Download or read book Culture of Immortalized Cells written by R. Ian Freshney and published by Wiley-Liss. This book was released on 1996-04-15 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a detailed, yet concise manual which provides the most up-to-date in vitro methods & safety procedures for the immortalization of primary cultures for various cell types. Each method is accompanied by step by step protocols & instructions.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Neuronal Cell Culture written by Shohreh Amini and published by Humana. This book was released on 2022-06-09 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.
Download or read book Culturing Life written by Hannah Landecker and published by Harvard University Press. This book was released on 2010-03-30 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: How did cells make the journey, one we take so much for granted, from their origin in living bodies to something that can be grown and manipulated on artificial media in the laboratory, a substantial biomass living outside a human body, plant, or animal? This is the question at the heart of Hannah Landecker's book. She shows how cell culture changed the way we think about such central questions of the human condition as individuality, hybridity, and even immortality and asks what it means that we can remove cells from the spatial and temporal constraints of the body and "harness them to human intention." Rather than focus on single discrete biotechnologies and their stories--embryonic stem cells, transgenic animals--Landecker documents and explores the wider genre of technique behind artificial forms of cellular life. She traces the lab culture common to all those stories, asking where it came from and what it means to our understanding of life, technology, and the increasingly blurry boundary between them. The technical culture of cells has transformed the meaning of the term "biological," as life becomes disembodied, distributed widely in space and time. Once we have a more specific grasp on how altering biology changes what it is to be biological, Landecker argues, we may be more prepared to answer the social questions that biotechnology is raising.
Download or read book The Immortal Life of Henrietta Lacks written by Rebecca Skloot and published by Crown. This book was released on 2010-02-02 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: #1 NEW YORK TIMES BESTSELLER • “The story of modern medicine and bioethics—and, indeed, race relations—is refracted beautifully, and movingly.”—Entertainment Weekly NOW A MAJOR MOTION PICTURE FROM HBO® STARRING OPRAH WINFREY AND ROSE BYRNE • ONE OF THE “MOST INFLUENTIAL” (CNN), “DEFINING” (LITHUB), AND “BEST” (THE PHILADELPHIA INQUIRER) BOOKS OF THE DECADE • ONE OF ESSENCE’S 50 MOST IMPACTFUL BLACK BOOKS OF THE PAST 50 YEARS • WINNER OF THE CHICAGO TRIBUNE HEARTLAND PRIZE FOR NONFICTION NAMED ONE OF THE BEST BOOKS OF THE YEAR BY The New York Times Book Review • Entertainment Weekly • O: The Oprah Magazine • NPR • Financial Times • New York • Independent (U.K.) • Times (U.K.) • Publishers Weekly • Library Journal • Kirkus Reviews • Booklist • Globe and Mail Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells—taken without her knowledge—became one of the most important tools in medicine: The first “immortal” human cells grown in culture, which are still alive today, though she has been dead for more than sixty years. HeLa cells were vital for developing the polio vaccine; uncovered secrets of cancer, viruses, and the atom bomb’s effects; helped lead to important advances like in vitro fertilization, cloning, and gene mapping; and have been bought and sold by the billions. Yet Henrietta Lacks remains virtually unknown, buried in an unmarked grave. Henrietta’s family did not learn of her “immortality” until more than twenty years after her death, when scientists investigating HeLa began using her husband and children in research without informed consent. And though the cells had launched a multimillion-dollar industry that sells human biological materials, her family never saw any of the profits. As Rebecca Skloot so brilliantly shows, the story of the Lacks family—past and present—is inextricably connected to the dark history of experimentation on African Americans, the birth of bioethics, and the legal battles over whether we control the stuff we are made of. Over the decade it took to uncover this story, Rebecca became enmeshed in the lives of the Lacks family—especially Henrietta’s daughter Deborah. Deborah was consumed with questions: Had scientists cloned her mother? Had they killed her to harvest her cells? And if her mother was so important to medicine, why couldn’t her children afford health insurance? Intimate in feeling, astonishing in scope, and impossible to put down, The Immortal Life of Henrietta Lacks captures the beauty and drama of scientific discovery, as well as its human consequences.
Download or read book The Impact of Food Bioactives on Health written by Kitty Verhoeckx and published by Springer. This book was released on 2015-04-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.
Download or read book Parkinson s Disease and Related Disorders written by Peter Riederer and published by Springer Science & Business Media. This book was released on 2006-11-23 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives a comprehensive overview on current clinical and basic research issues related to Parkinson’s disease and its related disorders. The book is the result of the 16th International Congress of Parkinson’s Disease and Related disorders congress 2005. Its contents are suitable for neurologists, psychiatrists, neurosurgeons, basic researchers, geneticians and patients as well as their relatives.
