Download or read book Cell Fate in the C Elegans Germline is Regulated by the CPEB Protein FOG 1 written by Suk-Won Jin and published by . This book was released on 2000 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book C Elegans FOG 1 CPEB and FOG 3 Tob Work Together to Control the Oogenesis Program written by and published by . This book was released on 2015 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Germ cell sex determination is an ancient cell fate decision, but the molecular regulation of this fundamental decision is poorly understood. In the C. elegans germline, FOG-1 and FOG-3 are the terminal regulators of sperm fate. Here I explore sex determination on a genome wide scale in the C. elegans germline. First, I report on a new dataset of spermatogenic, oogenic, and gender neutral mRNAs in the germline. Second, I incorporate the sperm, oocyte, and maternal transcriptome datasets into this new dataset to characterize these transcripts into oogenic and spermatogenic mRNA programs. Third, I identify 81 FOG-1 associated mRNAs and 722 FOG-3 associated mRNAs. 76 of the FOG-1 associated mRNAs are also FOG-3 associated mRNAs and most of these mRNAs are oogenic program mRNAs. FOG-1 and FOG-3 also co-localize in the germline and co-immunoprecipitate suggesting that FOG-1 and FOG-3 work together. I propose a model in which FOG-1 and FOG-3 are in a complex repressing oogenic mRNAs to specify sperm fate.
Download or read book Molecular Regulation of Germ Cell Number and Fate in the Nematode C Elegans written by Liana B. Lamont and published by . This book was released on 2006 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Control of C Elegans Development by the Conserved RNA Regulators FBF and FOG 1 written by Beth Ellen Thompson and published by . This book was released on 2006 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Post transcriptional Regulation of Spermatogenesis in Caenorhabditis Elegans written by Cameron M. Luitjens and published by . This book was released on 2000 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Germline Stem Cells written by Steven X. Hou and published by Humana Press. This book was released on 2014-10-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. The volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.
Download or read book Genomic Perspectives on Cell Fate Reprogramming in the Caenorhabditis Elegans Germline written by and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In multicellular organisms, genetic programs guide cells to adopt particular cell fates as tissues are formed during development, maintained in adults and repaired after injury. Cell fates can also be reprogrammed artificially by genetic or chemical manipulation. In Caenorhabditis elegans, germ cell fate can be chemically reprogrammed: mutant puf-8; lip-1 adult hermaphrodites make sperm but can be switched to oocyte production by treatment with a MEK inhibitor. Such chemical reprogramming is uncommon: out of six genetically diverse sperm-only mutant backgrounds, only one other mutant could undergo chemical reprogramming, fbf-1; lip-1. Reprogramming "competence" thus depends on genotype. In addition, we show that two genes encoding PUF family member RNA-binding proteins, puf-8 and fbf-1, are redundantly required for chemical reprogramming of germ cell fate. Next, genomic analysis of reprogramming competent worms revealed an enrichment of oogenic mRNAs relative to mutants lacking the competence for chemical reprogramming. This intermediate state also existed at the protein level, since puf-8;lip-1 and fbf-1; lip-1 mutants expressed proteins diagnostic of both sperm and oocyte. Therefore reprogramming competent mutants possess an intersexual molecular profile at both RNA and protein levels. This intersexual molecular signature may poise the germline tissue for changing its cellular sexual state in response to environmental cues.
Download or read book A Genetic and Molecular Analysis of C Elegans Puf 8 and Fbf 1 in Germline Sex Determination and Proliferation written by Jennifer L. Bachorik and published by . This book was released on 2005 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Control of Cell Fate Specification in Caenorhabditis Elegans Germline written by SRIVALLI MAMILLAPALLI and published by . This book was released on 2015 with total page 75 pages. Available in PDF, EPUB and Kindle. Book excerpt: The precise regulation of germ cell fates (sperm or oocyte) lies at the heart of reproduction and fertility. The nematode Caenorhabditis elegans hermaphrodites produce a discrete number of sperm during larval development and then switch to produce oocyte during adulthood. A number of positive (e.g., fbf genes) and negative (e.g., gld-3) regulators are important for this switch. Here, we found that aberrant activation of MPK-1 (an ERK homolog) by removal of both fbf-1 and lip-1 partially inhibits sperm-oocyte switch, resulting in Mog (masculinization of germline) sterility. The fbf-1 gene encodes a conserved PUF (Pumilio and FBF) RNA-binding protein and the lip-1 gene encodes an MPK-1/ERK phosphatase. Notably, inhibition of MPK-1/ERK signaling by either genetic mutation or chemical inhibition reprograms the germ cell fate and thus helps in regaining the fertility. We also found that fbf-1; lip-1 Mog sterility was enhanced by the depletion of G2/M cell cycle regulators, including CYB-3/Cyclin B, CDK-1/CDK1, and CDC-25.1/CDC25. Markedly, cdc-25.1 mRNA is a direct target of FBF-1. These results suggest that FBF-1 and LIP-1 may promote sperm-oocyte switch by activating MPK-1/ERK signaling and G2/M cell cycle progression.
