibility. Understanding of the structure-photophysical property relationship of a given fluorophore often paves the road to the development of valuable molecular probes to visualize and transcribe biological networks. In this thesis, respective chapters deal with molecular design, organic synthesis, structure-property analysis, and quantum-mechanical interpretation of unexplored family of indolizine-based molecules. This systematic exploration has led to rational development of a new microalgae lipid droplet probe, colorful bioorthogonal fluorogenic probes, and a bright mitochondrial probe, working under live cell conditions. Harnessing the optical properties of a given fluorophore has been an important topic for a couple of decades, both in industry and in academia. This thesis provides useful insights for the improvement and development of unique small fluorescent materials, or optical materials.

Download or read book Molecular Design and Synthesis of Coumarin Fluorescent Dyes written by Chih Hung Lui and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: