Download or read book Crystallography of Protein Dynamics written by and published by Elsevier. This book was released on 2023-09-25 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approx.400 pagesApprox.400 pages

Download or read book Using X ray Crystallography for the Analysis of Protein Dynamics written by Elena J. Levin and published by . This book was released on 2008 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Time resolved Protein Dynamics Using X ray Crystallography and Optical Spectroscopy written by James Michael Baxter and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Principles of Protein X ray Crystallography written by Jan Drenth and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro ductions to their fields. These should serve the needs of one-semester or one-quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface to the Second Edition Since the publication of the previous edition in 1994, X-ray crystallography of proteins has advanced by improvements in existing techniques and by addition of new techniques.

Download or read book Macromolecular Crystallography written by Maria Armenia Carrondo and published by Springer. This book was released on 2011-12-01 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics. A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.

Download or read book Development of X Ray Methods for the Investigation of Protein Dynamics written by Alexander Michael Wolff and published by . This book was released on 2020 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Throughout history, methodological innovations have resulted in breakthroughs in our understanding of biology. Methods for determining static protein structures, as well as those for probing protein dynamics, are well-established. Nonetheless, visualizing molecules as dynamic entities that respond to their environment is still an outstanding challenge. Specifically, it is challenging to measure the spatial position of all the atoms within a molecule as a function of time. That challenge is the broad focus of this dissertation.In chapter one, I begin by diving into modern crystallographic techniques that enable one to solve protein structures from sub-micron-sized crystals. I compare and contrast two methods, serial crystallography and electron crystallography, asking how each technique affects the protein's structure. A primary factor differentiating these two methods is the temperature of the sample during the experiment. Despite this difference, both methods enable one to solve high-resolution structures from small crystals. This is advantageous for time-resolved experiments. Since there are fewer molecules in a small crystal, the perturbation is more uniform, which provides a clearer time-resolved signal. In chapter two, I investigate temperature-jumps as a generalized perturbation for resolving the energy landscape of proteins. In this work, I focus on solution scattering experiments, which allow one to examine large-scale perturbations to a protein, as well as changes in the solvent shell surrounding the molecule. By mutating selected residues, we inhibited specific protein motions. Comparing these mutants to the wild-type protein allowed us to resolve the motions driven by an infrared laser. Nonetheless, we wished to gain all-atom spatial resolution, which required us to perform a temperature-jump within the context of crystallography rather than solution scattering.In chapter three, I expand upon the temperature-jump detection method described in chapter two. By adapting this method to accommodate X-ray diffraction images, I demonstrate that we can detect temperature-jumps within a crystalline context. This is a crucial step in the development of a generalized perturbation for time-resolved crystallography. Given the timescale of the measurements, reading out the temperature directly from the X-ray data is the only effective way to track the sample's response. Thus, our method offers proof-of-principle that IR laser-based temperature-jumps are feasible for time-resolved crystallography. While measuring the diffuse scattering signal is useful for temperature-jump detection, the diffuse signal also holds the potential to inform our understanding of protein dynamics.In chapter four, I review the field of macromolecular diffuse scattering, as of late 2017. I begin by considering data collection practices, which requires extremely careful and controlled measurements. Then I examine different group's approaches to processing the data, as well as their models of the disorder that drives it. Finally, I consider the broader impact of diffuse analysis upon the field, ranging from the improvement of molecular dynamics forcefields to improved phasing and resolution extension. While these impacts hold exciting implications, it is clear that collecting high-quality is the first challenge to solve.In chapter five, I examine the challenges of collecting high-quality diffuse scattering from protein crystals. I describe how parasitic scattering can confound our ability to develop rigorous models of the crystalline disorder that gives rise to the diffuse signal. Then I work through experimental measures that we took to minimize parasitic scattering while maximizing diffuse scatter driven by protein motions.

Download or read book Protein Crystallography written by T. L. Blundell and published by Academic Press. This book was released on 1976-01-28 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Practical Protein Crystallography written by Duncan E. McRee and published by Academic Press. This book was released on 2012-12-02 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed for easy use by both new and experienced protein crystallographers, this much-needed book is for anyone interested in solving protein structures by the method of crystallography. It contains many examples ofactual experiments and data, including electron density maps. Computer methods and computer code samples are presented. Practical Protein Crystallography is loaded with new information on area detectors, synchrotron radiation techniques, and the latest computer methods, and features the XtalView software system. Graduate students and teachers in physical biochemistry and pharmaceutical researchers will find this text a timely and convenient aid.

