Download or read book Angiogenesis In Vivo Systems Part B written by and published by Academic Press. This book was released on 2009-02-12 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding how angiogenesis "works" and how to control it will have massive implications on the management, treatments, and ultimately the prevention of many common (and not so common) diseases. Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over angiogenesis. Diseases that are angiogenesis-dependent result when blood vessels either grow excessively or insufficiently. - Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition - Provides the "builder's manual" for essential techniques--a one-stop shop that eliminates needless searching among untested techniques - Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics
Download or read book Imaging and Spectroscopic Analysis of Living Cells written by P. Michael Conn and published by Academic Press. This book was released on 2012 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Publisher's note.
Download or read book Viral Polymerases and Related Proteins written by Lawrence C. Kuo and published by Elsevier. This book was released on 1996 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features* Expression, purification, and characterization* Activity assays* Kinetic and screening* Design and analysis of substrates and inhibitors* Molecular and structural characterizations.
Download or read book Fluorescence Spectroscopy written by and published by Academic Press. This book was released on 2011-09-06 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics. - Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique - Provides tried and tested techniques which eliminate searching through many different sources
Download or read book Protein Engineering for Therapeutics written by K. Dane Wittrup and published by Academic Press. This book was released on 2012 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike. Iinternational board of authors. This volume is split into sections that cover subjects such as Peptides, and Scaffolds
Download or read book Stem Cell Tools and Other Experimental Protocols written by and published by Elsevier. This book was released on 2006-12-12 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. - Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering - Presents the latest most innovative technologies - Addresses therapeutic relevance including FDA compliance and tissue engineering
Download or read book Computer Methods Part C written by and published by Academic Press. This book was released on 2010-12-24 with total page 701 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along with the 2 previous Computer Methods volumes for the Methods in Enzymology serial, aims to inform biomedical researchers about recent applications of modern data analysis and simulation methods as applied to biomedical research. - Presents step-by-step computer methods and discusses the techniques in detail to enable their implementation in solving a wide range of problems - Informs biomedical researchers of the modern data analysis methods that have developed alongside computer hardware - Presents methods at the "nuts and bolts" level to identify and resolve a problem and analyze what the results mean
Download or read book Oxygen Radicals in Biological Systems written by Lester Packer and published by Elsevier. This book was released on 1994 with total page 758 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. Key Features* Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or removal of oxygen radical* Methods for assessing molecular, cell, and tissue damage; assays and repair of oxidative damage.
Download or read book Energetics of Biological Macromolecules Part D written by Jo M. Holt and published by Elsevier. This book was released on 2004-04-02 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed.*Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis
Download or read book Chromatin and Chromatin Remodeling Enzymes Part B written by Carl Wu and published by Elsevier. This book was released on 2004-03-07 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.
Download or read book Methods in Enzymology written by Lester Packer and published by Gulf Professional Publishing. This book was released on 2005-08-18 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the inception of the series, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all field of life sciences. This final volume in the five-part Nitric Oxide series supplements MIE volumes 268, 269, 301 and 359. Nitric Oxide impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organic biology, as well as fundamental chemistry. These volumes are a valuable resource for the experienced researcher and for those entering the field. *One of the most highly respected publication in the field of biochemistry since 1955 *Frequently consulted and praised by researchers and reviewers alike *Truly an essential publication for anyone in any field of the life sciences
Download or read book Two Component Signaling Systems Part B written by and published by Academic Press. This book was released on 2007-07-03 with total page 646 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections in Two-Component Signaling Systems, Part B include: - Structural Approaches - Reconstitution of Heterogeneous Systems - Intracellular Methods and Assays - Genome-Wide Analyses of Two-Component Systems - Presents detailed protocols - Includes troubleshooting tips
Download or read book Liposomes Part E written by and published by Elsevier. This book was released on 2005-02-28 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part E is a continuation of previous Methods in Enzymology Liposome volumes A, B, C and D. - One of the most highly respected publications in the field of biochemistry since 1955 - Frequently consulted, and praised by researchers and reviewers alike - Truly an essential publication for anyone in any field of the life sciences
Download or read book Synthetic Biology written by Christopher A. Voigt and published by Academic Press. This book was released on 2011 with total page 557 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic biology encompasses a variety of different approaches, methodologies and disciplines and many different definitions exist. This volume covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation.
Download or read book Constitutive Activity in Receptors and Other Proteins Part A written by and published by Academic Press. This book was released on 2010-11-26 with total page 821 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods
Download or read book Cellulases written by Harry Gilbert and published by Academic Press. This book was released on 2012-05-29 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyse cellulolysis. This volume covers subjects such as 'the DNSA reducing assay for measuring cellulases', 'measuring processivity' and 'in situ cellulose detection with carbohydrate-binding modules'.
Download or read book Oxygen Sensing written by and published by Elsevier. This book was released on 2004-05-10 with total page 867 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields.
