Download or read book Characterization of the MRNA Binding Properties of Eukaryotic Initiation Factor EIF3 and the Phosphorylation of EIF4B written by Bradon James Fabbri and published by . This book was released on 1995 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of the Eukaryotic Initiation Factor 4B eiF4B written by Katherine Beglinger and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For my dissertation work, I have focused on dissecting the mechanism and regulation of mRNA selection and recruitment to the human ribosome. I was interested in human eIF4B, which is a heavily phosphorylated, 69 kDa protein that promotes the mRNA helicase activity of eIF4A and interacts with the 43S pre-initiation complex. In Chapter 1, I introduce the mechanism by which translation initiation is regulated, focusing on control by post-translation modifications during growth and cellular stress. In Chapter 2, I describe a quantitative mass spectrometry method to characterize the site-specific phosphorylation changes that occur on eIF4G and eIF4B in response to mTOR inhibition in HeLa R19 cells. I also describe the development of a system to express and purify affinity tagged proteins from HeLa R19 stable cell lines. In addition to characterizing eIF4B phosphorylation dynamics, in Chapter 3 I characterize a novel 40S ribosomal subunit binding domain in eIF4B. I then described a cell-based assay I used to link this 40S binding domain to eIF4B's ability to associate with an mRNA. Described in Chapter 4, this method utilizes live cell fluorescence microscopy to track the ability of eIF4E, eIF4G, and eIF4B to accumulate into stress granules during oxidative stress. In Chapter 5, I characterize the conformation of eIF4B using ion mobility mass spectrometry and show that phosphorylation at Ser422 does not change its structure. In the final chapter, I outline the methods used to generate Flp-in T-REx HeLa R19 cell lines to stably express modified eIF4G and eIF4B constructs that are used in various experiments performed in this dissertation. My dissertation work builds upon the advances made to characterize phosphorylation sites on eIF4B to provide important mechanistic insight into the additional layer of regulation phosphorylation provides to control protein synthesis.
Download or read book Eukaryotic Initiation Factor 4B eIF4B written by David Shahbazian and published by . This book was released on 2008 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of the Structure and Function of the alpha beta and gamma subunits of Eukaryotic Initiation Factor EIF 2 and Studies of EIF 4F from Mammalian Cells written by Bradley John Scherer and published by . This book was released on 1994 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Translation Initiation Reconstituted Systems and Biophysical Methods written by and published by Academic Press. This book was released on 2007-10-23 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.
Download or read book Physical Biochemistry written by David Freifelder and published by Macmillan. This book was released on 1982-08-15 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: Suitable for advanced undergraduate and graduate students in biochemistry, this book provides clear, concise, well-exampled descriptions of the physical methods that biochemists and molecular biologists use.
Download or read book Translational Control of Gene Expression written by Nahum Sonenberg and published by CSHL Press. This book was released on 2001 with total page 1034 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translation control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translation machinery of virus infection and disease. This book is essential reading for students entering the field and an invaluable resource for investigators of gene expression and its control.
Download or read book Examination of the Structure Function and Regulation of Eukaryotic Translation Initiation Factor 3 eIF3 written by Greg Leon Mayeur and published by . This book was released on 2001 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sp1 is Necessary for Maximal Transcription of the Eukaryotic Translation Initiation Factor 4b EIF4B Gene written by Dulce M. Alvarado and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eukaryotic translation initiation factor 4b (EIF4B) is a gene that encodes an RNA binding protein involved in the initiation step of protein synthesis. Phosphorylation and high protein levels of EIF4B have been associated with the development of tumorigenesis. Because EIF4B gene expression is essential for translation, the EIF4B putative promoter region was identified and characterized. [...]
Download or read book Systems Biology of Cancer written by Sam Thiagalingam and published by Cambridge University Press. This book was released on 2015-04-09 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: An overview of the current systems biology-based knowledge and the experimental approaches for deciphering the biological basis of cancer.
Download or read book Progressive Brain Disorders in Childhood written by Juan M. Pascual and published by Cambridge University Press. This book was released on 2017-04-20 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of childhood neurodegenerative and other progressive but non-degenerative disorders to guide their diagnosis and management.
Download or read book Translation and Its Regulation in Cancer Biology and Medicine written by Armen Parsyan and published by Springer. This book was released on 2014-10-13 with total page 709 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, for the first time, comprehensively assembles and analyzes a large body of information on the role of the fundamental mechanism of the protein biosynthesis pathway, translation, in cancer biology. It systematically explores the function of the translation machinery and its regulation, including cell signaling, in the development, maintenance and progression of human cancer. The work presented here unveils the tremendous potential and applications of this vast and exciting branch of genetic, biochemical and molecular science in cancer medicine and drug development. Chapters contributed by experts in the field take the reader on a journey that starts with a dissection of the translation machinery and its regulation in norm and cancer. Later chapters characterize etiological and pathogenetic roles that translation plays in specific cancer types. Various aspects of diagnostic, prognostic and therapeutic significance of the translation machinery and its control in cancer are discussed. Readers will discover the importance of the process of translation and its regulatory mechanisms in physiology and cancer biology. The chapters and the numerous illustrations included here were contributed by expert scientists and clinicians from renowned academic and clinical establishments in Canada, the United States of America, the United Kingdom, Italy, France, Belgium, Spain, Germany and Australia. The book conveys information and knowledge that may interest a broad range of students and scholars ranging from basic scientists to clinicians and drug developers seeking to better understand the protein synthesis and its aberrations in cancer biology and cancer medicine.
