Download or read book Cell Culture for Biochemists written by R.L.P. Adams and published by Elsevier. This book was released on 1990-10-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 2nd revised edition equals the popular 1st edition in providing a clear and detailed overview of cell culture. It presents information on: characteristics of cultured cells; culture vessels; glassware preparation and sterilisation techniques; subculturing; primary cells; cell culture media; techniques; contamination; the cell cycle; cell synchronisation; use of radioactive isotopes in cell culture; cell mutants and cell hybrids; viruses; and differentiation in cell cultures.Reviews on the 1st edition: ``.. the book provides an excellent insight into the way cell culture techniques can be employed in the analytical study of cellular biology.'' - Trends in Biochemical Sciences ``It is well written in a concise, easy-to-read style which stimulates the interest of the reader....'' - Science Tools ``A useful handbook on principles and practice.'' - Immunology Today

Download or read book Current Catalog written by National Library of Medicine (U.S.) and published by . This book was released on with total page 1712 pages. Available in PDF, EPUB and Kindle. Book excerpt: First multi-year cumulation covers six years: 1965-70.

Download or read book The Biochemistry of the Nucleic Acids written by R.L.P. Adams and published by Springer Science & Business Media. This book was released on 2013-04-18 with total page 689 pages. Available in PDF, EPUB and Kindle. Book excerpt: When the first edition of this book was published in 1950, it predated the publication of the double-helical structure of DNA by three years. It is not, therefore, surprizing that nothing of the original book remains in the current edition. Indeed, such is the pace of change in the field of nucleic acids that less than 50% of material incorporated into the 1986 edition has been retained. The book aims at the advanced undergraduate and at graduates that are undertaking course work or requiring an in-depth background for their research. It also aims to provide the established scientist with a single text that permits updating across the whole field from DNA structure, replication and repair, through gene expression and its control to protein synthesis. Every chapter is accompanied by thorough referencing that enables the reader to evaluate personally the data and methodology that cannot be included in the text. In an attempt to keep this list within bounds, references are limited to about ten per page and, to accommodate the more recent literature, many of the older references have been left out in this latest edition.

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by . This book was released on 1987 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cultured!cell - Biohazards - Sterilization - Cell!lines - Cloning - Specific!cell!types - Cell!separation - Transformed!phenotype - Cytotoxicity - Culture!of!specific!cell!types - Culture!of!tumor!tissue - Three!dimensional!culture!systems.

Download or read book Subcellular Biochemistry written by Donald B. Roodyn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this volume of SUBCELLULAR BIOCHEMISTRY we cover a wide range of topics of considerable biological importance and have continued in our policy of letting authors, rather than editors, decide the "natural" length of their articles. Thus, we have some short but nevertheless significant contributions, as well as more massive chapters. We start with a detailed account by 1. Oelze of the composition and development of the bacterial photosynthetic apparatus. A number of photosynthetic bacteria are discussed, with particular emphasis on the well-studied Rhodospirillum rubrum and Rhodopseudomonas sphae roides. The reader will no doubt be struck by the great wealth of information now available on the molecular organization of the photosynthetic and respi ratory systems in these organisms. Equally important is our improved under standing of the biosynthesis and assembly of these systems. It is now generally accepted that photosynthetic bacteria are excellent model systems for the study of bioenergetic processes. It may well be that they will become equally popular as models for the study of membrane biogenesis, and it is to be hoped that Oelze's erudite and comprehensive treatment of the subject will help in this regard.

