Download or read book Cell Surface Proteases written by and published by Elsevier. This book was released on 2003-05-03 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Surface Proteases provides a comprehensive overview of these important enzymes that catalyze the hydrolysis of a protein as it degrades to a simpler substance. In the 1990s, an explosion of new discoveries shed light on the role of cell surface proteases and extended it beyond degradation of extracellular matrix components to include its influence on growth factors, cell signaling, and other cellular events. This volume unites the scientific literature from across disciplines and teases out unified themes of interactions between cell surface proteases and interconnecting cell surface-related systems -- including integrins and other adhesion molecules. Scientists and students involved in developmental biology, cell biology and disease processes will find this an indispensable resource. * Provides an overview of the entire field of cell surface proteases in a single volume* Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine * A thematic volume in the longest-running forum for contemporary issues in developmental biology with over 30 years of coverage
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Activation of Viruses by Host Proteases written by Eva Böttcher-Friebertshäuser and published by Springer. This book was released on 2018-05-22 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will give an overview on viruses undergoing proteolytic activation through host proteases. The chapters will be organized in three themed parts, the first part describing respective viruses and their characteristics in detail. In the second part the molecular and cellular biology of the proteases involved as well as their physiological functions will be further explored. The third part will contain a chapter on protease inhibitors that are promising tools for antiviral therapy. This book will engage scholars in virology and medical microbiology as well as researchers with an interest in enzymology and protein structure and function relationship.
Download or read book TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades written by Wolfgang B. Liedtke, MD, PH.D. and published by CRC Press. This book was released on 2006-09-29 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first TRP ion channel was discovered in Drosophila melanogaster in 1989, the progress made in this area of signaling research has yielded findings that offer the potential to dramatically impact human health and wellness. Involved in gateway activity for all five of our senses, TRP channels have been shown to respond to a wide range of st
Download or read book Proteases Structure and Function written by Klaudia Brix and published by Springer Science & Business Media. This book was released on 2014-01-21 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteolysis is an irreversible posttranslational modification affecting each and every protein from its biosynthesis to its degradation. Limited proteolysis regulates targeting and activity throughout the lifetime of proteins. Balancing proteolysis is therefore crucial for physiological homeostasis. Control mechanisms include proteolytic maturation of zymogens resulting in active proteases and the shut down of proteolysis by counteracting endogenous protease inhibitors. Beyond the protein level, proteolytic enzymes are involved in key decisions during development that determine life and death – from single cells to adult individuals. In particular, we are becoming aware of the subtle role that proteases play in signaling events within proteolysis networks, in which the enzymes act synergistically and form alliances in a web-like fashion. Proteases come in different flavors. At least five families of mechanistically distinct enzymes and even more inhibitor families are known to date, many family members are still to be studied in detail. We have learned a lot about the diversity of the about 600 proteases in the human genome and begin to understand their physiological roles in the degradome. However, there are still many open questions regarding their actions in pathophysiology. It is in this area where the development of small molecule inhibitors as therapeutic agents is extremely promising. Approaching proteolysis as the most important, irreversible post-translational protein modification essentially requires an integrated effort of complementary research disciplines. In fact, proteolytic enzymes seem as diverse as the scientists working with these intriguing proteins. This book reflects the efforts of many in this exciting field of research where team and network formations are essential to move ahead.
Download or read book Monitoring Proteolytic Cleavage of a Membrane Metalloproteinase on the Cell Surface with a Cell based Assay written by and published by . This book was released on 2016 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteolysis is one of the most essential biological processes as it serves different purposes such as protein maturation, degradation, and regulation. Proteolysis at the cell surface and extracellular matrix (ECM) plays significant roles in the progression of certain diseases as exemplified by the role of Matrix Metalloproteinase 14 (MMP-14) in cancer. MMP-14 is a ubiquitously-expressed cell surface protease known to activate several soluble MMPs (EC-MMPs) via extracellular proteolysis. Active EC-MMPs are then used for the restructuring of the ECM that can facilitate cell migration and development. MMP-14 is known to be highly expressed in cancer tissues, as cells require constant remodeling of the ECM to accommodate fast replication and migration that leads to metastasis. The aim of this research is to develop a novel cell-based assay for the monitoring of proteolytic cleavage on the cell surface. We propose to develop such assay using MMP-14 cleavage activity as a proof-of-principle. The assay could also serve as a screening tool for the search of novel MMP-14 inhibitors. The assay is based on a two-tag system flanking an optimized substrate of MMP-14 that serves as the primary detection element that can distinguish cleavage and non-cleavage events. A fluorescent protein is later introduced as a visual marker for cell surface localization by fluorescence microscopy. Lastly, the entire scaffold protein will be targeted and anchored on the cell surface by the addition of the C-terminal transmembrane domain of mouse Lyt-2 cell surface protein. The utility of the assay for monitoring cleavage on the cell surface will be demonstrated with the overexpression of MMP-14 as a proof-of-principle. Once the assay is calibrated and optimized to function as intended, it could also be further used as a platform to monitor other biologically important surface proteolysis events such as cleavage of the Amyloid Precursor Protein (APP) in Alzheimer disease and proteolysis of sialic acid by the influenza virus neuraminidase (NA) for the spread of viral particles. More importantly, it can also be adapted to a high-throughput screening (HTS) platform for the screening for novel inhibitors of those aforementioned proteases.