Download or read book Fluid Resuscitation written by Institute of Medicine and published by National Academies Press. This book was released on 1999-10-05 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Historically, 20% of all injured combatants die on the battlefield before they can be evacuated to a field hospital. Blood lossâ€"hemorrhageâ€"is the single major cause of death among those killed in action whose lives might otherwise be saved. Fluid resuscitation and the treatment of hypovolemia (the abnormally decreased volume of circulating fluid in the body) offer the greatest opportunity for reducing mortality and morbidity associated with battlefield casualties. In Fluid Resuscitation, a committee of experts assess current resuscitation fluids and protocols for the treatment of combat casualties and make recommendations for future research. Chapters focus on the pathophysiology of acute hemorrhagic shock, experience with and complications of fluid resuscitation, novel approaches to the treatment of shock, protocols of care at the site of injury, and future directions for research. The committee explicitly describes the similarities and differences between acute medical care during combat and civilian emergency trauma care. Fluid Resuscitation should help energize and focus research in both civilian and military emergency care and help save the lives of citizens and soldiers alike.
Download or read book Translation Initiation Extract Systems and Molecular Genetics written by and published by Academic Press. This book was released on 2007-10-15 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.
Download or read book MCQs in Biochemistry written by G. Vidya Sagar and published by New Age International. This book was released on 2008 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medical and Paramedical graduates aspiring for higher education planning to take PG ought to appear in entrance examinations. These entrance examinations are usually patterned in objective type. Biochemistry forms an integral part of curriculum of medical and paramedical courses. It is an important subject and deals with various Chemical, Biochemical, and Physiological reactions and processes that take place inside a living system. Quite a large number of MCQs appear in PG medical and paramedica.
Download or read book Chromatin Signaling and Diseases written by Olivier Binda and published by Academic Press. This book was released on 2016-08-06 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatin Signaling and Diseases covers the molecular mechanisms that regulate gene expression, which govern everything from embryonic development, growth, and human pathologies associated with aging, such as cancer. This book helps researchers learn about or keep up with the quickly expanding field of chromatin signaling. After reading this book, clinicians will be more capable of explaining the mechanisms of gene expression regulation to their patients to reassure them about new drug developments that target chromatin signaling mechanisms. For example, several epigenetic drugs that act on chromatin signaling factors are in clinical trials or even approved for usage in cancer treatments, Alzheimer's, and Huntington's diseases. Other epigenetic drugs are in development to regulate various class of chromatin signaling factors. To keep up with this changing landscape, clinicians and doctors will need to stay familiar with genetic advances that translate to clinical practice, such as chromatin signaling. Although sequencing of the human genome was completed over a decade ago and its structure investigated for nearly half a century, molecular mechanisms that regulate gene expression remain largely misunderstood. An emerging concept called chromatin signaling proposes that small protein domains recognize chemical modifications on the genome scaffolding histone proteins, facilitating the nucleation of enzymatic complexes at specific loci that then open up or shut down the access to genetic information, thereby regulating gene expression. The addition and removal of chemical modifications on histones, as well as the proteins that specifically recognize these, is reviewed in Chromatin Signaling and Diseases. Finally, the impact of gene expression defects associated with malfunctioning chromatin signaling is also explored. - Explains molecular mechanisms that regulate gene expression, which governs everything from embryonic development, growth, and human pathologies associated with aging - Educates clinicians and researchers about chromatin signaling, a molecular mechanism that is changing our understanding of human pathology - Explores the addition and removal of chemical modifications on histones, the proteins that specifically recognize these, and the impact of gene expression defects associated with malfunctioning chromatin signaling - Helps researchers learn about the quickly expanding field of chromatin signaling
Download or read book Tau Biology written by Akihiko Takashima and published by Springer Nature. This book was released on 2020-02-24 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents essential studies and cutting-edge research results on tau, which is attracting increasing interest as a target for the treatment of Alzheimer's disease. Tau is well known as a microtubule-associated protein that is predominantly localized in the axons of neurons. In various forms of brain disease, neuronal loss occurs, with deposition of hyperphosphorylated tau in the remaining neurons. Important questions remain regarding the way in which tau forms hyperphosphorylated and fibrillar deposits in neurons, and whether tau aggregation represents the toxic pathway leading to neuronal death. With the help of new technologies, researchers are now solving these long-standing questions. In this book, readers will find the latest expert knowledge on all aspects of tau biology, including the structure and role of the tau molecule, tau localization and function, the pathology, drivers, and markers of tauopathies, tau aggregation, and treatments targeting tau. Tau Biology will be an invaluable source of information and fresh ideas for those involved in the development of more effective therapies and for all who seek a better understanding of the biology of the aging brain.