Download or read book The C Elegans TPR Containing Protein TRD 1 Regulates Cell Fate Choice in the Developing Germ Line and Epidermis written by Samantha Hughes and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Signaling in Spermatogenesis and Male Infertility written by Rajender Singh and published by CRC Press. This book was released on 2019-11-06 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spermatogenesis involves the coordination of a number of signaling pathways, which culminate into production of sperm. Its failure results in male factor infertility, which can be due to hormonal, environmental, genetic or other unknown factors. This book includes chapters on most of the signaling pathways known to contribute to spermatogenesis. Latest research in germ cell signaling like the role of small RNAs in spermatogenesis is also discussed. This book aims to serve as a reference for both clinicians and researchers, explaining possible causes of infertility and exploring various treatment methods for management through the basic understanding of the role of molecular signaling. Key Features Discusses the signaling pathways that contribute to successful spermatogenesis Covers comprehensive information about Spermatogenesis at one place Explores the vital aspects of male fertility and infertility Explains the epigenetic regulation of germ cell development and fertility Highlights the translational opportunities in molecular signaling in testis
Download or read book Stem Cell Biology written by Daniel R. Marshak and published by CSHL Press. This book was released on 2001 with total page 566 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cells are the focus of intense interest from a growing, multidisciplinary community of investigators with new tools for isolating and characterizing these elusive cell types. This volume, which features contributions from many of the world's leading laboratories, provides a uniquely broad and authoritative basis for understanding the biology of stem cells and the current excitement about their potential for clinical exploitation. It is an essential work of reference for investigators in embryology, hematology, and neurobiology, and their potential for clinical exploitation. It is an essential work of reference for investigators in embryology, hematology, and neurobiology, and their collaborators in the emerging field of regenerative medicine.
Download or read book Germ Cell Development in C elegans written by Tim Schedl and published by Springer Science & Business Media. This book was released on 2012-08-09 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Germ cells in sexually reproducing metazoa, through the germline lineage, are the route by which genetic material and cytoplasmic constituents are passed from one generation to the next in the continuum of life. Chapters in this book review germ cell development in the model organism Caenorhabditis elegans, discussing the biology, the genetics and the molecular mechanisms for various processes, as well as drawing comparisons with other organisms. Processes discussed include specification of germ cell fate, meiosis, gametogenesis, environmental/ physiological controls, epigenetics and translational control, fertilization and the oocyte-to-embryo transition. This book thus provides a comprehensive picture of the germline lineage and the continuum of life for the worm.
Download or read book Transcriptional and Translational Regulation of Stem Cells written by Gary Hime and published by Springer Science & Business Media. This book was released on 2013-05-22 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes the latest findings on transcriptional and translational regulation of stem cells. Both transcriptional activators and repressors have been shown to be crucial for the maintenance of the stem cell state. A key element of stem cell maintenance is repression of differentiation factors or developmental genes – achieved transcriptionally, epigenetically by the Polycomb complex, and post-transcriptionally by RNA-binding proteins and microRNAs. This volume takes two approaches to this topic – (1) illustrating the general principles outlined above through a series of different stem cell examples – embryonic, iPS and adult stem cells, and (2) describing several molecular families that have been shown to have roles in regulation of multiple stem cell populations.
Download or read book Post Transcriptional Regulation by STAR Proteins written by Talila Volk and published by Springer Science & Business Media. This book was released on 2011-01-11 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the available information on the structure of the RNA binding STAR domain and provides insights into how these proteins discriminate between different RNA targets. It reviews what is known about STAR proteins and human disease.
Download or read book Sperm Biology written by Scott S. Pitnick and published by Academic Press. This book was released on 2008-11-21 with total page 683 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sperm Biology represents the first analysis of the evolutionary significance of sperm phenotypes and derived sperm traits and the possible selection pressures responsible for sperm-egg coevolution. An understanding of sperm evolution is fast developing and promises to shed light on many topics from basic reproductive biology to the evolutionary process itself as well as the sperm proteome, the sperm genome and the quantitative genetics of sperm. The Editors have identified 15 topics of current interest and biological significance to cover all aspects of this bizarre, fascinating and important subject. It comprises the most comprehensive and up-to-date review of the evolution of sperm and pointers for future research, written by experts in both sperm biology and evolutionary biology. The combination of evolution and sperm is a potent mix, and this is the definitive account. - The first review survey of this emerging field - Written by experts from a broad array of disciplines from the physiological and biomedical to the ecological and evolutionary - Sheds light on the intricacies of reproduction and the coevolution of sperm, egg and reproductive behavior
Download or read book Genetics of Sex Determination written by R.S. Verma and published by Elsevier. This book was released on 1996-04-23 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Genetical Theory of Natural Selection by R.A. Fisher (1930) dictated that sexual dimorphisms may depend upon a single medelian factor. This could be true for some species but his suggestion could not take off the ground as gender in Drosophila is determined by the number of X chromosomes. Technical advances in molecular biology have revived the initial thinking of Fisher and dictate that TDF or SRY genes in humans or Tdy in mice are sex determining genes. The fortuitous findings of XX males and XY female, which are generally termed sex reversal phenomenon, are quite bewildering traits that have caused much amazement concerning the pairing mechanism(s) of the pseudoautosomal regions of human X and Y chromosomes at meiosis. These findings have opened new avenues to explore further the genetic basis of sex determination at the single gene level.The aim of the fourth volume, titled Genetics of Sex Determination is to reflect on the latest advances and future investigative directions, encompassing 10 chapters. Commissioned several distinguished scientists, all pre-eminent authorities in each field to shed their thoughts concisely but epitomise their chapters with an extended bibliography. Obviously, during the past 60 years, the metoric advances are voluminous and to cover every account of genes, chromosomes, and sex in a single volume format would be a herculean task. Therefore, a few specific topics are chosen, which may be of great interest to scientists and clinicians. The seasoned scientists who love to inquire about the role of genes in sex determination should find the original work of these notable contributors very enlightening. This volume is intended for advanced students who want to keep abreast as well as for those who indulge in the search for genes of sex determination.