Download or read book The Next Generation in Membrane Protein Structure Determination written by Isabel Moraes and published by Springer. This book was released on 2016-08-23 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.

Download or read book Structural Biology in Drug Discovery written by Jean-Paul Renaud and published by John Wiley & Sons. This book was released on 2020-01-09 with total page 1367 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins

Download or read book Crystals X rays and Proteins written by Dennis Sherwood and published by Oxford University Press. This book was released on 2011 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt: Information derived from X-ray crystal structures of biological molecules allows us to explain their functions in living organisms in extraordinary detail, and to develop drugs to treat disease. This book describes the principles and practice of X-ray diffraction as a key technique at the forefront of new discoveries in biology and medicine.

Download or read book Studies in protein crystallography and dynamics written by Piet Gros and published by . This book was released on 1990 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biomolecular Crystallography written by Bernhard Rupp and published by Garland Science. This book was released on 2009-10-20 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesizing over thirty years of advances into a comprehensive textbook, Biomolecular Crystallography describes the fundamentals, practices, and applications of protein crystallography. Illustrated in full-color by the author, the text describes mathematical and physical concepts in accessible and accurate language. Biomolecular Crystallography will be a valuable resource for advanced undergraduate and graduate students and practitioners in structural biology, crystallography, and structural bioinformatics.

Download or read book Dynamics of Proteins and Nucleic Acids written by J. Andrew McCammon and published by Cambridge University Press. This book was released on 1988-04-29 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a self-contained introduction to the theory of atomic motion in proteins and nucleic acids. An understanding of such motion is essential because it plays a crucially important role in biological activity. The authors, both of whom are well known for their work in this field, describe in detail the major theoretical methods that are likely to be useful in the computer-aided design of drugs, enzymes and other molecules. A variety of theoretical and experimental studies is described and these are critically analyzed to provide a comprehensive picture of dynamic aspects of biomolecular structure and function. The book will be of interest to graduate students and research workers in structural biochemistry (X-ray diffraction and NMR), theoretical chemistry (liquids and polymers), biophysics, enzymology, molecular biology, pharmaceutical chemistry, genetic engineering and biotechnology.

Download or read book Protein Crystallography written by Gwyndaf Evans and published by Royal Society of Chemistry. This book was released on 2018-06-21 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein crystallography has become vital to further understanding the structure and function of many complex biological systems. In recent years, structure determination has progressed tremendously however the quality of crystals and data sets can prevent the best results from being obtained. With contributions from world leading researchers whose software are used worldwide, this book provides a coherent approach on how to handle difficult crystallographic data and how to assess its quality. The chapters will cover all key aspects of protein crystallography, from instrumentation and data processing through to model building. This book also addresses challenges that protein crystallographers will face such as dealing with data from microcrystals and multi protein complexes. This book is ideal for both academics and researchers in industry looking for a comprehensive guide to protein crystallography.

Download or read book Crystallographic and Modeling Methods in Molecular Design written by Charles E. Bugg and published by . This book was released on 1990 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date review of forefront approaches used in structure-based drug design and protein engineering is intended for chemists, molecular biologists, pharmacologists, crystallographers, computer modelers, and graduate students interested in drug design and protein engineering. Leaders in protein crystallography, molecular dynamics, computer graphics, and molecular modeling report on their major contributions to this rapidly evolving field. Readers can expect to obtain an overall introduction to the major approaches that are now being pursued in structure-based drug design and protein engineering.

Download or read book Synchrotron Radiation Sources A Primer written by Herman Winick and published by World Scientific. This book was released on 1995-02-28 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specialists in the technical components of a synchrotron light source are usually well versed in their field and in the associated technical literature. However, with the rapid and continuing growth of synchrotron radiation research, and with new facilities coming online and being authorized for design and construction around the world, there is a need for a reference book that describes the various technical components of a synchrotron light source in a manner that will be useful to those who lack specialized technical background, but who have responsibility for some part of the design, construction, operation or development of such a facility. This would include technicians, engineers and physicists who have technical background in related fields but no specific experience with a synchrotron light source, and also project managers, laboratory directors, and government officials involved with synchrotron light facilities.College level scientific or engineering training or the equivalent experience is assumed and appropriate mathematics is used throughout. However, the basic concepts in each chapter are given in less technical language and a glossary of terms as well as an index will make the book useful for those with less technical training or experience.