Download or read book Guide to Research Techniques in Neuroscience written by Matt Carter and published by Academic Press. This book was released on 2022-03-26 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Download or read book Cancer Cell Culture written by Simon P. Langdon and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.
Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Download or read book Cell Line Development written by Mohamed Al-Rubeai and published by Springer Science & Business Media. This book was released on 2009-08-11 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
Download or read book Bone and Cartilage Regeneration written by Phuc Van Pham and published by Springer. This book was released on 2017-01-24 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: This invaluable resource discusses clinical applications with effects and side-effects of applications of stem cells in bone and cartilage regeneration. Each chapter is contributed by a pre-eminent scientist in the field and covers such topics as skeletal regeneration by mesenchymal stem cells, clinical improvement of mesenchymal stem cell injection in injured cartilage and osteoarthritis, Good manufacturing practice (GMP), minimal critera of stem cells for clinical applications, future directions of the discussed therapies and much more. Bone & Cartilage Regeneration and the other books in the Stem Cells in Clinical Applications series will be invaluable to scientists, researchers, advanced students and clinicians working in stem cells, regenerative medicine or tissue engineering.
Download or read book Cell Culture Technology written by Cornelia Kasper and published by Springer. This book was released on 2018-10-10 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.
Download or read book Translational Biology in Medicine written by M. Montano and published by Elsevier. This book was released on 2014-12-08 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: The recent emphasis in biomedical research on translational biology and personalized medicine is revolutionizing conceptual and experimental approaches to understanding and improving human health. Translational Biology in Medicine begins with an introduction to experimental model systems for disease, such as cell lines, primary cells, stem cells and animal models for disease, followed by a systematic description of genetic and genomic profiling and biomarker validation currently used in biomedical research. Examples of translation studies that have used these models and methods are presented, including studies in aging, tissue repair and chronic infection, each with an emphasis on how personalized medicine is transforming biomedicine. Bioethical considerations in translational study design and bioethical considerations in biomedical research are then covered, before concluding remarks, and a look towards the future of personalized medicine. Describes cellular and animal model systems used in translational research Discusses the use of blood, genetic and genomic biomarkers for disease Presents translational studies in aging, tissue repair and infectious disease biomedicine
Download or read book Low Dose Radiation Effects on Animals and Ecosystems written by Manabu Fukumoto and published by Springer Nature. This book was released on 2019-11-14 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book summarizes the latest scientific findings regarding the biological effects of the Fukushima Daiichi Nuclear Power Plant (FNPP) accident in 2011. Various cases of changes in animals and organisms have been reported since the FNPP accident. However, it is often unknown whether they are actually due to radiation, since the dose or dose-rate are not necessarily associated with the changes observed. This book brings together the works of radiation biologists and ecologists to provide reliable radioecology data and gives insight into future radioprotection. The book examines the environmental pollution and radiation exposure, and contains valuable data from abandoned livestock in the ex-evacuation zone and from wild animals including invertebrates and vertebrates, aqueous and terrestrial animals, and plants that are subjected to long-term exposure in the area still affected by radiation. It also analyzes dose evaluation, and offers new perspectives gained from the accident, as well as an overview for future studies to promote radioprotection of humans and the ecosystem. Since the biological impact of radiation is influenced by various factors, it is difficult to scientifically define the effects of low-dose/low-dose-rate radiation. However, the detailed research data presented can be combined with the latest scientific and technological advances, such as artificial intelligence, to provide new insights in the future. This book is a unique and valuable resource for researchers, professionals and anyone interested in the impact of exposure to radiation or contamination with radioactive materials.
Download or read book Culture of Human Tumor Cells written by Roswitha Pfragner and published by John Wiley & Sons. This book was released on 2005-03-11 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ein neuer Band aus der 'Culture of Specialized Cells'-Reihe. Leserfreundlich aufgemacht. Er vermittelt spezifische praktische Details, wie man Medien und Reagenzien sowie Protokolle für Zellisolierung und Zellkultur präpariert. Logisch aufgebaut und nach spezifischen Tumoren gegliedert. Farbtafeln demonstrieren anschaulich Immunozytochemie und Fluoreszenz in situ Hybridisierung (FISH). Darüber hinaus beschreibt das Buch auch umfangreiche Sicherheitsvorkehrungen. Mit einer Vielzahl nützlicher Tipps. Mit einem Glossar zu ausgewählten Fachtermini. Enthält eine umfangreiche Liste mit Bezugsadressen von Ausrüstung und Zellkulturprodukten. Erläutert medikamentöse Behandlung, Auswahl, Differenzierung, Assays für die Untersuchung maligner Zellen sowie Risiken und Anwendungsmöglichkeiten.