Download or read book The Biochemistry of the Nucleic Acids written by R. L. Adams and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: When the first edition of this book was published in 1950, it set out to present an elementary outline of the state of knowledge of nucleic acid biochemistry at that time and it was the first monograph on the subject to appear since Levene's book on Nucleic Acids in 1931. The fact that a tenth edition is required after thirty five years and that virtually nothing of the original book has been retained is some measure of the speed with which knowledge has advanced in this field. As a result of this vast increase in information it becomes increasingly difficult to fulfil the aims of providing an introduction to nucleic acid biochemistry and satisfying the requirements of advanced undergraduates and postgraduates in biochemistry, genetics and molecular biology. We have attempted to achieve these aims by con centrating on those basic aspects not normally covered in the general biochemistry textbooks and by providing copious references so that details of methodology can readily be retrieved by those requiring further information. The first seven editions emerged from the pen of J. N. Davidson who died in September 1972 shortly after completing the seventh edition. The subsequent editions have been produced by various colleagues who have tried to retain something of the character and structure of the earlier editions while at the same time introducing new ideas and concepts and eliminating some of the more out -dated material.

Download or read book Cellular and Biochemical Science written by G. Tripathi and published by I. K. International Pvt Ltd. This book was released on 2010-03 with total page 1388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fundamental aim underlying Cellular and Biochemical Sciences is to emphasize diversified topics of current interest to postgraduate students pursuing different courses in the area of biological sciences including Zoology, Botany, Biochemistry and Biotechnology. The text is also relevant to the students of Life Sciences, Biosciences, Cell Biology, Bioengineering and Pharmacology. A total of 58 topics have been incorporated in the book and some of the topics are rarely found in other books of Biology. New information has been introduced which updates existing knowledge and enables the book to justify its claim as the most comprehensive text in the sphere of cellular and biochemical sciences at the postgraduate and competitive examination levels. Each and every chapter has been designed in lucid and readable manner. There are references, suggested readings, long questions and objective questions at the end of chapters for revision of topics.

Download or read book Comprehensive Biotechnology written by and published by Newnes. This book was released on 2011-08-26 with total page 5304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Comprehensive Biotechnology, Six Volume Set continues the tradition of the first inclusive work on this dynamic field with up-to-date and essential entries on the principles and practice of biotechnology. The integration of the latest relevant science and industry practice with fundamental biotechnology concepts is presented with entries from internationally recognized world leaders in their given fields. With two volumes covering basic fundamentals, and four volumes of applications, from environmental biotechnology and safety to medical biotechnology and healthcare, this work serves the needs of newcomers as well as established experts combining the latest relevant science and industry practice in a manageable format. It is a multi-authored work, written by experts and vetted by a prestigious advisory board and group of volume editors who are biotechnology innovators and educators with international influence. All six volumes are published at the same time, not as a series; this is not a conventional encyclopedia but a symbiotic integration of brief articles on established topics and longer chapters on new emerging areas. Hyperlinks provide sources of extensive additional related information; material authored and edited by world-renown experts in all aspects of the broad multidisciplinary field of biotechnology Scope and nature of the work are vetted by a prestigious International Advisory Board including three Nobel laureates Each article carries a glossary and a professional summary of the authors indicating their appropriate credentials An extensive index for the entire publication gives a complete list of the many topics treated in the increasingly expanding field

Download or read book Microcarrier cell culture principles and methods written by and published by . This book was released on with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Cell Culture Secondary MetabolismToward Industrial Application written by Frank DiCosmo and published by CRC Press. This book was released on 1996-07-10 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell cultures are used extensively in studies of secondary metabolism, for the biosynthesis of pharmaceuticals, flavors, essences, and pigments. This book highlights recent developments in the in vitro growth of cultured plant cells and in the production of valuable secondary metabolites. Plant Cell Culture Secondary Metabolism details research on many exciting areas including:

Download or read book Tissue Culture Techniques written by Bernice M. Martin and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi • Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods • Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Download or read book Annual Plant Reviews Biochemistry of Plant Secondary Metabolism written by Michael Wink and published by John Wiley & Sons. This book was released on 2011-06-13 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: This brand new Annual Plant Reviews volume is the second edition of the highly successful and well-received Annual Plant Reviews, Volume 2. This exciting new volume provides an up-to-date survey of the biochemistry and physiology of plant secondary metabolism. The volume commences with an overview of the biochemistry, physiology and function of secondary metabolism, followed by detailed reviews of the major groups of secondary metabolites: alkaloids and betalains, cyanogenic glucosides, glucosinolates and nonprotein amino acids, phenyl propanoids and related phenolics, terpenoids, cardiac glycosides and saponins. A final chapter discusses the evolution of secondary metabolism. This carefully compiled new edition brings together chapters from some of the world's leading experts in plant secondary metabolism. Completely revised and brought right up to date with much new information, this volume is an essential purchase for advanced students, researchers and professionals in biochemistry, physiology, molecular biology, genetics, plant sciences, agriculture, medicine, pharmacology and pharmacy, working in the academic and industrial sectors, including those working in the pesticide and pharmaceutical industries. Libraries in all universities and research establishments where these subjects are studied and taught will need copies of this excellent volume on their shelves. A companion volume Annual Plant Reviews Volume 39, Functions and Biotechnology of Plant Secondary Metabolites, Second Edition, Edited by M. Wink, is also available.

Download or read book Advances in Bioartificial Materials and Tissue Engineering Research and Application 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 829 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Bioartificial Materials and Tissue Engineering Research and Application / 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Bioartificial Materials and Tissue Engi. The editors have built Advances in Bioartificial Materials and Tissue Engineering Research and Application / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Bioartificial Materials and Tissue Engi in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Bioartificial Materials and Tissue Engineering Research and Application / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Download or read book Astrocytes Pt 3 Biochemistry Physiology and Pharmacology of Astrocytes written by Sergey Fedorff and published by Elsevier. This book was released on 2012-12-02 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Astrocytes: Cell Biology and Pathology of Astrocytes, Volume 3 deals with the involvement of astrocytes in neurological and neuropathological states of brain function. This volume discusses the expression of astrocyte-specific immunological markers and plasma membrane structures; the dynamics of the cytoskeleton and cell cycle; and the reactivity to injury and response of astrocytes in selected pathological and genetic states. The text also aims to inspire new ideas and research on astrocyte functions as well as on the pathogenesis of neurological disorders. Neuroscientists, cell biologists, clinicians, neuropathologists, and pediatric neurologists will find the book invaluable.

Download or read book Plant Cell and Tissue Culture written by Indra K. Vasil and published by Springer Science & Business Media. This book was released on 1994-06-30 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Download or read book Recent Progress of Biochemical and Biomedical Engineering in Japan II written by Takeshi Kobayashi and published by Springer Science & Business Media. This book was released on 2004-07-21 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: The areas we deal with in biochemical engineering have expanded to include many various organisms and humans. This book has gathered together the information of these expanded areas in biochemical engineering in Japan. These two volumes are composed of 15 chapters on microbial cultivation techniques, metabolic engineering, recombinant protein production by transgenic avian cells to biomedical engineering including tissue engineering and cancer therapy. Hopefully, these volumes will give readers a glimpse of the past and also a view of what may happen in biochemical engineering in Japan.

Download or read book Muscle and Meat Biochemistry written by A.M. Pearson and published by Elsevier. This book was released on 2012-12-02 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Muscle and Meat Biochemistry teaches the different concepts and topics under the eponymous subject. The book covers the gross and detailed composition and structure of muscles and the relationship of the nervous system with the muscular system; muscle cell differentiation and growth; proteins of the thick filament; and the molecular structure and enzymatic activity of myosin. The text also discusses the proteins found in the thin filament - actin, troponin, and myosin; skeletal muscle growth; protein metabolism; and fiber types. The book also encompasses cardiac and smooth muscle; sarcoplasmic proteins; the connective tissues - collagen, elastin, and ground substance; and the postmortem changes during conversion of muscle to meat. The text is recommended for advanced undergraduate and graduate students, as well as for scientists who would like to know more about muscle biology, muscle physiology, and meat science.