Download or read book Characterization of Lymphoid Cell surface Proteases written by Robert Jerrold Fulton and published by . This book was released on 1980 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Download or read book Proteolytic Enzymes written by Erwin E. Sterchi and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following an overview on proteolytic enzyme assays, this text covers procedures on how to investigate and study proteases. It describes the use of specific restriction proteases as well as inhibitors of proteases to prevent unwanted proteolysis.
Download or read book Mast Cell Biology written by Alasdair M. Gilfillan and published by Springer Science & Business Media. This book was released on 2011-06-28 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The editors of Mast Cell Biology, Drs. Gilfillan and Metcalfe, have enlisted an outstanding group of investigators to discuss the emerging concepts in mast cell biology with respect to development of these cells, their homeostasis, their activation, as well as their roles in maintaining health on the one hand and on the other, their participation in disease.
Download or read book Tumour Cell Surface Proteases and Their Inhibitors written by Mohammad Anees and published by . This book was released on 1995 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Biology by the Numbers written by Ron Milo and published by Garland Science. This book was released on 2015-12-07 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Download or read book Proteases and Their Receptors in Inflammation written by Nathalie Vergnolle and published by Springer Science & Business Media. This book was released on 2011-08-03 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteases are everywhere from prokaryotes to eukaryotes, from virus to bacteria and in all human tissues, playing a role in many biological functions. Among these functions, the inflammatory reaction is of particular interest. In inflamed tissues, proteases can have a microbial and/or host origin and are involved not only in tissue remodeling, but also in specific signaling to resident or inflammatory cells, thereby contributing to the innate immune response. This volume presents all advances in our knowledge of the role proteases and their inhibitors play in various diseases associated with inflammatory response. Mechanisms involved in protease signaling to cells are presented, and the different types of proteases that are present at inflammatory sites and their effects on the course of inflammation are discussed. Finally, the evidence for considering proteases and their receptors as potential molecular targets for therapeutic interventions in the treatment of inflammatory diseases is discussed in the context of specific organ inflammatory pathologies (the lung, gastrointestinal tract, skin, joints, etc.).
Download or read book Proteases written by Vito Turk and published by Birkhauser. This book was released on 1999 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Glycosaminoglycans as Possible Modulators of Cell Surface Proteases written by Rita Yu Yin Yong and published by . This book was released on 1984 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Surfaceome written by Kenneth R. Boheler and published by Humana Press. This book was released on 2017-12-20 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.
Download or read book The Role of the Cell Surface Proteases Meprin A and B in Breast Cancer Progression written by and published by . This book was released on 1999 with total page 27 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progression of cancer cells from a non-invasive, non-metastatic phenotype to an invasive and metastatic phenotype is associated with the expression of a variety of cell surface proteases. Primary among these are the zinc metalloproteases known as matrixins, which include matrix metalloproteases such as collagenases and stromelysins. Matrixins can degrade extracellular matrix proteins, contributing to metastasis and angiogenesis. Another family of zinc metalloproteases are the astacins', which include the extracellular proteases meprin A and B. The expression of a novel form of meprin beta mRNA only in cancer cells, and the secretion of meprin A protein by colon carcinomas has been previously documented. This indicates that these proteases play a role in cancer cell progression. The current project focuses on the expression of meprins A and B in breast cancer cells, and their potential role in tumorigenesis, invasion and metastasis.
