Download or read book Characterization of the RIFIN Protein Family of the Malaria Parasite Plasmodium Falciparum Welch 1897 written by Michaela Petter and published by . This book was released on 2007 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization and Visualization of Protein Export in the Human Malaria Parasite Plasmodium Falciparum Welch 1897 written by Christof Grüring and published by . This book was released on 2011 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of the Protein Export Steps at the Parasite host Cell Interface of the Human Malaria Parasite Plasmodium Falciparum Welch 1897 written by Paolo Mesén-Ramírez and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of the Protein Export Steps at the Parasite host Cell Interface of the Human Malaria Parasite Plasmodium Falciparum Welch 1897 written by Paolo Mesén-Ramírez and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Adhesion Ligand Phosphorylation in the Malaria Parasite Plasmodium Falciparum Welch 1897 written by Klemens Engelberg and published by . This book was released on 2012 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Invasion related Proteins of the Malaria Parasite Plasmodium Falciparum Welch 1892 written by Louisa Wilcke and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biochemical and Immunological Characterization of Plasmodium Falciparum Welch 1897 Erythrocyte Membrane Protein PfEMP 1 Domains written by Yu-Shan Chia and published by . This book was released on 2006 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Global Screening and Characterization of Plasmodium Falciparum Welch 1897 Merozoite Putative Invasion related Proteins written by Ana Lidia Cabrera-Aguirre and published by . This book was released on 2010 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of a Puf Protein Family Member in Malaria Parasite Plasmodium Falciparum written by Xiaoying Liang and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of gene expression is important to cellular growth and differentiation of eukaryotes, as it plays key roles in determining the eventual expression levels and cellular localizations of a large number of proteins. RNA-binding proteins (RBPs) with conserved RNA-binding domains (RBDs) are key molecules in controlling gene expression. These conserved RBDs can specifically interact with target RNAs through specific sequences and structures to regulate mRNA stability, translation efficiency and subcellular localization. One of the well-studied RBPs is the Puf family RBPs. Members of the Puf family share a highly conserved Puf RBD, which typically consists of eight imperfect tandem repeats of ~36 amino acids (aa). Here I report the studies of two Puf members, PfPuf3 in Plasmodium falciparum and its ortholog PyPuf3 in Plasmodium yoelii. Both genes could not be disrupted, suggesting that they may be essential for the intraerythrocytic developmental cycle (IDC). A time-course study of both PfPuf3 mRNA and protein indicated that PfPuf3 was expressed during the entire IDC, with peak expression in early trophozoites. Cellular fractionation demonstrated that PfPuf3 preferentially partitioned to the nuclear than the cytoplasmic fractions. Interestingly, tagging of the endogenous of PfPuf3 and PyPuf3 with the green fluorescent protein showed that both proteins were limited to the nucleus, which is in contrast to the cytoplasmic localization of PfPuf1 and PfPuf2. Further, we found PfPuf3 co-localized with a well-known nucleolus maker PfNop1, demonstrating that PfPuf3 is a nucleolar protein. Using a parasite line with PfPuf3-tagged with PTP (ProtC-TEV-ProtA), we were able to affinity purify the PfPuf3 protein complex and the associated RNAs. Proteomic analysis of the PfPuf3 complex identified 32 proteins that are associated with the 60S ribosome subunit, suggesting that PfPuf3 might be involved in ribosomal biogenesis. RNA sequencing analysis of the purified PfPuf3 complex revealed significant enrichment of the 28S rRNA and ITS2 (internal transcribed spacer 2), implying that PfPuf3 may recognize and bind to these rRNA sequences. Taken together, these results demonstrated nucleolar localization of PfPuf3 and suggested an essential function of PfPuf3 in ribosomal biogenesis.

Download or read book Functional Analysis of Trophozoite and Schizont exported Proteins of the Human Malaria Parasite Plasmodium Falciparum Welch 1897 written by Jan Stäcker and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Visualization and Characterization of Endocytic Processes in the Human Malaria Parasite P Falciparum Welch 1897 written by Sven Flemming and published by . This book was released on 2015 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mycobacterium ulcerans written by Gerd Pluschke and published by Humana. This book was released on 2022-10-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.